Mice were euthanized following orbital exsanguination in deep anesthesia with isoflurane and vital organs were removed as described [12, 36]. The research under IACUC protocol 2014–11635 was first approved Oct. 22 2014 by the Yale Institutional Animal Care and Use Committee (IACUC), was renewed Sept. 7 2016, and is valid through Sept. 30 2017. Yale University has an approved Animal Welfare Assurance (#A3230-01) on file with the NIH Office of Laboratory Animal Welfare. The Assurance was approved May 5, 2015.