1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Cast interviews reveal more. Redditor and moderator of /r/asoiaf BryndenBFish put together an overview of Melisandre's known character attributions for a discussion on the red priestess. Included in the round-up is a quote from Oliver Ford Davies, the actor who played Cressen.