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The numerical aperture of a microscope objective defines the objective's resolution. Each microscope objective has a minimum and maximum magnification necessary for the details in an image to be resolved. A simple formula for the minimum value is (500 x NA). And for the maximum magnification (1000 x NA).
With the light microscope this is approximately half the wavelength of light used in illumination. Only with a shorter wavelength can greater resolution be achieved, as in the ELECTRON MICROSCOPE which gives a resolution of about 0.5 nm.