Other infections can also cause stiff neck symptoms, such as meningococcal disease, an infection in the cervical spine. Any time a stiff neck is accompanied by a fever, it is advisable to seek immediate medical attention to check for these possibilities.ther infections can also cause stiff neck symptoms, such as meningococcal disease, an infection in the cervical spine. Any time a stiff neck is accompanied by a fever, it is advisable to seek immediate medical attention to check for these possibilities.
To prevent the formation of primer dimers, it is also important to ensure that the 3â ends are not complementary. 2.5 Distance between primers. The primers are designed so that the distance. from the end of F2 to the end of B2 (the region. amplified by the LAMP method) is between 120.
Since DNA polymerase operates from the 5' (phosphate) to the 3' (sugar) end, this means that two different DNA primers are needed--one for each 5' end of side of the molecule. It is this requirement for dual primers that makes it possible to precisely define the area of a DNA molecule to be amplified by the PCR.