The larval zebrafish intestine is not only an excellent model for the study of lipid droplet and lipoprotein packaging, but also a site of differential channeling of dietary fatty acids depending on their chemical properties. The amenability of this model to biochemistry due to the ease of obtaining large numbers of embryos and larvae and performing lipid extractions from them, combined with the transparency of the larva, provides an opportunity unique among vertebrates to perform live imaging and metabolic labeling experiments in parallel using the same fluorescent lipid reagents (28, 77–79). Additionally, the whole-body lipid composition of the larval zebrafish is highly sensitive to changes in diet: the TG content of the 6 dpf larva increases 10-fold 24 h after a single high-fat meal (compared with a standard low-fat diet, and allowing time for the intestinal lumen to clear) (29). (In these experiments, the high-fat meal was chicken egg yolk; ~50% lipid dry weight, and the low-fat meal was SERA Micron larval growth food; 7% lipid. The lipid content of “standard chow” for zebrafish larvae is typically 5–15%.) Working at developmental stages before adipose tissue appears (~14 dpf) avoids signal to noise problems that may occur when the neutral lipid stored in adipose is included in the whole-body lipid profile. Also, at these early developmental stages examination of dietary lipid processing in the intestine can be isolated from potential regulatory influences from adipose tissue.