Heat shock transformation alters membrane fluidity creating pores: A sudden increase in temperature creates pores in the plasma membrane of the bacteria and allows for plasmid DNA to enter the bacterial cell.
Melanie Martinez is dating boyfriend Michael Keenan. Martinez and Keenanâs relationship had been suspected by a lot of their fans but, neither of them had confirmed it until 14th February 2016. She revealed about her relationship through her Twitter account where she designated Keenan as her boyfriend and fav producer.
Melanie Candy is a Minecraft player that used to be joined by Stampy and is also his ex-girlfriend. Stampy and Melanie used to go out but broke up late 2012. It even has a video where Stampy tells his fans that he and Melanie broke up. Stampy and Melanie used to make videos together like when they built a tardis in their own world and they also toured Bread Stick's world together. 2 years after, Stampy is now dating Sqaishey confirmed first by Sqaishey on a Q&A video, and then by Stampy on an episode of ear biscuits.