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Apoptosis is not the primary pathway of cell death after a freeze-thaw cycle, but its induction occurs in a significant part of mussel larval cells (up to 24%). Unlike sea urchin cells , we found that 5% dymethyl sulfoxide without any additives provided the maximum level of cell survival according to the MTT assay, trypan blue exclusion test, and DAPI staining to estimate cell viability.
Some sperm do not survive or are damaged during freezing. This means that after freezing there may be a reduction in quality. Some frozen sperm samples that are of poor quality can only be used for Intra-cytoplasmic sperm injection (ICSI). Success rates are averaged over DI cycles and all IVF cycles using donor sperm.