The air-dried leaves were ground in a mill, and then passed through a mesh screen to obtain a uniform 40 mesh size. The powder was extracted with methanol for 12 h, and then treated by ultrasonic waves for 30 min at ambient temperature. The supernatant was evaporated to dryness under reduced pressure using a rotary evaporator. The crude methanol extract was dissolved in a small amount of methanol and extracted with ethyl acetate. The ethyl acetate extract was purified using XAD-2 macroreticular resin and eluted with MeOH: H2O: CHC12 (85: 10: 5). The elution liquor was concentrated and subjected to column chromatography over silica gel (200–300 mesh) which was first eluted with dichloromethane to remove non-polar compounds, followed by a mixture of dichloromethane: ethyl acetate 98: 2. The eluent was collected and found to be effective against P. myriotylum. After freeze-drying, a pale-yellow oily product was stored in an airtight sealed glass vial at 4°C for further testing. The yield of the oil extracts was 0.92%.