At 10 weeks of age, male mice were subcutaneously injected with 1 × 107 TGC/mouse once on day 0 and again on day 14 (i.e., at a 2-week interval) for induction of active EAO. Male mice (aging 10 weeks) injected with HBSS alone were used as controls. At 120 days after the first immunization, the mice were deeply anesthetized with pentobarbital (65 mg/kg body weight) and their testes were removed. Histopathological samples were taken from the right testes, and immunohistochemical samples were collected from the left testes of the mice (n = 10 for both the TGC-immunized mice and control mice). Blood samples were collected from the mice by cardiac puncture (n = 10 for each group).