Hypothalamus releases corticotropin-releasing hormone (CRH) ---> Pituitary gland releases adrenocorticotropic hormone (ACTH) ---> ACTH stimulates the adrenal cortex ---> adrenal cortex releases cortisol into the blood stream ---> cortisol affects target cells, and ceases once homeostasis is reached.
A bacteria, which possesses capsule is a capsulated bacteria. The purpose of capsule staining is to observe bacterial capsule by distinguishing capsular material from the bacteria cell. Principle: In capsule staining, a smear of bacteria is made at the center of a slide.
For this reason we use a NEGATIVE STAIN to visualize them. That is, we use a stain which stains the background against which the uncolored capsule can be seen. Our procedure, the Gin's Method, uses india ink to color the background and crystal violet to stain the bacterial cell body.