Atomic mass
Any mass defect due to nuclear binding energy is experimentally a small fraction (less than 1%) of the mass of equal number of free nucleons. When compared to the average mass per nucleon in carbon-12, which is moderately strongly-bound compared with other atoms, the mass defect of binding for most atoms is an even smaller fraction of a dalton (unified atomic mass unit, based on carbon-12). Since free protons and neutrons differ from each other in mass by a small fraction of a dalton (about 0.0014 u), rounding the relative isotopic mass, or the atomic mass of any given nuclide given in daltons to the nearest whole number always gives the nucleon count, or mass number. Additionally, the neutron count (neutron number) may then be derived by subtracting the number of protons (atomic number) from the mass number (nucleon count).
Mass
In bound systems, the binding energy must often be subtracted from the mass of the unbound system, because binding energy commonly leaves the system at the time it is bound. The mass of the system changes in this process merely because the system was not closed during the binding process, so the energy escaped. For example, the binding energy of atomic nuclei is often lost in the form of gamma rays when the nuclei are formed, leaving nuclides which have less mass than the free particles (nucleons) of which they are composed.