Although some KTs from W. anomalus display high stability at 37 °C and even higher temperatures , many KTs show lower thermostability . We found that the optimal temperature for Wa1F1-KT activity was lower than the physiological value in the human body. Nevertheless, the absence of β-glucans on mammalian cells suggests its potential application against fungal infections at skin and mucosal membrane levels, as has been demonstrated with other KTs . Further characterization of Wa1F1-KT enzymatic activity and the cloning of its encoding gene may represent the next step to investigate the feasibility to produce molecules with broader therapeutic activity, possibly including systemic infections.