Pre-GMP ABY029 uptake was evaluated using an infrared imaging system and the level of fluorescence from rat ex vivo brain slices was acquired at different time points post-injection. Figure 1 presents the 1:1 correlation of whole brain fluorescence and H&E histologic staining. This co-registration demonstrates a high correlation between tumor presence and fluorescence.Fig. 1.Multiple plane co-registration of brain tumor morphology (a, d) and ABY-029 fluorescence (b, c) in a series of brain slices from the same rat. The tumor regions are fairly obvious in the H&E, and are circled in the lower left H&E images, which correlate well with the fluorescence areas. Each row from columns a and b or c and d correspond to the same slice of the brain