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The quad screen is usually done between 15 and 20 weeks of pregnancy. You'll have a blood sample taken and sent to a lab for analysis. Results are usually available in about a week. The test measures the levels of four substances in your blood: 1  alpha-fetoprotein (AFP), a protein made by the baby.
The quad screen â also known as the quadruple marker test, the second trimester screen or simply the quad test â is a prenatal test that measures levels of four substances in a pregnant woman's blood: 1  Alpha-fetoprotein (AFP), a protein made by the developing baby. 2  Human chorionic gonadotropin (HCG), a hormone made by the placenta.