Previously, we reported the detection of a novel kobu-like virus in Japanese black cattle by deep sequencing method . During the metagenomics surveillance, nucleotide sequences with high similarity to BEVs were identified in feces collected from a calf with diarrhea. This feces was collected from a 1-month-old calf with diarrhea in Kagoshima prefecture (Kagoshima sample) in 2014. No other clinical sign was observed except diarrhea. Feces was collected directly from the rectum on the onset day. One gram feces was diluted with 9 mL PBS (−) to prepare a 10% fecal suspension and centrifuged at 10,000 × g for 10 min. The supernatant was collected and stored at −80 °C before RNA extraction and virus isolation.