Lawrence, Kansas
Prior to Kansas Territory being established in May 1854, most of Douglas County was part of the Shawnee Indian Reservation (created in 1830). During this period, the Oregon Trail ran parallel to the Kansas River, roughly through the area where Lawrence would eventually be situated and a hill known then as "Hogback Ridge" (i.e., Mount Oread, which sits on the water divide separating the Kansas and Wakarusa River). This area was used as a landmark and an outlook by those on the trail. While this territory was technically unopened to settlement prior to 1854, there did exist a few "squatter settlements" in the area, especially just north of the Kansas River.
25th meridian west from Washington
On January 1, 1861, the Act Admitting the State of Kansas to the Union defined the western boundary of the new state as the 25th meridian of longitude west from Washington. This rendered the western portion of the Territory of Kansas unorganized. Thirty days later on February 28, 1861, the Act Organizing the Territory of Colorado defined the eastern boundary of the new territory as the 25th meridian of longitude west from Washington. The creation of the Colorado Territory moved the western boundary of the Territory of Nebraska south of the 41st parallel north east to this meridian. These boundaries on the 25th meridian of longitude west from Washington remained when Nebraska became a state on March 1, 1867, and Colorado became a state on August 1, 1876.