Then, we evaluated the fractions of each multifunctional cell population expressing all three, any combination of two or single production of cytokines (Fig. 2e and j). Among T CD4+ lymphocytes, the least prevalent populations with two functions were IFNγ+TNF+ and IL2+TNF+ in DENV/ZIKV‐patients compared to exposed donors. Higher frequency of IFNγ+TNF+ CD4+ T cells was observed in DENV‐ compared to DENV/ZIKV‐patients. Finally, we observed increased frequency of single IFNγ+CD4+ T cell population in ZIKV‐patients compared to exposed donors (Fig. 2e).