Single-nucleotide polymorphisms (SNPs) are the most abundant type of molecular marker. Accurate and reliable methods have been developed to perform high-throughput genotyping based on SNPs12. With the development of new sequencing technologies, increasing numbers of SNPs have been discovered in wheat1, 2, 13–15. Cavanagh et al.12 released a hexaploid wheat consensus genetic map with 7504 SNP markers from the Wheat9K SNP array using a combination of seven mapping populations. Wang et al.16 mapped 46977 SNPs from the Wheat90K array to the hexaploid wheat genetic map using a combination of eight mapping populations. Using both Wheat9K and Wheat90K arrays, Maccaferri et al.17 released a high-density tetraploid wheat consensus genetic map with 30144 markers (including 26626 SNPs and 791 SSRs) by integrating 13 data sets from independent biparental mapping populations. Recently, Winfield et al.18 documented a hexaploid wheat consensus map with 56 505 SNP markers from the Wheat820K array, spanning 3739 cM in length, using three independent biparental populations. However, although a high-density hexaploid wheat genetic map (>100 000 markers) based on an individual biparental mapping population would be valuable for further genetic research, such as high-resolution mapping and map-based cloning of a targeted major quantitative trait locus (QTL), no such map has been released.