Twenty-two DEG were selected for further examination by quantitative real-time PCR using the Fluidigm technique (Table 2). These genes were selected from the three studied lists ((List1)), (List 2), and (List 3)). Pearson correlations between the differences in expression measured by quantitative real-time PCR and microarray were greater than 0.70 for 7 genes (CHI3L1, MYLIP, LCK, SOD2, VAT1, COMT, and FAS). Lower correlations (between 0.5 and 0.6) were obtained for GALK2, VNN2, JAK2, KAT5, ADAM10, RARA, and ANXA7. Table 2Correlations between quantitative real-time PCR with microarray expression for selected genes (n=22)Gene nameGene descriptionList that provided the genePearson correlation p-value (Fluidigm)Gene expression profile CHI3L1 chitinase 3 like 1(List 1)0.721.68e-07down at t=+1up at t=+24 CHIT1 chitinase 1(List 1)0.680.00214down at t=+1up at t=+24 CLEC2D C-type lectin domain family 2 member D(List 2), (List 3)0.600.0019down at t=+1up at t=+24 GALK2 galactokinase 2(List 1)0.509.52e-11down at t=+1up at t=+24 HSD17B11 hydroxysteroid (17-beta)(List 1)0.673.02e-11down at t=+1dehydrogenase 11up at t=+24 KAT5 lysine acetyltransferase 5(List 1)0.535.37e-09down at t=+1up at t=+24 LCK LCK proto-oncogene, Src family(List 1)0.743.77e-10down at t=+1tyrosine kinaseup at t=+24 MSN moesin(List 1)0.615.68e-05down at t=+1up at t=+24 MYLIP myosin regulatory light chain(List 1)0.722.26e-10down at t=+1interacting proteinup at t=+24 RAB31 RAB31, member RAS oncogene family(List 1)0.650.00152down at t=+1up at t=+24 RARA retinoic acid receptor alpha(List 1)0.586.59e-05down at t=+1up at t=+24 SSH1 slingshot protein phosphatase 1(List 1), (List 2)0.671.28e-11down at t=+1(List 3)up at t=+24 VAT1 vesicle amine transport 1(List 1)0.751.26e-12down at t=+1up at t=+24 VNN2 vanin 2(List 1)0.520.74down at t=+1up at t=+24 CERS4 ceramide synthase 4(List 2), (List 3)0.601.16e-06down at t=+1up at t=+4 FAS Fas cell surface death receptor(List 1), (List 2)0.83<2e-16down at t=+1(List 3)up at t=+4 JAK2 Janus kinase 2(List 2), (List 3)0.522.54e-10down at t=+1up at t=+4 ADAM10 ADAM metallopeptidase domain 10(List 1)0.568.15e-05down at t=+4up at t=+24 ANXA7 annexin A7(List 1)0.590.000417down at t=+4up at t=+24 COMT catechol-O-methyltransferase(List 1)0.751.64e-14down at t=+4up at t=+24 STMN1 stathmin 1(List 1)0.693.24e-09down at t=+4up at t=+24 SOD2 superoxide dismutase 2,(List 1)0.74<2e-16up at t=+4mitochondrialdown at t=+24 p-value (Fluidigm) gives the time effect of quantitative real-time PCR data for every gene