Since the advent of material science and biomaterials, other options have been experimented with. MedPor® ear scaffolds composed of porous polyethylene have been applied clinically, however, they have reported a high incidence of extrusion post implantation13. The use of different synthetic scaffolds for auricular repair has demonstrated some difficulties in mimicking the native ECM as well as cytotoxicity and degeneration obstacles13. An alternative option is the use of decellularized scaffolds, as they mimic the ECM. A native ECM offers a more biocompatible approach for auricular repair and decellularization of ear cartilage has so far successfully offered a scaffold, which is capable of inducing cartilage formation14. However, the understanding into decellularization protocols for human auricular cartilage is limited. In the study by Utomo et al.15, the number of cycles required to achieve optimum decellularization of ear cartilage is not specified, this makes it difficult to replicate by other research groups. Also their study design does not compare different decellularization protocols which limits the evaluation of their proposed method. Gong et al.16 have briefly referred to a protocol however they do not provide intricate detail of the experimental steps. In addition, their decellularized cartilage was of a porcine source.