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The hypothalamus is a brain structure made up of distinct nuclei as well as less anatomically distinct areas. It is found in all vertebrate nervous systems. In mammals, magnocellular neurosecretory cells in the paraventricular nucleus and the supraoptic nucleus of the hypothalamus produce neurohypophysial hormones, oxytocin and vasopressin. These hormones are released into the blood in the posterior pituitary.
Various nuclei of the hypothalamus including the paraventricular nucleus (PVN), medial preoptic area (MPA), arcuate nucleus (AN), median eminence, suprachiasmatic nucleus (SCN), and the substantia nigra (SN) were microdissected by the Palkovits's micropunch technique (MohanKumar et al.