The results for collagen content follows the effect of normalization15 with depletion of GAG allowing for collagen to redistribute within the structure of ECM, therefore appearing to measure at an almost similar concentration with no statistical difference to native. This is due to a decrease in the wet weight of the cartilage specimens. The collagen level measures in association to a reduced wet weight post decellularization therefore appearing at a higher concentration. The wet weight decreased consistently for all protocols at their end points. The percentage decrease relative to native tissue is demonstrated in Fig. 7.Figure 7Mean percentage reduction in wet weight compared to native (N = 3). Error bars for SD.