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Apoptosis is not the primary pathway of cell death after a freeze-thaw cycle, but its induction occurs in a significant part of mussel larval cells (up to 24%). Unlike sea urchin cells , we found that 5% dymethyl sulfoxide without any additives provided the maximum level of cell survival according to the MTT assay, trypan blue exclusion test, and DAPI staining to estimate cell viability.
A freeze occurs when temperatures drop below the freezing point of water (32Â° F or 0Â° C). When the water inside a plant freezes, it can cause the plant cells to burst, resulting in irreparable damage. Tropical and frost-tender plants: Cannot survive freezing temperatures so they only grow naturally in warmer climates.