Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
The procedure goes like this: You will need two containers, one with the dye bath (prepare as usual), and one filled with rinse water, and gloves to protect your hands from being dyed. Heat the dye bath till warm but not so hot you cannot put your hand into it. You don't want to activate the dye just yet but the warm water helps the yarn to obsorb it since it is not pre wetted. EDIT: You want the dye to be active otherwise the dye will come out in the rinse water.