Adenyl cyclases (ACs) can convert adenosine triphosphate (ATP) into cyclic adenosine monophosphate (cAMP), which is necessary for the activation of PKA. The IPA analysis revealed that the signal pathway Gβγ/AC2/4/cAMP/PKA was inhibited by VOSL or CD. AC2 is distributed in brain and lung tissues, however, AC4 is widely distributed in various tissues. Therefore, we determined the levels of AC4 (Fig. 5A–C) and its catalysate cAMP (Fig. 5D–F). The results showed that the levels of AC4 and cAMP were decreased dose-dependently in the four test groups compared with the control group, and the levels of AC4 and cAMP in the CD and VOSL treated groups were obviously lower than those in the Cos and Dehy treated groups.