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The quad screen is usually done between 15 and 20 weeks of pregnancy. You'll have a blood sample taken and sent to a lab for analysis. Results are usually available in about a week. The test measures the levels of four substances in your blood: 1  alpha-fetoprotein (AFP), a protein made by the baby.
An ultrasound scan is the only way to confirm that you're pregnant with twins. The earliest you're likely to find out is between 10 weeks and 13 weeks plus six days, when you have your dating scan (NCCWCH 2011, McAslan Fraser 2012). Ultrasound is almost foolproof at diagnosing twins. However, if you're carrying more than two, it gets harder to tell how many babies you're expecting.