Three technical replicates with respect to a Q Exactive mass spectrometer coupled to Easy nLC (Proxeon Biosystems, now Thermo Fisher Scientific) were set for better coverage of the target proteome with reliable statistical consistency. A 10 μL aliquot of each fraction was injected for nanoLC-MS/MS analysis. 5 μg of the peptide mixture was loaded into a the C18-reversed phase column (Zorbax 300SB-C18 peptide traps, Agilent Technologies, Wilmington, DE, 150 mm × 75 μm) packed with RP-C18 5 μm resin equilibrated for 20 min in buffer A (0.1% formic acid in water). The separation of peptide mixtures was performed with a linear gradient of buffer B (80% acetonitrile and 0.l% formic acid) at 250 nL/min rate controlled by IntelliFlow technology over 140 min. the gradient included 4% to 28% (v/v) for 110 min, 28% to 40% (v/v) for 20 min, 40% to 90% (v/v) for 5 min and 90% (v/v) for 5 min.