district court ruled in favor of an issuer of traveler's checks after the Kentucky legislature attempted to shorten the dormancy period for un-cashed traveler's checks from 15 to 7 years (American Express Travel Related Servs.
The first step in a PCR cycle is the denaturation step. This is the PCR step in which the hydrogen bonds holding the complementary strands of DNA together are broken. The second step in a PCR cycle is the annealing step. The annealing step is the PCR step in which the primers anneal, or attach, to the DNA template. The third step in a PCR cycle is the extension step. The extension step, also referred to as the elongation step, is the PCR step in which Taq polymerase adds nucleotides to the annealed primer.
PCR works by repeated cycles of strand separation, annealing of primers, and extension of the primed strands. Simply put, the target DNA is replicated in vitro, many, many times, to obtain a large amount of the DNA that lies between the two primer regions.n PCR, the piece of DNA we want is copied many times over in the test tube. In each cycle of PCR, the following steps are done: 1. The original DNA (template) is heated to separate the strands, to make them available for binding with the primers. This is called denaturing.