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A Reverse transcriptase (RT) is an enzyme used to generate complementary DNA (cDNA) from an RNA template, a process termed reverse transcription.It is mainly associated with retroviruses.elomerase is another reverse transcriptase found in many eukaryotes, including humans, which carries its own RNA template; this RNA is used as a template for DNA replication.
Reverse Transcriptases. Reverse transcriptase is a common name for an enzyme that functions as a RNA-dependent DNA polymerase. They are encoded by retroviruses, where they copy the viral RNA genome into DNA prior to its integration into host cells. DNA polymerase activity: In the retroviral life cycle, reverse transcriptase copies only RNA, but, as used in the laboratory, it will transcribe both single-stranded RNA and single-stranded DNA templates with essentially equivalent efficiency. 2  In both cases, an RNA or DNA primer is required to initiate synthesis.