Atomic mass
One mole of a substance always contains almost exactly the "standard atomic weight" or "molar mass" of that substance; however, this may or may not be true for the "atomic mass," depending on whether or not the element exists naturally in more than one isotope. For example, the standard atomic weight of iron is 55.847 g/mol, and therefore one mole of iron as commonly found on earth has a mass of 55.847 grams. The "atomic mass" of the Fe isotope is 55.935 u and one mole of Fe atoms would then in theory have a mass of 55.935 g, but such amounts of pure Fe have never been found (or separated out) on Earth. However, there are 22 mononuclidic elements of which essentially only a single isotope is found in nature (common examples are fluorine, sodium, aluminum and phosphorus) and for these elements the standard atomic weight and atomic mass are the same. Samples of these elements therefore may serve as reference standards for certain atomic mass values.
Mass–energy equivalence
The difference between the rest mass of a bound system and of the unbound parts is the binding energy of the system, if this energy has been removed after binding. For example, a water molecule weighs a little less than two free hydrogen atoms and an oxygen atom. The minuscule mass difference is the energy needed to split the molecule into three individual atoms (divided by ), which was given off as heat when the molecule formed (this heat had mass). Likewise, a stick of dynamite in theory weighs a little bit more than the fragments after the explosion, but this is true only so long as the fragments are cooled and the heat removed. In this case the mass difference is the energy/heat that is released when the dynamite explodes, and when this heat escapes, the mass associated with it escapes, only to be deposited in the surroundings, which absorb the heat (so that total mass is conserved).