We did not find a pronounced difference of genetic diversity between native and expanding spider populations. This held true for allelic richness (AR) and expected heterozygosity (EH). The highest diversity was found in Russia (AR = 3.69, EH = 0.63), followed by Iberia (AR = 3.46, EH = 0.60) and Italy, then northeastern Germany (AR = 3.31 3.62, EH = 0.58), and finally, the lowest in the Baltic States (AR = 2.85, EH = 0.51). The only significant difference in diversity was found for the Baltic populations, which showed a reduction of allelic richness and expected heterozygosity compared to all other populations (anova, Games–Howell post hoc test, P < 0.01). Russian, Iberian, and populations from northeastern Germany did not show significant differences in diversity.