Other infections can also cause stiff neck symptoms, such as meningococcal disease, an infection in the cervical spine. Any time a stiff neck is accompanied by a fever, it is advisable to seek immediate medical attention to check for these possibilities.ther infections can also cause stiff neck symptoms, such as meningococcal disease, an infection in the cervical spine. Any time a stiff neck is accompanied by a fever, it is advisable to seek immediate medical attention to check for these possibilities.
To prevent the formation of primer dimers, it is also important to ensure that the 3â ends are not complementary. 2.5 Distance between primers. The primers are designed so that the distance. from the end of F2 to the end of B2 (the region. amplified by the LAMP method) is between 120.
Primer Design Tips & Tools. Good primer design is essential for a successful PCR reaction. There are many factors to take into account when designing the optimal primers for your gene of interest. Here are some tips to consider when designing primers. In general, a length of 18â30 nucleotides for primers is good. Try to make the melting temperature (Tm) of the primers between 65Â°C and 75Â°C, and within 5Â°C of each other. If the Tm of your primer is very low, try to find a sequence with more GC content, or extend the length of the primer a little. Aim for the GC content to be between 40 and 60%, with the 3' of a primer ending in C or G to promote binding.