CaLC3189: To introduce a mutant allele of CAS5 carrying the S769A mutation, the CAS5 S769A-HA-HIS1cassette was released from pLC800 with ApaI and transformed into CaLC2034. Transformants prototrophic for histidine were PCR tested for integration of the cassette as described for CaLC3209. The S769A mutation was sequence verified with oLC2029.