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Nutrient agar is a general-purpose, nutrient medium used for the cultivation of microbes. It is frequently used for isolation and purification of cultures. As we are using it here, it can also be used as a means for producing the bacterial lawns needed for antibiotic sensitivity tests.utrient agar consists of heat-stable digestive products of proteins (called peptones) and beef extract. Both of these provide amino acids, minerals, and other nutrients used by a wide variety of bacteria for growth.
Phenylethyl Alcohol Agar. Phenylethyl alcohol agar (PEA) is a selective medium used to cultivate Gram positive organisms. The active ingredient, phenylethyl alcohol, inhibits or markedly reduces growth of Gram negative organisms by interfering with DNA synthesis.PEA also prevents Proteus species from swarming across the surface of the agar.Staphylococcus aureus, a Gram positive organism, grows on this PEA plate while Serratia marcescens, a Gram negative organism, does not.EA also prevents Proteus species from swarming across the surface of the agar. Staphylococcus aureus, a Gram positive organism, grows on this PEA plate while Serratia marcescens, a Gram negative organism, does not.