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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
The maximum magnification of light microscopes is usually Ã1500, and their maximum resolution is 200nm, due to the wavelength of light. An advantage of the light microscope is that it can be used to view a variety of samples, including whole living organisms or sections of larger plants and animals.