Heat shock transformation alters membrane fluidity creating pores: A sudden increase in temperature creates pores in the plasma membrane of the bacteria and allows for plasmid DNA to enter the bacterial cell.
Melanie Martinez is dating boyfriend Michael Keenan. Martinez and Keenanâs relationship had been suspected by a lot of their fans but, neither of them had confirmed it until 14th February 2016. She revealed about her relationship through her Twitter account where she designated Keenan as her boyfriend and fav producer.
February 10, 2014. She didn't make it to the finals on The Voice's third season, but New York native Melanie Martinez might be the NBC show's first real breakout star. The 18-year-old former Team Adam Levine contestant has released her debut single Dollhouse on iTunes, and posted an accompanying music video.