Here we have demonstrated that following excision from dfr2, Tgm9 induces mutations in unlinked soybean genes (Fig 1). We have also demonstrated from analysis of 105 independent Tgm9-induced mutants that the element transposes to unlinked loci on all 20 soybean chromosomes (Fig 2). Since Tgm9 is the only intact CACTA-type element in T322, the mutant genes or sequences identified in this study were resulted from insertion of Tgm9. To our knowledge, Tgm9 is only reported active transposable element in soybean .