For developing new stem cell separation tool, we have prepared thermoresponsive cationic copolymer brush, poly(N-isopropylacrylamide-co-N,N-dimethylaminopropylacrylamide-co-N-tert-butylacrylamide (IPAAm-co-DMAPAAm-co-tBAAm) on glass substrates through surface-initiated atom transfer radical copolymerization (ATRP). Using the prepared thermoresponsive cationic copolymer brush, mesenchymal stem cell separation was performed simply by changing temperature.
['Kenichi Nagase', 'Yuri Hatakeyama', 'Tatsuya Shimizu', 'Katsuhisa Matsuura', 'Masayuki Yamato', 'Naoya Takeda', 'Teruo Okano']
Stem cell separation using thermoresponsive copolymer brushes having cationic charge