In order to confirm and better understand the relationship between sequence differences and nAMPs, GenMark Diagnostics provided single-stranded DNA matching the capture probe sequences of the H3N2 and H1N1 strains. When this synthetic DNA was run in the eSensor, equimolar concentrations gave average nAMPs of 150.5 ± 17.2 and 72.9 ± 16.3 nAMPs (N = 4, p = 0.0006, t-test), for the sequences matching the H1N1 strain and H3N2 strains, respectively (see Table 2). A capture probe with a perfect sequence match to the GenMark assay was tested at GenMark and gave the same average nAMPs as the H1N1 sequence, despite the 2 mismatches in the H1N1 sequence (data not shown).