Using enucleated pig and human eyes, aqueous angiography demonstrated segmental AHO patterns that mimicked non-real-time methods. Angiographic signal was validated as representing true AHO using anterior segment OCT and fluorescent dextrans studies . Angiographically positive but not negative regions demonstrated intrascleral lumens compatible with AHO on anterior segment OCT . With fluorescent dextrans, angiographically positive but not negative regions demonstrated trapping of the tracer in AHO pathways in the TM and angle . Quantitative methods have been developed to analyze these types of images .