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If you take medicine (diabetes pills or insulin), you will probably want to know how well that medicine is working. The general rule is to test before meals and keep a record. If you want to know how your meals affect your blood sugar, testing about 2 hours after eating can be helpful.
Again, if you are non-insulin dependent and are wondering when is the best time to take your blood glucose levels, it is 1-2 hours after a meal. Generally speaking, if your fasting blood sugar and blood sugar before meals are OK, it doesn't mean your diabetes is under control.