1) Over-interpretation of lower-resolution parts of the map in terms of the atomic model. Although the new supplementary figures now reveal the limitations of the map more clearly, over-interpretation in the text still remains a problem. Specifically, W433 is mentioned to move wrt the crystal structure, yet there is no density visible for W433 in Video-1, Figure 2—figure supplement 2 or 3 to support this statement. The map in this region is ~6Å. The same can be said for H407 that does also not have clear density. The authors should stay away from over-interpretation in this region and simply state that this domain is likely to contribute to the inter-molecular interactions that stabilize the pore, as they have done in the Discussion section, paragraph three.