Fluorescein isothiocyanate (FITC)-conjugated gelatin matrix-coated coverslips were prepared as described previously . After the coverslips were quenched for 1 h with complete media at 37 °C, the cells were plated and cultured in medium with DPB-LPS (1 μg/ml) and/or EETC (10 mg/ml). Cells were fixed with 4% paraformaldehyde followed by permeabilization with 0.5% Triton X-100/PBS. Dark areas lacking fluorescence on the coverslips were observed with a LSM 510 META confocal laser scanning microscope (Carl Zeiss, Oberkochen, Germany).