For the H3K27me3-enriched gene analysis between normal tissues and tumors, ChIP-on-chip data were processed using RINGO software 1.26.1, then the microarray data was analyzed on R software using several Bioconductor packages (www.bioconductor.org/). Enriched regions were defined from enriched probes using the criteria of at least 3 enriched probes within the region. For each probe on the array, a score was calculated as follows: score = ∑ (enrichment values Probes-Enrichment Threshold). Only genes with a threshold of >1.5 were considered as differently enriched. Genes with a score of least than 1.5 were removed from analysis, as were genes with missing data in more than 30% of the samples.