Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
In gel electrophoresis, samples of DNA or proteins are separated -- typically based on size -- by applying an electric field that causes them to migrate through a gel.nstructions. Check your lab notebook to determine which samples were loaded into which lanes. When you loaded the wells for your gel, you should have noted the identity of each lane/sample. Determine which lane contains the ladder of DNA standards.