A number of non-infectious causes can result in diarrhea. These include lactose intolerance, irritable bowel syndrome, non-celiac gluten sensitivity, celiac disease, inflammatory bowel disease, hyperthyroidism, and a number of medications. In most cases, stool cultures to confirm the exact cause are not required.
Most dogs are first bred between the 10th and 14th day after the onset of proestrus. As long as the bitch will accept the male, mating every other day for a total of two or three matings is generally considered sufficient. However, signs of proestrus are not obvious in some bitches.
Your dog will experience a heat cycle before she is able to get pregnant. Veterinarians suggest that you do not breed her during her first heat period unless it happens after she is 1 year old. Any earlier would stunt the growth of your young female.