Metabolic labeling with fluorescent fatty acids is performed in the context of zebrafish development, yolk absorption, and dietary lipid metabolism. Fluorescent fatty acids (BODIPY-FL-C12 depicted) are trafficked and metabolized along with native yolk or dietary lipids when delivered to the developing zebrafish by yolk injection or feeding. LD, cytoplasmic lipid droplet, LP, lipoprotein, VLDL, very low-density lipoprotein. Embryo and larva illustrations adapted from Ref. (18).