You will need the following items: 1  Temporary Use Permit Application (completely filled out). If the applicant is not the owner of the subject property, provide a Letter of Authorization with original signature. 2  Burden of Proof.
This is known as enzyme digestion. On double stranded DNA the recognition sequence is on both strands, but runs in opposite directions. This allows the enzyme to cut both strands. Sometimes the cut is blunt, sometimes the cut is uneven with dangling nucleotides on one of the two strands.
Electrophoresis. Restriction enzymes are used to cut DNA into fragments. Solutions containing these fragments are placed on the surface of a gel to which an electric curâ¦rent is applied. The electric current causes the DNA fragments to move through the gel.