Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Procedure. 1  Put on gloves. You may be sharing dye samples and the agarose gel with another lab group. 2  Seal each end of the gel tray with laboratory tape. Place the plastic comb into the middle of the tray. 3  Carefully pour the agarose into the gel tray until it is approximately 1/3 of the way up the teeth of the comb.