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Quick Answer. Ethanol is used in DNA extraction to force the DNA to precipitate in a solution. In order to collect a DNA sample, cells are broken down through agitation, then mixed with water, salt and ethanol to create an aqueous solution.
Lysis of Cells. Lysis, or breaking open the cells, is the first step of DNA extraction. This is accomplished by a buffer containing tris and EDTA (ethylenediaminetetraacetic acid). EDTA binds divalent cations such as calcium and magnesium.ris as a Buffer. As pH can influence and be influenced by a number of cellular factors, maintaining a stable pH is essential to experimental science. Biological buffers, like tris, are important because they can maintain a stable pH despite influences that might otherwise shift the pH.