Ten μg of proteins were loaded in each lane of a 10% Bis-Tris precast gel (NuPAGE, Life technologies) and run in NuPAGE 1x MOPS SDS running buffer (Life Technologies). After migration of the samples, the proteins were transferred on a PVDF membrane (0.45 µm) (Millipore). Membranes were blocked for 1 h in Odyssey blocking buffer, incubated overnight with primary antibodies (mouse TAU-13 or rabbit anti-actin (Abcam), washed three times with PBS 0.1% Tween-20, incubated 1 h at RT with secondary anti-Mouse-IRDye CW-800 or anti-Rabbit-IRDye CW-680 antibodies (Odyssey). The membranes were finally scanned using LICOR (Odyssey Infrared Imager) and quantification performed using the LICOR software.