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Quick Answer. Ethanol is used in DNA extraction to force the DNA to precipitate in a solution. In order to collect a DNA sample, cells are broken down through agitation, then mixed with water, salt and ethanol to create an aqueous solution.
Tris, or tris(hydroxymethyl) aminomethane, is a common biological buffer, used throughout the DNA extraction process.During extraction from any number of sources, DNA is pH sensitive. During cell lysis, removal of unwanted cellular components and precipitation, tris is used to maintain a stable pH.ris as a Buffer. As pH can influence and be influenced by a number of cellular factors, maintaining a stable pH is essential to experimental science. Biological buffers, like tris, are important because they can maintain a stable pH despite influences that might otherwise shift the pH.