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How to read the Histogram. A histogram is a graphical representation of the pixels exposed in your image. The left side of the graph represents the blacks or shadows, the right side represents the highlights or bright areas and the middle section is mid-tones (middle or 18% grey).
The X-axis is the amount of red fluorescence. The more red fluorescence a cell emits, the farther to the right the cell data will appear on the histogram. The Y-axis is the amount of blue fluorescence.