Integrated approaches provide a better perspective for developing a specific biosensor designed to catalyze the production and/or degradation of the desired compound. To achieve this, it is necessary to rewire the genetic circuits of bacteria using the above synthetic devices. Design of the engineered system should be based on strategies for building sensory regulation components that incorporate a target substrate-responsive TCRS in any desired host (Fig. 4). Introducing a sensory regulation device in a host cell enables it to sense the target compound and trigger the genetic circuit, achieving real-time monitoring of the compound present and upregulation of the effector protein’s gene expression. Use of engineered TCRSs in bacteria would prevent the production of redundant proteins at the initial growth phase and avoid the use of toxic and costly chemical inducers.Fig. 4Synthetic TCRS with integrated biosensing and bioremediating functions for the detection of the target compound and upregulation of the effector protein that allow real-time detection of controlled gene expression