It is noted that supaglutide treatment elicited a general shift to smaller adipocyte sizes in all adipose depots detected, including BAT, inguinal WAT as well as epididymal WAT. To certain extent, the size of adipocyte generally reflects the metabolic function of adipocytes (Skurk et al., 2007). Although, it is not clear which absolute range of adipocytes sizes is metabolically harmful (Verhoef et al., 2013), it is generally accepted that a conversion of small adipocytes to large ones is closely related to common, health risks including hyperlipidemia, diabetes, hypertension and cardiovascular diseases. In a sharp contrast, an increase in the number of small adipocytes promotes lipid metabolism and insulin sensitivity (Spiegelman and Flier, 1996). Our observations that supaglutide decreased adipocyte size in the fat tissues tested were consistent with the improved energy metabolism in these obese mice.