Conventional PCR: 0.8 µM of each primer M-forward: 5′ ACGAAAGTCTGATGGAGCAATA 3′ and M-reverse: 5′ ACGCCCAATAAATCCGRATAAT 3′ . PCR was performed using the Thermo Hybaid PCR express machine using the following conditions, Segment 1: 94 °C 1 min, Segment 2: 94 °C 1 min, 60 °C 1 min, 72 °C 30 min at 45 cycles and Segment 3: 72 °C for 10 min.