A Brief Explanation of an Over-the-Counter Market | Definition. A over-the-counter market (OTC) refers to a decentralized and informal way of exchanging stocks, derivatives, or other assets between two parties.
The leading strand is built by using the lagging strand of the old molecule as the template. Replication of the leading strand begins with the enzyme âprimaseâ. Primase adds a small complementary piece of RNA (~10 nucleotides), called an âRNA primerâ, near the end of the lagging strand. The RNA primer gives an enzyme called âDNA polymerase IIIâ something to attach a DNA nucleotide to. A jury-rigged design, RNA primers are necessary because DNA polymerase III can only attach nucleotides to the 3â end of a pre-existing nucleotide.
RNA polymerase holoenzyme contacts the promoter at roughly the centers of the two regions (-10 and -35) and the core enzyme tightly binds to the duplex DNA. Its action is that of melting the double-stranded DNA along a sequence of about 11 bps, from -9 to +2. The s factor splits off as transcription begins.