In biology, the active site is the region of an enzyme where substrate molecules bind and undergo a chemical reaction. The active site consists of residues that form temporary bonds with the substrate (binding site) and residues that catalyse a reaction of that substrate (catalytic site).
Seattle is rolling out 11 rainbow crosswalks in Capitol Hill this week, adding a colorful bang to the annual Pride celebration. The crosswalks cost the city $66,000, said Seattle Department of Transportation spokesman Rick Sheridan, and are permanent.
Costs range from an average of approximately $750 for a striped crosswalk to nearly $2,600 for a high visibility crosswalk (Bushell, Poole, Zegeer, Rodriguez, 2013). Maintenance costs should also be considered based on the paint material used.