It must have escaped the reviewer’s notice that we already built a 34-protomer model into the smaller pore of the subtomogram average, and that this was shown in Figure 6E of the original manuscript. No significant modification of the pore subunit, in particular not of the 20° incline of the β-hairpins relative to the membrane normal, was necessary to fit 34 copies of the pore-forming subunit into the 27 Å-resolution cryoET map. This is now pointed out more clearly in subsection “Three stages of PLY pore formation” of the revised manuscript