Brains were cut into 10 µm coronal sections after spotting of hypothalamus. Sections were rehydrated and then heated in pH6 citric acid bath. After blocking and permeabilization with PBS BSA 2% and Triton 0.1%, astrocytes were marked with a rabbit anti-GFAP polyclonal antibody (ab7260, Abcam, 1/2000), and secondary antibody Alexa Fluor 488 goat anti rabbit (A11008, Invitrogen, 1/1000). Nucleus was marked with DAPI (Invitrogen, Thermofischer scientific, 1/2000). Astrocytic process measures were performed on 6 stacks of images of hippocampus CA1 region for each mouse. Each stack was obtained by addition of photos at 600x magnification by using an Olympus ix83 microscope and CellSens Dimension software. Astrocytes morphometry was carried out with Image J software, using the plugin NeuronJ. The mean length of astrocytes processes was calculated for each mouse.