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ATP synthase is a ubiquitous enzyme that is located in the inner membranes of mitochondria, thylakoid membranes of chloroplasts, or the plasma membranes of bacteria.
Most of the ATP in cells is produced by the enzyme ATP synthase, which converts ADP and phosphate to ATP. ATP synthase is located in the membrane of cellular structures called mitochondria; in plant cells, the enzyme also is found in chloroplasts.