Chameleon (comics)
Created by writer Stan Lee and artist Steve Ditko, the character first appeared in "The Amazing Spider-Man" #1 (March 1963), making him the first member of Spider-Man's rogues' gallery, based on issue publication date, excluding the burglar who murdered Ben Parker (Supercharger was the first Spider-Man supervillain chronologically). He is also well known to be related to Kraven the Hunter. That revealed relationship helped evolve him as a major villain compared to his original depiction of being just a solo villain in the original issue of "The Amazing Spider-Man".
Ezekiel (comics)
Recently, in "Grim Hunt", it appeared as if a reanimated Ezekiel (covered in spiders) appeared before Spider-Man and Arachne. Unknown to Spider-Man, the Ezekiel that appeared before him was really the Chameleon in disguise leading Spider-Man into a trap with the Kravinoffs in order to carry out the resurrection of Sergei Kravinoff, which required the blood of Spider-Man.