Use DH5Î± cells in most cases. b. If want to cut at XbaI or other DAM-enzyme site, use SCS110 cells which are deficient in Dam and Dcm methylases. 2) Turn on water bath to 42 Î¿ C. 3) Put competent cells in a 1.5 ml tube (Eppendorf or similar). For transforming a DNA construct, use 50 ul of competent cells. For transforming a ligation, use 100 ul of competent cells. You may need more or less cells, depending how competent they are. 4) Keep tubes on ice.
Melinda Gates. Melinda Ann Gates (nÃ©e French; born August 15, 1964), DBE is an American philanthropist, former Microsoft employee, and wife of Microsoft founder Bill Gates. She is co-founder of the Bill & Melinda Gates Foundation. She worked at Microsoft, where she was project manager for Microsoft Bob, Encarta and Expedia.
Bill Gates, chairman of Microsoft and co-chair of the Bill, Melinda Gates Foundation, listens during the 2013 Fiscal Summit at Mellon Auditorium on May 7 in Washington.