Phomopsis cane and leaf spot of grapevine (“Phomopsis”) is caused by Diaporthe ampelina (Ascomycota, Diaporthales; syn. = Phomopsis viticola,) (Gomes et al. 2013; Wilcox et al. 2015). Most Diaporthe species are considered hemibiotrophic (Udayanga et al. 2011), with an initial biotrophic phase of plant tissue colonization before the necrotrophic phase, when lesions or cankers develop. In grapevine, leaf and cane infections by D. ampelina are initiated by rain-splashed conidia released from pycnidia present on previously infected tissues. Dispersed conidia adhere to the plant tissues and under suitable conditions, germinate and penetrate tissues through stomatal pores or wounds (Pine 1959). Leaf and cane infections require a minimum of 7 h wetness duration at optimum temperatures of 16–20 °C (Erincik et al. 2003). In plant tissues, the mycelium germinating from conidia invades the cortical parenchyma and forms pseudo-parenchymatous mats among host cells. Host cells become necrotic and shoot lesions and leaf spots usually appear 3–4 weeks after infection (Wilcox et al. 2015). New pycnidia form on these necrotic lesions, providing inoculum for new infections. Lesions remain after lignification in dormant canes, resulting in shoot breakage. On clusters, Phomopsis can cause lesions on the rachis, resulting in loss of up to 30% of yields (Anco et al. 2012).