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knot noun [C] (FASTENING). âº a âfastening made by âtying together a âpiece or âpieces of âstring, ârope, âcloth, etc.: Wrap this âstring around the âpackage and then âtie a knot. fig. Sheâs so ânervous, her âstomach is in knots (= âfeels âtight and âuncomfortable).
Medical Definition of knot. 1  1: an interlacing of the parts of one or more flexible bodies (as threads or sutures) in a lump to prevent their spontaneous separationâsee surgeon's knot. 2  2: a usually firm or hard lump, swelling, or protuberance (as in a muscle or on the surface of a bone) or process a knot in a gland a bone with two or three knots.