Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
What does the loading buffer for agarose gel electrophoresis of nucleic acids contain and why? contains something dense (ex glycerol) to allow the sample to fall into the sample wells and also contains one or two tracking dyes which migrate in the gel and allow monitoring how far the electrophoresis has proceeded