Edible anurans have to rely on more than just poison glands which tend to work too late (i.e. after the predator has it in his mouth!) Related articles: How to Rid Your Garden of Toads-tips for dog owners (aside from training the dog of course!)
Manevalâs staining method is a negative staining method. To stain a capsule of a cell by Manevalâs staining method. 1  1 % Congo red solution.  Manevalâs stain.
The tissue parts that specifically stain by this dye become blue to bluish-green after staining and are called Alcianophilic (akin to  eosinophilic  or  sudanophilic ). Alcian blue staining can be combined with H&E staining, PAS staining and van Gieson staining methods.Alcian blue can be used to quantitate acidic glycans both in microspectrophotometric quantitation in solution or for staining glycoproteins in polyacrylamide gels or on western blots.or many of these targets it is one of the most widely used cationic dyes for both light and electron microscopy. Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections.