Barki and colleagues removed AG from juvenile intersex crayfish and observed that in the adult stage they did not fight with other males and did not initiate mating behaviors with females . We can see that AG has played a decisive role in male mating and aggressive behavior in E. sinensis. We found that the expression of octopamine receptor was significantly upregulated while 5-hydroxytryptamine receptor 1 was significantly downregulated in neuroactive ligand-receptor interaction pathway in SP AG (Figure S4). The function implementation of 5-HT and OA was regulated indirectly by AG. Neuroactive ligand-receptor interaction pathway analysis revealed the key regulation mechanism of melatonin, 5-HT, and OA, which have laid the foundation for further research.