The auditory function was evaluated every month in both dietary groups. No significant changes in ABR threshold, latencies, or amplitudes were evident between NF and FD mice along the whole study (Table 2). Both NF-10M and FD-10M groups showed a decreased amplitude of wave I, which correlated with a slight increase in hearing thresholds. Histological analysis revealed damage in the organ of Corti (OC) at the low basal turn of the cochlea, together with a slight loss in type IV fibrocytes at the spiral ligament (Spl) and accumulation of melanin granules in the stria vascularis (StV; Figure 2A). Altogether, these alterations are indicative of the initial phases of hearing loss in both dietary groups, but seem more severe in cochleae of FD mice in which the absence of outer hair cells is noticed (Figure 2Ag).