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A Reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.It is mainly associated with retroviruses.elomerase is another reverse transcriptase found in many eukaryotes, including humans, which carries its own RNA template; this RNA is used as a template for DNA replication.
reverse transcriptase (RT). [reversâ²tran-skripâ²tÄs]. an enzyme of RNA viruses that catalyzes the transcription of RNA to DNA, which is then incorporated into the genome of the host cell.This is the reverse of the usual mechanism for replication of genetic information; in the presence of this enzyme, it is the RNA that serves as the template for DNA copies.n enzyme of RNA viruses that catalyzes the transcription of RNA to DNA, which is then incorporated into the genome of the host cell.