Additional effects of the dietary treatment were analyzed in blood samples obtained at the start and the endpoint of the study (Figure 1B). No differences in serum folate levels between groups were detected at the beginning of the experiment (NF-2M vs. FD-2M), whereas a five-fold decrease was measured in the FD-10M group. This decrease correlated with a seven-fold increase in tHcy levels in FD-10M mice (Figure 1B), as expected for the role of folate in Hcy remethylation. Altogether, these systemic metabolic changes confirmed the efficacy of the dietary treatment.