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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
The primary enzymes responsible for DNA replication are DNA polymerases. Understanding the activity and limitations of DNA polymerases help in making sense of why DNA replication occurs the way it does. Key points are: 1  Prokaryotic cells contain three different DNA polymerases.2  Each have slightly different activities.uring DNA replication a DNA double helix must unwind and separate so that DNA polymerase enzymes can use each single strand as a template for the synthesis of a new double strand. Strand separation is catalyzed by a Helicase enzyme.