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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
On the chart below, notice that of all the structures listed, only the plant cell is within our resolution--just barely. The light microscope. The light microscope has a limit of resolution of about 200 nm (0.2 Âµm). This limit is due to the wavelength of light (0.4-0.7 Âµm). Cells observed under a light microscope can be alive, or fixed and stained. Image courtesy of WebPath.