CaLC3151: To C terminally HA tag CAS5 in a mutant heterozygous for CAS5, the CAS5-HA-HIS1 cassette was released from pLC790 with ApaI and transformed into CaLC2034. Transformants prototrophic for histidine were PCR tested with oLC2163 in combination with oLC2029 for upstream integration and oLC2164 in combination with oLC1645 for downstream integration. This strain shares the same genotype as CaLC3113 but was generated as a control for strains made using plasmid pLC790 as the backbone, such pLC791 and pLC800.