RNA samples from kidney cortex coming from either old C57BL6J (n = 6) or Calpastatin transgenic (CalpTG) mice (n = 6) of high quality (RNA Integrity Number >6) were processed to create mRNA libraries using TrueSeq reagents (Illumina, san diego, California). Clusters were created for Illumina RNA-Sequencing using a CBot system and sequencing was carried out on 2 lanes of HiSeq. 2000 to produce paired-end reads (75 bases length, 29 to 35 millions of reads per sample) and organized per lane to avoid sequencing batch effects. After demultiplexing, bare-coding removal, quality control, paired-end reads were processed from Fastq Sanger format to compact-reads format and then align with STAR (default parameters) to the mouse reference genome (mGC37/mm9). Data alignment and analysis were performed using the GobyWeb interface using Ensembl version 55. Differential expression was computed using the EdgeR package from bioconductor. Data were also examined visually in the Integrative Genomic Viewer (http://www.broadinstitute.org/igv/) respective of Ensembl Annotation (Ensembl version 55) (Anders et al., 2013; Dorff et al., 2013; Robinson et al., 2010). Normalized gene expression level was measured by counts per millions (CPM). Genes that were significantly differentially expressed (DE) between groups (WT vs. CalpT q < 0.05, Edge R P-value adjusted for multiple testing with the Benjamini Hochberg approach) were next selected by their Fold change |LogFC| > 1.2 to build heatmap (R package Pheatmap) and pathway using David 6.8 available at https://david.ncifcrf.gov/home.jsp.