C. difficile infection requires documenting the presence of toxin in the stools, usually by testing for the gene that produces toxin B, using a method called PCR. It is very sensitive, so it should not be used to test solid stools since that is likely a carrier state.
Definition of vassal. 1  1 : a person under the protection of a feudal lord to whom he has vowed homage and fealty : a feudal tenant. 2  2 : one in a subservient or subordinate position.
Many vassals in the Middle Ages led the role of both vassal and lord. This meant that they were vassals to a lord, but also leased some of the land that they were granted. In fact, in the feudal system, a majority of nobility were probably in this situation.