The three lots of RM 8671 were found to conform to previously observed results for PS 8670 for all charge parameters whose values (ESM Table S6) agreed to within ±3uc, as shown in Fig. 3 for the main peak purity. The one-way two-tailed ANOVA, however, did reveal a minor difference from PS 8670. RM 8671 lots were found to contain slightly increased basic variants (≈1%) relative to PS 8670. This difference may be attributable to expected minor variations in the cellular expression/production of batches of material used for PS 8670 versus RM 8671. The three lots originating from the homogenized bulk (RM 8671), however, were shown to be consistent with one another in terms of main peak purity, basic variant relative abundance, and acidic peak relative abundance. Figure 3 demonstrates that good inter-lot reproducibility was achieved for each of the three 14HB-D lots, and that each material was observed to have main peak purity to within ±3uc of one another. Despite a small decrease in apparent charge purity versus PS 8670, it is the consistency of the commercial (RM 8671) lots that are important in ensuring a consistent product to stakeholders, which the current data indicate will be true considering the statistical equivalence of all three commercial lots. The current data suggest that the bulk homogenization method used to produce the RM 8671 lots was successful in homogenizing any minor process related variability (e.g. C-terminal lysine occupancy) and produced highly consistent, reproducible lots, thereby ensuring long term consistency in product quality attributes.