Application of next generation sequencing (NGS) technology in small RNA profiling has significantly impact on the studies of plant-virus interactions. Li et al. investigate profiles of the Cucumber green mottle mosaic virus (CGMMV)-derived siRNAs (vsiRNA) in infected leaves and fruits of L. siceraria using NGS. The vsiRNA patterns of abundance, origination and polarity, hotspot distribution, GC content and 5′ terminal nucleotide are compared between the infected leaves and fruits. The similarities and distinct differences are revealed by this analysis. Co-infection of none-coding satellite RNAs (sat-RNAs) usually inhibits replication and attenuates disease symptoms of helper viruses. However, Xu et al. reveal that co-infection of none-coding satellite RNAs (sat-RNAs) of Beet black scorch virus (BBSV) enhances the replication and the symptoms of BBSV on N. benthamiana possibly through competitively occupying or saturating host silencing machinery. Fang et al. narrow down the functional domains of 2b protein of Cucumber mosaic virus (CMV) for dsRNA binding and Argonaute (AGO) interaction. Their findings demonstrate that the dsRNA-binding activity of the 2b is essential for virulence, whereas the 2b-AGO interaction is necessary for interference with RDR1/6-dependent antiviral silencing in Arabidopsis. Alpha-momorcharin (α-MMC) is a type-I ribosome inactivating protein (RIP) in Momordica charantia. Yang et al. provide evidence that α-MMC plays a positive role in the resistance against CMV in M. charantia and the antiviral activities of α-MMC may be achieved through up-regulating JA and ROS signaling pathway.