pFlag-ErbB2, pcDNA3-ErbB3, pFlag-ErbB4 and pcDNA3-EGFR were used in previous reports [77–79]. DnEGFR complementary DNA was amplified from genomic DNA of TRE-dnEGFR mice and cloned into pcDNA3.1/myc-His (−)A vector by using cloning sites BamHI and XhoI. Constructed plasmid was purified and sequenced. Fused myc-tagged dnEGFR was verified by detecting a band around 105 kDa in lysates of transfected HEK293 cells using anti-myc antibody (9E10) for WB.