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Apoptosis is not the primary pathway of cell death after a freeze-thaw cycle, but its induction occurs in a significant part of mussel larval cells (up to 24%). Unlike sea urchin cells , we found that 5% dymethyl sulfoxide without any additives provided the maximum level of cell survival according to the MTT assay, trypan blue exclusion test, and DAPI staining to estimate cell viability.
Effect of oxaliplatin on the cell cycle in esophageal cancer cell lines. After oxaliplatin treatment, both cell lines showed an accumulation of cells in S phase, followed by G2/M-phase arrest (Fig. 2a). Later, TE3 cells started to accumulate at sub-G1 phase, indicating that cells were undergoing apoptosis.