Immunoblotting was performed as previously described (Wan et al., 2015). Primary antibody Ucp1 (1:500 for white adipose tissues and 1:4,000 for brown adipose tissue) was from Abcam (ab155117), HSP90 (1:1,000) was from Santa Cruz (sc-7947), and GAPDH (1:1,000) was from Biotech Well (WB0197). Horseradish peroxidase-conjugated secondary antibody (1:5,000) was from SAB (anti-Rabbit 3012; anti-Mouse: 3032). Protein band densities were quantified using the Image J program.