The global prevalence of T. vaginalis and the health sequelae associated with it have necessitated the need to understand its genetic make-up. Trichomonas vaginalis is a complex pathogen, with a genome size of ~160 megabases, two-thirds of which is composed of repeats and transposable elements . Some strains of T. vaginalis can harbor up to four types of Trichomonas vaginalis viruses (TVVs) . TVVs are members of the family Totiviridae under the distinct genus Trichomonasvirus . Carriage of TVVs has been suggested to upregulate pro-inflammatory host responses  and T. vaginalis immunogenic protein P270  and is also associated with differential qualitative and quantitative expression of cysteine proteinases . Thus, since TVVs induce various phenotypic changes that may impact T. vaginalis virulence , determining carriage of TVVs seems to be essential in the characterization of T. vaginalis infection. However, no method has been adapted as a standard clinical diagnostic test for TVVs .