To examine whether supaglutide attenuates obesity, HFD-induced obese mice (body weight >50 g) were randomized grouped and injected without (control) or with supaglutide (300 μg/kg) twice a week; the body weights of the mice were measured at the day of injection. Under HFD feeding conditions, while untreated obese mice continue to yield their body weight, supaglutide treatment significantly decreased their body weight in obese mice (Figure 2A). The weight-sparing effects were observed 3 days after the first injection, and the supaglutide-treated mice kept leaner throughout the experiment.