Bombykol precursors are stored in the form of TAGs within cytoplasmic LDs. The LDs release the bombykol precursor fatty acid in response to the external PBAN signal. The PGs were stained with Nile Red to monitor the LD dynamics after the successful suppression of GPAT transcript by RNAi. Nile Red staining revealed that PBAN treatment significantly reduced the fluorescence brightness, indicating reduced LD production. The female PGs injected with GPAT dsRNA substantially accumulated fewer LDs compared with the control females injected with EGFP dsRNA (Fig. 5C). Further TAG content analysis manifested that the female PGs injected with GPAT dsRNA synthesized fewer TAGs compared with the control females injected with EGFP dsRNA (Fig. 5D). These results suggested that GPAT significantly affected the biosynthesis of TAGs and LDs. In addition, GPAT-F1 dsRNA had stronger inhibitory effect than GPAT-F2 dsRNA. These results were in agreement with the findings of RNAi efficiency.