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Apoptosis is not the primary pathway of cell death after a freeze-thaw cycle, but its induction occurs in a significant part of mussel larval cells (up to 24%). Unlike sea urchin cells , we found that 5% dymethyl sulfoxide without any additives provided the maximum level of cell survival according to the MTT assay, trypan blue exclusion test, and DAPI staining to estimate cell viability.
Mechanisms of cell death in ischemia/reperfusion (I/R). I/R-induced necrosis generally occurs as a result of dysfunctional ion transport mechanisms, which causes cells to swell and eventually burst, effects that are exacerbated by plasma membrane damage.