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In metaphase, sister chromatids align along the metaphase plate at right angles to the cell poles. In anaphase, sister chromatids separate and begin moving toward opposite ends of the cell. Once the paired sister chromatids separate from one another, each chromatid is considered a single-stranded, full chromosome. In telophase and cytokinesis, separated sister chromatids are divided into two separate daughter cells. Each separated chromatid is referred to as a daughter chromosome.
A chromatid is formed during meiosis and is one of two identical DNA strands formed after DNA synthesis. Sister chromatids are from the same chromosome, and they are connected together at the centromere. The classic drawing of a chromosome shaped like the letter X actually depicts sister chromatids.