Since an interaction between hyperglycemia and TNF-α on the induction of NETosis was observed in vitro (Figure 2D), we examined whether this phenomenon was also evident in vivo by analyzing samples taken during the OGTT. This test is routinely used to detect GDM in pregnant women, due to their altered response to a HG challenge, as is evident in our study cohort (Figure S2A in Supplementary Material)(50).