The apical patency was checked again by passing a #10 file through the apical foramen. Paper points were used to dry the root canals and standardized gutta-percha points (Dentsply-Maillefer, Switzerland) with proper tug-back at the working length were selected as master gutta-percha points. In groups 1 and 3 MTA Fillapex sealer (Angelus, Londrina, Parana) was used. Adseal sealer (Meta Biomed, Cheongju, South Korea) was used in groups 2 and 4. The obturation was completed with cold lateral condensation technique in all the experimental groups. The teeth were filled with gutta-percha without sealer in the positive control group and kept empty in the negative control group.