An overloading of the gel with a soluble protein creates a semicircular band of this protein (e. g. in the marker lane of the image at 66 KDa), allowing other proteins with similar molecular weights to be covered.
Like arrowroot, tapioca also comes from the root of a plant, however, in this case it is the root of the Cassava plant. It is ground into powdered form and is often referred to as tapioca flour.
Flour is a powder made by grinding uncooked cereal grains or other seeds or roots (like cassava). It is the main ingredient of bread, which is a staple food for many cultures, making the availability of adequate supplies of flour a major economic and political issue at various times throughout history.