1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
EditWho Is Melisandre? Melisandre, also known as the Red Woman, is a mysterious character whose motivations are not completely clear. She is a Red Priestess of the Lord of Light, a god figure who is worshipped prominently in the lands beyond Westeros. Like just a handful of other characters in the series, Melisandre seems to have actual magical powers. Although by her own admittance, many of her abilities are actually parlor tricks and illusions.