Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
DNA run off the gel. Perform electrophoresis until the bromophenol blue dye passes 2/3 (orange G, 4/5) of the gel. Refer to the table on p.441 for migration of tracking dyes in different gels. Make sure that the entire gel is immersed completely in the electrophoresis buffer during the run.