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The innermost layer of a blood vessel wall is the tunica intima (too'ni-ka in-ti'ma; intimus = inmost), or tunica interna. It is composed of an endothelium (a simple squamous epithelium lining the blood vessel lumen) and a subendothelial layer made up of a thin layer of areolar connective tissue. The tunica media (me'de-a; medius = middle) is the middle layer of the vessel wall. It is composed of circularly arranged layers of smooth muscle cells. Sympathetic innervation causes the smooth muscle to contract, resulting in vasoconstriction (va'so-kon-strik'shun), or narrowing of the blood vessel lumen.
The wall of the heart is composed of three distinct layers. 2. The outermost layer, the epicardium, is made up of connective tissue and epithelium, and houses blood and lymph capillaries along with coronary arteries. 3. The middle myocardium consists of cardiac muscle and is the thickest layer of the heart wall. 4. The inner endocardium is smooth and is made up of connective tissue and epithelium, and is continuous with the endothelium of major vessels joining the heart. a. The endocardium houses Purkinje fibers.