Formalin-fixed kidney sections were cut at 4 μm slices, dried and deparaffinized for subsequent streptavidin-biotin immunohistochemistry. After deparaffinization, the slides were treated with a citrate buffer (pH 6) for a total of 35 min. The slides were blocked with a peroxidase block (DAKO, Carpinteria, CA, USA), Avidin Block (Vector Labs, Burlingame, CA, USA), Biotin Block (Vector Labs), and serum-free Protein Block (DAKO). For kidney damage analysis, the tissue was stained with Masson's trichrome. For Glut2 staining, liver sections were embedded in one paraffin block for tissue microarrays and slices were incubated 1:200 overnight with anti-Glut2 antibodies (#sc-9117, Santa Crus Biotech, Dallas, TX). Secondary detection was performed with Goat anti-Rabbit biotinylated IgG (Biocare, Concord, CA, USA) followed by Streptavidin HRP (Biocare) and visualized with Diaminobenzadine (DAB, DAKO). ImageJ software was used to assess intensity of Glut2 staining in hepatocytes. Average signal levels in 10 areas in each liver were measured, and background (counterstained samples treated with secondary antibodies) was subtracted.