Operating on spiritual side of your individuality can bring you to the great heights, and drop you off if you neglect your spiritual identity. You are always looking for an opportunity to investigate the unknown, to use and show your mental abilities, to find the purpose and meaning of life. You want to grow wise and to understand people and things.
Typically, PCR consists of usually 20-40 repeated temperature changes, called cycles, where each cycles usually contains three steps. It is necessary to perform many cycles because that way the end results will consist mainly of the desired DNA.
However, single-color methods appear adequate for accurate monitoring of temperature by fluorescence. Using fluorescence as a temperature monitor during PCR is dependent on data acquisition, which varies greatly from device to device, ranging from one data point per cycle to continuous acquisition.