A poisoned food technique was used to evaluate antifungal activity against P. myriotylum . The oil extracts and two compounds (9-oxo-agerophorone and 9-oxo-10, 11-dehydro- agerophorone) were prepared using dimethyl-sulfoxide (DMSO, 0.5% v/v) as the initial solvent carrier followed by dilution with PDA (at about 50°C) to produce the desired concentrations of 40, 60, 80, 100 and 120 μg/ml. A 4 mm mycelial disk was cut from the periphery of 2-day-old cultures, placed in the center of each PDA plate, and then incubated in the light-dark cycle at 28 ± 1°C for 7 days. PDA plates treated with an equal quantity of DMSO were used as a negative control. Each treatment was repeated in triplicate. The mycelial growth (mm) in both treated and control Petri dishes were measured diametrically in two different directions using a vernier caliper. The percentage inhibition of fungal growth was calculated using the following equation when the mycelial mass had almost filled the Petri dish in the control: Inhibition(%)=[(C−T)/(C−4)]×100 Where C is the diameter of fungal colony in the control; T is the diameter of fungal colony in the treatment; and 4 is the diameter of the inoculum disc.