We previously investigated AIs relevant to TGC-induced EAO through reacting individual immune serum samples with testes from normal mice of various ages using immunoblotting47. The results showed that the sera obtained from mice with TGC-induced EAO lesions specifically defined testicular antigens with molecular weights of 15 kDa, 40 kDa, 75 kDa, and >200 kDa from 4-week-old mice47. In addition to these, bands of 24–35 kDa and 60–75 kDa were detected in testicular proteins at 8 weeks of age47. Moreover, we incubated immunoblots with EAO serum from various weeks after TGC immunization32. At 0 week of age, a band corresponding to approximately 40 kDa was detected in TGC proteins, indicating the presence of natural autoantibodies against TGC. At 4 weeks of age, in addition to this band, a band of approximately 42 kDa was detected in TGC proteins. At 8 weeks of age, three TGC protein bands (approximately 20, 28, and 60 kDa) were also detected32. Considering the EAO-inducing factors and expression levels in testis at 2 weeks and 8 weeks, the EAO-related proteins were 24–35, 42, and 60–75 kDa. In this study, we identified 11 AIs recognized by serum antibodies from TGC-induced EAO in mice extracted Tubb2c (50 kDa), Atp6v1a (69 kDa), Hsc70t (71 kDa), Fbp1 (37 kDa), Lrrc34 (47 kDa), Gapdhs (48 kDa), Pdha2 (44 kDa), Dazap1 (43 kDa), and the unnamed protein product (55 kDa) from mRNA expression analysis at 2 weeks and 8 weeks of age. Therefore, Atp6v1a, Hsc70t, Fbp1 Lrrc34, Gapdhs, and Dazap1 can reasonably be categorized as EAO-related proteins.