Next, we assessed if hypoxia could induce KDM2A and KDM2B protein levels. Western blot analysis revealed that, indeed, hypoxia induces KDM2A protein (Figure 2A) in the cell lines analysed. While numerous attempts were made to investigate KDM2B protein levels by Western blot, none of the antibodies tested were specific for KDM2B when an siRNA depletion control was included (Figure S1A), despite all the antibodies presenting a band of the expected size (Figure S1B–D). However, we could determine that one particular antibody was specific when used in immunofluorescence, accessed by siRNA depletion, overexpression of KDM2B, and specific controls for antibody staining (Figure S2A–C).