Ridley (Metroid)
Often considered one of the most memorable Nintendo villains, Ridley has been a mainstay of the series, having appeared (through various incarnations) in almost every "Metroid" game to date (all but "", "Metroid Prime Hunters" and ""). He has also appeared in the "Super Smash Bros." series, however, he had not been made a playable character until "Super Smash Bros. Ultimate" in 2018, making it also the first game overall to have Ridley as a playable character.
Trigger Twins
As with many other villains, the twins join up with the Secret Society of Super Villains. As part of an army, they are sent to destroy the city of Metropolis. Another army of superheroes face them. During what is called "Battle of Metropolis", the twins are shot down in the street by a group of vigilantes that include the current Vigilante and Wild Dog who were perched on a nearby rooftop. This battle takes place in "Infinite Crisis" #7.