1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Melisandre or Melisandre of Asshai is a shadowbinder from the distant country and city of Asshai, acting in the service of King Stannis Baratheon. She is also a priestess of the Lord of Light or Red God, R'hllor. She was sold into slavery as a child. Her name was once Melony.