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Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. Importantly, S. aureus is generally coagulase-positive, meaning that coagulase negativity usually excludes S. aureus. However it is now known that not all S. aureus are coagulase-positive.
Coagulase. This simple test shows the organism's ability to produce the enzyme coagulase. In the laboratory the bacteria is added to rabbit plasma and incubated. The tests are read for the presence of a clot. Staphylococcus aureus is positive for coagulase while Staphylococcus epidermidis is negative.taphylococcus aureus is a large, smooth and slightly raised colony that usually produces beta-hemolysis. Many colonies also show a golden-yellow pigment on sheep blood agar. The colonies of Staphylococcus epidermidis are smaller, gray-white and non-hemolytic.