The DNA is then labeled on one strand. The fragments are then broken using the Maxam-Gilbert sequencing reagents. A control is run which is treated identically except it consists of the same promoter DNA without the transcription complex attached. The fragmented DNA is then separated on a gel.
Because of Turkey's geographical conditions, one can not speak about a general overall climate. In Istanbul and around the sea of Marmara (Marmara region) the climate is moderate (winter 4 deg.C and summer 27 deg.C); in winter the temperature can drop below zero.
1 The mean annual temperature in Istanbul, Turkey is mild at 14.1 degrees Celsius (57.4 degrees Fahrenheit). 2  Average monthly temperatures have a variation of 18 C (32.4 F) which is a slightly low range. 3  The range/ variation of diurnal mean temperatures is 7.8 C (14 F).