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During prophase, the nucleus disappears, spindle fibers form, and DNA condenses into chromosomes (sister chromatids). During metaphase, the sister chromatids align along the equator of the cell by attaching their centromeres to the spindle fibers. During anaphase, sister chromatids are separated at the centromere and are pulled towards opposite poles of the cell by the mitotic spindle. During telophase, chromosomes arrive at opposite poles and unwind into thin strands of DNA, the spindle fibers disappear, and the nuclear membrane reappears.
Anaphase is the third of four phases in mitosis. The four phases are: Prophase, Metaphase, Anaphase, and Telophase. In prophase, chromosomes migrate toward the cell center. In metaphase, chromosomes align along the center plane of the cell known as the metaphase plate. In anaphase, the duplicated paired chromosomes, known as sister chromatids, separate and begin moving toward opposite poles of the cell.