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A full set of sister chromatids is created during the S subphase of interphase, when all the DNA in a cell is replicated. Identical chromosome pairs are separated into two different cells during mitosis, or cellular division.
What happens during the first stage of the cell cycle in a eukaryotic cell? In the first stage, called interphase, the cell grows and copies its organelles and chromosomes. After each chromosomes is duplicated, the two copies are called chromatids. 42 people found this useful.