Effect of Vps13c deletion on calcium signaling in vitro in female mouse islets. Isolated islets from female mice (20–23 wk of age) maintained on RC (A) or HFD (B) were loaded with Fluo 2 and analyzed as for male islets (Fig. 10). (i), mean free calcium (F/F0); (ii), inset from (i), mean free calcium measured between 300 and 500 s, showing stimulation with 16.7 mM glucose; (iii), AUC analysis for high glucose stimulation; (iv), AUC analysis for KCl stimulation; (v), time to maximum peak value from stimulation with glucose; (vi), maximum peak value (F/F0) from stimulation with glucose; (vii), time to maximum peak value from stimulation with KCl; (viii), maximum peak value (F/F0) from stimulation with KCl; n = 3–5 mice per genotype. Number of islets used: female RC, n = 47–48 islets from 4 mice; female HFD, n = 46–59 islets from 4 or 5 mice (KO vs. Ctrl, respectively). *P < 0.5; **P < 0.01; ***P < 0.001; unpaired Student's t-test.