Obtain genomic DNA by tail fin clipping from the clutch-mate fish of the fish used for homology arm cloning. Perform DNA sequencing and identify several male and female fish whose DNA sequence matches the cloned homology arms. Use these fish to produce embryos for the injection of the pZwtich construct. Inject approximately 50 pg each of pZwtich construct DNA and TALEN mRNAs into one-cell–stage embryos.