1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
In Greek mythology, Melinoe is the goddess of ghosts. She is the daughter of Persephone and Hades. She lives in the Underworld. She has a pale complexion and a short temper and takes after her father in almost every way, except from her beauty that she gets from her mother Persephone. Melinoe has the powers to turn any mortal insane by her phantoms. Little is known about Melinoe, but ancient Greeks used to conduct rituals and acts of praises to her as a sign of devotion and to garner protection from ghosts. This short article about religion can be made longer.