Effect of GK knockdown on feeding behavior in control and GK knockdown animals. (A–F) Food intake (A), first meal duration (B), frequency of eating events (C), cumulative eating time (D), intervals and interval duration (F) were evaluated in unoperated, Ad-EGFP-injected (Ad-EGFP) and Ad-shGK-injected animals (Ad-shGK). Schematic representation of the experimental procedure is shown in the top. Statistics: non-parametric ANOVA; **p < 0.01.