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Ethanol precipitation is a method used to purify and/or concentrate RNA, DNA and polysaccharides such as pectin and xyloglucan from aqueous solutions by adding ethanol as an antisolvent.Contents.thanol precipitation is a method used to purify and/or concentrate RNA, DNA and polysaccharides such as pectin and xyloglucan from aqueous solutions by adding ethanol as an antisolvent. Contents.
DNA falls out of solution in 35% isopropanol and 0.5 M salt. Using ethanol, the final concentration needs to be around 75% with 0.5 M salt. So for the typical precipitation protocol, isopropanol is added from between 0.7â1 volumes of sample and ethanol is added at 2-2.5 volumes of sample.