pLC818: This is a construct to C terminally HA-tag Cas5 in C. albicans. The CAS5 ORF, HA, and HIS1 were amplified from CaLC3113 genomic DNA with primers oLC3365 in combination with oLC3366 and cloned into pLC49 at the SacII site. The ligation mixture was transformed into TOP10 cells. Cells were plated on LB + Amp + NAT. Correct integration of the cassette in pLC49 was sequence verified with oLC2029, oLC244, and oLC3371. This construct can be liberated by digestion with SacII and transformed into C. albicans.