The crude extracts from W. anomalus ATCC 96603, UM3, and 1F1 were analyzed by non-continuous denaturing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE) in 7% polyacrylamide gel, at 100 Volts for 2 h in a minigel system (Bio-Rad Laboratories, Hercules, CA, USA). Concentrated YPD medium was also run as a control.