Using the sequences of the markers (including SNPs, PCR-based markers and DArTs), we conducted comparative genomics analysis against the contigs assembled in the chromosome survey sequencing (CSS) project. All of the contig sequences were downloaded from https://wheat-urgi.versailles.inra.fr/Seq-Repository. More recently, the genome assembly of T. aestivum cv. Chinese Spring (CS) has been released (https://urgi.versailles.inra.fr/download/iwgsc/IWGSC-WGA_Sequences/). Based on SNP flanking sequences, we assigned SNPs to this wheat genome assembly.