Standard molecular biology techniques were used for the construction of the pEGFP-C1-CsGRN recombinant plasmid. The PCR product of the CsGRN fragment was cut and inserted between the Xhol I (Thermo Scientific) and EcoR I (Thermo Scientific) restriction sites in the pEGFP-C1 vector (Promega). The forward and reverse primers (restriction sites are underlined) used to amplify this fragment included forward 5′-GCG CCT CGA GTG TAA ATA TAA CCA GAC-3′ (Xhol I) and reserve 5′-ATA AGG ATC CCG GAG CAC AGG TGT AG-3′ (EcoR I) respectively, based on the following conditions: 30 s denaturation at 94 °C, 30 s annealing at 60 °C, and 1 min extension at 72 °C for 30 cycles, plus 72 °C for 10 min. Cells (1 × 106) plated in a 6-well cell culture cluster were transfected with either 0.8 μg of pGFP-C1 or pGFP-C1-CsGRN using lipofectamine 2000 (Invitrogen, Carlsbad, USA) according to the manufacturer’s instructions. Two days after transfection, the stable cell line selection was started using the optimal concentration of G418. The medium was changed every 2–3 days, and the cells were split when necessary. After 2–4 weeks, all of the non-transfected cells disappeared, and isolated colonies began to appear. The selective overexpression of GFP or CsGRN cells was designated as PLC-GFP/RBE-GFP cells and PLC-GRN/RBE-GRN cells, respectively. CsGRN overexpression in these cells was checked by a fluorescence microscope, fluorescence activated cell sorting (FACS) analysis, qRT-PCR analysis and Western blotting first incubated with mouse anti-CsGRN sera (1:100 dilution).