As a reference for other researchers, we report in Table 3 a detailed ampelographic description of the five varieties under study. From the entire ampelographic and ampelometric data set it was possible to perform an analysis of similarity between each parent and its offspring: the software SuperAmpelo (as described in the user manual (http://www.pomologia.it/SuperAmpelo/Documenti.aspx) calculated that the Bombino nero has the highest similarity index (63 %) with the parent Uva rosa antica/Quagliano while the Impigno and Uva di Troia show, respectively, a similarity of 70 and 65 % with the parent Bombino Bianco (Fig. 2). No other significant morphological similarity were observed between the parents nor the offspring. In spite of very demanding analyzes and generating massive ampelographic and ampelometric data set (in contrary to microsatellites), it was not sufficient to help or contribute revealing of proposed kinship. Table 3Ampelographic characters: in Uva rosa antica/Quagliano, Bombino bianco, Uva di Troia, Bombino nero and Impigno Caracter code and description Uva rosa antica/Quagliano Bombino bianco Uva di Troia Bombino nero Impigno 001 Young shoot: opening of the shoot tip55555003 Young shoot: intensity of anthocyanin coloration on prostrate hairs of the shoot tip37151004 Young shoot: density of prostrate hairs on the shoot tip15155006 Shoot: attitude (before tying)33317007 Shoot: color of the dorsal side of internodes32332008 Shoot: color of the ventral side of internodes22223016 Shoot: number of consecutive tendrils11111051 Young leaf: color of upper side of blade (4th leaf)13111053 Young leaf: density of prostrate hairs between main veins on lower side of blade (4th leaf)15371067 Mature leaf: shape of blade33223068 Mature leaf: number of lobes33323070 Mature leaf: area of anthocyanin coloration of main veins on upper side of blade13313072 Mature leaf: goffering of blade11115074 Mature leaf: profile of blade in cross section21211075 Mature leaf: blistering of upper side of blade11151076 Mature leaf: shape of teeth43455079 Mature leaf: degree of opening/overlapping of petiole sinus55777080 Mature leaf: shape of base of petiole sinus21121081-1 Mature leaf: teeth in the petiole sinus19111081-2 Mature leaf: petiole sinus base limited by vein11111083-2 Mature leaf: teeth in the upper lateral sinuses19111084 Mature leaf: density of prostrate hairs between main veins on lower side of blade15573087 Mature leaf: density of erect hairs on main veins on lower side of blade75713094 Mature leaf: depth of upper lateral sinuses55555202 Bunch: length (peduncle excluded)57335204 Bunch: density73337206 Bunch: length of peduncle of primary bunch53133208 Bunch: shape13111209 Bunch: number of wings of the primary bunch23222220 Berry: length55555221 Berry: width55554223 Berry: shape22323225 Berry: color of skin51661231 Berry: intensity of flesh anthocyanin coloration11111Values are referred to the ‘Descriptor List for grape varieties and Vitis species’ of the Organisation Internationale de la Vigne et du VinFig. 2Leaves ampelometric comparison. Ampelometric comparison with the highest Similarity Index between the mean profiles of parental and offspring leaves (respectively Uva rosa antica/Quagliano vs Bombino nero, Bombino bianco vs Uva di Troia and Bombino bianco vs Impigno)