1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Posted On: 20 Mar, 2015. Jewel Belair Staite is a very beautiful, talented, and charming Canadian actress who has taken Hollywood industry. She is one of the actresses in Hollywood who has been able to win millions of peopleâs heart all over the world. She was born on 2nd June, 1982 so this makes her age 32 years old.