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DESCRIPTION Types of blood culture bottles and blood volumes required: 1  Yellow top â paediatric aerobic (0.5â4 mL blood). 2  Green top â adult aerobic (5â10 mL blood). 3  Orange top â anaerobic (5â10 mL blood). 4  Black top â mycobacteria (5â10 mL blood). 5  Silver top â mycoplasma (3â5 mL blood).
Venipuncture is the technique of choice for obtaining blood for culture. Arterial blood cultures are not associated with a higher diagnostic yields than venous blood cultures and are not recommended. Blood culture bottles are used for collection. If other blood work is to be collected, collect the blood cultures first. - Collect 2 SETS (4 bottles) from 2 separate sites, e.g. right arm and left arm. - Each set consists of a green (aerobic) and orange (anaerobic) bottle. - Always collect the green bottle first.