Chromosomes of Phasmatodea were up to now mainly studied by routine staining approaches (i.e., Giemsa). Even centromere banding staining (C-banding) of stick insect chromosomes was described only for a few species . Molecular cytogenetics, i.e., fluorescence in situ hybridization (FISH) in stick insects, was applied in a single study . Thus, here we intended to provide karyotypic characterization of the following five species from four families applying C-banding, and to characterize the distribution of 18S rDNA and telomeric repeats by FISH: