Chromatin preparation and immunoprecipitation against PolII using 2 µl of 8WG16 antibody were performed as described previously65. Multiplex sequencing libraries were constructed according to Wong et al.66 ChIP-Seq libraries were assessed by Bioanalyzer DNA High Sensitivity Assay and quantified using real-time PCR before sequencing on the Illumina HiSeq2500 platform. Raw reads were mapped to the C. albicans reference genome. Mapped reads on gene bodies were counted, normalized to total number of mapped reads and expressed as “Normalized PolII ChIP-Seq signal”. Differentially bound genes are defined by greater than 1.5-fold difference in PolII ChIP-Seq signals between any two conditions analyzed. Non-expressed genes or lowly expressed genes (e.g., genes with PolII ChIP-Seq signals lower than 10) under both conditions analyzed were removed from downstream analysis. Pathway analysis was carried out using the GO program on the Candida Genome Database (http://www.candidagenome.org/cgi-bin/GO/goTermFinder) and the KEGG mapper (http://www.genome.jp/kegg/tool/map_pathway2.html).