Finally, another approach to increase the content of bioactive compounds has been recently considered and is represented by plant cell cultures in vitro. This method offers several advantages when compared with whole plants. For example, using sterilized containers, pathogens are avoided, as well as an undesirable distribution of pollen and cross-fertilization. In addition, cultured plant cells need simple nutrients to grow. Moreover, the purification of the bioactive compound is facilitated since complex plant fibers are not present with the consequent reduction of production costs . These factors can allow for further optimizing the culture conditions, and, thus, increase the bioproduction of glucosinolates .