For antagomir experiments, miRCURY LNA Power Inhibitors (Exiqon #4101004-100, #4103258-100, and #199006-100) were transfected into 2.5 × 105 cells per well of a 6-well plate using Superfect (Qiagen #301305) according to manufacturer's specifications at a final antagomir concentration of either 50 or 100 nM.