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(1) The reason Jefferson choose the expression separation of church and state was because he was addressing a Baptist congregation; a denomination of which he was not a member. Jefferson wanted to remove all fears that the state would make dictates to the church.
The clause is cited by advocates of separation of church and state as an example of  original intent  of the Framers of the Constitution of avoiding any entanglement between church and state, or involving the government in any way as a determiner of religious beliefs or practices.