Adult ticks were collected from February to May 2014 from 4 different areas of San Diego County: Escondido Creek, Los Peñasquitos Canyon, Lopez Canyon and Mission Trails Regional Park by dragging a 1 m2 piece of canvas over grass and chaparral and then capturing the ticks with forceps and placing them in individual sterile microfuge tubes. The ticks were transported live back to the Vector Disease and Diagnostic Laboratory at the San Diego County Operations Center where, by visual examination, their species and sex were determined and cataloged before freezing them at −80 °C.