Four-micrometer-thick sections of paraffin-embedded kidneys were dewaxed, heated in citric acid solution and next incubated with antibodies. After blockade of endogenous peroxidase, sections were immunostained with a rat anti-F4/80 monoclonal antibody (MCA497GA, ABD Serotec, 1/2000). The mean cell count was performed on 10 sections at 400x magnification. Four-micrometer-thick cryostat sections were fixed with acetone for 7 min. After blockade of endogenous peroxidase, sections were stained with a mouse anti-CD3 monoclonal antibody (A0452, Dako, 1/200). The mean cell count was performed on 10 sections at 400x magnification. Immunostaining was revealed by specific Histofin (Nichirei Biosciences) and AEC (k34769, Dako) and counterstaining was performed with hematoxylin QS (Vector). An aqueous mounting media was used (Scytek laboratory).