For the identification of whole NKT or iNKT cells, the MNCs were stained with anti-TCR αβ Ab (H57-597, eBioscience, San Diego, CA) and either anti-NK1.1 Ab (PK136, eBioscience) or the α-GalCer-loaded CD1d tetramer (MBL, Nagoya, Japan), respectively. Macrophages were identified by staining MNCs with anti-F4/80 (BM8, eBioscience) and anti-CD11b Abs (M1/70, eBioscience), and their expression of TLR-9 was examined using anti-TLR-9 Ab (J15A7, BD Biosciences). B cells were identified by staining MNCs with anti-B220 Ab (RA3-6B2, eBioscience). Flow cytometry was performed on a Cytomics FC500 instrument (Beckman Coulter, Indianapolis, IN).