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Catalog No.S4186. Domiphen bromide is a quaternary ammonium antiseptic with actions as a cationic surfactant.In vitro. Recombinant 4-diphosphocytidyl-2-C-methyl-d-erythritol synthase (IspD) is sensitive to Domiphen Bromide (DMB), as observed through an IspD activity assay.The inhibitory effect of DMB on IspD is confirmed by both the newly-established high-throughput screening system and traditional enzyme kinetic assays.omiphen bromide is a quaternary ammonium antiseptic with actions as a cationic surfactant. In vitro. Recombinant 4-diphosphocytidyl-2-C-methyl-d-erythritol synthase (IspD) is sensitive to Domiphen Bromide (DMB), as observed through an IspD activity assay.
, adenovirus does not measurably precipitate at concentrations of domiphen bromide below 0.05%. Therefore, addition of domiphen bromide at levels between 0.03 and 0.05% will result in selective precipitation of DNA. For the particular stream properties used here, addition at 0.04% is suitable for all serotypes. first step in a purification process of the present invention is a host cell lysis step, which provides for maximum release of adenovirus particles from the cells as well as providing for an opportunity to potentially inactivate adventitious agents.