The NGEN assembly resulted in a total of 28,592, 27,544 and 44,065 contigs for pools of BBD-resistant, BBD-susceptible and the combination of both libraries, respectively. NGEN assemblies incorporated 76%, 73% and 73% of the sequence reads from the BBD-resistant, BBD-susceptible and the combined samples. NGEN contig lengths averaged approximately 360 bases, with a median varying from 475 to 542 bases. The longest transcripts obtained in the resistant, susceptible and combined assemblies were 11,704, 8168 and 10,800 bases, respectively. Table 2 summarizes the results of the three F. grandifolia transcriptome assemblies using the NGEN program.Table 2 F. grandifolia transcriptome NGEN assembly statisticsBBD-Resistance LibrariesBBD-Susceptible Libraries“Combined” Reference transcriptomeAssembled Reads533,261521,5051,026,995Unassembled Reads167,324184,226379,321Total Number of Reads700,585705,7311,406,316Assembled Reads (%)76.1273.9073.03Assembled Contigs28,59227,54444,065Contigs >2 K6222711115Av. Length of Contigs362354357