To evaluate the effects of PA-MSHA on EGFR signaling in pancreatic cancer cells, we examined the expression of key downstream proteins in the EGFR pathway (t-EGFR, t-AKT, t-ERK, p-EGFR, p-AKT, and p-ERK) by western blotting. Interestingly, the levels of p-EGFR, p-AKT, and p-ERK decreased with increasing concentrations of PA-MSHA, while total EGFR, AKT, and ERK protein levels did not change (Figure 6). Thus, PA-MSHA inhibited EGFR signaling in pancreatic cancer cells by blocking phosphorylation of several downstream effectors of EGFR.