Urine pyrophosphate concentration (U-PPi, A) following an overnight fast and renal gene expression as indicated on the y-axis for ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1, B), progressive ankylosis (Ank, C), ectonucleoside triphosphate diphosphohydrolase 5 (Entpd5, D), tissue nonspecific alkaline phosphatase (Tnsalp, E) in mice fed regular chow for 10 weeks. The data represent mean±SEM of 4–19 mice, p-values shown above the lines of comparisons were calculated by one-way ANOVA using Tukey’s adjustment for multiple comparisons (A) and Student’s t-test (B-E).