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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
Nuclear and Cellular Division. During nuclear division, the chromosomes line up at the cell's equator and then the sister chromatids separate and move to opposite ends of the cell (poles). Once separated these former sister chromatids are now each considered a chromosome.Once the chromosomes move to opposite poles, the dividing cell reforms a nuclear membrane around each of the two areas of separated genetic material.ach of the two copies of a chromosome are designated as a sister chromatid and they are physically linked together at a point on the chromosome called the centromere. Students often become confused when it comes to the genetics terminology associated with chromosomes and chromosome number.