Typical costs: 1  Costs for a groom's cake are similar to those for wedding cakes, ranging from $1-$2 or less per slice for a relatively straightforward single-tier or sheet cake, to $8-$10 or more a slice for elaborate cakes with fondant, gum paste and sugar decorations or fruit.
Immunofluorescence microscopy is used to localize specific constituents in tissue sections or immobilized cells using fluorescent tags as labels.
This works because the emitted light is of lower energy and has a longer wavelength than the light that is used for illumination. Most of the fluorescence microscopes used in biology today are epi-fluorescence microscopes, meaning that both the excitation and the observation of the fluorescence occur above the sample.