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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
The objective itself is usually a cylinder containing one or more lenses that are typically made of glass; its function is to collect light from the sample. Microscope objectives are characterized by two parameters: magnification and numerical aperture. The magnification typically ranges from 4Ã to 100Ã.