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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
In addition to these proteins, several other enzymes are involved in bacterial DNA replication. DNA Polymerase-DNA Polymerase I (Pol I) was the first enzyme discovered with polymerase activity, and it is the best characterized enzyme.roteins of DNA Replication. 1  DNA Helicases-These proteins bind to the double stranded DNA and stimulate the separation of the two strands. 2  DNA single-stranded binding proteins-These proteins bind to the DNA as a tetramer and stabilize the single-stranded structure that is generated by the action of the helicases.