Data is all presented as mean ± SEM in the text and figures (Igor Pro, WaveMetrics, Inc., Lake Oswego, OR). Student t-tests, Analysis of Variance (ANOVA), and Kolmogorov-Smirnov test (K-S) tests were used to examine the difference between means and distributions using Statview (Abacus Concepts, Berkeley, CA, USA). Fisher’s protected least significant difference (Fisher’s PLSD) tests were used for pairwise comparison of groups following an ANOVA test. Chi-squared tests were performed using equations coded in Excel (Microsoft Crop., Redmond, WA, USA). The level of statistical significance was set at 5%. In all figures, * indicates P<0.05; ** indicates P<0.01; and *** indicates P<0.001.