Heat shock transformation alters membrane fluidity creating pores: A sudden increase in temperature creates pores in the plasma membrane of the bacteria and allows for plasmid DNA to enter the bacterial cell.
Melanie Martinez is dating boyfriend Michael Keenan. Martinez and Keenanâs relationship had been suspected by a lot of their fans but, neither of them had confirmed it until 14th February 2016. She revealed about her relationship through her Twitter account where she designated Keenan as her boyfriend and fav producer.
During a recent interview with Angie Martinez, Jamie recalled a hilarious situation where his daughter met one of his girlfriends who she felt was way too young for him. âIt was this one time where I was dating this girl who was too young a while back. She was legal, but in her 20s. Iâm 40, sheâs in her 20s.