Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
2. For best viewing, the gel should be placed on a light source (a light box or overhead projector) or on a white background (a piece of unlined paper). 3. The gel results should be recorded within a couple of hours after running the gel. After this time period, the dye pigment bands will start to diffuse into the gel and will be difficult