Answers. Best Answer: DNA extraction procedure: See number 8 re:chloroform 1.Treat 25 mg of tissue with Proteinase K by adding 20 ml of stock PK solution (20 mg/ml) and 180 ul water. Function: proteinase destroys protein. 2.Let sit at 55 degrees for at least 1 hour.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
Meyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.eyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.
Origin. The precise origin of the lemon tree (Citrus limon) is unknown, although some botanists believe it comes from Kashmir, north of India. Another possibility is that the lemon tree originated in southeast Asia, based on ancient documents that suggest a cultivation history of over 4,000 years.he Meyer Lemon Tree is named for Frank Meyer who introduced it to the United States from China in 1908. In the 1940s, the discovery that the Meyer Lemon Tree carried tristeza and tatter leaf viruses raised a red flag. In 1970, a new, virus free âImproved Meyer Lemonâ was introduced.