Replaced blower fan resistor today without removing dash. Dealer wanted 10 hours labor to replace resistor. Took me two hours, next one could do in hour. 4 of 5 fan screws you can get to without problems. For 5th screw which requires removal of dash, I just pulled fan down and mount broke right off.
1 Answer 1. If I understand the nomenclature correctly, an R plasmid is just any plasmid containing an antibiotic (R)esistance gene (eg. Amp, Kan, Cm, etc.). It's a bit of an outdated name from when people didn't know how exactly the plasmids conferred such resistance.
The pGLO plasmid contains an origin or replication, a selectable marker, and the gene for Green Fluorescent Protein (GFP). The plasmid also contains a gene for the arabinose C protein, which is a protein that regulates expression from the arabinose BAD promoter (PBAD).