Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Migration of. a molecule may also affected by the density of the gel and the type of buffer used. These properties of electrophoresis can be demonstrated using food coloring. When mixed with glycerin or sucrose, food dyes can be loaded into the wells of an. agarose gel and separated into their constituent pigments.