Ay
Egyptologist Bob Brier suggested that Ay murdered Tutankhamun in order to usurp the throne, a claim which was based on X-ray examinations of the body done in 1968. He also alleged that Ankhesenamun and the Hittite prince she was about to marry were also murdered at his orders. This murder theory was not accepted by all scholars, and more detailed CT scans of the mummy undertaken by National Geographic (published in late 2005) suggested that Tutankhamun did not die from a blow to his head as Brier had theorized. The "National Geographic" forensic researchers instead presented a new theory that Tutankhamun died from an infection caused by a badly broken leg since he is often portrayed as walking with a cane due to spina bifida, a hereditary trait in his family on his father's side. The bone fragments found in Tutankhamun's skull were most likely the result of post-mortem damage caused by Howard Carter's initial examination of the boy king "because they show no evidence of being inundated with the embalming fluid used to preserve the pharaoh for the afterlife". However, Brier has stated that the bone fragment in the skull is not relevant to the issue of whether Tutankhamun was murdered, acknowledging that it was likely caused by the embalmers. The evidence Brier presents for the murder is a dark spot on the base of the skull, indicating a blow to the head. Dr. Gerald Irwin agrees with Brier on this point.
Akhenaten
Similarly, although it is accepted that Akhenaten himself died in Year 17 of his reign, the question of whether Smenkhkare became co-regent perhaps two or three years earlier or enjoyed a brief independent reign is unclear. If Smenkhkare outlived Akhenaten, and became sole pharaoh, he likely ruled Egypt for less than a year. The next successor was Neferneferuaten, a female pharaoh who reigned in Egypt for two years and one month. She was, in turn, probably succeeded by Tutankhaten (later, Tutankhamun), with the country being administered by the chief vizier, and future pharaoh, Ay. Tutankhamun was believed to be a younger brother of Smenkhkare and a son of Akhenaten, and possibly Kiya although one scholar has suggested that Tutankhamun may have been a son of Smenkhkare instead. DNA tests in 2010 indicated Tutankhamun was indeed the son of Akhenaten. It has been suggested that after the death of Akhenaten, Nefertiti reigned with the name of Neferneferuaten but other scholars believe this female ruler was rather Meritaten. The so-called Coregency Stela, found in a tomb in Amarna possibly shows his queen Nefertiti as his coregent, ruling alongside him, but this is not certain as the names have been removed and recarved to show Ankhesenpaaten and Neferneferuaten.