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ATP synthase is a ubiquitous enzyme that is located in the inner membranes of mitochondria, thylakoid membranes of chloroplasts, or the plasma membranes of bacteria.
F1Fo ATP Synthase of mitochondria, chloroplasts, and bacteria is represented schematically at right. When the electrochemical H + gradient is favorable, F 1 F o catalyzes ATP synthesis coupled to spontaneous H+ flux toward the side of the membrane where F 1 protrudes. E.g., in mitochondria, the pH and electrical gradients drive H + transport from the intermembrane space to the matrix compartment.