The list of known GC driver genes was collated from the Cancer Gene Census79 and the driver genes identified by TCGA19 and Wang et al.20 We excluded TP53 from the analysis as TP53 frequently undergo deletions and loss of heterozyosity. We identified non-synonymous and truncating mutations on known GC driver genes, and compared their VAFs to the VAFs of CBS hotspot mutations from the same samples using a matched Wilcoxon rank-sum test. Only mutations in diploid regions in each sample were included in the analysis.