Effect of supaglutide on food intake and energy expenditure in the DIO mice. (A) Food intake. Oxygen consumption (VO2) expressed as per mouse in (B) and normalized by body weight (BW) in (C). Carbon dioxide release (VCO2) expressed as per mouse in (D) and normalized by BW in (E). Energy expenditure (EE) expressed as per mouse in (F) and normalized by BW in (G). Significant difference between control and SPG is determined using unpaired Student's t-test. All values are means ± SE (n = 4 for each group). *p < 0.05; **p < 0.01.