1 Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Melisandre is a red priestess of R'hllor and a shadowbinder, hailing from the city of Asshai in the Further East of Essos. She has joined the entourage of Stannis Baratheon, Lord of Dragonstone, believing him to be Azor Ahai reborn, a hero destined to defeat the Great Other.
While the latest episode of Thrones ended the show's three-week kid-killing streak, it. made up for the combo-breaker by having us witness the priestess. Melisandre give birth to a shadow baby. A shadow baby. Clearly, it's a bit more complicated than that, but it's strange and horrific to behold all the same.