All the genes detected and specific primers used are listed in Table 1. Quantitative real-time PCR was performed using a CFX Connect™ Real-time PCR Detection System (Bio-Rad, Hercules, CA, USA) with a SYBR Premix Ex Taq™ II PCR kit (TaKaRa, China). The PCR conditions were as follows: 95°C for 1 min, 40 cycles of denaturation at 95°C for 15 s, annealing at 60°C for 30 s, and extension at 72°C for 30 s. Melting curve analyses were performed to monitor the purity of the PCR product. All reactions were run in triplicates. Relative gene expression levels were evaluated through the 2−ΔΔCt method, where ΔΔCt = (Ct, target Ct, β−actin)treated group (Ct, target Ct, β−actin)control group.