For the transferability evaluation, the 15 primer pairs were first evaluated by PCR in 10 A. flaccida individuals. The amplification was performed in a final volume of 10 μL containing 1X PCR buffer, 3 mM MgCl2, 0.2 mM dNTP, 0.8 mM each of forward and reverse primers, 0.4 U Taq DNA Polymerase), 20 ng DNA, and ultrapure water (to complete the volume).