Representative spectra for the Formulation Buffer (∙∙∙∙∙∙) and NISTmAb RM 8671 (∙ − ∙ − ∙ − ∙) can be seen in Fig. 1. Figure 1 also depicts a representative blank-subtracted (corrected) NISTmAb spectrum (───). This spectrum displays decadic attenuance characteristic of a protein, with an apex of absorption at ≈280 nm.Fig. 1Representative spectra obtained for the Formulation Buffer blank (∙∙∙∙∙∙), NISTmAb RM 8671 14HB-D-002 (∙ − ∙ − ∙ − ∙), and the blank-subtracted (corrected) NISTmAb spectrum (───)