A. fumigatus akuAKU80 and its pyrG- derivative (akuAKU80 pyrG-) with a highly efficient homologous recombination background were used for isolation of AfuRbp gene homologue and gene disruption or gene silencing experiments. E. coli Top10 (Invitrogen, USA) cells and pGEM–T Easy cloning system (Promega, USA) were used in all DNA recombinant procedures. Plasmid PGEM-GlaA comprising Aspergillus niger glucoamylase A (glaA) promoter and glaA termination signal was used for preparation of RNAi plasmid. All molecular methods including PCR and RT-PCR were performed based on established protocols. In RT-PCR settings, 1 µg of total RNA was used in cDNA synthesis reactions and the A. fumigatus actin gene (AFUA_6G04740) was used as a loading control. Fungal strains were grown and kept on SAB agar or SAB agar medium supplemented with uridine and uracil. Modified Vogel’s medium was used in isolation of fungal transformants.