If you are well prepared for the laboratory, it is much less likely that an accident will occur. In the laboratory, you are responsible not only for your safety, but also for the safety of your classmates. If an accident happens because you are not prepared, it can also affect your friends. This is all the more reason for you to take the time and make the effort to prepare for the laboratory. Be sure to note the safety warnings listed in the Safety section of each experiment.
Fusion Splicing. Fusion splicing is the process of fusing or welding two fibers together usually by an electric arc. Fusion splicing is the most widely used method of splicing as it provides for the lowest loss and least reflectance, as well as providing the strongest and most reliable joint between two fibers. Virtually all singlemode splices are fusion.
Splicing of mRNA is performed by an RNA and protein complex known as the spliceosome, containing snRNPs designated U1, U2, U4, U5, and U6 (U3 is not involved in mRNA splicing). U1 binds to the 5' GU and U2, with the assistance of the U2AF protein factors, binds to the branchpoint A within the branch site.he Sxl protein is a splicing repressor that binds to an ISS in the RNA of the Tra transcript near the upstream acceptor site, preventing U2AF protein from binding to the polypyrimidine tract. This prevents the use of this junction, shifting the spliceosome binding to the downstream acceptor site.