Mlc1-tTA transgenic mice were from the RIKEN Bioresource Center (stock no. RBRC05450). Transgenic mice TRE-ErbB2V664E (stock no. 010577) and TRE-dnEGFR (stock no. 010575) were from the Jackson Laboratory. Mlc1-tTA;TRE-ErbB2V664E (Mlc1-ErbB2V664E) and Mlc1-tTA;TRE-dnEGFR (Mlc1-dnEGFR) were obtained by breeding Mlc1-tTA mice with TRE-ErbB2V664E or TRE-dnEFGR mice, respectively. Primers Mlc1U-657 (5′- AAATTCAGGAAGCTGTGTGCCTGC-3′) and mtTA24L (5′- CGGAGTTGATCACCTTGGACTTGT-3′) with a 680- bp PCR product were used for genotyping of Mlc1-tTA, whereas primers 9707 (5′- AGCAGAGCTCGTTTAGTG-3′) and 9708 (5′- GGAGGCGGCGACATTGTC-3′) with a 625- bp PCR product for that of TRE-ErbB2V664E, and primers 9013 (5′- TGCCTTGGCAGACTTTCTTT-3′) and 7554 (5′- ATCCACGCTGTTTTGACCTC-3′) with a 318-bp PCR products for that of TRE-dnEGFR. Unless indicated, mice were housed in a room with a 12-h light/dark cycle with access to food and water ad libitum. Mlc1-dnEGFR, Mlc1-tTA and Mlc1-ErbB2V664E mice with either sex and their littermate control mice with matched sex were used for experiments. Animal experiments were approved by the Institutional Animal Care and Use Committee of the Hangzhou Normal University.