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A Reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.It is mainly associated with retroviruses.elomerase is another reverse transcriptase found in many eukaryotes, including humans, which carries its own RNA template; this RNA is used as a template for DNA replication.
HIV-1 reverse transcriptase is one of the key players in the mechanism of infection by this retrovirus. The HIV-1 reverse transcriptase enzyme is responsible for copying a single-stranded viral RNA genome into double-stranded DNA (Sarafianos et al, 2001). The newly created DNA can then be incorporated into the host genome; the host is mainly the human in the case of HIV. The HIV-1 reverse transcriptase enzyme contains two main domains: a DNA polymerase domain and a ribonuclease H (RNase H) domain. The DNA polymerase is able to copy either an RNA or DNA template.