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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
Before DNA Replication (S Phase) - A chromosome. After DNA Replication - a duplicated chromosome, made up of 2 sister chromatids. The sister chromatids are pulled apart during mitosis (division of the nucleus), and partitioned into the 2 new daughter cells by cytokinesis (division of the cell). Result of mitosis: two daughter cells genetically identical to the parent cell.