10.7554/eLife.24635.016Figure 5—figure supplement 1.Assessment of spontaneous cassette inversion.(A) Schematic of the inverted shhact allele and primers sites. (B) The inverted allele was not detected in untreated tcf21:CreER; shhact/ct embryos using 40 cycles of PCR. (C) The inverted allele was not detected in untreated adult tcf21:CreER; shhact/ct fish using 30 cycles of PCR, but it was detected when the cycle number was increased to 35. Each PCR sample used genomic DNA isolated from five pooled hearts as a template.DOI: http://dx.doi.org/10.7554/eLife.24635.016