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Apoptosis is not the primary pathway of cell death after a freeze-thaw cycle, but its induction occurs in a significant part of mussel larval cells (up to 24%). Unlike sea urchin cells , we found that 5% dymethyl sulfoxide without any additives provided the maximum level of cell survival according to the MTT assay, trypan blue exclusion test, and DAPI staining to estimate cell viability.
ICSI sperm injection into oocyte. Human oocyte cryopreservation (egg freezing) is a process in which a womanâs eggs (oocytes) are extracted, frozen and stored. Later, when she is ready to become pregnant, the eggs can be thawed, fertilized, and transferred to the uterus as embryos.he cost of the egg freezing procedure (without embryo transfer) is between $5,000 and $8,000. This does not include the fertility medications which can cost between $4,000 and $5,000. Egg storage can vary from $100 to several hundred dollars or more per year.