Acid Phosphatase (ACPP) is one of the oldest biomarkers used for PCa diagnosis in serum, however the drawback of this biomarker is that ACPP is expressed by both normal and malignant prostatic tissues, as well as extraprostatic tissues . Prostate-Specific Antigen (PSA), also known as kallekrein 3 (KLK3) is the most widely used serum biomarker of PCa and has tremendously improved the diagnosis of PCa. PSA screening was widely adopted in the USA in early 1990s as gold standard investigation for PCa . However, recent evidence indicates that PSA falls short in its diagnostic ability in the lower reference ranges (2-10ng/mL). This led to its contraindication in the USA for men greater than 75 years in 2008 ; and for all men in 2012 . Even though highly sensitive, it is not so specific and has led to a high false positive rate, false negatives and overtreatment of PCa patients. Despite this down side to PSA, very few biomarkers are currently poised to replace PSA in clinical practice [20, 21]. To improve the diagnostic ability of PSA, several related parameters such as PSA doubling time, PSA velocity, free-to-total PSA ratio and prostate health index (PHI) have been explored .