Chameleon (comics)
Created by writer Stan Lee and artist Steve Ditko, the character first appeared in "The Amazing Spider-Man" #1 (March 1963), making him the first member of Spider-Man's rogues' gallery, based on issue publication date, excluding the burglar who murdered Ben Parker (Supercharger was the first Spider-Man supervillain chronologically). He is also well known to be related to Kraven the Hunter. That revealed relationship helped evolve him as a major villain compared to his original depiction of being just a solo villain in the original issue of "The Amazing Spider-Man".
List of Spider-Man enemies
Most of the supervillains of Spider-Man would be introduced in "The Amazing Spider-Man" comic book starting with the Chameleon. The early villains would be introduced in the 1960s in the Silver Age of Comic Books, and created by Stan Lee and Steve Ditko. John Romita Sr. replaced Ditko starting with the Rhino. Gerry Conway later replaced Stan Lee and helped create new adversaries for the web-slinger and also helped pave the way to the Bronze Age of Comic Books with the death of Spider-Man's long time romantic interest, Gwen Stacy. Many collaborators would soon take over "The Amazing Spider-Man" title. One of the more popular examples included Todd McFarlane's Venom in the Modern Age of Comic Books.