The dsRNA corresponding to two regions of GPAT (GPAT-F1 and GPAT-F2) was synthesized to elucidate the function of GPAT (Fig. 5A). When 15 μg dsRNA of GPAT was injected into female pupa 48 h before eclosion, the mRNA level of GPAT in PGs significant decreased 48 h after injection, compared with that in pupa injected with control EGFP dsRNA (Fig. 5B). GPAT-F1 dsRNA had higher interference efficiency than GPAT-F2 dsRNA.