Further, in our dataset, we saw that animal strain is an important factor in both d-galactose-induced NBS and neurochemical outcome impairment, where the LACA strain showed the highest impairment in NBS. Experiments using Swiss albino showed the largest effect on SOD, those using Balb/c the largest effects on GSH-px and MDA levels. Also, the different number of animals used in each group might affect the results as studies with a small number of animals giving imprecise results.