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Apoptosis is not the primary pathway of cell death after a freeze-thaw cycle, but its induction occurs in a significant part of mussel larval cells (up to 24%). Unlike sea urchin cells , we found that 5% dymethyl sulfoxide without any additives provided the maximum level of cell survival according to the MTT assay, trypan blue exclusion test, and DAPI staining to estimate cell viability.
The most common types of reversible cell injury are manifested by accumulation of fluid (cellular swelling) and of fat (fatty change). Irreversibly injured cells die and have altered morphology. These morphologic patterns are recognized as necrosis or apoptosis.he syllabus for Cell Injury and Cell Death covers the material to be presented at the lectures on this topic (Jan. 3-5). The textbook reading for these lectures is Chapter 1 of Robbins and Cotran, 7th edition.