So far, IAG has been shown to be a key regulator of reproductive processes such as sexual differentiation, spermatogenesis, and sexual shifts in crustaceans. Insulin receptors are pivotal players in insulin signaling pathway (Figure S2), which mediate insulin and ILP signaling . However, apart from characterization of the hormone itself, little information regarding other elements of the signal transduction pathway is available. Unigene10231 is also identified as an insulin-like growth factor-1 receptor in progesterone-mediated oocyte maturation and oocyte meiosis pathways (Figure S3). Oocyte development can be inhibited by the androgenic glands when cultured in vitro. Unigene10231 was significantly downregulated in SP AG and participated in progesterone-mediated oocyte maturation and oocyte meiosis pathways. We can infer that Unigene10231 may indirectly regulate spermatogenesis and male secondary sexual characteristic development. In addition, whether CL1931 participates in sexual differentiation is unclear, which still needs further study.