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The mononuclear phagocyte system constitutes macrophage cells spread throughout the human body. The macrophages are long-lived cells that provide a major defense system against hostile elements through phagocytosis and subsequent intracellular destruction of the particle or cell.
Antinuclear antibodies (ANAs) are antibodies that react with a variety of proteins found in the nucleus of cells. In ANA testing, patient serum is placed on a tissue substrate fixed on a slide. Most laboratories use a HEp-2 cell line (a line of human skin cells) as the substrate for this test.