In this study, the effectiveness of different activation protocols of irrigation solutions were compared between themselves and with traditional irrigation methods. The results are consistent with the findings of the above-mentioned authors. According to our results, LAI and PUI were more effective in the elimination of CH in all 3 regions of the root canal. Therefore, the null hypothesis  that no differences would occur among the different irrigation techniques in terms of CH removal was rejected. In addition, no difference was found between PUI and XP-endo Finisher groups in the coronal and middle third regions. Although CanalBrush and sonic-activated irrigation exhibited lower scores than needle irrigation groups, the difference was not statistically significant. Results of our study indicate that there was a significant difference between sections of the root canal in terms of CH removal except for the CanalBrush group; therefore, the second null hypothesis is also rejected.