Summary: DiffTool is a resource to build and visualize protein clusters computed from a sequence database. The package provides a clustering tool to construct protein families according to sequence similarities and a web interface to query the corresponding clusters. A subtractive genome analysis tool selects protein families specific for a genome or a group of genomes. For each protein cluster, DiffTool includes access to sequences, coloured multiple alignments and phylogenetic trees. Availability: A cluster database built from yeast and complete prokaryotic genomes is queryable at http:// bioweb.pasteur.fr/seqanal/difftool. All the Perl sources are freely available to non-profit organizations upon request.
['Farid Chetouani', 'Philippe Glaser', 'Frank Kunst']
DiffTool: building, visualizing and querying protein clusters