Our next task was to ascertain whether such an interaction occurs in the placenta of pregnancies affected by GDM. A histological analysis indicated that there were numerous neutrophil infiltrates in the villous tissue in GDM placentae (Figure 5A), which was confirmed by fluorescent immunohistochemistry for MPO and citH3 (Figures 5B–D), suggestive of primed and NETing neutrophils (Figures 5A–D).