Preparation of MacConkey Agar. 1  Suspend the measured amount of powder (See in the agar bottle and generally 50 gram) in 1 L of purified water and mix thoroughly. 2  Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3  Autoclave at 121Â°C for 15 minutes.
All USDA loans (purchase and refinance) require a one-time Guarantee Fee that is added to the borrowers loan. The current amount of the USDA guarantee fee is 2%. Example â if a borrower was purchasing a home 100% financing for $150,000, the adjusted loan amount with the 2% USDA guarantee fee would be $153,000. $3,000 added to the base loan amount. In addition to this one time Guarantee Fee, USDA loans also require a annual fee that is added to the monthly mortgage payment. Basically the âannual feeâ is monthly mortgage insurance â or more commonly known as âPMIâ Again, like the above it can have many different names. The current amount of this fee is .5%.
The upfront PMI that USDA charges on loans (called Upfront Mortgage Insurance or Guarantee Fee) can be included in the loan. This means that if you are purchasing a $100,000 home in Raleigh, the loan will be $102,000; OR, you can pay the 2% out of your pocketâ¦ or see if you can get the Seller to pay it.o calculate that â you take your total loan amount, so the amount you are paying for the home, letâs say itâs $200,000, plus the 2% for the Upfront USDA PMI which would be $4000. So, in this case, $204,000 times .4%. That equals $816, which is the annual USDA PMI fee.