Kidneys, heart, aorta, skin and spleen from mice of each group were immersed in AFA and formalin (formaldehyde 4%) solution or snap-frozen. After 24 h in formalin solution, brains have been immersed in 20% sucrose solution for 24 hours and then frozen at −80 °C. Formalin-fixed tissues were embedded in paraffin after conventional processing (alcohol dehydration), and 4-µm thick sections were stained with Masson trichromic solution, hematoxylin-eosin or sirius red in picric acid solution. Heart nuclei were stained with DAPI (Invitrogen, Thermofischer scientific, 1/2000) and cell count/surface was performed to assess myocardic hypertrophy by using an Image J software-based Macro. Perpendicular cross-sections at standardized distance from the renal hilus (0.3 mm) have been performed to measure kidney interlobar arteries media surface. Similarly, four sections of the thoracic descending aorta have been performed to assess the mean media surface for each animal. The number of glomeruli/field, the surface of all glomeruli, and urinary chamber have been measured in 10 renal cortex Masson-stained sections at 200x magnification. For each animal, the mean value has been considered. Kidney fibrosis has been assessed by measuring sirius red-stained surface under polarized light in 10 renal cortex sections at 200x magnification. For each animal, the mean value has been considered. All measures have been performed by using AnalySIS® software.