CCK-8 assay was performed to detect the growth of YTS, Lenti-Con-transfected YTS, as well as Lenti-GSN-transfected YTS cells. Cells were seeded in 96-well plates at a density of 1×104 cells/well and incubated for 24, 48, 72 or 96 h in a humidified incubator. Subsequently, 10 μl CCK-8 solution (7Sea PharmTech, Shanghai, China) was added to the wells at the indicated times. After incubation for 3-4 h, absorbance was detected using the using a multilabel counter (Enspire Multimode Plate Reader, PerkinElmer) at 450 nm.