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In metaphase, sister chromatids align along the metaphase plate at right angles to the cell poles. In anaphase, sister chromatids separate and begin moving toward opposite ends of the cell. Once the paired sister chromatids separate from one another, each chromatid is considered a single-stranded, full chromosome. In telophase and cytokinesis, separated sister chromatids are divided into two separate daughter cells. Each separated chromatid is referred to as a daughter chromosome.
Sister Chromatids (formed during interphase) â These are joined together by a centromere. â¢ Specific DNA sequence. â¢ Bound to the centromere is the kinetochore. â¢ The kinetochore is a disc of proteins which connects the centromere to the spindle fibers.