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Probably limited a small bit by structural strength, i.e. more acceleration means a stronger and heavier structure. For manned rockets, the G forces are even lower to accomodate the astronauts. So I'm assuming that even lower accelerations are only possible if you trade off fuel efficiency.
Here is a list of some of the known issues of zero g: 1  bone loss, lose 1 or 2% of your bone mass every month in load bearing parts of your skeleton such as feet. 2  eye problems (many astronauts have short term issues after their flight, and there's been one case of irreversible damage to sight as a result of zero g),