According to Deadline, A&E has chosen not to renew its hit western Longmire for a season 4. Yet the news outlet does say Warner Horizon, the studio behind the series, will indeed be shopping it immediately to other networks. In a statement, A&E didnât elaborate on their decision, simply stating:
Smashwords (Smashwords, Inc.), based in Los Gatos, California, is an ebook distribution platform founded by Mark Coker for independent authors and publishers.mashwords also uploaded books into KDP, Amazon .com's ebook publishing website, but until February 2014 no longer offered the service, due to a falling out with Amazon. As of February 2014, limited distribution to Amazon via Smashwords resumed.
This article is about Chromosomes derived from yeast DNA. For Yeast created from synthetic DNA, see Synthetic DNA. Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces cerevisiae, which is then ligated into a bacterial plasmid. YAC is built using an initial circular DNA plasmid, which is typically cut into a linear DNA molecule using restriction enzymes; DNA ligase is then used to ligate a DNA sequence or gene of interest into the linearized DNA, forming a single large, circular piece of DNA.