Some peculiar varieties have been characterized at a higher number of microsatellites (Bergamini et al. 2013) to ascertain parental relationships. Overall 107 varieties were evaluated at 54 nuclear microsatellites. Additionally, five plastid microsatellites, commonly used for chloroplast haplotype assaying, were screened. They are used to assign the sex of the two parents in a cross, considering the maternal inheritance of plastid genomes in the Vitis genus (Arroyo-García et al. 2006).