Nine anti-retroviral naïve HIV-1 subtype C infected pediatric cross-neutralizers were recruited for the generation of the human anti-HIV-1 scFv recombinant library, based on the viral cross neutralizing potential exhibited by their plasma antibodies (35, 36, 38). The demographic and clinical profile of the study subjects is provided in Table 1. The infected donor plasma samples were analyzed for the presence of anti-CD4bs antibodies using recombinant gp120 proteins (13, 53) known to bind CD4bs-directed bnAbs and their corresponding mutants; the RSC3 core protein and its mutant Δ371I/P363N, HXBc2 WT gp120 and its mutant D368R. The plasma antibodies of 4 pediatric donors (AIIMS_341, AIIMS_346, AIIMS_357, and AIIMS_361) demonstrated high binding titers with RSC3 core WT protein as compared to its mutant Δ371I/P363N, suggesting the presence of VRC01 like anti-CD4bs antibodies in their plasma (Figure 1A); this was further confirmed with HXBc2 WT gp120 and its mutant D368R (Figure 1B). The results for the binding of positive and negative control antibodies with RSC3 core WT and its mutant Δ371I/P363N are shown in (Figure 1C) and with HXBc2 WT and its mutant D368R in (Figure 1D).