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The Fractionator Principle. The fractionator principle is one of the important concepts used in stereology. The importance of this principle cannot be overlooked. It appears in many different places. Although it looks different depending on how it is used, the principle is the same.
Differential centrifugation. Fractionation is a separation process in which a certain quantity of a mixture (gas, solid, liquid, suspension or isotope) is divided during a phase transition, into a number of smaller quantities (fractions) in which the composition varies according to a gradient.ractionation makes it possible to isolate more than two components in a mixture in a single run. This property sets it apart from other separation techniques.