2) The use of pools of independent segregating T2 lines for transcriptional analysis seems unusual. While the controls presented in Figure 2—figure supplement 2 suggest that this can recapitulate the “natural” situation (why not subjected to statistical analysis?), it seems nonetheless risky to deliberately tolerate the presence of non-transgenics in the mix. Could the authors state their reasoning for this unusual procedure and provide the missing statistics here? Also, the phrase “replicate pools compromising…” should probably read “comprising”.