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The two key measures in P300 are amplitude and latency. The amplitude of the P300 response is proportional to the amount of attention devoted to the task and to the degree of information processing required, while the latency is considered a measure of stimulus classification speed.he presentation of these stimuli is unevenly alternated, and the subject must respond only to the infrequent âtargetâ stimulus rather than the frequent âstandardâ stimulus. The P300 refers to a spike in activity approximately 300ms following presentation of the target stimulus.