Baking time 350 degrees 1 hour for every 1 1/2 pound. I use 2 pounds of chuck and 1 pound of sausage in my meatloaf and I always bake it at 350 for at least 3 hours but like I recommend cover with foil until the last 15 mins.aking time 350 degrees 1 hour for every 1 1/2 pound. I use 2 pounds of chuck and 1 pound of sausage in my meatloaf and I always bake it at 350 for at least 3 hours but like I recommend cover with foil until the last 15 mins.
Quick Answer. Ethanol is used in DNA extraction to force the DNA to precipitate in a solution. In order to collect a DNA sample, cells are broken down through agitation, then mixed with water, salt and ethanol to create an aqueous solution.
In this case, go back to the first step and add less water. You need an opaque cell âsoupâ for good yields. The amount of DNA you will get will depend on the ratio of DNA to cell volume rather than the number of chromosomes in your material. Plant seeds (such as peas) contain a high proportion of DNA. 3 The salt added helps the DNA to precipitate (as it clumps together) when it meets the ethanol phase. 4 It is important to allow time for each step to complete. The detergent must sit for at least 5 minutes to disrupt the cell membranes and nuclear membranes.