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Plymouth was colder, whereas Jamestown was warmer and more humid, and therefore more prone to harbor diseases. I'm sure that there were a lot more differences, but those are the big ones that leapt into my mind.
Differences between colonies: Religious: The Pilgrims who settled at Plymouth were... Puritans vs. Anglicans at Jamestown. Plus, Plymouth was founded for religious reasons while Jamestown was founded for economic reasons.