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Probably limited a small bit by structural strength, i.e. more acceleration means a stronger and heavier structure. For manned rockets, the G forces are even lower to accomodate the astronauts. So I'm assuming that even lower accelerations are only possible if you trade off fuel efficiency.
negative G-forces. When the roller coaster is going over the top of a hill, the riders will feel lighter in weight and they may even experience weightlessness. The decreased amount of your bodies weight due to a motion that is opposite of gravity is known as negative G-force.