In qPCR, the univariate survival analyses revealed that the patients with Lgr5 low expression (p=0.038) and CD133 high expression (p<0.001) had significantly worse prognosis than those with Lgr5 high expression and CD133 low expression, respectively (Figure 8). Contrarily, no significant differences in prognosis were found between the low and high expression of Oct4 (p=0.351), CD54 (p=0.237), EpCAM (P=0.172) and Sox2 (p=0.189) (Figure 8). In multivariate analyses, only CD133 was demonstrated to be independently related to survival outcomes (p<0.001, HR=4.338, 95%CI [2.152-8.747]).