The incubation period is the time between when a disease enters your system (through the air, bodily contact or a cut in your skin for instance) and the time when you actually â¦ show symptoms of the disease. + 8 others found this useful.Deanna Stewart.he incubation period is the time between when a disease enters your system (through the air, bodily contact or a cut in your skin for instance) and the time when you actually â¦ show symptoms of the disease. + 8 others found this useful. Deanna Stewart.
DNA isolation is a process of purification of DNA from sample using a combination of physical and chemical methods.The first isolation of DNA was done in 1869 by Friedrich Miescher. Currently it is a routine procedure in molecular biology or forensic analyses. Breaking the cells open, commonly referred to as cell disruption or cell lysis, to expose the DNA within. 2  This is commonly achieved by chemical and physical methods-blending, grinding or sonicating the sample. 3  Removing membrane lipids by adding a detergent or surfactants which also serves in cell lysis.
DNA concentration can be determined measuring the intensity of absorbance of the solution at the 600 nm with a spectrophotometer and comparing to a standard curve of known DNA concentrations. Measuring the intensity of absorbance of the DNA solution at wavelengths 260 nm and 280 nm is used as a measure of DNA purity. DNA purification from detergents, proteins, salts and reagents used during cell lysis step. 2  The most commonly used procedures are: 3  Ethanol precipitation usually by ice-cold ethanol or isopropanol. 4  Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet upon centrifugation.