Kidney fragments from WT or CalpTG mouse have been incubated in 1 mg/ml collagenase 1 solution (Gibco, Life technologies) for 3 mn at 37 °C. Tissue was passed in 100 µm and 40 µm sieves to collect renal tubular cells. Cells were grown in a specific medium to promote tubular cells growth and differentiation containing HAM’s F12 and DMEM medium, insulin 5 µg/ml (I1882, Sigma), dexamethasone 5.10−8 M (D8893, Sigma), selenium 60 nM (S913, Sigma), transferrin 5 µg/mL (T1428, Sigma), triiodothyronine 10−9 M (T5516, Sigma), EGF from mouse 10 ng/mL (E4127, Sigma), HEPES 20 mM (15630-056, GIBCO), Glutamine 2 mM (25030024, Gibco), 2% Fetal calf serum (Invitrogen), and 0.5% D-Glucose (Sigma).