Heat shock transformation alters membrane fluidity creating pores: A sudden increase in temperature creates pores in the plasma membrane of the bacteria and allows for plasmid DNA to enter the bacterial cell.
Melanie Martinez is dating boyfriend Michael Keenan. Martinez and Keenanâs relationship had been suspected by a lot of their fans but, neither of them had confirmed it until 14th February 2016. She revealed about her relationship through her Twitter account where she designated Keenan as her boyfriend and fav producer.
Item removed from wishlist. This delightfully entertaining romantic comedy stars Reese Witherspoon as sophisticated Melanie Carmichael, a rising New York clothing designer who suddenly finds herself engaged to the city's most eligible bachelor. But this is no fairy tale romance for Melanie.