Infrequent complications associated with ovarian cysts include: Ovarian torsion. Cysts that enlarge can cause the ovary to move, increasing the chance of painful twisting of your ovary (ovarian torsion). Symptoms can include an abrupt onset of severe pelvic pain, nausea and vomiting. Ovarian torsion can also decrease or stop blood flow to the ovaries.
Urease is a hydrolytic enzyme that attacks the nitrogen and carbon bond in amide compounds such as urea and forms the alkaline end product ammonia. The presence of urease is detectable when the organisms are grown in a urea broth medium containing the pH indicator phenol red. As the substrate urea is split into its products, the presence of ammonia creates an alkaline environment that causes the phenol red to turn to deep pink.
If the urea in the broth is degraded and ammonia is produced, an alkaline environment is created, and the media turns pink. Many enterics can hydrolyze urea; however, only a few can degrade urea rapidly. These are known as ârapid urease-positiveâ organisms. Members of the genus Proteus are included among these organisms. Urea broth is formulated to test for rapid urease-positive organisms. The restrictive amount of nutrients coupled with the use of pH buffers prevent all but rapid urease-positive organisms from producing enough ammonia to turn the phenol red pink. In our lab, we only use the urease broth, not the agar.