To explore the physiological relevance of the above findings, we inhibited endogenous miR-20a expression by LNA-20a. We observed that LNA-20a remarkably increased LC3 puncta formation under nutrient starvation (Figure 4a and Supplementary Figure S2b). Additionally, immunoblotting showed that LNA-20a enhanced the ratios of membrane-bound lipidated LC3-II in both MDA-MB-231 and MCF7 cells (Figure 4b).