Following the discontinuation of GnRHa-treatment at 44 weeks of age, the reproductive axis was no longer suppressed, as testes size increased to a similar size as untreated rams by 83 weeks of age and was larger at the time of the breeding versus non-breeding season. This provides evidence that endogenous GnRH and gonadal steroid signaling was restored. This delayed exposure to gonadal steroid signaling did not alter the speed at which animals completed the spatial tasks during assessments of spatial orientation (i.e. first maze attempt) and learning (progress over 3 same-day maze attempts) after peripubertal GnRHa-treatment had ceased, but affected the manner in how quickly rams moved beyond a specific point within the maze, over three same-day attempts. On assessment of long-term spatial memory at 99 weeks of age, GnRHa-Rec rams took longer to traverse a familiar spatial task, 4 weeks after training, than age-matched Controls, whereas their performance during an unfamiliar spatial task was the same as the Controls. This reduction in long-term spatial memory in GnRHa-Rec animals was also observed prior to the withdrawal of GnRHa-treatment (Hough et al., 2016) and the current study therefore reports that this reduction persisted into adulthood and was not reversed after the discontinuation of peripubertal GnRHa-treatment. A detailed discussion on these main observations follows below.