The univariable analyses for risk factors showed significantly higher carriage frequency in cats ≥ 8 years old compared to younger cats (P < 0.05), and a higher prevalence among domestic cats compared to purebred cats (P < 0.05). One of the cats (Additional file 1: Table S1, no. 42) was diagnosed as feline immunodeficiency virus (FIV) positive and was also infected with CMhm. Among males, 23.5% (8/34) carried haemoplasma species, whereas only 9.4% (3/32) of the females were positive. Although, prevalence in males seemed higher than in females, the difference was not statistically significant (P > 0.05).