Largetooth sawfish
Adults are primarily found in estuaries and marine waters to a depth of , but mostly less than . Nevertheless, the species does appear to have a greater affinity for freshwater habitats than the smalltooth sawfish ("P. pectinata"), green sawfish ("P. zijsron"), and dwarf sawfish ("P. clavata"). Largetooth sawfish from the population in Lake Nicaragua appear to spend most, if not all, of their life in freshwater, but tagging surveys indicate that at least some do move between this lake and the sea. Captive studies show that this euryhaline species can thrive long-term in both salt and freshwater, regardless of its age, and that an acclimation from salt to freshwater is faster than the opposite. In captivity they are known to be agile (even swimming backwards), have an unusual ability to "climb" with the use of the pectoral fins and they can jump far out of the water; a individual jumped to a height of . It has been suggested that this may be adaptions for traversing medium-sized waterfalls and rapids when moving upriver. They are generally found in areas with a bottom consisting of sand, mud or silt. The preferred water temperature is between , and or colder is lethal.
Squalomorphii
The order Pristiophoriformes is a small order of unique sharks called sawsharks. Sawsharks have a long snout covered in teeth, between 5-6 gill slits, and no anal fin. They also have a pair of barbels usually located halfway down the snout. Sawsharks are frequently confused with sawfish, which are members of the ray order Pristiformes. Sawsharks can be discerned from sawfish thanks to their lateral gill slits, which differ from the sawfish's ventral gill slits. These sharks can mostly be found in deep benthic environments. Fossils of members of this order have been found since the late Jurassic period.