(A) and (B) Mean and SD (n = 3) of FLM pre-mRNA levels. Student’s t-test: ***p≤0.001; n.s. = not significant. (C) Schematic representation of FLM cDNAs detected more than once (n = 163). FLM-δ transcripts were not detected and are only shown for completeness. Grey areas correspond to FLM exons of the FLM-ß and FLM-δ gene models as specified in Figure 1A. The arrow indicates the position of the 3' RACE primer used in combination with an oligo(dT) reverse primer to detect polyadenylated transcripts. (D) Frequency distribution of the transcripts displayed in (B) with total number of sequences depicted in the graph.