We investigated the intra- and inter-individual variability of granulocyte and lymphocyte distributions after sorting. The intra-individual variability for lymphocytes and granulocytes were established through four independent experiments on blood from a single donor, while the inter-individual variability was assessed using experiments on blood from three different donors. Both intra- and inter-individual distributions for lymphocytes were remarkably consistent, with ~60% retention in O6 (Fig. 6A–B). In contrast, granulocyte distribution varied significantly, both intra- and inter-individually (Fig. 6C–D). This result is not surprising as granulocytes are dominated by neutrophils, which represent more than 90% of these cells, and neutrophils are known to adopt different biophysical characteristics upon activation by chemical40 or mechanical stresses41.Figure 6Outlet distributions of lymphocytes and granulocytes. (A,B) Outlet distributions of lymphocytes from three donors. (C,D) Outlet distributions of granulocytes from three donors. Each inter-individual distribution profile data point is a mean of triplicate experiments. The error bars in Fig. 6B and D represent the standard deviation of results from 3 trials.