We introduce a method for comparing protein structures using the notion of residue contexts based on protein C alpha -atom backbones. The residue context is derived from the set of vectors from a given C alpha -atom to each other C alpha -atom in the molecule. A three-dimensional histogram is generated from these vectors, containing a relative distribution of the other C alpha -atoms for each C alpha -atom on the backbone for a protein. Histograms are compared using the chi 2  test, resulting in the cost for matching any two given C alpha -atoms in a pair of protein molecules. An optimal alignment is made using the Smith-Waterman algorithm, and a score is calculated based on the length of the alignment and the RMSD, yielding a best alignment that can be displayed in an interactive user interface. Resulting alignments are compared with alignments generated by CTSS, DALI, and CE, yielding different aligned protein regions.
['Tobias Sayre', 'Rahul Singh']
Protein Structure Comparison and Alignment Using Residue Contexts