Buffalo milk was obtained from farmers in Kizilirmak Delta in Bafra, Turkey. Plain starter cultures were obtained from Peyma Chr. Hansen, Istanbul, Turkey. Freeze‐dried (FD‐DVS) plain starter culture (YC‐X11) consisting of S. thermophilus and L. delbrueckii subsp. bulgaricus and probiotic starter cultures (ABY‐2 and ABT‐4) consisting of Bifidobacterium BB‐12®, L. acidophilus LA‐5®, S. thermophilus, and L. delbrueckii subsp. bulgaricus, and Bifidobacterium BB‐12®, L. acidophilus LA‐5®, and S. thermophilus, respectively, were obtained from Peyma Chr. Hansen, Istanbul, Turkey.