BAECs were irradiated at 10 Gy, followed by pre-treatment with Pazopanib for 16 h. Cells were collected at indicated time after radiation and ASMase activity was measured by quantifying conversion of (14C)sphingomyelin to the product (14C)phosphocholine. The result represents the duplicate determinations from 2 experiments. Data is the mean ± SD. * indicated p < 0.05 (10 Gy vs 10 Gy + Paz) (A). After pre-treatment with Pazopanib for 16 h, BAECs were irradiated at 10 Gy. Total lipids were extracted at the indicated time after radiation, and C16-ceramide level was measured by liquid chromatography, electrospray ionization-tandem mass spectrometry. Data is the representative determination from 2 experiments. Data is the mean ± SD. *p < 0.05 and **p < 0.01; 10 Gy vs 10 Gy + Paz (B).