Not unexpectedly, vast majority of the papers in this Research Topic are related to gene silencing but with totally distinct emphasis. Khalid et al. summarizes the applications of various small RNA based genetic engineering (SRGE) in crop protection, focusing on the technology evolution and successful cases in different crops. Andika et al. reviews the current information on the molecular aspects of antiviral RNA silencing in roots, with emphasis on the interactions between host antiviral defense and soil-borne viruses. The distinctive characteristic features of RNA silencing in roots relative to shoots are summarized. Moon and Park review how the RNA silencing pathway cross-talks with the resistance (R) gene-mediated defense. Several components involved in host RNA silencing mechanisms have recently been shown to be required for R gene-mediated defense. It seems that it is a common phenomenon that miRNAs or siRNAs regulate R-gene mediated resistance through targeting R genes for cleavage in plants (Moon and Park). It is plausible that the cross-talk between these two defense pathways is to maximize the efficiency of defense responses against viral infections (Nakahara and Masuta, 2014). Huang et al. summarize the various scenarios of host- and pathogen-derived sRNAs or pathogen-induced host sRNAs in regulating host resistance/susceptibility or pathogen virulence/pathogenicity.