Western blot analysis was performed to validate the identity of translationally controlled tumor protein and ATP-specific succinyl-CoA synthetase beta subunit as differentially expressed hippocampal proteins and the identity of gamma-enolase isoform 1 as differentially expressed cortical protein. The protein level of translationally controlled tumor protein was significantly decreased about 0.8-fold in the hippocampus of STZ-treated mice compared with untreated control (P = 0.049) (Figure 3(a)). The protein level of hippocampal ATP-specific succinyl-CoA synthetase beta subunit tended to increase (P = 0.29) (Figure 3(b)) and that of cortical gamma-enolase isoform 1 tended to decrease (P = 0.057) (Figure 3(c)).