To characterize the observed TaFlo2-A1 haplotypes in large wheat populations, a molecular marker based on the 8-bp InDel observed in the promoter region was designed and named TaFlo2-Indel8 (Table 1). The forward and reverse primers of TaFlo2-Indel8 are located at −80 bp and 72 bp from the start codon, respectively. The PCR products of TaFlo2-Indel8 in the accessions with or without the 8-bp InDel have lengths of 153 bp and 145 bp, respectively. The bands of 153 bp and 145 bp were easily discriminated by polyacrylamide gel electrophoresis and represented the haplotypes TaFlo2-A1a and TaFlo2-A1b, respectively (Fig. 2c).