In biology, the active site is the region of an enzyme where substrate molecules bind and undergo a chemical reaction. The active site consists of residues that form temporary bonds with the substrate (binding site) and residues that catalyse a reaction of that substrate (catalytic site).
Seattle is rolling out 11 rainbow crosswalks in Capitol Hill this week, adding a colorful bang to the annual Pride celebration. The crosswalks cost the city $66,000, said Seattle Department of Transportation spokesman Rick Sheridan, and are permanent.
Colorbond sheds cost a little more than Zincalume, but come in more colours and you can combine colours for a more stylish shed. A shed with a Colorbond roof and walls might only cost $100 more than a Zincalume shed, so it's worth asking about your options. These sheds will come as is. If you want to add windows, insulation or interior cladding, you will have to do the work yourself or hire a builder to customise your shed. Other costs might include: 1  Concrete footings: from around $400 to $1900 depending on the size of the shed. 2  Concrete slab: from $70m2 to $100m2 depending on your location. 3  Construction: from around $60 per square metre. 4  Council fees may apply to larger or more expensive sheds.