Human MG-63 osteoblast-like cells (KCLB, Seoul, Korea) were used for cell attachment and proliferation test. They were cultured at 37 °C in a humidified 5 % CO2 and cultured in DMEM containing 10 % fetal bovine serum and 1 % penicillin/streptomycin. Cells were sub-cultured every 3 days at 90 % confluence in 75 cm2 and 25 cm2 culture flask. 4~6 passage cells were used for this study. The cellular experiments were performed in 24-well plate and 12-well plate where each well was immersed by the culture medium.