Compared to the MATLAB tool BitterPredict (Dagan-Wiener et al., 2017), e-Bitter adopts the ECFP fingerprint as the molecular descriptors, which is natively implemented in our program. Thus, e-Bitter does not depend on any other commercial softwares such as Schrödinger package required in BitterPredict. In addition, this free e-Bitter program employs the versatile machine-learning algorithms and works compatibly with the free python environment, while BitterPredict is developed and runs in the commercial MATLAB environment, which will restrict its extensive tests or applications for most of the users.