Rat monoclonal antibody to ankyrin-G clone N106/36, NeuroMab Davis, CA, USA, diluted 1:1000; goat polyclonal antibody to doublecortin (Santa Cruz Biotechnology, Inc., CA, USA; diluted1:200; mouse monoclonal antibody to BrdU, Roche Diagnostic, Lewes, UK, diluted 1:1000; mouse monoclonal antibody to IGF-1R, clone ab80547, Abcam, Cambridge, MA, USA; diluted 1/100.