A negative Poisson’s ratio was moderately formed for the PLGA scaffolds. As previously mentioned, there were various factors to form a negative Poisson’s ratio for foam structure such as tri-axial compression, heat treatment temperatures and time. In this study, negative Poisson’s ratio were successfully embodied, and auxetic PLGA scaffold could be fabricated with the permanent volumetric compression ratio of 2.4 and 60 °C/10 min conditions. The MG-63 osteoblast-like cells were entirely attached after 24 h on the scaffold. The bone cells were grown well via the compressive stimulation. From 1-day to 3-day culture, the result showed the effectiveness of negative Poisson’s ratio of the scaffold for bone cell proliferation. It is considered that the auxetic PLGA scaffolds would affect initial period for bone cell proliferation effectively. Hence, we conclude that mechanical stimulation for bone cell cultivation through auxetic scaffolds should have had beneficial aspects and might be play an important role in bone tissue regeneration.