From gene network, we randomly selected simulated genes involved with a number of NSCLC-associated genes and then calculated the shortest path within genes (a total of 500 simulations); after we counted once, the Betweenness value in stimulation was bigger than that of actual one. After 500 randomized trials, each gene would get a frequency that was defined as permutation FDR of shortest-path gene of which small value (FDR < 0.05) meant a significantly NSCLC-involved gene of the shortest path.