Time lapse experiments, similar to those carried out with the full-length Aβ42 peptide as described above, were also performed for the Aβ(14-23) peptide. The same surface area was imaged continuously after injecting 100 nM of Aβ(14-23) peptide in 10 mM sodium phosphate buffer in a fluid cell. Supplementary Fig. 4A shows typical images of aggregates at different time intervals: frame (i) 10 minutes after protein addition; (ii) 4 h; (iii) 5 h, and (iv) 6 h respectively. Frame (i) illustrates the presence of a very small number of globular aggregates after 10 min of peptide injection. As time progresses, the number and size of aggregates increases as shown in frames (ii) to (iv). Dynamics of association/dissociation of amyloid aggregates with the surface were also observed in time lapse experiments for this small fragment Aβ(14-23). The black circles in frames (ii), (iii) and (iv) indicate a subset of aggregates and serve as markers to monitor the changes occurring in other aggregates. A few examples of association (green dotted circle) and dissociation (yellow dotted circles) of Aβ(14-23) aggregates are shown in frames (iii) and (iv). The statistics on number and size (volume) of globular aggregates over time are shown in Supplementary Fig. 4B indicating a monotonic increase in both variables with time.