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Confidence votes 106. The role that enzymes plays in DNA replication is that the enzyme helicase breaks hydrogen bonds between the complementary bases, and the chains separate.
To synthesize the two chains of deoxyribonucleotides during DNA replication, the DNA polymerase enzymes involved are only able to join the phosphate group at the 5' carbon of a new nucleotide to the hydroxyl (OH) group of the 3' carbon of a nucleotide already in the chain. 5.