Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
1 Mix up your soda ash. 2  Soda ash, or sodium carbonate, is used to bond the dye to the cellulose in fiber. 3  Dissolve the ash in warm water and add it into the tub slowly (over the course of 15 minutes), being careful not to dump it directly onto the fabric (which could cause discoloration).