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Differences between colonies: Religious: The Pilgrims who settled at Plymouth were... Puritans vs. Anglicans at Jamestown. Plus, Plymouth was founded for religious reasons while Jamestown was founded for economic reasons.
Basically both these regions, Plymouth and Jamestown had their locals as Indians. The differences, however, were those of the people who later on came to the region. In Jamestown, it was Europeans and in Plymouth it was Pilgrims. In Jamestown the conflicts were on the economical issue while in Plymouth, it were on economy and religion as well.