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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
Objective Lenses. The pattern of light formed by the specimen is focused into a real image by the objective lenses. When the proper illumination is provided the resolving power of a microscope depends on the quality of its objective lenses. There are four of them with magnifications of 4X, 10X, 40X, and 100X.