Briefly, an ethanol solution of lecithin 2.5% (w/v) containing 0.625% w/v of CP and 2% w/v of isopropyl myristate (IPM) was prepared. Nanoparticles were obtained by rapidly injecting 4 mL of the lecithin ethanol solution through a glass pipette (internal diameter 0.75 mm, injection rate 40 mL/min) under mechanic stirring (Ultraturrax TP 18/10-10N, IKA Werke, Staufen, Germany), into 46 mL of a chitosan solution obtained by diluting with distilled water 0.5 mL of 1% (w/v) chitosan solution in 0.275 N HCl. In the colloidal suspension, lecithin/chitosan ratio was 20:1 (w/w) and the final CP content was 0.050% w/v.