CaLC2213: To C terminally HA tag CAS5 in SN95, the HA-HIS1 cassette was PCR amplified from pLC57562 using primers oLC2161 in combination with oLC2162 and transformed into CaLC239 (SN95). Transformants prototrophic for histidine were PCR tested for correct integration of the cassette with oLC2163 in combination with oLC2029 and oLC2164 in combination with oLC1645.