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WHAT IS UV-C. Ultraviolet light in the C spectrum (UV-C) is energy-rich light with a wavelength of 200 â 400 nanometres (nm). UV-C light is very versatile and can be used for disinfecting water, destroying harmful micro-organisms in other liquids, on surfaces, on food products and in 'air'.
UV melting. Heating double stranded nucleic acids causes the strands to unwind (denature) by disrupting the ordered stacking of the bases and breaking hydrogen bonds. The process can be conveniently monitored by an increase in UV absorbance as the double strands unwind to single strands (owing to hypochromicity).