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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
Primase-The enzyme responsible for initiating synthesis of RNA primers on the lagging strand during DNA replication. Holoenzyme-A term used to describe a collection of different enzymes that work together in a given process such as DNA replication.arent strand-In DNA replication, refers to the pre-existing single strand of DNA that is copied into a new strand of DNA via complementary base pairing. Pyrophosphate-A two phosphate-containing molecule.