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As Aching pointed out the beep test or shuttle test uses an estimated VO2 max value. This relationship between shuttle level and VO2 max test has backed up by many studies over many years. It is a test where athletes who are used to change of speed and direction will perform best due to the specific demands of the test.
Brain natriuretic peptide (BNP) levels are simple and objective measures of cardiac function. These measurements can be used to diagnose heart failure, including diastolic dysfunction, and using them has been shown to save money in the emergency department setting. The high negative predictive value of BNP tests is particularly helpful for ruling out heart failure.