To obtain information about the ratio of the stabilized dimers and monomers, aliquots from the stock solutions (200 μM) were loaded directly into SDS-PAGE. As shown in Fig. 1b, substantial fraction of all TPPP/p25 solutions contains disulphide-stabilized dimers. The stock solutions of the FL and the different truncated forms diluted to 1 μM were applied for sandwich ELISA experiments according to the following experimental design as illustrated schematically, the result are presented in Fig. 1c,d: