Quantitative real-time PCR (qPCR) reactions were performed using Fast SYBR Green Master Mix (Thermo Fisher) in Applied Biosystems 7900HT Fast Real-Time PCR system according to the manufacturer’s instructions. The cycling conditions were 95 °C for 10 min followed by 40 cycles of 95 °C for 10 s, 60 °C for 30 s. At the end of the last cycle, the temperature was increased from 65 °C to 95 °C (0.1 °C/s) to produce a melting curve. The specificity of amplification was assessed for each sample by melting curve analysis. Each PCR product showed a single peak. The size of the amplicon was checked by electrophoresis. Agarose gel electrophoresis revealed a single product of the expected size. The analysis of the expression of TLR3 relative to the β-actin was performed with software in relative quantification mode following the manufacturer’s instruction. The following criteria of sequences of all primers used in this study were applied in the course of designing the primers: product size from 100 to 500 bp, primer size from 17 to 23 bp, and a mean melting temperature of 60 °C.