Our findings of the lower limit of lethal temperatures of 7 °C for larvae coincides with published calculations of the minimum thermal temperature for larval development of 7 °C . Scott  showed that no male Asian bush mosquitoes and only 50% females of a New Jersey, USA, based laboratory colony pupated at 10 °C. Here, we show that individuals of both sexes of a German population can reach the adult stage at this temperature. We also could show that some larvae could survive for up to three days at 0 °C as first-instars. Thus, it is possible that both sexes of the Asian bush mosquito can temporarily survive low temperatures in the larval stage. In Japan, the Asian bush mosquito overwinters in the larval stage , but outside its native range, no larval overwintering was observed yet. Our results show that larval overwintering in Germany is not possible because at least one frost event occurs (Fig. 4). However, under suitable microclimatic conditions, e.g. in anthropogenic microhabitats in cities, larval winter survival may be possible, especially in western Germany.