Glaucoma can result in irreversible vision loss. At this time, the only proven treatment is lowering intraocular pressure (IOP) . IOP is elevated in glaucoma due to increased resistance to fluid outflow from the eye . Methods for IOP lowering include topical medicines, lasers, and invasive surgeries. Today, a new category of glaucoma surgery, Minimally Invasive Glaucoma Surgeries (MIGS) , is of great interest. MIGS offer a small wound, safe, and fast surgical approach to enhance aqueous humor outflow (AHO) drainage. With some of the early MIGS procedures, there has been inconsistent success [7–10]. Therefore, the importance of furthering our understanding of how aqueous humor exits from the eye is of increasing interest. Using new and emerging technologies, AHO structure and function is being re-examined with a goal of improving current and enhancing future therapeutics.