Rabbit anti-human phospho-AKT (Thr308), rabbit anti-human total-AKT, rabbit anti-human p53, rabbit anti-human c-Myc, horseradish peroxidase-conjugated sheep anti-rabbit IgG antibodies were from Cell Signaling Technology (Cell Signaling Technology, Danvers, MA, USA). Mouse anti-human glyceraldehydes 3-phosphate dehydrogenase (GAPDH) was purchased from Bio-tech (Kangchen Bio-tech, Shanghai, China). Rabbit anti-human 14-3-3, rabbit anti-human phospho-14-3-3 β + ζ (Ser184 + Ser186), rabbit anti-human AKAP8, rabbit anti-human total-BID, rabbit anti-human phospho-BID (Ser61), rabbit anti-human BAX, rabbit anti-human BCL-2, rabbit anti-human adenyl cyclase 4 (AC4) were purchased from Abcam (Cambridge, UK).