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Best Answer: Melting point of DNA is related to the GC content of it. Since GC forms three hydrogen bonds, while AT forms two hydrogen bonds, the more GC you have in your DNA the higher the melting point will be.If the GC content is about 50%, the melting point is around 50C.The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.f the GC content is about 50%, the melting point is around 50C. The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.
Best Answer: Melting temp is when DNA strands separate, annealing temp is the temp where complimentary strands recombine (or anneal).The importance is this: In PCR, the temperature is cylced high to low, and a DNA polymerase that is heat-resistant is used so that it is not denatured at higher temps.f you don't reduce it to that lower temperature, the primers can never hydrogen bond with the DNA and cause extension. and this annealing temperature is CRUCIAL for the PCR to work.