In order to assess the development and progression of NAFLD, qCT scans were used to quantify liver density as an indirect measure of hepatic fat content. Liver qCT-scans were performed after 8 and 16 weeks in isoflurane-anesthetized rats using a Latheta CT-scanner (LCT-200 series, Aloka co. LTD, Tokyo, Japan). Changes in liver density were calculated by subtracting baseline qCT values from week 8 and 16 time point values.