Largetooth sawfish
Adults are primarily found in estuaries and marine waters to a depth of , but mostly less than . Nevertheless, the species does appear to have a greater affinity for freshwater habitats than the smalltooth sawfish ("P. pectinata"), green sawfish ("P. zijsron"), and dwarf sawfish ("P. clavata"). Largetooth sawfish from the population in Lake Nicaragua appear to spend most, if not all, of their life in freshwater, but tagging surveys indicate that at least some do move between this lake and the sea. Captive studies show that this euryhaline species can thrive long-term in both salt and freshwater, regardless of its age, and that an acclimation from salt to freshwater is faster than the opposite. In captivity they are known to be agile (even swimming backwards), have an unusual ability to "climb" with the use of the pectoral fins and they can jump far out of the water; a individual jumped to a height of . It has been suggested that this may be adaptions for traversing medium-sized waterfalls and rapids when moving upriver. They are generally found in areas with a bottom consisting of sand, mud or silt. The preferred water temperature is between , and or colder is lethal.
Satinfin shiner
It is a freshwater fish that inhabits rivers and creeks, sometimes entering pools and riffles, and found from headwaters to areas near tidal influence. It eats insects, especially flies and mayflies. Spawning occurs in spring and summer, with individuals spawning several times over a season. The eggs are laid in cracks and crevices, under rocks, and on tree roots and wood debris. The male guards them until the young emerge in about one to two weeks, depending on temperature. The lifespan of the species is 3 to 4 years.