Cas5 regulates caspofungin tolerance in part through the interaction with components of the SBF complex, Swi4 and Swi6. a Swi4 and Swi6 co-purify with Cas5. C terminally HA-tagged Cas5 was immunoprecipitated with α-HA beads. Swi4 and Swi6 co-purification was monitored by western blot and detected with an α-TAP antibody. Cas5 pull down was confirmed by detection with an α-HA antibody. Input samples confirm the expression of tagged proteins. Full blots are shown in Supplementary Fig. 6. b Cas5, Swi4, and Swi6 are required for the upregulation of caspofungin-responsive genes. The transcript levels of ECM331 and PGA13 was monitored by qRT-PCR and normalized to GPD1. Plotted are the fold-changes in transcript levels in the presence of caspofungin relative to untreated. Error bars represent standard deviation (s.d.) from the mean of triplicate samples. The fold change in gene expression for each mutant strain was compared to the wildtype using one-way ANOVA in GraphPad Prism (****P < 0.0001). c Swi4 and Swi6 regulate cell wall stress response. Caspofungin susceptibility assays were conducted in YPD medium. Growth was measured by absorbance at 600 nm after 48 h at 30 °C. Optical densities were averaged for duplicate measurements. Data was quantitatively displayed with color using Treeview (see color bar in Fig. 2)