A high proportion of cells expressing FOXP3 protein corresponded to a high proportion of cells with a demethylated TSDR in sorted populations (Figures 1B and C., respectively). Similar to TSDR demethylation results, the proportion of cells expressing Foxp3 tended to be higher in CD25hi mTreg (median, 93.6%) compared with CD25int mTreg (median, 83.7%), naive Treg (median, 78.7%), and total Treg (median, 85.2%) (Figure 1C).