The exact causes of chronic kidney failure are unknown, although the debilitating loss of healthy kidney tissue may be a natural endpoint for several readily recognizable conditions, including advanced dental disease and a variety of kidney infections, inflammations and obstructions.
Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA. Okay, the person above does not understand Chemistry nor the English language. The sodium chloride, NaCl, is a polar molecule, which would help separate an emulsion of two layers in an acid-base extraction. Since NaCl is polar, it would be easily dissolved into the solution and tie up the different layer molecules.
1 Breaking the cells open, commonly referred to as cell disruption or cell lysis, to expose the DNA within. 2  This is commonly achieved by chemical and physical methods-blending, grinding or sonicating the sample. 3  Removing membrane lipids by adding a detergent or surfactants which also serves in cell lysis. DNA purification from detergents, proteins, salts and reagents used during cell lysis step. 2  The most commonly used procedures are: 3  Ethanol precipitation usually by ice-cold ethanol or isopropanol. 4  Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet upon centrifugation.