Lisbon Regicide
The assassination of King Carlos and the Prince Royal was the effective end of a constitutional monarchy in Portugal (later confirmed by the 5 October 1910 revolution). The regime functioned for another 33 months with growing agitation and demands for reform (although considerably less than in the future First Republic). It cannot be denied that the weak and permissive attitude in the Government of Acclamation was an incentive for the Republican Party to attempt another coup. The assassinations did not change the system of government; instead, they delayed the change.
Manuel, Hereditary Prince of Portugal
After the death of Henry, King of Portugal, his family's return to Portugal and his father's acclamation as King on 24 July 1580, Manuel chose to go by the title of Prince of Portugal. However, his father's reign was short and troubled. He lost his title to King Philip II of Spain at the Battle of Alcântra. After hearing about this defeat, Manuel and his father fled to Coimbra to avoid imprisonment, and gathered six thousand men to face the Spanish troops once again. Nevertheless, they were once more defeated. With no options left, Manuel and his father were forced to hide in monasteries and friends' houses until they fled to France with the rest of the family. Before, António went to England, asking for Queen Elizabeth's support, which was granted, but the English troops never arrived to Portugal due to a bout of plague that killed most of the soldiers. Despite everything, António was still acclaimed as King in Azores until the Spanish troops arrived in 1583.