Preparation of MacConkey Agar. 1  Suspend the measured amount of powder (See in the agar bottle and generally 50 gram) in 1 L of purified water and mix thoroughly. 2  Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. 3  Autoclave at 121Â°C for 15 minutes.
All USDA loans (purchase and refinance) require a one-time Guarantee Fee that is added to the borrowers loan. The current amount of the USDA guarantee fee is 2%. Example â if a borrower was purchasing a home 100% financing for $150,000, the adjusted loan amount with the 2% USDA guarantee fee would be $153,000. $3,000 added to the base loan amount. In addition to this one time Guarantee Fee, USDA loans also require a annual fee that is added to the monthly mortgage payment. Basically the âannual feeâ is monthly mortgage insurance â or more commonly known as âPMIâ Again, like the above it can have many different names. The current amount of this fee is .5%.
USDA Up front âGuarantee Feeâ (this is the upfront fee the USDA charges to participate in the Rural Housing program and is usually financed (rolled into) the borrower new loan). USDA Purchase & USDA refinance transactions: new fee effective 2015 â 2% (no change). An example of how the upfront fee is calculated.For a sales price of $150,000, the upfront fee would be 2% or $3000, and typically that would be added to the loan amount to make the final loan amount $153,000.he upfront USDA Guarantee Fee for all new Florida USDA 502 Guarantee purchase and refinance loans as of October 1 2014 â October 1 2015 will be as follows: