Using serum autoantibodies from mice immunized with only syngeneic TGCs alone, we have identified 11 proteins as respective testicular AIs. Real-time RT-PCR analysis showed that the mRNA expressions of seven TGC-specific AIs (Tubb2c, Atp6v1a, Hsc70t, Fbp1, Lrrc34, Gapdhs, Dazap1) were significantly higher in only mature testis compared to other organs. Three TGC-specific AIs (Hsc70t, Lrrc34, Dazap1) were shown as EAO-related proteins in previous reports and the present study also. Seven TGC-specific AIs (including Tubb2c, Atp6v1a, Hsc70t, Fbp1, Lrrc34, Gapdhs and Dazap1) show human homology. Finally, the recombinant proteins of identified 10 (except unnamed protein) TGC-specific AIs were created by using human embryonic kidney 293 (HEK293) cells and these antigencities were reconfirmed by Western blot using EAO serum reaction. These results indicated Atp6v1a, Hsc70t, Fbp1 and Dazap1 were candidates for TGC-specific AIs. Information of AIs have also received considerable attention because of their role as cancer/testis antigens (CTs). Identification of these AIs will facilitate new approaches for understanding infertility and cancer pathogenesis and may provide a basis for the development of novel therapies. In the future, we are planning to generate some recombinant proteins of TGC-specific AIs.