Extraction of (−)-α-bisabolol was performed using four common vegetable oils: canola, olive, corn, and soybean oil. A total of 0.1 g of (−)-α-bisabolol was added to 10 mL of sterile TB medium in a glass vial and 1 mL of individual vegetable oil was overlaid to the solution. After gentle stirring of the mixture, it was incubated at 25 °C for 1 h to separate the water-based TB medium and oil phase. The aqueous layer was removed and the organic layer was transferred to a new vial for gas chromatography (GC) analysis of (−)-α-bisabolol. The recovery yield of each vegetable oil was compared with that of n-dodecane. To determine the recovery efficiency of n-dodecane from cell pellets and TB medium, E. coli DH5α cells transformed with the pSNA-MrBBS-IspA plasmid were cultivated in TB medium in the presence of 20% (v/v) n-dodecane at 30 °C at 180 rpm for 48 h. After centrifugation of the culture at 14,000 rpm for 10 min, the pellet, supernatant, and organic layers of n-dodecane were fractionated. To determine the recovery yield of n-dodecane, the organic layer was directly subjected to GC analysis of (−)-α-bisabolol. The isolated supernatant was mixed with 20% (v/v) n-dodecane, incubated for 30 min, and centrifuged at 14,000 rpm for 10 min. The organic layer was isolated and used for GC analysis of (−)-α-bisabolol. The cell pellet was first disrupted by sonication and the lysate was used for the extraction and quantification of (−)-α-bisabolol through the process described above.