For the correct functioning of a centromere, it must be flanked by clusters of repeats of 500 kb or more . We suppose that a failure in detection of C-bands in the pericentric region is due to their small sizes or may be due to technical issues with C-banding. In P. khaoyaiensis chromosomes, there are C-bands in distal regions separated by small C-negative regions (Figure 5a). The approach that has been suggested before of producing microdissection-derived probes would allow for estimating the homology of their DNA. In the case of revealed homology, it can be an argument in favor that more proximal C-bands derive from terminal ones by transposition of repeats or by a small inversion. Interestingly, the chromosomes of P. khaoyaiensis are characterized by many C-bands located close to each other.