Operating on spiritual side of your individuality can bring you to the great heights, and drop you off if you neglect your spiritual identity. You are always looking for an opportunity to investigate the unknown, to use and show your mental abilities, to find the purpose and meaning of life. You want to grow wise and to understand people and things.
Typically, PCR consists of usually 20-40 repeated temperature changes, called cycles, where each cycles usually contains three steps. It is necessary to perform many cycles because that way the end results will consist mainly of the desired DNA.
The annealing temperature (typically between 48-72Â°C) is related to the melting temperature (Tm) of the primers and must be determined for each primer pair used in PCR. During the extension step (typically 68-72Â°C) the polymerase extends the primer to form a nascent DNA strand.