The MacBook Pro follows, with its prices going from $2,199 down to $1,999 for the 15-inch version (and from $1,499 for the 13-inch version down to $1,299). That's a break from traditional Apple pricing, where prices would remain the same generation over generation, with updated components adding value.
Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid.
Restriction enzymes (for example Restriction Endonuclease) are used to cut across the two strands of DNA and leave loose ends to which the cDNA can be attached. Special linking DNA sequences are added to connect the plasmid DNA with the human insulin cDNA.