The MacBook Pro follows, with its prices going from $2,199 down to $1,999 for the 15-inch version (and from $1,499 for the 13-inch version down to $1,299). That's a break from traditional Apple pricing, where prices would remain the same generation over generation, with updated components adding value.
Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid.
Add 3-4 additional GC's to get sufficient digestion of PCR product. you select the nearest matching restriction buffer in which one of the enzyme shows 100% activity and another enzyme at least shows 50 % activity in selected buffer. Add the double amount of enzyme showing 50% activity.