Five species of stick insects were maintained and bred as laboratory cultures: Medauroidea extradentata (Phasmatidae) , Sungaya inexpectata (Heteropterygidae) , Sipyloidea sipylus (Diapheromeridae) , Phaenopharos khaoyaiensis (Diapheromeridae) , and Peruphasma schultei (Pseudophasmatidae) . Only P. schultei presented with a bisexual population in our setting. The other four species included exclusively parthenogenetic females. M. extradentata, S. inexpectata, S. sipylus, and P. khaoyaiensis were fed with rasp- and blackberry leaves, P. schultei with privet (Ligustrum) leaves. All of them were kept on their fodder plants up to the cytogenetic analyses.