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Supplement. The operon acts as a unit of transcription, and functions in a coordinated manner to produce by transcription a single mRNA molecule which codes for more than one protein with related metabolic functions. The first described operon is the lac operon.Related terms: 1  Lac operon. 2  His operon.peron. Definition. noun, plural: operons. A group of genes or a segment of DNA that functions as a single transcription unit. It is comprised of an operator, a promoter, and one or more structural genes that are transcribed into one polycistronic mRNA.