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Probably limited a small bit by structural strength, i.e. more acceleration means a stronger and heavier structure. For manned rockets, the G forces are even lower to accomodate the astronauts. So I'm assuming that even lower accelerations are only possible if you trade off fuel efficiency.
NASA had a definition in their 1965 dictionary of Technical Terms for Aerospace Use: g or G. An acceleration equal to the acceleration of gravity, 980.665 centimeter-second-squared, approximately 32.2 feet per second per second at sea level; used as a unit of stress measurement for bodies undergoing acceleration. See acceleration of gravity; gravity.