1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
Melinoe is an unusual goddess, and her appearance is frightening. Her left side is black and hardened like a mummy, to represent her father, the dark god of the Underworld, Hades. Her right side is pale and chalky as if she were drained of all her blood, to show the bright Persephone, her mother.