Approximately 900 bp fragments of the 28S rDNA (for 105 specimens) and the mitochondrial COI gene (for 399 specimens) were PCR amplified using the Qiagen Multiplex PCR kit according to the manufacturer's protocol and using the primers 28Srd4.8a and 28Srd7b1 (Schwendinger and Giribet 2005) and C1‐J‐1718‐spider and C1‐N‐2776‐spider (Vink et al. 2005). While COI is well known as a useful phylogeographic and taxonomic marker in spiders (Krehenwinkel and Tautz 2013), the more slowly evolving 28S might also be suited to uncover interspecific divergence in spiders (Jäger et al. 2015). We thus use it as a backup for the COI data here. Sequence traces were edited using Codon Code Aligner (Codon Code Corporation, Centerville, MA, USA) and aligned in MEGA 6 (Tamura et al. 2013) under default alignment parameters. The mitochondrial sequences were translated to amino acids to detect potential presence of pseudogenes.