Operating on spiritual side of your individuality can bring you to the great heights, and drop you off if you neglect your spiritual identity. You are always looking for an opportunity to investigate the unknown, to use and show your mental abilities, to find the purpose and meaning of life. You want to grow wise and to understand people and things.
Typically, PCR consists of usually 20-40 repeated temperature changes, called cycles, where each cycles usually contains three steps. It is necessary to perform many cycles because that way the end results will consist mainly of the desired DNA.
Best Answer: Melting temp is when DNA strands separate, annealing temp is the temp where complimentary strands recombine (or anneal).The importance is this: In PCR, the temperature is cylced high to low, and a DNA polymerase that is heat-resistant is used so that it is not denatured at higher temps.f you don't reduce it to that lower temperature, the primers can never hydrogen bond with the DNA and cause extension. and this annealing temperature is CRUCIAL for the PCR to work.