You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
The restriction enzyme recognises a unique sequence of nucleotides in the DNA strand, which is usually between four to six base-pairs in length. The complimentary DNA strand has the same sequence but in the reverse direction, thus ensuring both strands of DNA are cut at the same location.