Before qPCR analysis, RNA should be processed by a reverse transcription step to obtain  complementary DNA (cDNA). cDNA synthesis was accomplished by ToolScript MMLV RT kit. Briefly, 1 µg total RNA of each sample was mixed with 2 µL oligo dT, 2 µL dNTP, and distillation-distillation H2O to bring the total volume to 14.5 µL. To open the RNA secondary structure, the RNA mixture was heated at 70 °C for 5 min and then cooled on ice for 2 min. To the cooled mixture were added 4 µL reverse buffer, 0.5 µL RNasin, and 1 µL MMLV. Afterward, the mixture was incubated at 42 °C for 50 min to synthesize cDNA and then heated at 95 °C for 5 min to inactivate the MMLV. Further, the cDNA products were processed on qPCR using TOOLS 2× SYBR qPCR Mix. First, a reaction solution was prepared containing 10 µL 2× SYBR qPCR mix, 0.1 µL forward primer, 0.1 µL reverse primer, 1 µL cDNA template, 2 µL of 50× ROX reference dye, and distillation–distillation H2O to bring the total volume to 20 µL per well. Next, the qPCR machine (ABITM StepOneTM Plus, Thermo Fisher) was set up. The initial denaturation temperature was 95 °C for 15 min by one cycle, followed by a denaturation temperature of 95 °C for 10 s, annealing at 60 °C for 20 s, and extension at 72 °C for 30 s, with the total PCR stage being 40 cycles. The designed forward and reverse primers from 5′ to 3′ used in this experiment are shown in (Table 2) .