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Apoptosis is not the primary pathway of cell death after a freeze-thaw cycle, but its induction occurs in a significant part of mussel larval cells (up to 24%). Unlike sea urchin cells , we found that 5% dymethyl sulfoxide without any additives provided the maximum level of cell survival according to the MTT assay, trypan blue exclusion test, and DAPI staining to estimate cell viability.
Human oocyte cryopreservation (egg freezing) is a process in which a womanâs eggs (oocytes) are extracted, frozen and stored.Later, when she is ready to become pregnant, the eggs can be thawed, fertilized, and transferred to the uterus as embryos.ith the first cryopreservation of sperm in 1953 and of embryos thirty years later, these techniques have become routine. Dr Christopher Chen of Singapore reported the worldâs first pregnancy in 1986 using previously frozen oocytes.