The current study provides the first prevalence estimate of carriage of feline haemoplasma species in Denmark. The prevalence of CMhm and Mhf infections was 14.9 and 1.5% respectively when using RT-PCR. One sample, being positive by RT-PCR, was not identified as CMhm when using conventional PCR. Previous studies have shown a similar prevalence in other European countries [9, 19, 20]. The study was performed by the use of a convenience-sampled cat population. The cats included were examined by a veterinarian and represented both diseased cats and cats attending the clinic for routine health checks. This corresponds to the approach used in other studies [11, 13].