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Freebase(0.00 / 0 votes)Rate this definition: Levothyroxine. Levothyroxine, also L-thyroxine or T4, is a synthetic form of the thyroid hormone thyroxine, which is normally secreted by the follicular cells of the thyroid gland. It is also used to treat thyroid hormone deficiency, and occasionally to prevent the recurrence of thyroid cancer.
Not to be confused with Thyroxine-binding globulin, a carrier protein responsible for carrying the thyroid hormones in the blood. Thyroglobulin (Tg) is a 660 kDa, dimeric protein produced by the follicular cells of the thyroid and used entirely within the thyroid gland.g is secreted and stored in the follicular lumen. Via a reaction with the enzyme thyroperoxidase, iodine is covalently bound to tyrosine residues in thyroglobulin molecules, forming monoiodotyrosine (MIT) and diiodotyrosine (DIT). 1  Thyroxine is produced by combining two moieties of DIT.