You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
It shows where restriction enzymes cut the original piece of DNA. 1. 10. On the gel picture below, (a) circle the smallest fragment produced by a restriction enzyme and label it âsmallest.â. (b) circle the largest fragment produced by a restriction enzyme and label it âlargest.â. 11.