Heat shock transformation alters membrane fluidity creating pores: A sudden increase in temperature creates pores in the plasma membrane of the bacteria and allows for plasmid DNA to enter the bacterial cell.
Melanie Martinez is dating boyfriend Michael Keenan. Martinez and Keenanâs relationship had been suspected by a lot of their fans but, neither of them had confirmed it until 14th February 2016. She revealed about her relationship through her Twitter account where she designated Keenan as her boyfriend and fav producer.
In 2012, Melanie Adele Martinez became a contestant on season three of the American television program The Voice, where she auditioned singing Britney Spears 's  Toxic . Three of the four judges, Adam Levine, Cee Lo Green and Blake Shelton, hit the I Want You button for her. Martinez chose Levine to be her coach.