The DNA is then labeled on one strand. The fragments are then broken using the Maxam-Gilbert sequencing reagents. A control is run which is treated identically except it consists of the same promoter DNA without the transcription complex attached. The fragmented DNA is then separated on a gel.
Because of Turkey's geographical conditions, one can not speak about a general overall climate. In Istanbul and around the sea of Marmara (Marmara region) the climate is moderate (winter 4 deg.C and summer 27 deg.C); in winter the temperature can drop below zero.
Although Turkey is situated in large Mediterranean geographical location where climatic conditions are quite temperate, diverse nature of the landscape, and the existence in particular of the mountains that run parallel to the coasts, result in significant differences in climatic conditions from one region to the other.