Multi-modal on-chip nanoscopy and quantitative phase imaging reveals the nanoscale morphology of liver sinusoidal endothelial cells
<|begin_of_text|><|start_header_id|>system<|end_header_id|> You are a research archivist expert in dataset metadata. Answer the question truthfully.  <|eot_id|><|start_header_id|>user<|end_header_id|> Here is the Description of a dataset. Description:'Visualization of 3D morphological changes in the subcellular structures of a biological specimen is a major challenge in life science. Despite conspicuous refinements in optical nanoscopy, the determination of quantitative changes in subcellular structures, i.e., size and thickness, remains elusive. We present an integrated chip-based optical nanoscopy set-up that provides a lateral optical resolution of 61 nm, combined with a quantitative phase microscopy (QPM) system with a spatial phase sensitivity of ±20 mrad. We used the system to obtain 3D morphology of liver sinusoidal endothelial cells (LSEC) by using chip-based nanoscopy for lateral super-resolution, and high spatially sensitive QPM for nanoscale axial sensitivity. LSEC have unique morphology with small nanopores (50-300 nm in diameter) in the plasma membrane, called fenestrati' . Please return only a json list of at most 3 Keywords corresponding to the Description:.<|eot_id|><|start_header_id|>assistant<|end_header_id|> {"Answer" : ["QPI","quantitative phase imaging, single molecule localization microscopy, liver sinusoidal endothelial cells"]} <|eot_id|>
 Here is the Description of a dataset. Description:'Visualization of 3D morphological changes in the subcellular structures of a biological specimen is a major challenge in life science. Despite conspicuous refinements in optical nanoscopy, the determination of quantitative changes in subcellular structures, i.e., size and thickness, remains elusive. We present an integrated chip-based optical nanoscopy set-up that provides a lateral optical resolution of 61 nm, combined with a quantitative phase microscopy (QPM) system with a spatial phase sensitivity of ±20 mrad. We used the system to obtain 3D morphology of liver sinusoidal endothelial cells (LSEC) by using chip-based nanoscopy for lateral super-resolution, and high spatially sensitive QPM for nanoscale axial sensitivity. LSEC have unique morphology with small nanopores (50-300 nm in diameter) in the plasma membrane, called fenestrati' . Please return only a json list of at most 3 Keywords corresponding to the Description:.
Visualization of 3D morphological changes in the subcellular structures of a biological specimen is a major challenge in life science. Despite conspicuous refinements in optical nanoscopy, the determination of quantitative changes in subcellular structures, i.e., size and thickness, remains elusive. We present an integrated chip-based optical nanoscopy set-up that provides a lateral optical resolution of 61 nm, combined with a quantitative phase microscopy (QPM) system with a spatial phase sensitivity of ±20 mrad. We used the system to obtain 3D morphology of liver sinusoidal endothelial cells (LSEC) by using chip-based nanoscopy for lateral super-resolution, and high spatially sensitive QPM for nanoscale axial sensitivity. LSEC have unique morphology with small nanopores (50-300 nm in diameter) in the plasma membrane, called fenestrati
QPI; quantitative phase imaging, single molecule localization microscopy, liver sinusoidal endothelial cells