The difference between CalpTG and WT mice in skin phenotype was evident at two years (Fig. 6A,B). There were less lipofuscine deposits, a classical feature of skin aging, in CalpTG mice at 2 years than in WT animals (Fig. 6C–F). In addition, telomere shortening with aging was significant in WT mice but not in CalpTG mice (Fig. 6G, p = 0.0047).Figure 6Calpastatin overexpression protects against skin aging. At 2 years, CalpTG (TG) mice (B) were phenotypically less impacted by aging than WT mice bred in the same conditions (A). There was a macroscopic evidence that CalpTG (TG) mice fur was less affected by aging than WT mice. In addition, we observed less lipofuscin deposits in the skin after hematoxylin eosin staining (C,D, white arrow, magnification ×200) or by using lipofuscine autofluorescent properties (E,F, white arrow, magnification ×200). Moreover, telomere lenght decreased significantly in WT mice skin but not in CalpTG skin (G, **p < 0.01, n = 10/group).