Heat shock transformation alters membrane fluidity creating pores: A sudden increase in temperature creates pores in the plasma membrane of the bacteria and allows for plasmid DNA to enter the bacterial cell.
Melanie Martinez is dating boyfriend Michael Keenan. Martinez and Keenanâs relationship had been suspected by a lot of their fans but, neither of them had confirmed it until 14th February 2016. She revealed about her relationship through her Twitter account where she designated Keenan as her boyfriend and fav producer.
Antonio Banderas (left) and Melanie Griffith are ending their relationship of 18 years, the two said in a statement on Friday. Antonio Banderas and Melanie Griffith were no longer in love with each other after 18 years of marriage, reports Us Weekly. Their split, which the couple announced on Friday, was a long time coming, a source told the mag. There was no cheating on either side, the insider said.