You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
A restriction enzyme is a protein that recognizes a specific, short nucleotide sequence and cuts the DNA only at that specific site, which is known as restriction site or target sequence.More than 400 restriction enzymes have been isolated from the bacteria that manufacture them. The number of cuts in an organism's DNA made by a particular restriction enzyme is determined by the number of restriction sites specific to that enzyme in that organism's DNA. 2  A fragment of DNA produced by a pair of adjacent cuts is called a RESTRICTION FRAGMENT.