Next, HVEM(23–39) was analyzed in an competitive ELISA test, in order to determine whether this fragment may bind to BTLA and inhibit BTLA/HVEM interaction. Fig 3 shows that the HVEM(23–39) can partially prevent the ligation of HVEM to BTLA, in a dose dependent manner, while scrambled peptide (Ac-ELCAGPVTRKVECTYGE-NH2) has no effect. Moreover, the scrambled peptide (named Ctrl peptide in figures) did not interact with BTLA immobilized in microcolumn (Table 1).