Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
1 Place the ball into the dye bath, holding it down till it sinks if nessecary.  Right away, take a five foot length of yarn (more or less to suit your needs), and while pinching it through your finger tips on one hand-draw it out of the dye bath, through the pinch, and let it fall into the rinse water.