We could only test 47 birds from the 75 individuals (63%) from southern Spain with pox-like lesions, including 33 swabs (13 from 2013 and 20 from 2014) and 14 tissue samples (all from 2014) (Table 1). APV infections were confirmed by molecular amplification in 64% of them, including 26 swabs (79%) and 4 tissues (29%). In central Spain, pox-like skin lesions were observed in ten house sparrows; swab samples were collected from all ten birds and additional tissue samples were collected from five of the ten birds. Overall, APV infection was confirmed in six of the ten house sparrows with avian pox-like skin lesions observed in central Spain. Four of the ten swabs obtained in central Spain were PCR positive, while APV infections were confirmed in each of the five tissue samples tested.