Refer to Design Step 1 for introductory information about this design example. Additional information is presented about the design assumptions, methodology, and criteria for the entire bridge, including the steel girder. Figure 3-1 Span Configuration. Figure 3-2 Superstructure Cross Section.
HYPOFLUORESCENCE Hypofluorescence may be caused by 1) blocked fluorescence, which is synonymous with negative fluorescence or transmission decrease, or 2) a vascular filling defect. Blocked fluorescence corresponds to the size, shape, and location of the blocking material seen ophthalmoscopically.
Fluorophore detection in a given experiment can be obscured by high background fluorescence, which is most commonly caused by insufficient removal of nonbound fluorescent probe or sample autofluorescence. Thorough washing or a reduction in the concentration of fluorescent probe can reduce background fluorescence.