Previously, we found glycosylation changes throughout healthy pregnancies, and have in RA patients studied the specific glycosylation of IgG-Fc throughout pregnancy (20, 21, 27, 28, 42, 43). In the current study, the first application of a similar MALDI-MS approach with automated sample preparation, we achieved the analysis of the total serum N-glycosylation throughout the pregnancies of both healthy controls and RA patients, in total leading to the analysis of 1,770 clinical samples (30). Since the current cohort also contains the control individuals (without RA) from prior studies, these previous results were confirmed, but we could also for the first time show that comparable TSNG changes can be observed in RA patients.