In biology, the active site is the region of an enzyme where substrate molecules bind and undergo a chemical reaction. The active site consists of residues that form temporary bonds with the substrate (binding site) and residues that catalyse a reaction of that substrate (catalytic site).
Seattle is rolling out 11 rainbow crosswalks in Capitol Hill this week, adding a colorful bang to the annual Pride celebration. The crosswalks cost the city $66,000, said Seattle Department of Transportation spokesman Rick Sheridan, and are permanent.
Cantilever Sign: $15,000 to $20,000 each Sign Bridge: $30,000 to $60,000 each Foundations: Square Tube: $150 - $250 each Breakaway Post: $250 to $750 each Cantilever / Bridge: $6,000 - $7,000 each NOTE - if you're evaluating the replacement cost of an existing sign, you must take into account the extra labor and transportation costs involved.