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Effects of substituents on the acidity of phenols: Existence of electron attracting group, (for example-NO2, -X, - NR, -CN, -CHO, -COOH) on the benzene ring increases the acidity of phenol since it enables the ring to draw much more electrons from the phenoxy oxygen and so releasing easily the proton.
The broth in this contains Phenol Red, a pH indicator. A pH below 6.4 will turn the Phenol Red to Yellow. During fermentation, acids are produced that lower pH. You CAN have one result without the other, or both as the same result (Gas and Fermentation).