All statistical tests were run using IBM SPSS Software (version 24, Armonk, NY, USA). The strength and direction of associations were evaluated using the nonparametric Spearman rank-order correlation coefficient (Spearman’s correlation, for short) or the Pearson product-moment correlation coefficient (Pearson’s correlation, for short). Linear regression analysis was used to quantify how well two variables relate to each other. When two or more independent variables were hypothesized to affect the outcome, multiple regression analysis was used. When sample groups were not normally distributed, differences between groups were compared by related samples Wilcoxon Singed Rank Testing. All histogram charts represent single values. Significance was set at <0.05. For box-and-whisker plots, the center lines show the medians; box limits indicate the 25th and 75th percentiles, whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles. Box and whisker graphs were plotted using BoxPlotR (http://shiny.chemgrid.org/boxplotr/).