As expected, immunolocalization showed that CsTegu20.6 was dominantly distributed at the tegument of the C. sinensis adult worm, using anti-CsTegu20.6 sera as the primary antibody and fluorescein isothiocyanate (FITC)-conjugated anti-rat IgG as the secondary antibody (Figure 10A,B). In contrast, no staining was observed in sections incubated with the pre-immunized serum (Figure 10C,D).