The linkage map was constructed using MultiPoint 3.3 (http://www.multiqtl.com/) as described in (Hirakawa et al., 2016). The complete set of 188 F2 lines of a biparental population 92S80 (cv. Woogenellup x cv. Daliak) phenotypic data as reported by Ghamkhar et al., 2011 was used for QTL screens for the following morphological and agronomic traits: levels of the oestrogenic isoflavones, formononetin (FO), genistein (GT) and biochanin A (BCA); days to first flowering (FT); leaf marks (LM); pigmentation of calyces (CP); hairiness of stems (SH); and hardseededness (HS). Levels of the isoflavones FO, GT and BCA were measured using the technique of Francis and Millington (1965). FT was measured as the number of days from sowing to appearance of the first flower. The morphological traits, LM, CP and SH, were scored 120 days after sowing, using the rating systems given in Nichols et al., (1996). Hardseededness was measured in the laboratory using the method of Quinlivan (1961).