A similar analysis was applied to CD8+ T cells. We detected a significant increase in total IFNγ+CD8+ T cells frequency in DENV/ZIKV‐patients compared to exposed healthy donors. A trend toward increased frequency of total IFNγ+CD8+ T cells was observed in ZIKV and in DENV patients (except for patient 2, open squares) (Fig. 2g). Exposed donors, DENV, ZIKV, and DENV/ZIKV‐patients had similar frequency of total TNF+CD8+ T cells (Fig. 2h). A decreased frequency trend of total IL2+CD8+ T cells, similarly to total IL2+CD4+ T cells, was detected in all acute patients compared to exposed donors (Fig. 2i). Regarding the multifunctional analysis, we only detected a higher frequency of single IFNγ+CD8+ T cells in DENV‐ and DENV/ZIKV‐patients compared to exposed donors (Fig. 2e). Therefore, we suggest that DENV/ZIKV coinfection may influence differently CD4+ and CD8+ T cells responses, an effect mainly observed in the frequencies of CD4+IFNγ+TNF+, CD4+TNF+IL2+, and CD8+ total IFNγ+ populations.