Transfection ratio of the Lenti-virus-containing Lenti-GSN vector and GSN overexpression in YTS cells. (A) Green fluorescence was observed in the transfected YTS cells under a fluorescence microscope (×200 magnification) at 48 h, which indicated a successful transfection. (B) Flow cytometric analysis demonstrated that the transfection ratio in cells was 70-80% at 48 h. (C) qRT-PCR analysis exhibited that GSN mRNA expression was higher in YTS-GSN cells than in YTS-Con cells at 48 h. (D) Western blot analysis showed that the level of GSN protein was higher in YTS-GSN cells than in YTS-Con cells at 48 h. GSN, gelsolin; YTS cells, nontransfected cells; YTS-Con cells, YTS cells transfected with the pCDH-CMV-MCS-EF1-copGFP vector; YTS-GSN cells, YTS cells transfected with the pCDH-CMV-MCS-EF1-copGFP-GSN vector. **P<0.01, ***P<0.001.