The 16S rRNA gene from isolate MCCB 248 was PCR-amplified, sequenced, and a phylogenetic tree was constructed. Briefly, DNA was extracted from a 48 h broth culture of strain MCCB using the PureLink Genomic DNA mini kit (Invitrogen, USA). PCR amplification of the 16S rRNA was performed using universal primers 16S1 (GAGTTTGATCCTGGCTCA) and 16S2 (ACGGCTACCTTGTTACGACTT). The amplified PCR product was purified by Exosap (Affymetrix, USA) and sequenced. Nucleotide sequence data were analyzed using BLAST and deposited in NCBI GenBank. The nucleotide sequences were aligned using Clustal W on MEGA 6 (Tamura et al. 2013). The 16S rRNA sequences of closely related actinomycetes were retrieved from GenBank, and their similarity to the present isolate was assessed at the nucleotide level. A phylogenetic tree was constructed using the neighbor-joining method with boot strap values based on 1000 replicates.