The observation of two divergent mitochondrial haplotypes in the Russian and Baltic expanding populations resemble recent findings in Argiope bruennichi, another expanding spider species (Krehenwinkel and Tautz 2013; Krehenwinkel et al. 2015). Here, an admixture of two formerly separated lineages relates with rapid divergence of expanding and native populations. This finding is in line with many recent studies that associate expansion success and genetic admixture (Kolbe et al. 2004; Nolte and Tautz 2010; Rius and Darling 2014). Interestingly, our microsatellite analysis suggests the contact of a western and eastern European native genetic cluster in Central Europe. Expanding populations might thus have been founded from admixed populations. However, a deeper geographic sampling and more genetic data will be necessary to quantify an association of admixture and expansion.