The exact causes of chronic kidney failure are unknown, although the debilitating loss of healthy kidney tissue may be a natural endpoint for several readily recognizable conditions, including advanced dental disease and a variety of kidney infections, inflammations and obstructions.
Sodium chloride was needed to ensure the proteins in the cell aren't separated from the rest of the solution with the DNA. Okay, the person above does not understand Chemistry nor the English language. The sodium chloride, NaCl, is a polar molecule, which would help separate an emulsion of two layers in an acid-base extraction. Since NaCl is polar, it would be easily dissolved into the solution and tie up the different layer molecules.
Best Answer: DNA extraction procedure: See number 8 re:chloroform 1.Treat 25 mg of tissue with Proteinase K by adding 20 ml of stock PK solution (20 mg/ml) and 180 ul water.Function: proteinase destroys protein. 2.Let sit at 55 degrees for at least 1 hour.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.