CsTegu20.6 was searched against an expressed sequence tag (EST) database of C. sinensis developmental stages constructed using large-scale sequencing . The nucleotide sequence was translated using the ExPASY Translate tool (http://web.expasy.org/translate/). The ORF was predicted using the ORF-predictor server . The physico-chemical properties of the predicted protein, including molecular weight and pI, were calculated by ProtParam . Functional domains was searched using InterProScan , signal peptides with SignalP 3.0 , protein subcellular localization using TargetP  and transmembrane regions with TMHMM . Homologous proteins were collected from a BLASTP search (National Center for Biotechnology Information, Bethesda, MD, USA) . Multiple sequence alignments were generated using MAFFT  and were displayed by ESPript .