In summary, we identified CsGRN as belonging to the granulin family through bioinformatics and phylogenetic analyses. We also expressed and purified soluble rCsGRN in E.coli and discovered it was an important component of CsESPs. In adult worm, CsGRN is mainly located in the tegument and testes, which might be involved in parasite growth and development or even in the pathopoiesis of the parasite. CsGRN could be detected at a high expression level in clonorchiasis-induced Balb/c mouse liver tissues and was even observed in hepatocytes and cholangiocytes. In addition, the secretory eukaryotic expression vector pEGFP-C1-CsGRN was generated and employed to intervene in the expression and secretion of CsGRN in PLC and RBE cells. An enhancement of cell migration and invasion was observed, and the current results suggested that CsGRN is likely to promote cell migration and invasion by inducing liver EMT via the ERK and PI3K/AKT signalling pathways. The present study supports the involvement of CsGRN in the pathogenesis of CCA and HCC.