Endangered Wolf Center
The Endangered Wolf Center is a non-profit wildlife facility in Eureka, Missouri, United States, near St. Louis that is dedicated to preserving and reintroducing to the wild critically endangered species of wolves. It is certified by the Association of Zoos and Aquariums (AZA). The Center was founded in 1971 by renowned naturalist Marlin Perkins and his wife Carol, who saw that wolves around the world were in danger of becoming extinct. Its mission is to preserve and protect Mexican wolves, red wolves, and other wild canid species, with purpose and passion, through carefully managed breeding, reintroduction and inspiring education programs. Animals born at the facility have been reintroduced to North Carolina (red wolves) and Arizona and New Mexico (Mexican wolves). The Center's research focuses on reproductive, behavioral and nutritional needs for the species housed there. The Endangered Wolf Center is a founding member of the AZA’s Mexican Wolf and Red Wolf Species Survival Plans. As of August 2016, the Center had six species of endangered canids: Mexican wolves, red wolves, maned wolves, African painted dogs, fennec foxes and swift foxes. It had more than 20 Mexican gray wolves, more than any other managed breeding facility. (Only 97 Mexican wolves were known to exist in the wild as of 2015.) The Center serves as the cornerstone of the U.S. Fish and Wildlife Service's program to preserve Mexican wolves and reintroduce them to the wild.
Red wolf
In 2000, a study looked at red wolves and eastern Canadian wolves. The study agreed that these two wolves readily hybridize with the coyote. The study used 8 microsatellites (genetic markers taken from across the genome of a specimen). The phylogenetic tree produced from the genetic sequences showed red wolves and eastern Canadian wolves clustering together. These then clustered next closer with the coyote and away from the gray wolf. A further analysis using mDNA sequences indicated the presence of coyote in both of these two wolves, and that these two wolves had diverged from the coyote 150,000–300,000 years ago. No gray wolf sequences were detected in the samples. The study proposes that these findings are inconsistent with the two wolves being subspecies of the gray wolf, that red wolves and eastern Canadian wolves evolved in North America after having diverged from the coyote, and therefore they are more likely to hybridize with coyotes. In 2009, a study of eastern Canadian wolves using microsatellites, mDNA, and the paternally-inherited yDNA markers found that the eastern Canadian wolf was a unique ecotype of the gray wolf that had undergone recent hybridization with other gray wolves and coyotes. It could find no evidence to support the findings of the earlier 2000 study regarding the eastern Canadian wolf. The study did not include the red wolf.