Van de Graaff generator
Other electrostatic machines like the Wimshurst machine or Bonetti machine work similarly to the Van De Graaff; charge is transported by moving plates, disks, or cylinders to a high voltage electrode. For these generators, however, corona discharge from exposed metal parts at high potentials and poorer insulation result in smaller voltages. In an electrostatic generator, the rate of charge transported (current) to the high-voltage electrode is very small. After the machine is started, the voltage on the terminal electrode increases until the leakage current from the electrode equals the rate of charge transport. Therefore, leakage from the terminal determines the maximum voltage attainable. In the Van de Graaff generator, the belt allows the transport of charge into the interior of a large hollow spherical electrode. This is the ideal shape to minimize leakage and corona discharge, so the Van de Graaff generator can produce the greatest voltage. This is why the Van de Graaff design has been used for all electrostatic particle accelerators. In general, the larger the diameter and the smoother the sphere is, the higher the voltage that can be achieved.
Electroporation
Electroporation is performed with electroporators, purpose-built appliances which create an electrostatic field in a cell solution. The cell suspension is pipetted into a glass or plastic cuvette which has two aluminium electrodes on its sides. For bacterial electroporation, typically a suspension of around 50 microliters is used. Prior to electroporation, this suspension of bacteria is mixed with the plasmid to be transformed. The mixture is pipetted into the cuvette, the voltage and capacitance are set, and the cuvette is inserted into the electroporator. The process requires direct contact between the electrodes and the suspension. Immediately after electroporation, one milliliter of liquid medium is added to the bacteria (in the cuvette or in an Eppendorf tube), and the tube is incubated at the bacteria's optimal temperature for an hour or more to allow recovery of the cells and expression of the plasmid, followed by bacterial culture on agar plates.