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As Aching pointed out the beep test or shuttle test uses an estimated VO2 max value. This relationship between shuttle level and VO2 max test has backed up by many studies over many years. It is a test where athletes who are used to change of speed and direction will perform best due to the specific demands of the test.
The test measures the level of a protein called alpha-fetoprotein (AFP) in your blood. It is a way to check for some types of birth defects. AFP is a protein and is normally produced by your baby in relatively high amounts. Some AFP passes from the baby into your bloodstream.