The exact causes of chronic kidney failure are unknown, although the debilitating loss of healthy kidney tissue may be a natural endpoint for several readily recognizable conditions, including advanced dental disease and a variety of kidney infections, inflammations and obstructions.
Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA. Okay, the person above does not understand Chemistry nor the English language. The sodium chloride, NaCl, is a polar molecule, which would help separate an emulsion of two layers in an acid-base extraction. Since NaCl is polar, it would be easily dissolved into the solution and tie up the different layer molecules.
Mechanism of action: Cyclophosphamide is an inactive drug. With the help of cytochrome P-450 oxidase system in the liver, the inactive drug is converted into phosphoramide mustard and acrolein which are very active compounds. Phosphoramide mustard has an ability to introduces alkyl radicals into DNA strands with interferes DNA replication by forming DNA cross-linkage. Cross-linked cancer cell DNA is unable to complete normal cell division. Thus, it stops cancer cells from growing, causing them to die.