10.7554/eLife.24635.003Figure 1—figure supplement 1.pZwitch vector.(A) Schematic of pZwitch. (B) Sequence of the splice acceptor and P2A components of pZwitch (filled bar in A). The splice acceptor sequence was derived from pFT1 (Ni et al., 2012). pZwitch for the +2 and+3 reading frames was generated by inserting T and TCGAT, respectively, at the indicated site. BP, branch point; ESE, exonic splice enhancer; ISE, intronic splice enhancer; SAC, splice acceptor consensus.DOI: http://dx.doi.org/10.7554/eLife.24635.003