In this study we have used qPCR and immunohistochemistry to conduct the first comprehensive study of K2P channel expression in the mouse retina. We show that K2P channels are widely expressed in the retina, with multiple K2P channels detected within Müller cells and retinal ganglion cells. These data offer new insight into the mechanisms that regulate electrical activity within the retina and indicate that K2P channels likely perform important physiological functions within visual signalling pathways.