district court ruled in favor of an issuer of traveler's checks after the Kentucky legislature attempted to shorten the dormancy period for un-cashed traveler's checks from 15 to 7 years (American Express Travel Related Servs.
The first step in a PCR cycle is the denaturation step. This is the PCR step in which the hydrogen bonds holding the complementary strands of DNA together are broken. The second step in a PCR cycle is the annealing step. The annealing step is the PCR step in which the primers anneal, or attach, to the DNA template. The third step in a PCR cycle is the extension step. The extension step, also referred to as the elongation step, is the PCR step in which Taq polymerase adds nucleotides to the annealed primer.
During the first step of PCR, called denaturation, the tube containing the sample DNA is heated to more than 90 degrees Celsius (194 degrees Fahrenheit), which separates the double-stranded DNA into two separate strands.