Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
The gel, still in its plastic tray, is inserted horizontally into the electrophoresis chamber and just covered with buffer. Samples containing DNA mixed with loading buffer are then pipeted into the sample wells, the lid and power leads are placed on the apparatus, and a current is applied.