The Half Windsor Knot Tying Instructions. 1  1. Start with the wide end of the tie on the right and the small end on the left. 2  The tip of the small end should rest slightly above your belly-button (this will vary depending on your height and the length & thickness of your tie).
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. It is commonly abbreviated as EtBr , which is also an abbreviation for bromoethane.
Wear gloves from here on, ethidium bromide is a mutagen! 1  Add 5 Âµl ethidium bromide (from a stock solution of 10 mg/ml) per 100 ml gel solution for a final concentration of 0.5 Âµg/ml. Be very careful when handling the concentrated stock. 2  Stir the solution to disperse the ethidium bromide, then pour it into the gel rack.