Astronaut
The first civilian in space was Valentina Tereshkova aboard Vostok 6 (she also became the first woman in space on that mission).
Tereshkova was only honorarily inducted into the USSR's Air Force, which did not accept female pilots at that time. A month later, Joseph Albert Walker became the first American civilian in space when his X-15 Flight 90 crossed the line, qualifying him by the international definition of spaceflight. Walker had joined the US Army Air Force but was not a member during his flight. 
The first people in space who had never been a member of any country's armed forces were both Konstantin Feoktistov and Boris Yegorov aboard Voskhod 1.
JoAnn H. Morgan
JoAnn Hardin Morgan (December 4, 1940) is an American aerospace engineer who was a trailblazer in the United States space flight program as the first female engineer at the National Aeronautics and Space Administration (NASA) John F. Kennedy Space Center and the first woman to serve as a senior executive at Kennedy Space Center. For her work at NASA, Morgan was honored by U.S. President Bill Clinton as a Meritorious Executive in 1995 and 1998. Prior to her retirement in 2003, she held various leadership positions over 40 years in the manned space flight programs at NASA. Morgan served as the director of the External Relations and Business Development during her final years at the space center.