Chameleon (comics)
Created by writer Stan Lee and artist Steve Ditko, the character first appeared in "The Amazing Spider-Man" #1 (March 1963), making him the first member of Spider-Man's rogues' gallery, based on issue publication date, excluding the burglar who murdered Ben Parker (Supercharger was the first Spider-Man supervillain chronologically). He is also well known to be related to Kraven the Hunter. That revealed relationship helped evolve him as a major villain compared to his original depiction of being just a solo villain in the original issue of "The Amazing Spider-Man".
Kraven the Hunter (Ana Kravinoff)
Ana Kravinoff, also known as the third Kraven the Hunter, is a fictional supervillainess appearing in American comic books published by Marvel Comics. She is the daughter of Kraven the Hunter, the niece of Chameleon, the sister of Grim Hunter, and the half-sister of Alyosha Kravinoff.