Nucleotide sequence analysis of actin sequences showed 33 polymorphic sites, three of which caused amino acid substitution. Two of these amino acid substitutions have been previously reported to occur in genotypes G, N, I and P, in which nucleotide 371 substituted alanine for valine, and nucleotide 904 substituted lysine for glutamine [20, 34]. A unique polymorphism leading to an amino acid substitution, in which nucleotide 892 substituted threonine for serine, was observed to be exclusively present on a GenBank sequence (accession number XM_001301892). As such, in silico genotyping of isolates provides an opportunity to distinguish closely related isolates based on these polymorphic sites and to further identify such polymorphic sites.