CaLC3378: To C terminally HA tag CAS5 in a mutant carrying the PKC1 M850G mutation, the CAS5-HA-HIS cassette was released from pLC790 with ApaI and transformed into SN95 PKC1 M850G /PKC1. Proper integration at the CAS5 locus was verified by amplification using primer pairs oLC2163 in combination with oLC2029 and oLC2164 in combination with oLC1645. Expression of HA-tagged Cas5 was verified by western blot.