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Apoptosis is not the primary pathway of cell death after a freeze-thaw cycle, but its induction occurs in a significant part of mussel larval cells (up to 24%). Unlike sea urchin cells , we found that 5% dymethyl sulfoxide without any additives provided the maximum level of cell survival according to the MTT assay, trypan blue exclusion test, and DAPI staining to estimate cell viability.
1 frostbite, or tissue death, which is the most common complication that occurs when body tissues freeze. 2  chilblains, or nerve and blood vessel damage. 3  gangrene, or tissue destruction.  trench foot, which is nerve and blood vessel destruction from water immersion.