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The Cincinnati Zoo & Botanical Garden opens daily at 10am. Closing Times & Seasonal Schedule. January 3 â March 11: 10am â 5pm. March 12 â May 27: 10am â 5pm (early entry at 9am for members & ZAT ticket holders) May 28 â September 5: 10am â 6pm (early entry at 9am for members & ZAT ticket holders)
The Zoo also operates a guest shuttle that runs on a continuous loop from the Bus Lot to the bottom of the Zoo and back. Shuttle hours can be found on the Accessibility page. The shuttle takes 15 to 20 minutes to complete the loop and does not stop at Parking Lot C. Look for the shuttle icon on the Zoo map.