Autophagy is an essential cellular process that helps cells overcome stressful environmental conditions. It is also tightly regulated and requires a large number of proteins to perform correctly. The cooperation and interactions between different regulators are particularly important for the correct execution of autophagy. Autophagy relies intensively on membrane resources for the formation of the autophagosome to sequester the targeted cytosolic components. Therefore, many membrane traffic regulators, including many monomeric GTP-binding proteins, have been shown to be essential for this process. Examples include Ypt1 and Sec4. While Ypt1 normally controls the traffic between the ER and the cis-Golgi (Jedd et al. 1995) and Sec4 is required for the exocytic secretion pathway (Kabcenell et al. 1990), both are required for membrane transport to the PAS to form the autophagosome (Geng et al. 2010) (Lynch-Day et al. 2010).