Previously, we found that the deletion of either the ARL1 or YPT6 gene causes sensitivity to the drug rapamycin only at 37°, and not at 30° (the atg1Δ strain, the negative control for autophagy, showed growth defects on rapamycin at both 30 and 37°, while the WT strain was able to grow at both temperatures). We further confirmed that this high temperature growth defect on rapamycin was due to a failure to process autophagy normally (Yang and Rosenwald 2016). To determine what genes might suppress the autophagy defect in these strains, we utilized the rapamycin sensitivity phenotype at 37° as a preliminary way to isolate genomic fragments that contain potential high copy number suppressors. Each deletion strain was transformed with the library and 20,000 colonies each were screened for their ability to grow on rapamycin medium at 37°. As a result, 27 distinct genomic fragments were identified from the screen on the arl1Δ strain (Table S2), and 10 were identified for the ypt6Δ strain (Table S3).