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Apoptosis is not the primary pathway of cell death after a freeze-thaw cycle, but its induction occurs in a significant part of mussel larval cells (up to 24%). Unlike sea urchin cells , we found that 5% dymethyl sulfoxide without any additives provided the maximum level of cell survival according to the MTT assay, trypan blue exclusion test, and DAPI staining to estimate cell viability.
Freeze-and-thaw-disrupted tumour cells impair the responsiveness of DC to TLR stimulation Eur. J. Immunol. 2008. 38: 2740â2750 Cancer immunotherapy aims at inducing immune responses against tumour associated antigens that mediate the eradication of tumour cells.