Ridley (Metroid)
Often considered one of the most memorable Nintendo villains, Ridley has been a mainstay of the series, having appeared (through various incarnations) in almost every "Metroid" game to date (all but "", "Metroid Prime Hunters" and ""). He has also appeared in the "Super Smash Bros." series, however, he had not been made a playable character until "Super Smash Bros. Ultimate" in 2018, making it also the first game overall to have Ridley as a playable character.
Brotherhood of Evil
With Madame Rouge's daughter Gemini now a member, the group reunited with Phobia, Mallah, and Brain and joined the Secret Society of Super Villains, under the command of inner circle member Deathstroke. Deathstroke had the group acquire the mass murdering chemical monster Chemo for the Society and in "Infinite Crisis" #4, dropped the monster onto the city of Blüdhaven. Murdering several million people, the Brotherhood became the most wanted villains in the DC Universe for their act of mass murder. Furthermore, Warp was recruited by the villainous Doctor Psycho to free the murderous Doomsday for the Society as part of the group's final endgame against Earth's heroes.