You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
You can think of restriction enzymes as little molecular scissors that slide along the DNA and cut the sugar-phosphate backbone wherever they find their restriction site. The figure shows how a restriction enzyme can make a cut in a circular piece of DNA and turn it into a linear piece.