Chromatin immunoprecipitation assays were performed as described69. Quantitative PCR was performed using oligonucleotide primers that amplify a fragment of the mouse Foxo1 gene spanning nucleotides −880 to −751 relative to the transcription initiation site. Their sequence is as follows: Forward, 5′-TGCGACTTCAACACCTCATC-3′; reverse, 5′-CGGTGTGGTGGCTAAAGAGT-3′. SYBR Green detection was used with Power SYBR Green PCR Master Mix (Applied Biosystems). Data were calculated as fold enrichment relative to input.