Four different G. elata tissues (protocorm, juvenile tuber, immature tuber, mature tuber) were collected and total genomic DNAs were extracted using hexadecyl trimethyl ammonium bromide (CTAB). The 16S V4 and ITS1 genes in all sample were amplified using the universal primers 515F-806R and ITS5-1737F with a barcode as a marker for distinguishing samples. The PCR was performed with Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA, UK). PCR products were mixed in equidensity ratios. Then, the mixed PCR products were purified with Qiagen Gel Extraction Kit (Qiagen, Germany). Sequencing libraries were generated using a TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA) following the manufacturer’s recommendations and index codes were added. The library quality was assessed on a Qubit@ 2.0 Fluorometer (Thermo Scientific) and Agilent Bioanalyzer 2100 system. The library was sequenced on an Illumina HiSeq 2500 platform and 250 bp paired-end reads were generated.