YTS cells were seeded in 24-well plates (4×104 cells/well). The viral supernatant with Lenti-Con (lentivirus-carrying vectors) and Lenti-GSN (recombinant lentivirus-carrying GSN cDNA) were added into the cells at a density of 70%-80%, respectively. After 72 h, the transfection ratio was determined under a fluorescence microscope and was measured by flow cytometry. The cells with a transfection ratio of >70% served as the target cells and were identified by qRT-PCR analysis.