Replication data for: A novel sweet potato potyvirus open reading frame (ORF) is expressed via polymerase slippage and suppresses RNA silencing
<|begin_of_text|><|start_header_id|>system<|end_header_id|> You are a research archivist expert in dataset metadata. Answer the question truthfully.  <|eot_id|><|start_header_id|>user<|end_header_id|> Here is the Description of a dataset. Description:'The accumulation of GFP mRNA and GFP mRNA-derived siRNA was evaluated by northern blot analysis in leaf tissues agroinfiltrated for coexpression of GFP and P1, P1N-PISPO, SPFMV HCpro, SPLV P1, PVA HCpro or GUS. Total RNA was extracted with TRIzol LS Reagent (Invitrogen) according to the manufacturer’s recommendations alow-molecular-weight (LMW) and high-molecular-weight (HMW) RNA fractions were separated as described in Cuellar et al. (2009). LMW RNA (15 lg/lane) and HMW RNA (10 lg/lane) were separated by electrophoresis in a denaturing 15% polyacrylamide gel and 1.2% (w/v) agarose gel, respectively, both containing formaldehyde. The two gels were transferred to a nylon membrane (GE Healthcare) and crosslinked chemically with 1-ethyl-3-(3-dimethylaminopropyl)carbodi-imide (Pall and Hamilton, 2008) or by UV light (Sambrook and Russell, 2' . Please return only a json list of at most 3 Keywords corresponding to the Description:.<|eot_id|><|start_header_id|>assistant<|end_header_id|> {"Answer" : ["Frameshift","Gene expression","Overlapping gene","RNA silencing suppression","RNA virus","Transcriptional slippage"]} <|eot_id|>
 Here is the Description of a dataset. Description:'The accumulation of GFP mRNA and GFP mRNA-derived siRNA was evaluated by northern blot analysis in leaf tissues agroinfiltrated for coexpression of GFP and P1, P1N-PISPO, SPFMV HCpro, SPLV P1, PVA HCpro or GUS. Total RNA was extracted with TRIzol LS Reagent (Invitrogen) according to the manufacturer’s recommendations alow-molecular-weight (LMW) and high-molecular-weight (HMW) RNA fractions were separated as described in Cuellar et al. (2009). LMW RNA (15 lg/lane) and HMW RNA (10 lg/lane) were separated by electrophoresis in a denaturing 15% polyacrylamide gel and 1.2% (w/v) agarose gel, respectively, both containing formaldehyde. The two gels were transferred to a nylon membrane (GE Healthcare) and crosslinked chemically with 1-ethyl-3-(3-dimethylaminopropyl)carbodi-imide (Pall and Hamilton, 2008) or by UV light (Sambrook and Russell, 2' . Please return only a json list of at most 3 Keywords corresponding to the Description:.
The accumulation of GFP mRNA and GFP mRNA-derived siRNA was evaluated by northern blot analysis in leaf tissues agroinfiltrated for coexpression of GFP and P1, P1N-PISPO, SPFMV HCpro, SPLV P1, PVA HCpro or GUS. Total RNA was extracted with TRIzol LS Reagent (Invitrogen) according to the manufacturer’s recommendations alow-molecular-weight (LMW) and high-molecular-weight (HMW) RNA fractions were separated as described in Cuellar et al. (2009). LMW RNA (15 lg/lane) and HMW RNA (10 lg/lane) were separated by electrophoresis in a denaturing 15% polyacrylamide gel and 1.2% (w/v) agarose gel, respectively, both containing formaldehyde. The two gels were transferred to a nylon membrane (GE Healthcare) and crosslinked chemically with 1-ethyl-3-(3-dimethylaminopropyl)carbodi-imide (Pall and Hamilton, 2008) or by UV light (Sambrook and Russell, 2
Frameshift; Gene expression; Overlapping gene; RNA silencing suppression; RNA virus; Transcriptional slippage