To make it clear what I'm talking about, I use a protocol like the following: 1  Take cells out of -80C and thaw on ice for 5 min. 2  Add 1 ul (~500 ng) plasmid DNA to 50 ul cells, mix gently with pipette tip. 3  Leave on ice for 30 min. 4  Put in 42C water bath for 45 sec.
Meliodas is the captain of the Seven Deadly Sins and is the Dragon's Sin of Wrath. He is the owner of the renowned Boar Hat, and the main protagonist of the series. His Sacred Treasure is the demon-splitting sword, Lostvayne. Accelerator vs Meliodas.
He also has an extensive knowledge of the world due to being well over 3000 years old. Longevity: Through unknown means Meliodas has a mysterious lifespan, being over 3000 years old and still having the appearance of a child.It was stated by the 10 Commandments that the lifespan of a demon is 1000 years and that if they hadn't been sealed for 3000 years they would have died 3 times already.Enhanced Durability: Meliodas has impressive durability, being able to withstand massive attacks and punches with little damage.t was stated by the 10 Commandments that the lifespan of a demon is 1000 years and that if they hadn't been sealed for 3000 years they would have died 3 times already. Enhanced Durability: Meliodas has impressive durability, being able to withstand massive attacks and punches with little damage.