You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
This specific 6-nucleotide sequence is the restriction site of EcoRI (other restriction enzymes have other restriction sites). The green line in the figure indicates the path of the cut created by the enzyme. But restriction enzymes will cut not only the DNA and RNA of viruses invading a bacterium, but also DNA and RNA of any kind.