In the present study, 67 male adolescents (14–18 years) who played online games were recruited. The voxel-based correlation analysis was conducted to detect the brain regions associated with IAT score before and after controlling for the total time of playing online game. Based on the previous studies, the prefrontal-striatal circuits are closely related to the addiction. Ventral striatum participated in the habit learning and rewarding process involved in addiction (26, 27), and the reduced control effect of prefrontal cortex on rewarding process is one of the mechanisms of addiction (28, 29). Therefore, we hypothesized that the IGD tendency may be associated with the brain regions related to the cognitive control (prefrontal cortex) and the rewarding process (ventral striatum). This study may lead to new targets for preventing and treating the IGD in adolescents.