The Half Windsor Knot Tying Instructions. 1  1. Start with the wide end of the tie on the right and the small end on the left. 2  The tip of the small end should rest slightly above your belly-button (this will vary depending on your height and the length & thickness of your tie).
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. It is commonly abbreviated as EtBr , which is also an abbreviation for bromoethane.
It requires 2-3 times more DNA compared to ethidium bromide, is a temporary stain, binds to phosphate groups, requires multiple destainings, is very time consuming, and the ban sharpness is mediocre because the gel also absorbs the stain (poor contrast)