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Answers. Best Answer: That is precisely what it requires as also denoted by the word oxidative phosphorylation. It happens through the electron transport chain process and the proton gradient it creates. Substrate level phosphorylation does not require oxygen, it occurs during glycolysis and the Krebs cycle.Source(s): greendawn Â· 7 years ago.t happens through the electron transport chain process and the proton gradient it creates. Substrate level phosphorylation does not require oxygen, it occurs during glycolysis and the Krebs cycle. Source(s): greendawn Â· 7 years ago.
ATP synthase then uses the energy stored in this gradient to make ATP. This process is called oxidative phosphorylation because oxygen is the final electron acceptor and the energy released by reducing oxygen to water is used to phosphorylate ADP and generate ATP.