Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
The difficulty with polyester dyeing is that it requires a lot of heat to get the dye into the fiber. Boiling water is not hot enough to do the job by itself, so a noxious-smelling carrier chemical must be added, for immersion dyeing, unless newer low-energy disperse dyes are used.