ANOVA and Chi-squared were performed online as referenced in Tables 1 and 2. T-tests were performed on line at http://www.graphpad.com/quickcalcs/ttest1.cfm.Table 1Influenza matrix and subtype gene seasonal nAMP averages in 3 institutions over 3 influenza seasonsInfluenza season2012–2013 H3N2 nAMPs ± SD (n)2013–2014 H1N1 nAMPs ± SD (n)2014–2015 H3N2 nAMPs ± SD (n)PMatrix Gene UF Health121.1 ± 109.5 (91)1 172.6 ± 70.2 (194)2 65.1 ± 37.5 (172)< 0.00014 BayCare74.8 ± 77.8 (19)165.4 ± 76.1 (232)50.2 ± 25 (152)< 0.00014 MemorialND3 160.1 ± 80.6 (475)57.3 ± 61.1 (788)< 0.00014 Subtype Gene UF Health169.9 ± 63.6 (91)240.9 ± 103.4 (194)164.3 ± 40.1 (172)< 0.00014 BayCare136.1 ± 50.2 (19)249.2 ± 81 (239)162.4 ± 45.9 (165)< 0.00014 MemorialND239.4 ± 88.6 (475)144.6 ± 53.0 (711)< 0.00014 1() = number of specimens tested 2Includes 22 patients from 1/21/2013–8/30/2013 who were positive for H1N1 3ND = Not Done 4ANOVA http://vassarstats.net/anova1u.html Table 2Ct adjusted nAMPs and GenMark RVP Matrix Gene Sequence MismatchesInfluenza StrainMatrix Gene nAMPsa Matrix Gene MismatchesForwardReverseCaptureSignal A/NY53.5 ± 32b 1c 03d 0 A/Texas56.6 ± 11.4b 1030 A/Switzerland46 ± 16.9b 1030 H3N2 Synthetic Capture Probe ssDNAe 73 ± 16N/Af N/A3N/A H1N1143.6 ± 57.21020 H1N1 Synthetic Capture Probe ssDNA151 ± 17N/AN/A2N/A aMean ± SD of 4 replicates. All 4 strains were diluted to the same copy number based on Ct using a TaqMan assay (see Methods) bA/NY (p = 0.033), A/Texas (p = 0.025) and A/Switzerland (p = 0.017) vs H1N1 t test. https://www.usablestats.com/calcs/2samplet cThe mismatches were the same in all H3N2 strains, but different from the H1N1 dAll 3 mismatches were the same for the H3N2 strains, but differed from the 2 mismatches in the H1N1 strain essDNA = single stranded DNA fN/A = not applicable