But there is a way for you to become a better putter from long distances. The first rule when you have a longer putt is to avoid the three putt. If you can become a better lag putter, you will certainly score better and yes, a few of those putts hit with the right speed on a good line, are going to drop.
Definition of shake culture. : a deep culture of agar or gelatin through which the inoculum is evenly distributed by shaking before the medium is solidified and which is used chiefly for the demonstration of anaerobic colonies.
If the bacteria on your LB agar plates are not fresh, you should streak your bacteria onto a new LB agar plate before growing in liquid culture. More aeration may help to increase the density of the culture. Normally cultures shake at 150 - 250 rpm, increase this to 350 - 400 rpm to obtain a higher cell density.fter incubation, check for growth, which is characterized by a cloudy haze in the media (see right). Note: Some protocols require bacteria to be in the log phase of growth. Check the instructions for your specific protocol and conduct an OD600 to measure the density of your culture if needed.