After 72 h of transfection, untreated, Non-T siRNA/miRNA- or miR-101-treated cells were counted and seeded 800 cells per one well of 6-well plates (triplicate) and incubated at 37 °C with 5% CO2 for 7–10 days. Colonies were fixed with 10% (v/v) ethanol for 30 min and stained with crystal violet (Sigma-Aldrich, St Louis, MO, USA) for 20 min. Then, the photographs of the colonies were taken using Amersham Imager 600RGB (GE Healthcare Life Sciences, Pittsburgh, PA, USA). Colony quantification was carried out using ImageQuant TL Colony v.8.1 software (GE Healthcare Life Sciences).