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Summary Information: Valve Cusps Valve Cusps: Valve cusps are located in the valves of the heart and are flaps of tissue that open in one direction as blood is pumped through them. The valve cusps are made of thin fibrous tissue, covered with a smooth membrane (endocardium) and reinforced by connective tissue.
1 The mitral valve is located between the left atrium and the left ventricle. It is the only valve with two flaps, or cusps.  The tricuspid valve is located on the right side of the heart, between the right atrium and right ventricle. It is made up of three cusps, each a different size.