Comparative analytical sensitivity of the lyophilized PCR assay used to detect CaPV DNA. (a) A decimal dilution series of CaPV DNA (isolate Israel LSD‐07 POX‐V1‐07‐08) tested using wet reagents (●) and lyophilized reagents (○) with a laboratory‐based PCR machine (Mx3005P, Stratagene). Points represent mean CT from duplicate determinations where the maximum CT range of duplicates was 1.53. (b) Comparison of a decimal dilution series of a CaPV isolate DNA (Israel LSD‐07 POX‐V1‐07‐08) spiked into skin suspensions assayed using wet PCR reagents (○, Mx3005P) compared to lyophilized PCR reagents and nucleic acid extraction employed on the Enigma FL (●). Points represent mean CT from duplicate determinations for the wet PCR assay (* data point represents a single where the duplicate sample generated a no CT result), while only single values are shown for the samples tested on the Enigma FL.