To identify the representative metabolites in B16 cells in response to radiation, we firstly compared 1H NMR spectra from X-ray radiated B16 cells and untreated control cells. The representative spectrum of cellular soluble metabolite from X-ray radiated B16 cells and untreated control cells was indicated in Fig 1A and 1B, where the spectrum peak with δ0.5–8.6 ppm chemical shift was kept and water peak was removed. With ChenomxNMR Suite software analysis and statistical total correlation spectroscopy (STOCSY), major metabolites in the spectrum were confirmed. Chemical shifts and corresponding groups were summarized in Table 1.