Heat shock transformation alters membrane fluidity creating pores: A sudden increase in temperature creates pores in the plasma membrane of the bacteria and allows for plasmid DNA to enter the bacterial cell.
Melanie Martinez is dating boyfriend Michael Keenan. Martinez and Keenanâs relationship had been suspected by a lot of their fans but, neither of them had confirmed it until 14th February 2016. She revealed about her relationship through her Twitter account where she designated Keenan as her boyfriend and fav producer.
Melanie Adele Martinez (born April 28, 1995 in Baldwin, New York) is an American singer, songwriter, and photographer of Puerto Rican and Dominican descent. She was a member of Team Adam in the third season of the American television vocal talent show The Voice. On April 22, 2014, she released the lead single Dollhouse, from her debut EP Dollhouse. On August 14, 2015, Martinez's debut album titled Cry Baby was released with lead single  Pity Party .