Dr. Grace Dzido, MD is an internal medicine doctor who practices in Roanoke, VA. She is 54 years old and has been practicing for 19 years.
The Loading Dye * Loading dye is a colored buffer mixed with the DNA prior to loading onto the gel. The loading dye contains a relatively high concentration of either glycerol or sucrose. This makes the solution more dense than the surrounding running buffer so that when a sample is pipetted over top of a well it sinks down into the well.
As a gel stain for DNA, Diamondâ¢ Nucleic Acid Dye performed similarly to SYBRÂ® Gold Nucleic Acid Gel Stain and was more sensitive than SYBRÂ® Safe DNA Gel Stain (Figure 1, Panel A). In precast gels, Diamondâ¢ Nucleic Acid Dye and SYBRÂ® Gold Nucleic Acid Gel Stain stained all DNA bands, but due to exposure effects, only concentrations in the middle dilution range were effectively visualized (Figure 1, Panel B).