Heat shock transformation alters membrane fluidity creating pores: A sudden increase in temperature creates pores in the plasma membrane of the bacteria and allows for plasmid DNA to enter the bacterial cell.
Melanie Martinez is dating boyfriend Michael Keenan. Martinez and Keenanâs relationship had been suspected by a lot of their fans but, neither of them had confirmed it until 14th February 2016. She revealed about her relationship through her Twitter account where she designated Keenan as her boyfriend and fav producer.
Two and a Half Men star Melanie Lynskey -- who plays the neighbor Rose on the show -- has filed for divorce from her Always Sunny in Philadelphia star husband Jimmi Simpson, TMZ has learned. Melanie filed the docs on September 25th in L.A. County Superior Court, citing irreconcilable differences..