Oxygen-glucose deprivation (OGD) induces Nrdp1 upregulation in primary rat cerebral cortical neurons and PC12 cells. Cells were subjected to OGD treatment or normoxia (Control, Con) for 1, 3, or 6 h before analyzing Nrdp1 mRNA and protein expression using real-time RT-PCR and western blot. (A) Real time RT-PCR analysis showed that Nrdp1 mRNA expression was significantly increased in primary rat cerebral cortical neurons at 1 h after OGD treatment and was further increased when OGD was prolonged to 6 h. *P < 0.05 vs. Con, ANOVA; n = 4. (B) Western blot analysis showed that Nrdp1 protein levels were increased in 3-h OGD- and 6-h OGD-treated primary rat cerebral cortical neurons, but not in 1-h OGD-treated cells. Upper panel: representative immunoblots of Nrdp1 and the loading control β-actin; bottom panel: quantitative data of protein band intensity after normalization to β-actin. *P < 0.05 vs. Con, ANOVA; n = 4. (C) Real time RT-PCR analysis showed that Nrdp1 mRNA expression was significantly increased in PC12 cells at 3 h and 6 h after OGD treatment, but not 1 h. *P < 0.05 vs. Con, ANOVA; n = 4. (D) Western blot analysis showed that Nrdp1 protein levels were increased in 6-h OGD-treated PC12 cells, but not in 1-h OGD- and 3-h OGD-treated cells. Upper panel: representative immunoblots of Nrdp1 and the loading control β-actin; bottom panel: quantitative data of protein band intensity after normalization to β-actin. *P < 0.05 vs. Con, ANOVA; n = 4.