In IHC, the patients were divided into Oct4 low (n=266, 70.6%) and high (n=111, 29.4%) expression groups. Oct4 high expression was significantly related to age >60 years (p=0.020), male (p=0.020), macroscopic type III-IV (p<0.001), tumor size >7cm (p=0.023), N3 stage (p=0.003), M1 stage (p=0.011) and TNM III-IV stage (p=0.001). Multivariate analysis showed that gender (p=0.014, OR=0.487, 95%CI [0.275-0.864]) and macroscopic type (p<0.001, OR=1.559, 95%CI [1.228-1.979]) were independent related factors to Oct4 expression.