For intracellular cytokine staining (ICS), PBMCs (2 × 105 cells/well) were incubated without stimuli or with PMA (10 ng/mL)/Ionomycin (1 μg/mL) for 2 h at 37°C. Then, Brefeldin A (10 μg/mL) was added to the cultures and incubated for 4 h. Cells were then washed and stained for extracellular markers for 30 min using BV510 anti‐CD3, PECy7 anti‐CD4, and PETexasRed anti‐CD8. After that, cells were washed and fixed with 2% paraformaldehyde. For ICS, cells were blocked with bovine serum albumin (1% BSA, Sigma–Aldrich), permeabilized with saponin (0.05%) and stained with eFluor® 660 anti‐IFNγ, Alexafluor® 700 anti‐TNF, and eFluor® 450 anti‐IL2 (Table S1). Samples were analyzed on BD® FACS ARIA IIu flow cytometer and analyzed using FlowJo 10 software (Tree Star®).