Total RNA was extracted from isolated mouse brains using TRIzol following the manufacturer’s protocol. Complementary DNA was synthesized by using the PrimeScript Reverse Transcriptase. Real-time PCR was performed in four repeats for each sample by using SYBR Green PCR mixture with the Bio-Rad CFX96 real-time PCR system as previously described . Relative mRNA levels were analyzed by Bio-Rad CFX Manager. Transcripts of targeted genes were normalized to these of mouse 18s ribosomal RNA gene in the same samples. Primers for 18s ribosomal RNA were 5′- CGGACACGGACAGGATTGACA-3′ and 5′- CCAGACAAATCGCTCCACCAACTA-3′ with a 94-bp PCR product. Primers for mouse gene EGFR and transgene dnEGFR were 5′- TCCTGCCAGAATGTGAGCAG-3′ and 5′- ACGAGCTCTCTCTCTTGAAG-3′ with a 500-bp PCR product. Primers for mouse gene IL-6 were 5′- GGGACTGATGCTGGTGACAACC-3′ and 5′- CATGTGTAATTAAGCCTCCGACTTGTG-3′ with a 128- bp PCR product. Primers for mouse gene IL-1Î² were 5′- GGCAGGCAGTATCACTCATTGTG-3′ and 5′- TGTCCTCATCCTGGAAGGTCC-3′ with an 84- bp PCR product. Primers for mouse gene CCL2 were 5′- TCACCTGCTGCTACTCATTC-3′ and 5′- GTAGGTTCTGATCTCATTTGGTTCC-3′ with a 205-bp PCR product. Primers for mouse gene CXCL10 were 5′- GTCTGAGTGGGACTCAAGGGATCCC-3′ and 5′- CATCGTGGCAATGATCTCAACACGT-3′ with a 155- bp PCR product. Primers for mouse gene TGF-Î²2 were 5′- TGCTTCGAATCTGGTGAAGGCA-3′ and 5′- GGAGAGCCATTCACCCTCCGCT-3′ with a 181- bp PCR product. Primers for mouse gene CNTF were 5′- TTTCGCAGAGCAATCACC-3′ and 5′- AATTGTGACAGGCATCC-3′ with a 433-bp PCR product. Primers for mouse gene H2-D1 were 5′-TCCGAGATTGTAAAGCGTGAAGA-3′ and 5′- ACAGGGCAGTGCAGGGATAG-3′ with a 204-bp PCR product. Primers for mouse gene Serping 1 were 5′- ACAGCCCCCTCTGAATTCTT-3′ and 5′- GGATGCTCTCCAAGTTGCTC-3′ with a 299-bp PCR product. Primers for mouse gene H2-T23 were 5′- GGACCGCGAATGACATAGC-3′ and 5′- GCACCTCAGGGTGACTTCAT-3′ with a 212-bp PCR product. Primers for mouse gene Ggta1 were 5′- GTGAACAGCATGAGGGGTTT-3′ and 5′- GTTTTGTTGCCTCTGGGTGT-3′ with a 115-bp PCR product. Primers for mouse gene Iigp1 were 5′- GGGGCAATAGCTCATTGGTA-3′ and 5′- ACCTCGAAGACATCCCCTTT-3′ with a 104-bp PCR product. Primers for mouse gene Gbp2 were 5′- GGGGTCACTGTCTGACCACT-3′ and 5′- GGGAAACCTGGGATGAGATT-3′ with a 285- bp PCR product. Primers for mouse gene Fkbp5 were 5′- TATGCTTATGGCTCGGCTGG-3′ and 5′- CAGCCTTCCAGGTGGACTTT-3′ with a 194-bp PCR product. Primers for mouse gene Psmb8 were 5′- CAGTCCTGAAGAGGCCTACG-3′ and 5′- CACTTTCACCCAACCGTCTT-3′ with a 121- bp PCR product. Primers for mouse gene Srgn were 5′- GCAAGGTTATCCTGCTCGGA-3′ and 5′- TGGGAGGGCCGATGTTATTG-3′ with a 134- bp PCR product. Primers for mouse gene Amigo2 were 5′- GAGGCGACCATAATGTCGTT-3′ and 5′- GCATCCAACAGTCCGATTCT-3′ with a 263-bp PCR product. Primers for mouse gene Clcf1 were 5′- CTTCAATCCTCCTCGACTGG-3′ and 5′- TACGTCGGAGTTCAGCTGTG-3′ with a 176-bp PCR product. Primers for mouse gene Ptx3 were 5′- AACAAGCTCTGTTGCCCATT-3′ and 5′- TCCCAAATGGAACATTGGAT-3′ with a 147-bp PCR product. Primers for mouse gene S100a10 were 5′- CCTCTGGCTGTGGACAAAAT-3′ and 5′- CTGCTCACAAGAAGCAGTGG-3′ with a 238-bp PCR product. Primers for mouse gene Sphk1 were 5′- GATGCATGAGGTGGTGAATG-3′ and 5′- TGCTCGTACCCAGCATAGTG-3′ with a 135-bp PCR product. Primers for mouse gene Cd109 were 5′- CACAGTCGGGAGCCCTAAAG-3′ and 5′- GCAGCGATTTCGATGTCCAC-3′ with a 147- bp PCR product. Primers for mouse gene Ptgs2 were 5′- GCTGTACAAGCAGTGGCAAA-3′ and 5′- CCCCAAAGATAGCATCTGGA-3′ with a 232-bp PCR product. Primers for mouse gene Emp1 were 5′- GAGACACTGGCCAGAAAAGC-3′ and 5′- TAAAAGGCAAGGGAATGCAC-3′ with a 183- bp PCR product. Primers for mouse gene Slc10a6 were 5′- GCTTCGGTGGTATGATGCTT-3′ and 5′- CCACAGGCTTTTCTGGTGAT-3′ with a 217- bp PCR product. Primers for mouse gene Tm4sf1 were 5′- GCCCAAGCATATTGTGGAGT-3′ and 5′- AGGGTAGGATGTGGCACAAG-3′ with a 258-bp PCR product. Primers for mouse gene B3gnt5 were 5′- CGTGGGGCAATGAGAACTAT-3′ and 5′- CCCAGCTGAACTGAAGAAGG-3′ with a 207- bp PCR product. Primers for mouse gene Cd14 were 5′- GGACTGATCTCAGCCCTCTG-3′ and 5′- GCTTCAGCCCAGTGAAAGAC-3′ with a 232- bp PCR product. Primers for mouse GFAP variant 1 gene were 5′- GACTATCGCCGCCAACTGCA-3′ and 5′- CTAAGGGAGAGCTGGCAGGG-3′ with a 447-bp PCR product. Primers for mouse GFAP variant 2 gene were 5′- GACTATCGCCGCCAACTGCA-3′ and 5′- TCACATCACCACGTCCTTGT-3′ with a 453-bp PCR product.