In addition this study analyzes the variations of marker expression among iPSC samples using TRA-1-60 and TRA-1-81 antibody (mouse, 1:100, Chemicon, Billerica, MA, http://www.chemicon.com/). The cells were analysed with a laser scanning confocal microscope equipped with Fluoview SV1000 imaging software (Olympus FV1000, Olympus, Tokyo, Japan). It expressed only on the established healthy iPSCs and not on unhealthy iPSCs which is presented in S1 Fig. Hence the variations of marker expression among healthy and unhealthy colonies and morphology were used to label the iPSC samples used in this study.