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Apoptosis is not the primary pathway of cell death after a freeze-thaw cycle, but its induction occurs in a significant part of mussel larval cells (up to 24%). Unlike sea urchin cells , we found that 5% dymethyl sulfoxide without any additives provided the maximum level of cell survival according to the MTT assay, trypan blue exclusion test, and DAPI staining to estimate cell viability.
It is not uncommon for those embryos that do survive freezing and thawing to lose a cell or two. Ideally the embryos should continue to divide between thawing and transfer. As embryo transfer is involved in using frozen embryos, the same risks apply. 1  Embryo transfer.he embryo storage process. Follow the life cycle of eggs for freezing and storage-from when they are first stored to their eventual use. Find out about storage time limits and how your eggs may eventually be used.