Expression of miR-484 and miR-744 - Vero cells were seeded at a final concentration of 1.5 × 105 cells/well in 24-well plates and transiently transfected with pGUD1, pGUD2, or pGUD4, at a final concentration of 0.5 µg/well, using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions. Alternatively, Vero cells were infected with each DENV serotype at an MOI of 3. In both cases, the cells were harvested at 8, 16, 24, 32, 48, and 72 hpi and total RNA extraction was performed using the Trizol reagent (Invitrogen) following the manufacturer’s instructions. The RNA concentration was measured using a NanoDrop spectrophotometer (Nano Drop Technologies, CA, USA). Reverse transcription of endogenous miR-484 and miR-744 in transfected and DENV-infected Vero cells was carried out with 10 ng RNA to produce cDNA using the TaqMan® MicroRNA Reverse Transcription Kit (Applied Biosystems, CA, USA). For miRNA reverse transcriptase quantitative-PCR (RT-qPCR), experiments were carried out using the Taqman microRNA Assay (Applied Biosystems) and TaqMan® Universal Master Mix II (Applied Biosystems), according to manufacturer’s instructions, and performed in triplicate on a Bio-Rad CFX96 real-time Detection System (Bio-Rad, CA, USA). The expression of both miRNAs was normalised to 18S rRNA, and the expression levels were determined via the comparative threshold cycle (Ct) method using 2-ΔΔCt. Three independent replicates were performed for each experiment.