Whereas old dependency theory was Marxist in presentation, but nationalist in orientation, seeking to combine a theory of class struggle with a nationalist âthird worldistâ ideology, the new dependency theory is institutionalist in its analysis and political orientation.
Griffith concluded that the type II-R had been transformed into the lethal III-S strain by a transforming principle that was somehow part of the dead III-S strain bacteria. Today, we know that the transforming principle Griffith observed was the DNA of the III-s strain bacteria. While the bacteria had been killed, the DNA had survived the heating process and was taken up by the II-R strain bacteria. The III-S strain DNA contains the genes that form the protective polysaccharide capsule.
But these thermophile DNA polymerases work at 100C, a temperature at which DNA is denatured (in linear form). This thermophilic DNA polymerase is called Taq polymerase, named after T hermus aq uaticus, the bacteria it is derived from.Taq polymerase, however, has no proofreading ability. Other thermally stable polymerases, such as Vent and Pfu, have been discovered to both work for PCR and to proofread.his is the enzyme that is in charge of replicating DNA. This is the polymerase part of the name polymerase chain reaction. 4. Nucleotides. You'll need to add nucleotides (dNTPs) so the DNA polymerase has building blocks to work with.