Ay
Egyptologist Bob Brier suggested that Ay murdered Tutankhamun in order to usurp the throne, a claim which was based on X-ray examinations of the body done in 1968. He also alleged that Ankhesenamun and the Hittite prince she was about to marry were also murdered at his orders. This murder theory was not accepted by all scholars, and more detailed CT scans of the mummy undertaken by National Geographic (published in late 2005) suggested that Tutankhamun did not die from a blow to his head as Brier had theorized. The "National Geographic" forensic researchers instead presented a new theory that Tutankhamun died from an infection caused by a badly broken leg since he is often portrayed as walking with a cane due to spina bifida, a hereditary trait in his family on his father's side. The bone fragments found in Tutankhamun's skull were most likely the result of post-mortem damage caused by Howard Carter's initial examination of the boy king "because they show no evidence of being inundated with the embalming fluid used to preserve the pharaoh for the afterlife". However, Brier has stated that the bone fragment in the skull is not relevant to the issue of whether Tutankhamun was murdered, acknowledging that it was likely caused by the embalmers. The evidence Brier presents for the murder is a dark spot on the base of the skull, indicating a blow to the head. Dr. Gerald Irwin agrees with Brier on this point.
Tutankhamun
In 2008, a team began DNA research on Tutankhamun and the mummified remains of other members of his family. The results indicated that his father was Akhenaten, and that his mother was not one of Akhenaten's known wives but one of his father's five sisters. The techniques used in the study, however, have been questioned. The team reported it was over 99.99 percent certain that Amenhotep III was the father of the individual in KV55, who was in turn the father of Tutankhamun. The young king's mother was found through the DNA testing of a mummy designated as 'The Younger Lady' (KV35YL), which was found lying beside Queen Tiye in the alcove of KV35. Her DNA proved that, like his father, she was a child of Amenhotep III and Tiye; thus, Tutankhamun's parents were brother and sister. Queen Tiye held much political influence at court and acted as an adviser to her son after the death of her husband. Some geneticists dispute these findings, however, and "complain that the team used inappropriate analysis techniques."
While the data are still incomplete, the study suggests that one of the mummified fetuses found in Tutankhamun's tomb is the daughter of Tutankhamun himself, and the other fetus is probably his child as well. So far, only partial data for the two female mummies from KV21 has been obtained. One of them, KV21A, may be the infants' mother, and, thus, Tutankhamun's wife, Ankhesenamun. It is known from history that she was the daughter of Akhenaten and Nefertiti, and thus likely to be her husband's half-sister. One consequence of inbreeding can be children whose genetic defects do not allow them to be brought to term.