In this work, to avoid direct UVB exposure and for observing the influence from fibroblasts, culture conditioned mediums from fibroblasts were harvested and used to treat melanocytes. Using the conditioned medium from regular fibroblasts and fibroblasts with UVB treatment, ERK-1, ERK-2, Myo5a, and c-kit genes were upregulated while MITF and Pmel17 were downregulated when treated with UVB-exposed FB medium. According to the above results, UVB-induced SCF, HGF, NRG, and CRH increased. ERK-1 and ERK-2 were significantly increased, attributable to both the SCF and HGF signaling transduction pathways. UVB induced SCF production, so the receptor c-kit expressed on melanocytes increased. In contrast, MITF gene expression was downregulated under UVB-exposed fibroblast medium treatment. McGill et al. indicated MET, the HGF receptor, was regulated by MITF, since MET was a transcriptional target of MITF. In this case, HGF was increased from the conditioned medium and caused high MET expression in the melanocytes, so MITF decreased to reduce MET overexpression .