MTSS1 expression is significantly decreased upon exposure to cmvIL-10. a MDA-MB-231 cells were cultured with 100 ng/ml cmvIL-10 for the indicated times and lysates examined by western blotting with anti-MTSS1 or anti-β-actin as a protein loading control. b MDA cells were seeded onto glass coverslips in the presence of absence of 100 ng/ml cmvIL-10 for 96 h, then fixed, permeabilized, and stained as indicated. Scale bar 10 μm. These results are representative of three independent experiments