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These fibers receive messages from the brain, causing the fibers to contract. The main muscles at work in cycling are the quadriceps and hamstrings in the upper leg, and the gastrocnemius and soleus in the calf. These muscles contract in a sequence that creates the pedaling action.The quadriceps and hamstrings do most of the work when you ride a bicycle.hese fibers receive messages from the brain, causing the fibers to contract. The main muscles at work in cycling are the quadriceps and hamstrings in the upper leg, and the gastrocnemius and soleus in the calf. These muscles contract in a sequence that creates the pedaling action.
In the study discussion notes, the researchers remarked that the hamstrings and quadriceps are used more on a recumbent bike than an upright bike. On a recumbent bike, your legs are primarily responsible for powering the machine, but on an upright bike, your core and upper body share the work load with your legs.