Considerations. Each chocolate-covered espresso bean contains 5 milligrams of caffeine, a stimulant that elevates your heart rate and blood pressure. Overconsumption of caffeine can lead to nausea, vomiting, tremors, restlessness and difficulty sleeping.
A pH of about 7 is the optimum and as the pH moves further away from the optimum pH the enzyme activity starts to slow down. Every enzyme shows highest activity at a specific temperature which is called the optimum temperature. The rate of reaction above or below this temperature is slower. Increasing the temperature causes the frequency of collisions to increase as the molecules move faster when the heat is supplied.
The Effects of Temperature. Temperature has an effect on chlorine breakdown, but the effect is indirect. Many bacteria species grow better in warmer water, and when bacteria proliferate, free chlorine is used up more quickly as it kills them.