It has been reported that central injection of GLP-1 promoted BAT thermogenesis, leading to increased energy expenditure (Lockie et al., 2012; Beiroa et al., 2014). We thus examined if peripheral injection of supaglutide enhanced BAT activity in the obese mice. Ucp1 is generally considered as a marker of BAT activity because it is highly expressed in BAT and dissipates the proton gradient across the mitochondrial inner membrane to produce heat (Stanford et al., 2013). Interestingly, we found that although supaglutide had no effect on Ucp1 expression in BAT (Figure 7A) and epidydimal WAT (Figure 7C), it significantly upregulated the expression of Ucp1 in the inguinal WAT (Figure 7B). This suggests that supaglutide does not enhance BAT thermogenesis but promote browning of inguinal white adipose tissue.