The regulatory framework in Mexico enabled field characterization of GM maize hybrids resistant to lepidopteran and/or coleopteran insects and tolerant to glyphosate herbicide in multiple locations of Northern Mexico in order to inform the ERA. The agronomic and phenotypic characteristics measured in 36 studies conducted across agricultural regions during 2009–2013 demonstrated that MON-89Ø34-3 × MON-88Ø17-3, MON-89Ø34-3 × MON-ØØ6Ø3-6, and MON-ØØ6Ø3-6 GM maize hybrids were not different from conventional maize hybrids in potential risks of weediness, pest potential, competition, or displacement. Thus, it was demonstrated that introgression of GM traits into the maize crop did not cause unexpected modifications to the plant or changes in the crop that would suggest changes in pest potential. Together, these results and crop management considerations to reduce gene flow (Baltazar et al. 2015) strongly support the conclusion that commercial plantings of GM maize hybrids would not increase potential environmental risks for cultivation and conservation of maize in Mexico. Furthermore, the results from these studies indicate that MON-89Ø34-3 × MON-88Ø17-3, MON-89Ø34-3 × MON-ØØ6Ø3-6, and MON-ØØ6Ø3-6 GM hybrids are valuable options for integrated crop production systems that can increase productivity per unit area, provide economic gains to Mexican farmers through reduction of production costs for pest control (insects and/or weeds), and benefit the environment.