In contrast, Sbp1m only cosediments with the RNP fraction and does not show interactions with the ribosome. This result suggests that the methylation state of the RGG repeats in Sbp1 abolishes its interaction with the ribosome. In addition, because of changes in the protein–protein interaction by methylation, the nature of RNPs formed with Sbp1 and Sbp1m on 5′UTRPab1 is likely to be different.