Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Prepare the 5 egg dye solutions using the following measurements: 1  Vinegar: 2 teaspoons vinegar + 1 cup water. 2  Lemon juice: 2 teaspoons lemon juice + 1 cup water. 3  Cream of tarter: 1/4 teaspoon cream of tartar + 1 cup water (room temp water; stir well to dissolve) 4  Water-only: 1 cup water with nothing added acid a.