Genes encoding translation initiation factor eIF1, eIF1A, Pab1, Sbp1 and individual domains of Sbp1 and Pab1 were amplified from the genomic DNA of S. cerevisiae and cloned into the pETNKI-GST-LIC-Amp or pETNKI-His6-LIC-Amp vectors (Luna-Vargas et al. 2011). The type I arginine methyltransferase (HMT1) gene from S. cerevisiae and the eIF2 β and γ subunits were cloned into the pETNKI-His6-LIC-Kan vector. eIF2α was cloned into the pET22b vector. All the pETNKI vectors are generous gifts from Dr. Patrick Celie at the Netherlands Cancer Institute Protein Facility.