The Half Windsor Knot Tying Instructions. 1  1. Start with the wide end of the tie on the right and the small end on the left. 2  The tip of the small end should rest slightly above your belly-button (this will vary depending on your height and the length & thickness of your tie).
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. It is commonly abbreviated as EtBr , which is also an abbreviation for bromoethane.
Add 5 Âµl ethidium bromide (from a stock solution of 10 mg/ml) per 100 ml gel solution for a final concentration of 0.5 Âµg/ml. Be very careful when handling the concentrated stock. Some researchers prefer not to add ethidium bromide to the gel itself, instead soaking the gel in an ethidium bromide solution after running, in this case the ethidium bromide solution has final concentration of 0.5 Âµg/ml.