Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Exercise 3: Simple Stains, Hanging Drop, and Wet-Mount Preparations 1. Define acidic and basic dyes. What is the purpose of each? Acidic dyes are anions, or negatively charged, that work with a base in the specimen to create a salt.