Initially, 10 out of 20 patients with IPMN were variant-positive; however, after CV78 filtering, only one patient was variant-positive (the bottom section of Table 1). In contrast, 32 out of 86 patients with pancreatic cancer were variant-positive after CV78 filtering. The CV78 filter decreased the assay sensitivity from 72.1% to 37.2%; the relative reduction in assay sensitivity was similar to that of the first data set. Use of the filter increased the identification of variants present in metastatic cancer, which would have otherwise remained obscure. The major characteristics of the first sample set were reproduced by the second sample set.