A free particle (photon, electron etc) is not restricted to discrete energy levels. Its wavelength and therefore energy can take any value. A plane wave, with any wavelength you choose, can satisfy the Schrodinger equation for a single free particle. A free particle does not have to have a single precise energy. For example, a wave packet (a quantum wave that moves along like a fizzy ball) in free space has a range of energies. A particle that is confined will have discrete energy levels. For example, an electron in orbit around a nucleus. So quantum mechanics does not always mean that energy or any other property is quantized.
Cell Passage. Cell passaging or splitting is a technique that enables an individual to keep cells alive and growing under cultured conditions for extended periods of time.Cells should be passed when they are 90%-100% confluent. Ethanol squirt bottle and kimwipes.ell passaging or splitting is a technique that enables an individual to keep cells alive and growing under cultured conditions for extended periods of time. Cells should be passed when they are 90%-100% confluent. Ethanol squirt bottle and kimwipes.
So, most labs subculture their cells into a new vessel. This subculture is also known as a âpassage.â A passage number is the number of times a cell culture has been subcultured, and knowing the passage number can make or break an experiment.All cell cultures start somewhere; this âsomewhereâ is the reference strain, or reference culture.n a large, busy lab, itâs entirely possible to lose track of how many passages your cell culture has gone through. Keep a record, both on the vessel and in your computer or a notebook. The easier it is to check on passage number, the less likely youâll be working with altered cells.