The mean times taken to complete the maze across all three attempts within the same day, at each age, are shown in Fig. 3 and the P-value summary is reported in Table 1. There was no significant (P > 0.05) difference in spatial learning between the Control and GnRHa-Rec groups, at any age and no significant (P > 0.05) interaction between treatment and age. Significant improvement in traverse times over the three attempts were seen at 41 (P < 0.01), 83 (P < 0.001) and 95 (P = 0.050) weeks of age, but at 83 weeks of age the improvement (P < 0.001) was greater in the new maze layout with a 29.7% decrease in traverse times from the first to second attempt, and 10.7% from the second to third attempt.