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In metaphase, sister chromatids align along the metaphase plate at right angles to the cell poles. In anaphase, sister chromatids separate and begin moving toward opposite ends of the cell. Once the paired sister chromatids separate from one another, each chromatid is considered a single-stranded, full chromosome. In telophase and cytokinesis, separated sister chromatids are divided into two separate daughter cells. Each separated chromatid is referred to as a daughter chromosome.
During mitosis, which occurs after chromosomes are duplicated during S phase, two sister chromatids are held together, each with its own kinetochore, which face in opposing directions and attach to opposite poles of the mitotic spindle.