Dietary supplement
According to US & Canadian Dietary Reference Intake guidelines, the protein Recommended Dietary Allowance (RDA) for adults is based on 0.8 grams protein per kilogram body weight. The recommendation is for sedentary and lightly active people. Scientific reviews can conclude that a high protein diet, when combined with exercise, will increase muscle mass and strength, or conclude the opposite. The International Olympic Committee recommends protein intake targets for both strength and endurance athletes at about 1.2-1.8 g/kg body mass per day. One review proposed a maximum daily protein intake of approximately 25% of energy requirements, i.e., approximately 2.0 to 2.5 g/kg.
Puppy nutrition
Growing puppies require higher levels of protein than adult dogs of the same breed to promote proper growth and development. Protein should account for at least 25% of energy; however protein requirements also depend on the digestibility of the protein and age of the puppy. Amino acid and nitrogen requirements decrease between 10–14 weeks, indicating that different protein levels are often beneficial before and after 14 weeks of age. Before 14 weeks of age, a protein level of 250 g/kg of diet (25% of the diet) containing 4.0 kcal ME/g will result in optimal growth. After 14 weeks of age, protein requirements decrease to 200 g/kg of diet (20% of the diet) for optimal growth.