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Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. Importantly, S. aureus is generally coagulase-positive, meaning that coagulase negativity usually excludes S. aureus. However it is now known that not all S. aureus are coagulase-positive.
The Coagulase Test. The Coagulase Test is used to differentiate Staphylococcus aureus from other species of this genus. To perform the Slide Coagulase Test, place a drop of coagulase reagent (rabbit plasma) onto a clean microscope slide, and then add several colonies of the unknown Staphylococcus.Mix well.o perform the Slide Coagulase Test, place a drop of coagulase reagent (rabbit plasma) onto a clean microscope slide, and then add several colonies of the unknown Staphylococcus. Mix well.