You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
Each restriction enzyme cuts DNA only where a specific sequence of base pairs occurs. For example, these are the sequences cut by the restriction enzymes BamHI and XmaI.: If we cut DNA from two different sources with the same restriction enzyme, DNA fragments with complementary sticky ends are formed: If the DNA fragments are mixed together, they will join together at random, provided their unpaired bases match.