The dose of PCPA (500 mg/kg) was selected based on evidence that PCPA remarkably decreases brain and serum 5-HT levels in mice [5, 6]. The dose of liraglutide (100 μg/kg) was selected based on evidence that liraglutide acutely induced hypophagia in mice [2, 7]. The dose of TCB-2 (2.5 mg/kg) was selected based on evidence that TCB-2 had no significant effect on food intake .