This high-salt challenge induced development of hypertension within the experimental period. Figure 2B shows a representative increase of systolic (black) and diastolic (red) blood pressures in a Dahl SS rat infused with vehicle. The BP elevated in two phases. The first stage was characterized by a sharp increase occurring over 1–8 days after the change of diet with an increase in MAP by ~21 mmHg. Then BP rose slowly by ~8 mmHg over 8–14 days from the switch in diet. Finally, during the 3rd week after switching to a high-salt diet there was a second acute phase when MAP rapidly rose up to 171.8 ± 5.6 mmHg (Figure 2C, black dots).