When comparing preexpanded and cells expanded in DMSO, total Treg cells showed a level of TSDR demethylation (median, 25.2% retain) and Foxp3 expression (median, 87.4% retain) that was intermediate to naive Treg and CD25int mTreg subpopulations (data not shown) to manifest an enhanced ratio of FOXP3 expression to TSDR demethylation (Figure 3D), that is probably due to inclusion of CD25int mTreg when sorted on day 0.