Infection is rare, but has been reported in individuals with predisposing conditions. Here we describe the case of a woman with pyelonephritis and bacteremia in which Lactobacillus delbrueckii was determined to be the causative agent.
you should mix the alkaline cleaning solution with hot water and apply it to the travertine tile floor with a soft scrubbing sponge do not use any type of aggressive scrub pad or scrub brush on you travertine tile floor this is because travertine is a dense stone but the finish can easily be marred by using aggressive scrub pads or brushes soft white scrubbing brushes work great to scrub the travertine and the grout lines without damaging the polished or honed finish of your travertine tile floor
Wash it off with some warm water and soap and pat it dry. You could even scrub it with an old toothbrush. Some stones have rough patches on them that will make painting a little more difficult. You can sand it down with sandpaper, starting with 100 grit and moving to 150 and 220 grit until the patch is gone.