Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Gelcoat will need to be heavier in thickness for a couple of reasons. Foremost is that gelcoat doesn't level out and cure the same way that paint does. Even with additives to thin the viscosity, a certain degree of texture, (orange peel), will be present after cure and has to be sanded, sealed, and polished.. 10 mils gelcoat-little or no additives* & no surfacing wax. 2. 10 mils gelcoat-little or no additives* & no surfacing wax. 3. 10 mils gelcoat-with additives* & surfacing wax. Allow the gelcoat to harden for several hours between coats.