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ATP synthase is a ubiquitous enzyme that is located in the inner membranes of mitochondria, thylakoid membranes of chloroplasts, or the plasma membranes of bacteria.
This process is related to osmosis, the diffusion of water across a membrane, which is why it is called chemiosmosis. ATP synthase is the enzyme that makes ATP by chemiosmosis. It allows protons to pass through the membrane and uses the free energy difference to phosphorylate adenosine diphosphate (ADP), making ATP. The generation of ATP by chemiosmosis occurs in mitochondria, chloroplasts as well as in most bacteria and archaea.