The assemblies were distinct from genomes of vaccine reference strains Tohama I (GlaxoSmithKline, accession no. CP010964), CS (China, accession no. CP010963), and 137 (Brazil, accession no. CP010323), which have been sequenced previously (10, 11). B202 and B203 were not related, and their genomes differed from that of Tohama I by multiple rearrangements, as well as 186 and 410 single-nucleotide polymorphisms (SNPs), respectively. The genome of B202 was phylogenetically and structurally similar, but not identical, to other strains with the profile prn1-ptxP1-ptxA2-ptxB2-fimH1, such as clinical isolate H375 (accession no. CP010961) (10). B203 appeared to be closely related to Brazilian vaccine strain 137, sharing allele profile prn7-ptxP2-ptxA4-ptxB2-fimH1, but differed by 13 SNPs and a single ~74-kb inversion flanked by rRNA operon copies.