You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
Let's look at a specific example to see how this works. The restriction enzyme EcoRI recognizes the sequence GAATTC. Whenever EcoRI sees this particular six-nucleotide sequence, it's going to make a cut in the DNA. Recall that DNA is always read from the 5' end to the 3' end of the strand.