Salt has a molar mass of 58.44 g/mol, glucose of 180.15 g/mol. 50 grams of glucose results in 278 millimoles of glucose. 9 g of sodium chloride correspond to 154 millimoles of sodium chloride. The osmolarity of the solution of sodium chloride, however, is 308 milliosmoles / liter.
In the natural world DNA usually becomes available by death and lysis of other cells, but in the laboratory it is provided by the researcher, often as a genetically engineered fragment or plasmid. During uptake, DNA is transported across the cell membrane(s) , and the cell wall if one is present.
Transfecting HEK 293 Cells. Use this procedure to transfect plasmid DNA into HEK 293 cells in a 24-well format (for other formats, see Scaling Up or Down Transfections, below). All amounts and volumes are given on a per well basis. The day before transfection, trypsinize and count the cells. Plate 0.5 -1.25x105 cells per well in 0.5 ml of complete growth medium. Cell density should be 50-80% confluent on the day of transfection. (Optional) The day of transfection, remove growth medium from cells and replace with 0.5 ml of complete growth medium.