Despite the interest of a prolonged study to get deeper insight the interplay between Nutrition and cochlear aging, the continuity of this long-term nutritional treatment was precluded by the presence of megaloblastic anemia. This fact was described in previous studies analyzing low intakes of folate or vitamin B12 for extended periods of time (Cox et al., 1961; Bills et al., 1992), but was not evident in C57BL/6J mice after 2 months of diet (Martinez-Vega et al., 2015a). Altogether, our data support the role of folate deficiency in premature development of hearing loss, although new experiments aimed to analyze its impact in strains with delayed onset of this sensory impairment should consider beginning nutritional interventions at later stages of animal development. Moreover, studies on the putative value of folate supplementation against hearing loss are needed, as well as the analysis of the incidence of this sensory decline in human populations living in countries with mandatory folate fortification for which only preliminary data are available: http://www.cdc.gov/ncbddd/hearingloss/data.html. In this line, nutrigenomic studies may render clues about the interaction between folate deficiency and the cochlear genome.