Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Setting up of Gel Apparatus and Separation of DNA Fragments. 1  Add loading dye to the DNA samples to be separated (Fig. 2). 2  Program the power supply to desired voltage (1-5V/cm between electrodes). 3  Add enough running buffer to cover the surface of the gel. 4  Attach the leads of the gel box to the power supply. 5  Remove the lid.