A mammalian recombinant DENV Non‐Structural‐1 (DENV NS1) from all four serotypes was used as stimuli for ELISPOT. This protein was donated by The Native Antigen Company (https://thenativeantigencompany.com/product/dengue-virus-ns1-protein-serotypes-1-4/?doing_wp_cron=1480736436.0465950965881347656250). Phytohemagglutinin (PHA), phorbol 12‐myristate 13‐acetate (PMA), ionomycin, Brefeldin A, and Saponin were supplied by Sigma–Aldrich (St. Louis, MO, USA). Detailed information of all mAbs used in this study is listed in Table S1.