In cell attachment test, the cell attachment at time points of 4, 8, 12 and 24 h were 12.5, 50.0, 86.1 and 106.0 %, respectively, as shown in Fig. 9. This result means that MG-63 cells needed at least 24 h in order to attach inside of the PLGA scaffold. Because of applying compressive stimulation on scaffold, the seeded cells should be placed inside of the scaffolds as well as enough number of cells should be attached in/on scaffolds.Fig. 9MG-63 cell attachment rate for 1 day for control and experimental specimens. Optical density at 450 nm (a) and cell number (b) of cell attachment at each time point (n = 4, *p < 0.05)