The oral cavity is a suitable milieu for bacterial growth and propagation. The presence of bacteria in the mouth readily stimulates the formation of dental plaque, which accumulates on both hard and soft tissues as dental calculus. Although the regional colonization and invasion of bacteria are rigorously controlled by the dynamic equilibrium between dental plaque bacteria (DPB) and the host’s innate defense mechanisms , plaque that extends subgingivally can trigger the immune system imbalance, inducing an inflammatory response . Gingivitis and periodontitis are the most common plaque-induced inflammatory conditions. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, and Tannerella forsythensis are the most prevalent anaerobic gram-negative bacteria in subgingival area. All are critical in the onset and subsequent development of periodontitis. If untreated, these bacteria can lead to the periodontal pocket, connective tissue destruction, and alveolar bone resorption .