district court ruled in favor of an issuer of traveler's checks after the Kentucky legislature attempted to shorten the dormancy period for un-cashed traveler's checks from 15 to 7 years (American Express Travel Related Servs.
The first step in a PCR cycle is the denaturation step. This is the PCR step in which the hydrogen bonds holding the complementary strands of DNA together are broken. The second step in a PCR cycle is the annealing step. The annealing step is the PCR step in which the primers anneal, or attach, to the DNA template. The third step in a PCR cycle is the extension step. The extension step, also referred to as the elongation step, is the PCR step in which Taq polymerase adds nucleotides to the annealed primer.
DNA synthesis cycle. 1  2 After the reacting solutions are prepared, the PCR cycle is started. The first phase involves the denaturation of DNA. 2  3 With the DNA split into separate strands, the temperature is lowered to 122-149Â°F (50-65Â°C). This is known as the primer annealing step and lasts for about two minutes.