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Best Answer: Melting point of DNA is related to the GC content of it. Since GC forms three hydrogen bonds, while AT forms two hydrogen bonds, the more GC you have in your DNA the higher the melting point will be.If the GC content is about 50%, the melting point is around 50C.The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.f the GC content is about 50%, the melting point is around 50C. The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.
THEME Deoxyribonucleic Acid (DNA)DNA is normally double-stranded and the two strands are held together by weak interactions, chiefly stacking interactions and hydrogen bonds [DNA Denaturation and Renaturation and the Role of Hydrogen Bonds and Stacking Interactions].he figure shows that the DNA molecules melt, or denature, as the temperature is raised. When the temperature was lowered to 67Â°C they could follow the formation of double-standed DNA over time. For virus and bacteriophage DNA (solid triangles and circles) most of the DNA seemed to renature in about one hour.