The mean (SD) values for anxiety and depression (HADS) and the eight subscales of the SF-12 are shown in Table 2. There was a main effect for weekday sitting time on anxiety (F(1, 41) = 3.05, p = 0.040, ƞ2 = 0.18), depression (F(1, 41) = 2.91, p = 0.047, ƞ2 = 0.16), mental health (F(1, 41) = 3.50, p = 0.025, ƞ2 = 0.17) and vitality (F(1, 41) = 3.70, p = 0.020, ƞ2 = 0.22). Planned contrasts identified individuals in group 1 had lower anxiety and depression (Fig 1) and higher mental health and vitality scores (Fig 2) than individuals in groups 2 or 3 (p ≤ 0.05). No differences were found between individuals in group 2 and group 3 (p ≤ 0.05). No main effects were found for weekend sitting (p ≥ 0.05) across any of the mental health outcome variables.