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Function and structure. Enzymes are very efficient catalysts for biochemical reactions. They speed up reactions by providing an alternative reaction pathway of lower activation energy. Like all catalysts, enzymes take part in the reaction - that is how they provide an alternative reaction pathway.
Temperature. As the temperature rises, reacting molecules have more and more kinetic energy. This increases the chances of a successful collision and so the rate increases. There is a certain temperature at which an enzyme's catalytic activity is at its greatest (see graph).This optimal temperature is usually around human body temperature (37.5 o C) for the enzymes in human cells.See graph) Provided that the substrate concentration is high and that temperature and pH are kept constant, the rate of reaction is proportional to the enzyme concentration. (See graph) Inhibition of enzyme activity Some substances reduce or even stop the catalytic activity of enzymes in biochemical reactions.