Background#R##N#The number of γH2AX foci per nucleus is an accepted measure of the number of DNA double-strand breaks in single cells. One of the experimental techniques for γH2AX detection in cultured cells is immunofluorescent labelling of γH2AX and nuclei followed by microscopy imaging and analysis.
['Anastasiya Lapytsko', 'Gabriel Kollarovic', 'Lyubomira Ivanova', 'Maja Studencka', 'Jörg Schaber']
FoCo: a simple and robust quantification algorithm of nuclear foci