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The total organic carbon content is subtracted from the total. carbon content to determine the total inorganic carbon content of a given sample. 1.0 INTRODUCTION. Total carbon (TC) includes both inorganic and inorganic sample constituents. Total. organic carbon (TOC) is determined by treating an aliquot of dried sample with sufficient. phosphoric acid (1:1) to remove inorganic carbon prior to instrument analysis. Percent.