In present study, we investigated the effect of AHG on anti-tumor activity of nature killer (NK) cells and cytotoxic T lymphocytes (CTLs) in vitro. Furthermore, the immunomodulatory effects of AHG on spleen lymphocyte proliferation, cytokines secretion, intracellular free Ca2+ concentration (second messengers to activate lymphocytes), delayed-type hypersensitivity (DTH) reaction, as well as antioxidant activity in cyclophosphamide (CY)-induced immunosuppressed mice were also systematically elucidated in vivo.