Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
The sulphuric acid has an unknown concentration. Then you will fill a BURETTE with sodium hydroxide (a base) that has a known concentration. Because all the reactants and products in this reaction are colourless, an. INDICATOR DYE is added to the sulphuric acid to let us know when all the acid present has been EXACTLY.