Other infections can also cause stiff neck symptoms, such as meningococcal disease, an infection in the cervical spine. Any time a stiff neck is accompanied by a fever, it is advisable to seek immediate medical attention to check for these possibilities.ther infections can also cause stiff neck symptoms, such as meningococcal disease, an infection in the cervical spine. Any time a stiff neck is accompanied by a fever, it is advisable to seek immediate medical attention to check for these possibilities.
To prevent the formation of primer dimers, it is also important to ensure that the 3â ends are not complementary. 2.5 Distance between primers. The primers are designed so that the distance. from the end of F2 to the end of B2 (the region. amplified by the LAMP method) is between 120.
One Compared To Many. Another major difference between continuous and discontinuous synthesis is the number of primers for each. A primer is a short stretch of RNA that is bound to the unzipped DNA strands. This stretch of RNA is the starting line for DNA Polymerase delta to climb on and start copying.