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Best Answer: Melting point of DNA is related to the GC content of it. Since GC forms three hydrogen bonds, while AT forms two hydrogen bonds, the more GC you have in your DNA the higher the melting point will be.If the GC content is about 50%, the melting point is around 50C.The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.f the GC content is about 50%, the melting point is around 50C. The exact number is hard to calculate and there really isn't a universally accepted way of calculating melting temp base on the sequence. You can monitor DNA melting through UV light.
Both DNA melting (LCGreen Plus, excitation at 450 nm, emission at 500 nm) and temperature (sulforhodamine B, excitation at 558 nm, emission at 610 nm) were monitored simultaneously by fluorescence during heating of the sample from 60 to 95 Â°C.