We further evaluated the immunomodulatory effects of AHG in a CY-induced immunosuppression murine model. CY, an alkylating agent, is an important chemotherapeutic drug in malignant tumor treatment. However, CY intake can injure DNA of normal cells and cause myelosuppression, immunosuppression and oxidative stress on various tissues, which sometimes are life-threatening . In the present study, we used CY as an immunosuppressive agent to establish a model of weakened immunity. As expected, CY resulted in immunodeficiency, as evidence by significantly reducing splenocyte proliferation, cytokine secretion, intracellular free Ca2+ concentration, and inhibiting SRBC-induced DTH reaction as well as damaging antioxidant system. These remarkable differences in various immune parameters between the CY-treated group and the NC group indicated that the immunosuppression model was applicable for in vivo experiments.