Trichomonas vaginalis actin sequences were edited using Chromas Lite 2.01 (http://technelysium.com.au/wp/). Furthermore, we identified the T. vaginalis actin genotypes amongst our isolates by means of in silico RFLP-analysis Webcutter version 2.0; http://rna.lundberg.gu.se/cutter2/with the three restriction enzymes (HindII, MseI and RsaI) used by Crucitti et al. . The in silico analysis was done on our clinical isolates and on retrieved sequences representing the genotypes that have been proposed. To identify if there were additional T. vaginalis actin genotypes not captured by the previous proposed scheme, in silico RFLP was performed on actin sequences retrieved from the GenBank whose actin genotype is yet to be documented. The sequences were of at least 1100 bp in length (Additional file 1: Table S1).