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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
Following creation of RNA primers by primase on the lagging strand, DNA polymerase Î´ synthesizes Okazaki fragments. DNA polymerase Î´ also carries out a 3' to 5' exonuclease role, proofreading newly synthesized DNA strands during DNA replication.