Ay
Egyptologist Bob Brier suggested that Ay murdered Tutankhamun in order to usurp the throne, a claim which was based on X-ray examinations of the body done in 1968. He also alleged that Ankhesenamun and the Hittite prince she was about to marry were also murdered at his orders. This murder theory was not accepted by all scholars, and more detailed CT scans of the mummy undertaken by National Geographic (published in late 2005) suggested that Tutankhamun did not die from a blow to his head as Brier had theorized. The "National Geographic" forensic researchers instead presented a new theory that Tutankhamun died from an infection caused by a badly broken leg since he is often portrayed as walking with a cane due to spina bifida, a hereditary trait in his family on his father's side. The bone fragments found in Tutankhamun's skull were most likely the result of post-mortem damage caused by Howard Carter's initial examination of the boy king "because they show no evidence of being inundated with the embalming fluid used to preserve the pharaoh for the afterlife". However, Brier has stated that the bone fragment in the skull is not relevant to the issue of whether Tutankhamun was murdered, acknowledging that it was likely caused by the embalmers. The evidence Brier presents for the murder is a dark spot on the base of the skull, indicating a blow to the head. Dr. Gerald Irwin agrees with Brier on this point.
Tutankhamun's mummy
In 1968, R. G. Harrison, a professor of anatomy, used a portable x-ray machine to get a better look at the internal structures of the mummy to better determine age and cause of death of Tutankhamun. One of the most abnormal findings was the sternum (breastbone) and most parts of the frontal ribs were missing. Removing these bones was not part of the normal mummification process, which led Harrison to believe they might have been removed because they were badly damaged before his death. Harrison quickly discovered that Carter was not as careful as many of his personal notes had claimed. The mummy was not re-wrapped after 1926, which led to more deterioration due to the extremely hot external elements over the forty-two years. Also many of the limbs had been amputated in the body in order to remove some of the jewellery. Both hands were cut off, both legs were removed from the pelvis, and the head was severed from the body in order to get the mask off. Even more remarkable is the king's right ear and penis were missing, but photographs from Carter show they were both present during his examination. Harrison believed the slight curve in the spine and small bone fragments might have been the result of the embalming process. The lesion on the left jaw showed signs of healing occurred before his death and one of his legs had been broken, but it could not be determined if it happened naturally or as a result of the embalming or Carter's examination. The fact that skull fragments were discovered led many to assume the king was murdered by a blow to the head, but the x-ray could not support or discredit this theory.