The activated splenic lymphocytes play an important role in the innate and adaptive immuneresponses by producing cytokines . Cytokines are important in cell–cell communication in the immune system and play key roles in improving the body’s defense mechanism. The T helper (Th) cells are divided into Th1 and Th2 according to the function and their difference in secretion of cytokines. Th1 cells secrete IL-2, IL-12, IFN-γ, and TNF-α, participating in cell-mediated immune responses, while Th2 cells secrete IL-4, IL-5, IL-6, and IL-10, which promote humoral or allergic responses. IL-2, originally described as T-cell growth factor, stimulates the proliferation and differentiation of T cells and increases the IFN-γ secretion of NK cells . IFN-γ is a pro-inflammatory cytokine endowed with potential immunomodulatory effects on Th1 cells differentiation and macrophage activation to acquire microbicidal and antiviral effector functions . IL-4 is a critical participant in allergic inflammation. It induces the differentiation of Th cells into Th2 cells and the growth of B cells . TNF-α, discovered by its antitumor activity, acts as a host defence factor in immunologic and inflammatory responses . The present results showed that cytokines (IL-2, TNF-α, IL-4 and IFN-γ) expression in the AHG-treated group was much higher than that in CY-treated group and high-dose AHG-treat (10 mg/kg) could secrete more cytokines (IL-2, TNF-α, IL-4 and IFN-γ) compared with normal control, suggesting that AHG can reversed the splenocytes function reduced by CY. In addition, AHG can active Th1 and Th2 cell at the same time.