A total of 108 samples were collected; 44 samples were raw milk (28) Cow, (9) she-camel and (7) goat, and the remaining 64 samples were locally manufactured dairy products: Soft cheeses (10 Ricotta and 21 Massora: Locally made cheese that manufactured by simple method of casein precipitation using food grade diluted organic acids), (5) ice cream and (28) fermented cow’s milk. Samples were randomly collected from different regions of Libya (Janzour, Tripoli, Kremiya, Tajoura, and Tobruk). Collected samples were transferred to the laboratory of Food Hygiene and Control Department, Faculty of Veterinary Medicine, University of Tripoli in an insulated icebox with a minimum of delay to be immediately examined for isolation and identification of existing EHEC.