To make it clear what I'm talking about, I use a protocol like the following: 1  Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Meliodas is the captain of the Seven Deadly Sins and is the Dragon's Sin of Wrath. He is the owner of the renowned Boar Hat, and the main protagonist of the series. His Sacred Treasure is the demon-splitting sword, Lostvayne. Accelerator vs Meliodas.
Fairy Tail x The Seven Deadly SinsEdit. Upon arriving in Magnolia, Meliodas and his friends look around the sights of the town, admiring how it feels Christmasy. Subsequently, Meliodas asks Elizabeth if she wants anything for Christmas, to which she requests a talking cat. After stating that they already have a talking pig, the group encounter Happy, which surprises Meliodas and Elizabeth as they marvel the talking cat and neglect Hawk, who wanders off saddened.