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DESCRIPTION Types of blood culture bottles and blood volumes required: 1  Yellow top â paediatric aerobic (0.5â4 mL blood). 2  Green top â adult aerobic (5â10 mL blood). 3  Orange top â anaerobic (5â10 mL blood). 4  Black top â mycobacteria (5â10 mL blood). 5  Silver top â mycoplasma (3â5 mL blood).
Remove the caps from one aerobic blood culture bottle and one anaerobic blood culture bottle. Clean each rubber septum with an alcohol prep. Prepare the 20 mL syringe and perform the venipuncture,drawing 20 mL in adult patients. A smaller draw volume,1-3 mL, is sufficient for pediatric patients.