The Half Windsor Knot Tying Instructions. 1  1. Start with the wide end of the tie on the right and the small end on the left. 2  The tip of the small end should rest slightly above your belly-button (this will vary depending on your height and the length & thickness of your tie).
Ethidium bromide is an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. It is commonly abbreviated as EtBr , which is also an abbreviation for bromoethane.
Add Ethidium bromide to the liquid gel (1-5Âµl of a 10mg/ml stock solution per 100 ml of agarose gel). Allow the agarose to cool to 50 o C to 65 o C. If you donât it will warp the gel boxes.