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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
SUMMARY. 1  Various microscopic techniques generate different views of the cell and have different resolutions. The limit of resolution of a light microscope is about 0.2 Î¼m; of a transmission electron microscope, about 0.1 nm; and of a scanning electron microscope, about 10 nm. Standard (bright-field) light microscopy is best for stained or colored cells or tissue sections.