Mitochondrial DNA
Mitochondrial DNA was discovered in the 1960s by Margit M. K. Nass and Sylvan Nass by electron microscopy as DNase-sensitive threads inside mitochondria, and by Ellen Haslbrunner, Hans Tuppy and Gottfried Schatz by biochemical assays on highly purified mitochondrial fractions.
Multiregional origin of modern humans
A 1987 analysis of mitochondrial DNA from 147 people by Cann "et al". from around the world indicated that their mitochondrial lineages all coalesced in a common ancestor from Africa between 140,000 and 290,000 years ago. The analysis suggested that this reflected the worldwide expansion of modern humans as a new species, replacing, rather than mixing with, local archaic humans outside of Africa. Such a recent replacement scenario is not compatible with the Multiregional hypothesis and the mtDNA results led to increased popularity for the alternative single replacement theory. According to Wolpoff and colleagues: