The 262 MCC and 130 Pakistani varieties were genotyped with the primer pair TaFlo2-InDel8, and PCR product was run on 8% polyacrylamide gels. Based on TaFlo2-InDel8 scoring, the MCC and Pakistani accessions sorted into two groups according to their haplotypes (TaFlo2-A1a or TaFlo2-A1b) for the TaFlo2-A1 gene. For MCC, the average values of TGW of the two haplotype groups were calculated using replicated data collected from 3 years (2002, 2005, 2006) of plants in Beijing . For Pakistani varieties, the average values of TGW of the two haplotype groups were calculated using replicated data from 2 years (2009, 2010) of field trials at the University of Agriculture, Faisalabad. The resulting values were then compared and statistically analyzed using SPSS 13.0 for Windows (IBM, New York, USA).