Wolves as pets and working animals
Some wildlife centers housing captive wolves prohibit handlers from entering wolf enclosures if they happen to have a cold or other vulnerability which the wolves can detect. Captive wolves are generally shy and avoid eye contact with humans other than their owner, as well as not listening to any commands made by any other humans. They usually vacate rooms or hide when a new person enters the establishment. Even seemingly friendly wolves need to be treated with caution, as captive wolves tend to view and treat people as other wolves, and will thus bite or dominate people in the same situation in which they would other wolves. Ordinary pet food is inadequate, as an adult wolf needs 1–2.5 kg (2–5 lbs) of meat daily along with bones, skin and fur to meet its nutritional requirements. Wolves may defend their food against people, and react violently to people trying to remove it. The exercise needs of a wolf exceed the average dog's demand. Because of this, captive wolves typically do not cope well in urban areas. Due to their talent at observational learning, adult captive wolves can quickly work out how to escape confinement, and require constant reinforcement by caretakers or owners, which makes raising wolves difficult for people who raise their pets in an even, rather than subordinate, environment.
Red wolf
Genetic studies relating to wolves or dogs have inferred phylogenetic relationships based on the only reference genome available, that of the Boxer dog. In 2017, the first reference genome of the wolf "Canis lupus lupus" was mapped to aid future research. In 2018, a study looked at the genomic structure and admixture of North American wolves, wolf-like canids, and coyotes using specimens from across their entire range that mapped the largest dataset of nuclear genome sequences against the wolf reference genome. The study supports the findings of previous studies that North American gray wolves and wolf-like canids were the result of complex gray wolf and coyote mixing. A polar wolf from Greenland and a coyote from Mexico represented the purest specimens. The coyotes from
Alaska, California, Alabama, and Quebec show almost no wolf ancestry. Coyotes from Missouri, Illinois, and Florida exhibit 5–10% wolf ancestry. There was 40%:60% wolf to coyote ancestry in red wolves, 60%:40% in Eastern timber wolves, and 75%:25% in the Great Lakes wolves. There was 10% coyote ancestry in Mexican wolves and the Atlantic Coast wolves, 5% in Pacific Coast and Yellowstone wolves, and less than 3% in Canadian archipelago wolves.