Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Use a dye made for protein (wool, silk, etc.) It should take the dye easily. If the rope is tightly spun, the dye might not reach the inner filament sections so easily. Some things (like pantyhose) are actually manufactured/knit white, then dyed before final assembly and packaging. Written Mar 7.