RNA was extracted using the NucleoSpin RNA II kit (Macherey Nagel). cDNA was synthesized using High capacity cDNA RT kit (Life Technologies) according to the manufacturer’s protocol. Real-time PCR was carried out in sealed 384-well microtiter plates using the SYBR™ Green PCR Master Mix (Applied Biosystems), according to Applied Biosystems gene amplification specifications (40 cycles of 15 s at 95 °C and 1 min at 60 °C). The following forward (F) and reverse (R) primers were used for RUNX1: F-RUNX1 (5′-ACAAACCCACCGCAAGTC-3′), R-RUNX1 (5′-CATCTAGTTTCTGCCGATGTCTT-3′); and for ETV6-RUNX1: F-ETV6 (5′-AAGCCCATCAACCTCTCTCA-3′), R-RUNX1 (5′-TCGTGGACGTCTCTAGAAGGA-3′). Data analysis was performed using the ΔΔCT-method . The housekeeping genes GAPDH or ABL were used to normalize the data. The log2 fold change of all genes of interest was calculated compared to Nalm6 control cells.