By contrast, when maintained on RC, female mice (Fig. 5) displayed abnormal IPGTT at 12 wk of age (Fig. 5C). This resolved at 16 wk but was again apparent at 20 wk (Fig. 5G). Consistent with observations in males (Fig. 4), female βVps13cKO mice fed HFD similarly failed to show abnormalities in IPGTT up to 20 wk of age (Fig. 5, B, D, F, H).