Selected, healthy fresh ginger rhizomes were collected from Shandong Province, China. They were washed in running water, dipped in 70% ethanol for 5 min, and then washed 4 times with sterile water. The surface-sterilized ginger rhizomes were cut into thin slices (2–3 mm). Three concentrations of E. adenophorum oil extracts (100, 160, 200 μg/ml) were prepared with 0.5% (v/v) DMSO and 0.01% Tween-80. Each ginger slice was dipped separately in the oil extracts for 5 seconds and placed into a sterile Petri dish (9 cm in diameter), then inoculated with P. myriotylum by placing a 4 mm disc of mycelial material cut from the periphery of 2-day-old culture. All treatments consisted of three replicates with two ginger slices per replicate, and the entire experiment was repeated twice. The controls were prepared in similar manner using a mixture of 0.5% (v/v) DMSO and 0.01% Tween-80 instead of E. adenophorum oil extracts. All the Petri dishes were incubated at 28 ± 1°C for 4 days and the percentage of infected ginger was observed and recorded.