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In eukaryotes, transcription factors (like most proteins) are transcribed in the nucleus but are then translated in the cell's cytoplasm. Many proteins that are active in the nucleus contain nuclear localization signals that direct them to the nucleus.hus, for a single transcription factor to initiate transcription, all of these other proteins must also be present, and the transcription factor must be in a state where it can bind to them if necessary. Cofactors are proteins that modulate the effects of transcription factors.
However, we are concerned mainly with the differences. Both transcription and translation are much more complex processes in Eukaryotes than in Prokaryotes (Well, the structure and functional capacities of Eukaryotic cells are much more complex and rich in almost every other aspect as compared to Prokaryotic cells). In Eukaryotic cells, transcription and transcription occur in separate compartments of the cell. Transcription takes place inside a membrane-bound compartment, the nucleus. At first, a pre-mRNA is synthesized by the action of RNA Polymerase.