Though it was beyond the scope of our recent metabolic labeling study (29), the biochemical techniques described therein could be applied to later-stage larvae in order to examine potential crosstalk between adipose tissue and the enterocytes that could influence dietary lipid partitioning. We have also developed methods for using fluorescent fatty acids as metabolic labels in the context of standard and lipid-enriched diets in larval zebrafish (Table 2). In addition to exploring the metabolic labeling potential of fluorescent lipids whose product profiles were not previously described, we have also applied HPLC with charged aerosol (total lipid detection) and fluorescence detection to obtain a greater depth of information than previous studies using fluorescent TLC (28). Initial findings indicate that the partitioning of saturated fluorescent fatty acids among complex lipid classes varies with carbon chain length, the total fat and cholesterol content of the diet, and the type of fluorescent tag (29). Metabolic labeling with fluorescent fatty acids in the context of lipid metabolism by the larval zebrafish is summarized in Figure 4.