CAV is a relatively small virus approximately 23 nm in diameter. A total of three open reading frames (ORFs) were involved in the viral genome and have a length of 2.3 kb . These ORFs respectively encode a 51 kDa VP1 protein, a 28 kDa VP2 protein and a 13 kDa VP3 protein. VP2 has dual-specificity phosphatase activity. VP3 is also referred to as apoptin with apoptosis-inducing activity. VP1 is the sole structural protein, which is the major component responsible for capsid assembly [5, 7, 8]. Currently, VP2 and VP3 proteins have been the focus of investigations of virus pathogenicity [9, 10]. In addition to its importance in the viral life cycle, VP3 has also demonstrated apoptosis-inducing activity as well as medical applications for anti-cancer treatments for humans in many previous studies [11–14]. VP1 can interact with VP2 and then significantly elicit the production of virus-neutralizing antibodies in the host in terms of immunogenicity studies . Therefore, VP1 is thought to be a good candidate for an immunogen to develop a subunit vaccine .