The flanking sequences for these four SNPs were used in Blast analysis. The best BLAST (BLASTn) analyses were performed against the NCBI database (National Center for Biotechnology Information) for non-redundant protein database. The best hit resulted in the identification of the single gene (Mt) from a single contig (contig 03321), within which fell all four identified SNPs (see Additional file 8B). The gene (Mt) encodes an mRNA for metallothionein-like protein (metal ion binding) (Table 6).