The DNA is then labeled on one strand. The fragments are then broken using the Maxam-Gilbert sequencing reagents. A control is run which is treated identically except it consists of the same promoter DNA without the transcription complex attached. The fragmented DNA is then separated on a gel.
Because of Turkey's geographical conditions, one can not speak about a general overall climate. In Istanbul and around the sea of Marmara (Marmara region) the climate is moderate (winter 4 deg.C and summer 27 deg.C); in winter the temperature can drop below zero.
In Western Anatolia (Aegean region) there is a mild Mediterranean climate with average temperatures of 9 deg.C in winter and 29 deg.C in summer. On the southern coast of Anatolia (Mediterranean region) the same climate can be found.