To test for Drp1/adaptor interactions, cells were chemically cross‐linked using dithiobis (succinimidyl propionate) (DSP; Thermo Fisher Scientific) at a final concentration of 1.0 mM in PBS. After incubation for 1 h at room temperature, cross‐linking was stopped by addition of Tris HCl (pH 7.7) to a final concentration of 20 mM. The cells were lysed with a lysis buffer containing 20 mM Tris–HCl, 150 mM NaCl, 10 mM EGTA, 10% glycerol, and 1% Triton X‐100 (pH 7.4), and immunoprecipitation was performed using either Fis1 or Drp1 Abs and Pierce™ Protein A/G Magnetic Beads (Thermo Fisher Scientific) for 16 h at 4°C. The samples were washed three times in lysis buffer and boiled in SDS sample buffer containing 5% 2‐mercaptoethanol to cleave the cross‐linking. Samples were loaded on SDS–PAGE gel, transferred on to nitrocellulose membrane, and probe with the indicated antibodies.