Moreover, pretreatment with a 5-HT2A agonist reduced the acute anorexic effects of liraglutide. We previously reported that intraperitoneal injection of sarpogrelate hydrochloride, a blood-brain barrier-penetrating selective 5-HT2A receptor antagonist, acutely suppresses food intake and chronic administration of sarpogrelate hydrochloride decreases daily food intake and body weight in mice . These results suggest that GLP-1 receptors downregulate the expression of 5-HT2A receptors in the hypothalamus and decreased hypothalamic 5-HT2A receptor signaling might be involved in the acute anorexic effects of GLP-1 receptor agonists independently of brain 5-HT.