Answers. Best Answer: DNA extraction procedure: See number 8 re:chloroform 1.Treat 25 mg of tissue with Proteinase K by adding 20 ml of stock PK solution (20 mg/ml) and 180 ul water. Function: proteinase destroys protein. 2.Let sit at 55 degrees for at least 1 hour.unction: separates aqueous (top) and phenol (bottom) layers. 7.Remove upper aqueous layer (CONTAINS DNA) to a new tube. 8.Add 200 ul of chloroform to aqueous layer (IN HOOD). Function: removes excess phenol from DNA. 9.Cap tub; mix well by inverting. Function: sets an emulsion; biphasic solution.
Meyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.eyer lemons are popular backyard citrus trees. The improved Meyer lemon tree is a virus-free version of the Meyer lemon tree imported to the United States from China. The original Meyer tree was banned from commercial production decades ago.
Seasonal Care. Meyer lemon trees need less water in winter due to low light levels. The less light a tree receives, the longer it stays damp. This also holds true for potted Meyer lemon trees â if the potted tree is placed in an area of low light, it needs less water than a tree placed in full sunlight.Because Meyer lemon trees are designed to go dormant in the winter, you should also refrain from fertilizing them during the winter months.eyer lemon trees (Citrus x meyeri) are an excellent choice for hobby gardeners. These small trees (6 to 10 feet tall), which thrive in U.S. Department of Agriculture hardiness zones 9 to 11, pack a big punch and bear fruit at an earlier age than other comparable lemon trees.