The Ramachandran plot , ProSA , QMEAN , ERRAT  and ModFOLD6  methods were employed to validate the final model of CsTegu20.6. The Ramachandran plot ensured the quality of the final model, indicating that 85.5% of residues were in the favorable region and 11.9% in the additionally allowed region (Figure 3A). Furthermore, there were only 2.5% residues in the disallowed region, and 0% of the residues were located in the generously allowed region. Only Ile19, Ala47, Ala74 and Arg164 were found in the disallowed region of the plot. This plot was used to assess the constructed model for its main chain conformation, illustrating the Φ-Ψ torsion angles for all residues, except for Gly and Pro. The ProSA Z-score of −7.41 confirmed the final model as an extremely good model (Figure 3B), whereas the QMEAN Z-score of −2.33 suggested that the model might be a fairly good representation of the protein (Figure 3C). ProSA analyzed the overall quality score by calculating the atomic coordinates of the model, indicating the Z-score of the experimentally solved structures deposited in PDB , whereas the QMEAN Z-score is a measure of the “degree of nativeness” of a given structure and provides information regarding whether the model is of sufficient quality to be comparable to high-resolution crystal structures of similar size. The ERRAT overall quality score of 80.8 showed that the structure could be considered as a good model (Figure 3D). The ModFOLD6 global model quality score of 0.49 indicated that the final model was confidently folded, and the 3D cartoon of the model was colored by the per-residue error according to the B-factor values (Figure S5).