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Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. Importantly, S. aureus is generally coagulase-positive, meaning that coagulase negativity usually excludes S. aureus. However it is now known that not all S. aureus are coagulase-positive.
Coagulase test is used to differentiate Staphylococcus aureus (positive) from Coagulase Negative Staphylococcus (CONS). Coagulase is an enzyme produced by S. aureus that converts (soluble) fibrinogen in plasma to (insoluble) fibrin.Staphylococcus aureus produces two forms of coagulase, bound and free.lide Coagulase Test Procedure. 1  Take a clean grease free and dry glass slide. 2  Place a drop of plasma. 3  Place a drop of distilled water or saline next to the drop of plasma as a negative control. 4  Emulsify a portion of the isolated colony being tested in each drop, and make a smooth milky suspension (not clumpy).