The MacBook Pro follows, with its prices going from $2,199 down to $1,999 for the 15-inch version (and from $1,499 for the 13-inch version down to $1,299). That's a break from traditional Apple pricing, where prices would remain the same generation over generation, with updated components adding value.
Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid.
Restriction enzymes cut through both nucleotide strands, breaking the DNA into fragments, but they donât always do this in the same way. SmaI is an example of a restriction enzyme that cuts straight through the DNA strands, creating DNA fragments with a flat or blunt end. Other restriction enzymes, like EcoRI, cut through the DNA strands at nucleotides that are not exactly opposite each other. This creates DNA fragments with one nucleotide strand that overhangs at the end.