1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
The Prophecy Of Azor Ahai. Cometh the hour, cometh the need for something, or someone, to put an end to the biggest threat of all. That someone is known as Azor Ahai, as told by an ancient prophecy and constantly hinted at by Red Priestess Melisandre (seriously, sometimes I feel that's all she talks about).