Assemble TALENs using a Golden Gate TALEN and TAL Effector Kit 2.0 (Addgene kit # 1000000024) (Cermak et al., 2011). Clone the assembled TALENs into pCS2TAL3DDD and pCS2TAL3RRR vectors (provided by Dr. David Grunwald, University of Utah) (Dahlem et al., 2012), and synthesize mRNAs using the mMESSAGE mMACHINE SP6 Transcription Kit.