Background#R##N#Technical advances in Next Generation Sequencing (NGS) provide a means to acquire deeper insights into cellular functions. The lack of standardized and automated methodologies poses a challenge for the analysis and interpretation of RNA sequencing data. We critically compare and evaluate state-of-the-art bioinformatics approaches and present a workflow that integrates the best performing data analysis, data evaluation and annotation methods in a Transparent, Reproducible and Automated PipeLINE (TRAPLINE) for RNA sequencing data processing (suitable for Illumina, SOLiD and Solexa).
['Markus Wolfien', 'Christian Rimmbach', 'Ulf Schmitz', 'Julia Jeannine Jung', 'Stefan Krebs', 'Gustav Steinhoff', 'Robert David', 'Olaf Wolkenhauer']
TRAPLINE: a standardized and automated pipeline for RNA sequencing data analysis, evaluation and annotation.