Hypothalamus releases corticotropin-releasing hormone (CRH) ---> Pituitary gland releases adrenocorticotropic hormone (ACTH) ---> ACTH stimulates the adrenal cortex ---> adrenal cortex releases cortisol into the blood stream ---> cortisol affects target cells, and ceases once homeostasis is reached.
A bacteria, which possesses capsule is a capsulated bacteria. The purpose of capsule staining is to observe bacterial capsule by distinguishing capsular material from the bacteria cell. Principle: In capsule staining, a smear of bacteria is made at the center of a slide.
Procedure of Capsule Staining. 1  Place a small drop of a negative stain (India Ink, Congo Red, Nigrosin, or Eosin) on the slide. 2  Using sterile technique, add a loopful of bacterial culture to slide, smearing it in the dye. 3  Use the other slide to drag the ink-cell mixture into a thin film along the first slide and let stand for 5-7 minutes.