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A cloture is a method for ending a debate and taking a vote. In the  U.S., invoking cloture requires a three-fifths vote of the entire  Senate.
The Senate votes on the petition; three-fifths of the whole number of Senators (sixty if there are no vacancies) is the required majority; however, when cloture is invoked on a question of changing the rules of the Senate, two-thirds of the Senators voting (not necessarily two-thirds of all Senators) is the requisite majority.