Replaced blower fan resistor today without removing dash. Dealer wanted 10 hours labor to replace resistor. Took me two hours, next one could do in hour. 4 of 5 fan screws you can get to without problems. For 5th screw which requires removal of dash, I just pulled fan down and mount broke right off.
1 Answer 1. If I understand the nomenclature correctly, an R plasmid is just any plasmid containing an antibiotic (R)esistance gene (eg. Amp, Kan, Cm, etc.). It's a bit of an outdated name from when people didn't know how exactly the plasmids conferred such resistance.
pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors.Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after the postdoctoral researchers who constructed it.The p stands for plasmid, and BR for Bolivar and Rodriguez..he plasmid has unique restriction sites for more than forty restriction enzymes. 11 of these 40 sites lie within the tetR gene. There are 2 sites for restriction enzymes HindIII and ClaI within the promoter of the tetR gene. There are 6 key restriction sites inside the ampR gene.