A total of thirty healthy female BALB/c nude mice (4 weeks old) were provided by Shanghai SLAC Laboratory Animal Center of Chinese Academy of Sciences (Shanghai, China), and maintained in specific pathogen-free (SPF) conditions. MDA-MB-231 cells were injected subcutaneously into the right armpit of the nude mice at 4 × 106 cells in 150 μl PBS. After 15 days incubation, three mice with the largest tumors and two mice with the smallest tumors were excluded, and the other twenty-five xenograft mice with tumor size reaching a diameter of about 5 mm were randomly divided into five groups. The Cos, Dehy, CD and VOSL-treated groups were injected intraperitoneally with the corresponding agents at a dose of 20 mg/kg/day, respectively. The negative control (NT) was treated with an equal volume of vehicle. Tumor size was monitored at 0, 3, 10, 17, 24 and 31 days post-treatment, and tumor volume was calculated using the formula: “maximum diameter × minimum diameter2/2. Tumor-bearing mice were sacrificed after 30 times of administrations. Tumors were harvested and weighed. The tissues were cut into consecutive sections for examining the expression of p-AKT, p53, p-14-3-3 and c-Myc by immunohistochemistry. All procedures involving animals were reviewed and approved by the Experimental Animal Ethics Committee of Second Military Medical University, and we confirm that all experiments were performed in accordance with relevant guidelines and regulations.