LC-UV-MS/MS peptide mapping was performed on all three lots of RM 8671 in parallel with PS 8670 according to the optimized protocol described previously . Alignment of the TIC and UV traces of the RM 8671 digest with the PS 8670 reference peptide map showed a high degree of similarity upon visual inspection (Fig. 7). No trace had a unique or missing peak as compared to the reference map.Fig. 7Alignment of PS 8670 chromatogram with RM 8671 lots 14-HB-D-001, 14-HB-D-002, and 14HB-D-003 chromatograms. Tryptic digests of PS 8670 and RM 8671 lots 14-HB-D-001, 14-HB-D-002, and14HB-D-003 (traces ordered top to bottom) were analyzed by LC-UV-MS and the similarity of the resulting (A) TIC and (B) UV chromatograms compared against the reference peptide map generated from the PS 8670 digest (top, black trace in panels a and b). The initial five minutes of the UV traces are not shown due to the large difference in scale between the relative levels of absorbance of peaks detected during the 0 min to 5 min period and the 5 min to 90 min period