Expression and identification of rCsGRN. a rCsGRN was identified by 12% SDS-PAGE. Protein molecular weight markers (Lane M), lysate of E. coli containing pET-28a (+) without induction (Lane 1) and with induction by IPTG (Lane 2), lysate of E. coli containing pET-28a (+)-CsGRN without induction (Lane 3) and with induction by IPTG (Lane 4), supernatant (Lane 5) and precipitate (Lane 6) of the lysate of E. coli containing the recombinant plasmid after induction, the purified recombinant CsGRN protein (Lane 7). b rCsGRN protein was identified as a component of CsESPs. rCsGRN protein was probed by mouse anti-His-tag serum, rat anti-CsGRN serum, rat anti-CsESPs serum and naïve serum (Lanes 1–4, respectively). In addition, CsESPs were probed with rat anti-CsGRN serum and naïve serum (Lanes 5, 6). Protein molecular weight markers (Lane M)