Linezolid (Zyvoxid®, Pfizer, United States) was delivered (125 mg of LNZ in 10 mL of 5% α-cyclodextrin solution) over 1 day through two subcutaneous injections (50 mg/kg), 4 and 16 hours after bacterial challenge, as previously described . Blood samples were obtained 1, 2, 4, 6 and 12 hours after the first drug injection and stored at -80°C. Linezolid concentrations were then determined by high-performance liquid chromatography (limit of detection = 0.03 mg/L).