Feedstock types and pyrolysis temperature are two main factors influencing biochar property18. As a wide variety of biomasses can be used for biochar production, previous studies on Zn2+ immobilization behavior of limited type of biochars are far from enough. To explore the immobilization mechanisms of Zn2+ on different biochars and provide more basic data for biochar application, studies which are focused on biochars produced by other waste biomasses need to be done. Although many studies have mentioned the effect of pyrolysis temperature on biochar property2031323334, few studies systematically investigated the effect of pyrolysis temperature on immobilization behavior of Zn2+ on biochar and elucidated the internal connection between pyrolysis temperature, biochar property, and immobilization behavior of Zn2+ on biochar. In this study, biochars produced by pine needle (PN) and wheat straw (WS) were chosen for investigation, to our knowledge, there is no published work concentrated on Zn2+ immobilization behavior of biochars derived from these biomasses. Two temperatures were chosen for biochar preparation, and the Zn2+ immobilization behaviors of biochars produced by different temperature were compared through several sorption experiments (i.e. sorption kinetics, sorption isotherms, and the effect of pH). To reveal the importance of the ash on biochars on Zn immobilization, the ash was removed from biochars, and the Zn2+ immobilization behaviors of de-ashed biochars were compared with those of the raw biochars. To explore the immobilization mechanisms, the methods of Zn K-edge EXAFS spectroscopy and X-ray Powder Diffraction (XRD) were used for biochar characterization. Through this study, the Zn species immobilized by PN and WS biochars will be identified, the immobilization mechanisms and the relation between pyrolysis temperature and immobilization behaviors of Zn2+ on studied biochars will be clear.