The expression changes of proteins and phosphoproteins in the hippocampus and cortex of STZ-treated and untreated control mice were quantified and identified on 2-DE gels using Prodigy SameSpot software and MALDI-TOF MS/MS. Image analysis showed approximately 400 protein spots and 200 phosphoprotein spots on each SYPRO Ruby-stained 2-DE gel (Figure 1) and each Pro-Q Diamond-stained 2-DE gel (Figure 2), respectively. We detected 16 (5 up- and 11 downregulated) hippocampal proteins (Table 1), 16 (7 up- and 9 downregulated) cortical proteins (Table 2), 3 (1 up- and 2 downregulated) hippocampal phosphoproteins (Table 3), and 4 (2 up- and 2 downregulated) cortical phosphoproteins (Table 3). These proteins and phosphoproteins were categorized into functional groups as shown in Tables 1–3 using the PANTHER (http://www.pantherdb.org/) database.