You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
Restriction enzymes are enzymes that can cut DNA. You can think of restriction enzymes as molecular scissors. DNA ligase is an enzyme that can join two DNA molecules. You can think of DNA ligase as molecular glue. DNA ligase joins two DNA molecules together by forming a phosphodiester bond between the two molecules.