Manuel I of Portugal
Manuel's mother was the granddaughter of King John I of Portugal, whereas his father was the second surviving son of King Edward of Portugal and the younger brother of King Afonso V of Portugal. In 1495, Manuel succeeded his first cousin, King John II of Portugal, who was also his brother-in-law, as husband to Manuel's sister, Eleanor of Viseu.
Maria Manuela, Princess of Portugal
Dona Maria Manuela (15 October 1527 – 12 July 1545) was the eldest daughter and second child of King John III of Portugal and his wife Catherine of Austria. She was Princess of Asturias and Duchess of Milan as the first wife of the future Philip II of Spain, and Princess of Portugal as heir presumptive to the Portuguese throne between 1527 and 1535.