mycobacteria and fungi: thick cell walls, difficult to lyse than bacteria and parasite. GP bacteria have thick cell walls than GN bacteria and may require more rigorous cell lysis condition. mycoplasma lacks a cell wall, care must betaken to avoid spontaneous lysis of cells and loss of NA.
The Average Cost of a Bachelor's Degree. The average annual tuition and fees for a four-year bachelor's degree in the United States is $8,893 for in-state attendees of public colleges, $22,203 for out-of-state attendees of public colleges, and $30,094 for private nonprofit colleges.
Because of this, the average cost of getting a college degree is highly dependent on the individual college, choice of major, and whether tuition is in-state or out-of-state; this difference in expenses can be quite large, ranging from $0 to upwards of $65,000 per year.