SEM showed that the three dimensional hierrarchial arrangement of the matrix was maintained at day 14 for protocol B. Protocol A was not ideal since it not only necessitated 35 days to decellularize the cartilage but in doing so it obliterated the ECM’s three dimensional (3D) structure. Surface characterization of cartilage post decellularization with SEM has also been reported by Utomo15 as well as Gomez18 and is an effective method of ECM analysis to asses for gross changes in the scaffold’s structure.