Typical costs: 1  Costs for a groom's cake are similar to those for wedding cakes, ranging from $1-$2 or less per slice for a relatively straightforward single-tier or sheet cake, to $8-$10 or more a slice for elaborate cakes with fondant, gum paste and sugar decorations or fruit.
Immunofluorescence microscopy is used to localize specific constituents in tissue sections or immobilized cells using fluorescent tags as labels.
1 Primary, or direct, immunofluorescence uses a single antibody that is conjugated directly to a fluorescent dye. 2  The antibody recognizes the target molecule, binds to it and the conjugated fluorescent dye can be detected by the microscope.here are two classes of immunofluorescence techniques, primary (or direct) and secondary (or indirect). 1  Primary (direct). 2  Primary, or direct, immunofluorescence uses a single antibody that is conjugated directly to a fluorescent dye.