Nitrogen application significantly increased N uptake under the different planting patterns (Table 3). The N uptake of straw and grain was 7.4% higher and 4.0% lower, respectively, in IM than in MM and 55.3% (significantly) higher and 8.5% lower, respectively, in MS than in IS. Comparing N application rates in MM, the N uptake of grain in CN was greater than that in RN, whereas the straw N uptake in RN was greater than that in CN. In IM, grain and straw N uptake in RN were higher than those in CN. The N uptake of soybean in RN was the highest among all treatments, with N uptake of straw and grain greater in RN than in CN for MS and IS.