Offered commercially plasmid vectors (Tubb2c, Atp6v1a, Pdhb, Hsc70t, Fbp1, Lrrc34, Dnpep, Gapdhs, Pdha2 and Dazap1) were transfected into human embryonic kidney 293 (HEK293) cells. Expression by HEK293 of TGC-specific AI proteins has been confirmed by FLAG antibody (Fig. 7-1). EAO sera (Fig. 7-2) reacted to various TGC proteins, but control sera did not (Fig. 7-3). Presences of Hsc70t (Fig. 7d), Pdha2 (Fig. 7i) and Dazap1 (Fig. 7j) antibody in EAO serum but not in control serum were determined by Western blot. Presences of Tubb2c (Fig. 7a), Atp6v1a (Fig. 7b), Pdhb (Fig. 7c), Fbp1 (Fig. 7e), Lrrc34 (Fig. 7f), Dnpep (Fig. 7g) and Gapdhs (Fig. 7h) antibody in EAO serum and control serum were not determined by Western blot (Fig. 7).