Heat shock transformation alters membrane fluidity creating pores: A sudden increase in temperature creates pores in the plasma membrane of the bacteria and allows for plasmid DNA to enter the bacterial cell.
Melanie Martinez is dating boyfriend Michael Keenan. Martinez and Keenanâs relationship had been suspected by a lot of their fans but, neither of them had confirmed it until 14th February 2016. She revealed about her relationship through her Twitter account where she designated Keenan as her boyfriend and fav producer.
Melanie Martinez is a singer with an amazing and unique quality of voice, best described as ethereal, breathy, even magical, in her own brand of indie sound. She is recognized by her signature two tone bangs and a gap in her teeth. She auditioned on the Voice with an alternative version of Toxic, during which she played the tambourine with her feet, getting her a spot on Team Adam.