Whilst the depletion of DNA to a significant amount was desirable for the decellularization pathway in protocol A, a 35 day cycle however lead to significant morphological changes with denaturation of the 3D ECM structure on SEM when compared with native. Protocol C with EDTA did not produce the same level of reduction in DNA as did protocol B with trypsin after 14 cycles of chemical decellularization. Protocol B however, was able to reduce it in a short time whilst preserving the three dimensional structure of the cartilage.