Whereas old dependency theory was Marxist in presentation, but nationalist in orientation, seeking to combine a theory of class struggle with a nationalist âthird worldistâ ideology, the new dependency theory is institutionalist in its analysis and political orientation.
Griffith concluded that the type II-R had been transformed into the lethal III-S strain by a transforming principle that was somehow part of the dead III-S strain bacteria. Today, we know that the transforming principle Griffith observed was the DNA of the III-s strain bacteria. While the bacteria had been killed, the DNA had survived the heating process and was taken up by the II-R strain bacteria. The III-S strain DNA contains the genes that form the protective polysaccharide capsule.
T. aquaticus is a bacterium that lives in hot springs and hydrothermal vents, and Taq polymerase was identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. Therefore it replaced the DNA polymerase from E. coli originally used in PCR.e was familiar with the use of DNA oligonucleotides as probes for binding to target DNA strands, as well as their use as primers for DNA sequencing and cDNA synthesis. In 1983, he began using two primers, one to hybridize to each strand of a target DNA, and adding DNA polymerase to the reaction.