After culturing the melanocyte cells in the conditioned mediums containing the fibroblast cell secretions, the foreign medium could potentially have affected melanocytes and melanin production. The alterations in melanin quantities in the melanocytes were confirmed using the melanin content method. Fibroblasts with exposure to UVB, SCF gene knockdown, and SCF gene knockdown with exposure to UVB were compared to figure out the actual variation in melanin content in melanocytes due to the different culturing mediums. In Figure 4a, the melanin production in melanocytes increased by 23.5% when treated in the conditioned medium from SCF fibroblasts knockdown compared to the normal fibroblasts and increased 27.3% under SCF fibroblasts knockdown with UVB exposure. In Figure 4b, the morphology of melanocytes was altered by treatment with the conditioned medium of fibroblasts with SCF knockdown and UVB exposure, resulting in the melanocytes getting more synapses than UVB only. The result was consistent with Figure 2, indicating SCF silencing caused an essential variation in melanin quantities in the melanocytes.