5) Figure 2A, it would be good to include a control where Mcm10 is incubated with a fork in the absence of CMG. Also, one caveat of this experiment is that Mcm10 is a single-stranded DNA binding protein and the stimulation of the DNA unwinding activity might simply be due to single-stranded DNA trapping (effectively the same role of a DNA capture strand). It would be good to test whether another single-stranded DNA binding protein (e.g. SSB) has a similar effect on DNA unwinding by the CMG. Also, what is the supershifted band visible e.g. in lanes 5, 8 and 11? It would be good to acknowledge this band, since it is present in many unwinding gels throughout the paper.