Blood samples were centrifuged at 2,000 g for 10 min to obtain serum. Triglyceride (TG), Total cholesterol (TC), high density lipoprotein (HDL), low-density lipoprotein (LDL), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels were analyzed using Zhuoyue-450 automatic biochemical analysis device (Shanghai Kehua Biotechnology, China). Nonesterified fatty acids (NEFA) levels were determined using the LabAssay™ NEFA kit (WAKO, Japan).