Antibodies used were as follows: ME (1:250, Millipore ab5026, RRID: AB_91644), MOR (1:5,000, Aves Labs), Alexa Fluor 488 anti-rabbit (1:800, ThermoFisher Scientific A21206, RRID: AB_10049650), Alexa Fluor 546 anti-chicken (1:500, ThermoFisher Scientific A11040, RRID: AB_2534097), Alexa Fluor 647 streptavidin (1:2,000, ThermoFisher Scientific S32357), DAPI (1:1,000, ThermoFisher Scientific 62248), biotinylated goat anti-rabbit IgG (1:400, Vector Laboratories BA-1000, RRID: AB_2313606), donkey anti-rabbit conjugated to gold particles (1 nm, 1:50, EMS 25700).