Chameleon (comics)
Created by writer Stan Lee and artist Steve Ditko, the character first appeared in "The Amazing Spider-Man" #1 (March 1963), making him the first member of Spider-Man's rogues' gallery, based on issue publication date, excluding the burglar who murdered Ben Parker (Supercharger was the first Spider-Man supervillain chronologically). He is also well known to be related to Kraven the Hunter. That revealed relationship helped evolve him as a major villain compared to his original depiction of being just a solo villain in the original issue of "The Amazing Spider-Man".
Legends of the Superheroes
In the first episode, the Justice League of America (Batman, Robin, Flash, Green Lantern, Hawkman, Captain Marvel, Huntress and Black Canary) unite at the Hall of Heroes to celebrate elderly retired superhero Scarlet Cyclone's birthday. The party is interrupted by the Legion of Doom (Riddler, Weather Wizard, Sinestro, Mordru, Doctor Sivana, Giganta and Solomon Grundy), who announce they have hidden a deadly bomb in a secret location, and the heroes must follow clues to find it. It's all, actually, a trap to make the heroes drink Mordru's de-powering potion, which succeeds, but the heroes face off the villains even so, disarm the bomb and recover their powers. Only after the whole battle is gone, and the heroes have proven victorious, does Scarlet Cyclone arrive to give his aid.