A free particle (photon, electron etc) is not restricted to discrete energy levels. Its wavelength and therefore energy can take any value. A plane wave, with any wavelength you choose, can satisfy the Schrodinger equation for a single free particle. A free particle does not have to have a single precise energy. For example, a wave packet (a quantum wave that moves along like a fizzy ball) in free space has a range of energies. A particle that is confined will have discrete energy levels. For example, an electron in orbit around a nucleus. So quantum mechanics does not always mean that energy or any other property is quantized.
Cell Passage. Cell passaging or splitting is a technique that enables an individual to keep cells alive and growing under cultured conditions for extended periods of time.Cells should be passed when they are 90%-100% confluent. Ethanol squirt bottle and kimwipes.ell passaging or splitting is a technique that enables an individual to keep cells alive and growing under cultured conditions for extended periods of time. Cells should be passed when they are 90%-100% confluent. Ethanol squirt bottle and kimwipes.
The assumption here is one of cells reaching (near) confluence and requiring passage into fresh media at lower cell density. For tumor samples, some passage may be necessary to reduce numbers of unwanted cells (blood, fibroblast, etc.) that typically come along for ride when tissue is extracted.Yes, cells could be different after too much growth-especially tumor and tumor-derived cells.ADD COMMENT â¢ link written 5.0 years ago by Larry_Parnell â¦ 15k 1 Also AFAIK, this is the correct definition of passage. little off-topic, but hopefully someone will be able to help. I don't understand what low-passage means in this context. Example usage: 34 primary tumours and eight tumour-derived low passage cell lines were analyzed.