Despite presenting higher levels of TSDR demethylation and Foxp3 expression, expanded naive Treg cells were not significantly more suppressive than CD25int mTreg cells (Figure 4A); suppression was similar between total Treg cells, CD25int mTreg cells, and naive Tres cells after expansion in DMSO control media (Figure 4A). This indicates that those CD25int mTreg cells that express FOXP3 but do not retain a demethylated TSDR are iTreg cells that are capable of suppressing.