district court ruled in favor of an issuer of traveler's checks after the Kentucky legislature attempted to shorten the dormancy period for un-cashed traveler's checks from 15 to 7 years (American Express Travel Related Servs.
The first step in a PCR cycle is the denaturation step. This is the PCR step in which the hydrogen bonds holding the complementary strands of DNA together are broken. The second step in a PCR cycle is the annealing step. The annealing step is the PCR step in which the primers anneal, or attach, to the DNA template. The third step in a PCR cycle is the extension step. The extension step, also referred to as the elongation step, is the PCR step in which Taq polymerase adds nucleotides to the annealed primer.
At its optimum temperature, the DNA polymerase will polymerize a thousand bases per minute. After the last PCR cycle, a single step of final elongation is required to ensure that any remaining single-stranded DNA is fully extended. This step occurs at 70-74 degrees Celsius for 5-15 minutes.