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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
Several enzymes participate in DNA replication, one being helicase which unwinds the two nucleotide strands. Binding proteins stabilize the single stranded DNA and DNA polymer â¦ ase attach the free nucleotides to the growing strand.NA Polymerase III is responsible for adding new nucleotides to the strand being synthesised. Also involved in DNA replication are DNA Polymerase I which replaces primers wi â¦ th nucleotides, and DNA Ligase which joins fragments of DNA together. 1 person found this useful.