ELISPOT and chemokines receptors analysis were determined using nonparametric two‐tailed Mann–Whitney (Graph Pad Prism ver. 5.0). Multifunctional analysis of cytokine frequencies was performed using Boolean gating in FlowJoX ver. 10.3 and GraphPad Prism ver. 6.0. To compare the frequency of the multifunctional populations among the groups Mann–Whitney test was used. The differences of variables among groups were considered significant when p < 0.05.