Use DH5Î± cells in most cases. b. If want to cut at XbaI or other DAM-enzyme site, use SCS110 cells which are deficient in Dam and Dcm methylases. 2) Turn on water bath to 42 Î¿ C. 3) Put competent cells in a 1.5 ml tube (Eppendorf or similar). For transforming a DNA construct, use 50 ul of competent cells. For transforming a ligation, use 100 ul of competent cells. You may need more or less cells, depending how competent they are. 4) Keep tubes on ice.
Melinda Gates. Melinda Ann Gates (nÃ©e French; born August 15, 1964), DBE is an American philanthropist, former Microsoft employee, and wife of Microsoft founder Bill Gates. She is co-founder of the Bill & Melinda Gates Foundation. She worked at Microsoft, where she was project manager for Microsoft Bob, Encarta and Expedia.
The Bill and Melinda Gates Foundation has donated billions of dollars to causes all over the world, making significant differences in the lives of children and adults. This level of giving back illustrates Gatesâ character and focus on social responsibility as a global corporate citizen.