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Probably limited a small bit by structural strength, i.e. more acceleration means a stronger and heavier structure. For manned rockets, the G forces are even lower to accomodate the astronauts. So I'm assuming that even lower accelerations are only possible if you trade off fuel efficiency.
The g-force acceleration (save for certain electromagnetic force influences) is the cause of an object's acceleration in relation to free-fall. The g-force acceleration experienced by an object is due to the vector sum of all non-gravitational and non-electromagnetic forces acting on an object's freedom to move. In practice, as noted, these are surface-contact forces between objects.