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This supports the idea that in embryos from wild-type mothers, dpp H~- mutations cause the transformation of dorsal into ven-tral epidermis. If tissue determined to become dorsal epidermis dies in the dpp rim-null embryos, then one should not see ventral or lateral tissue formed in the double-mutant combination.
Ribosomes engage the ER membrane through interaction of the signal recognition particle, SRP in the ribosome with the SRP receptor in the ER membrane. As the protein is synthesized the signal sequence is passed through the ER membrane into the lumen of the ER. After sufficient synthesis the signal peptide is removed by the action of signal peptidase. Synthesis will continue and if the protein is secreted it will end up completely in the lumen of the ER. If the protein is membrane associated a stop transfer motif in the protein will stop the transfer of the protein through the ER membrane. This will become the membrane spanning domain of the protein.