Use DH5Î± cells in most cases. b. If want to cut at XbaI or other DAM-enzyme site, use SCS110 cells which are deficient in Dam and Dcm methylases. 2) Turn on water bath to 42 Î¿ C. 3) Put competent cells in a 1.5 ml tube (Eppendorf or similar). For transforming a DNA construct, use 50 ul of competent cells. For transforming a ligation, use 100 ul of competent cells. You may need more or less cells, depending how competent they are. 4) Keep tubes on ice.
Melinda Gates. Melinda Ann Gates (nÃ©e French; born August 15, 1964), DBE is an American philanthropist, former Microsoft employee, and wife of Microsoft founder Bill Gates. She is co-founder of the Bill & Melinda Gates Foundation. She worked at Microsoft, where she was project manager for Microsoft Bob, Encarta and Expedia.
Bill Gates was the top giver last year, according to the Philanthropy 50, assembled by the Chronicle of Philanthropy. Gates and his wife, Melinda, made a gift of $1.5 billion in Microsoft stock to the Bill and Melinda Gates Foundation. Emmanuel Dunand | AFP | Getty Images.