For protocol A at day 35 there was a 0.22 fold reduction in collagen after decellularization compared to native which was not significant (P-value: 0.23). The same was the case for protocol C at day 14 where there was a comparable reduction of only 0.2 fold (P-value: 0.23). In protocol B at day 14 there was an increase in collagen content compared to native of 20% from 333 μg/ml to 400 μg/ml however this was insignificant (P-value: 0.37). There was no change in collagen content between day 7 and 14 of protocol B with mean values of 400 μg/ml at both time points. For protocol C the average collagen content decreased from 300 μg/ml at day 7 to 267 μg/ml at day 14 which marked a 0.11 fold reduction however this was not considered significant. Comparison of the collagen content for native cartilage, day 35 of protocol A, day 14 of protocol B and C showed no significant difference at these time points (P-value: 0.076). This has been reflected by histology below where there was a similar density of collagen stained with masons trichrome amongst the different protocols (Fig. 6).Figure 6Masson’s trichrome stain of elastic cartilage. Same density distribution of collagen fibres between lacunae amongst different protocol groups. (A) Native. (B) Protocol A, day 28. (C) Protocol B, day 7. (D) Protocol B, day 14. (E) Protocol C, day 7. (F) Protocol C, day 14.