OTUs were binned into taxonomic groupings allowing comparison of fungal diversity within the GI tract of wood-fed fish and mixed-fed fish, irrespective of GI tract region. The GI tract of the mixed-fed fish was found to have the most ITS2 region sequence diversity. Chao1 analysis indicated that the wood-fed fish had a higher number of rare and unique species compared to mixed-diet fed fish (Table 2). The most prevalent classes in the wood-diet fed fish were sequences similar to Dothideomycetes (40%) and Sordariomycetes (36%) and the mixed-diet fed fish were sequences similar to Sordariomycetes (73%). Three OTUs detected from the Dothideomycetes class were found in the foregut, midgut and hindgut of the wood-diet fed fish but absent in the mixed-diet fed fish.