Chinese mitten crab (Eriocheir sinensis) is one of the most important aquaculture species, which is widely distributed in freshwater and low-salinity estuarine regions in China [1, 2]. Its culture under facility conditions started in the early 1980s and the annual output has increased during the past decade . In 2014, the domestic total production was more than 750,000 tons for aquaculture only. The male mitten crabs grow faster and are more aggressive than females in the current traditional culture system, which may result in high mortality of the females and thus influence the production of the crab. It is obvious that this culture of all-male or all-female populations would be more economically advantageous to crab aquaculture industry [4, 5].