1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Mel is truly a lover of all people, and has never met a stranger. Mel has traveled to over 30 countries conducting miracle crusades and teaching others to release Godâs anointing for instant manifestations of miracles. In doing so he has seen countless miracles and witnessed over 900,000 people accept Jesus Christ as Lord. Mel is a published author, anointed Pastor and leader.