In patient care, combining many of the above techniques will be important. Ideally, clinical angiographic AHO assessment is real-time, includes contributions from the entire AHO pathways, allows for circumferential testing to fairly assess segmentalization and be conducted at physiologic pressures. For surgeries, real-world considerations must be had. While aqueous humor perfusion rigs have become ever sophisticated, patient safety, surgeon comfort, avoidance of unnecessary additional wounds, and time under anesthesia are factors. For example, most surgeons would not be willing to place a sharp needle in the sulcus space of a phakic patient to deliver the tracer there just because it is in a more physiologic starting point compared to the anterior chamber. Like the episcleral fluid wave approach, something familiar and comfortable to the ophthalmic surgeon will be important .