Elizabeth I dies and James VI of Scotland accedes to the English throne. Elizabeth I died childless so was succeeded by her cousin, James VI of Scotland, who henceforth assumed the title of James I of England as well.
T minus zero means out of time.. This comes from a countdown convention used in by both the American military and NASA. Generally, it is used when counting down to a major event that will happen at a specific time. Mathematically, T is time, minus whatever amount of time is left until the event happens.
1 tk, a gene that encodes thymidine kinase, an enzyme that phosphorylates the nucleoside analog ganciclovir. 2  DNA polymerase fails to discriminate against the resulting nucleotide and inserts this nonfunctional nucleotide into freshly-replicating DNA. 3  So ganciclovir kills cells that contain the tk gene.f the replacement gene (A* in the diagram) is nonfunctional (a null allele), mating of the heterozygous transgenic mice will produce a strain of  knockout mice  homozygous for the nonfunctional gene (both copies of the gene at that locus have been knocked out).