Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
Preparing the DNA for electrophoresis. 1  A dye is added to the sample of DNA prior to electrophoresis to increase the viscosity of the sample which will prevent it from floating out of the wells and so that the migration of the sample through the gel can be seen.