(A) Live cell imaging of LX2 cells transfected with ER-reporter RFP-KDEL plasmid and subsequently stained with ER-Tr. (B) Immunostaining of LX2 cells for the endoplasmic reticulum marker calreticulin; cells were counter-labeled with NRB-AF12. Magnification 60x; scale bar 10 μm. (C) Quantification of ER-Tr co-localization with RFP-KDEL and Calreticulin is shown as Pearson's coefficient (n ≥ 10).