A licensed pathologist who was blinded to tissue genotypes performed quantification of tissue areas. Four images were quantified per section representing 1) the area of greatest neoplastic progression, 2) the area of lowest neoplastic progression, and 3,4) areas of the pancreas that were consistent with the average progression for that tissue section. Acini, ducts, PanINs, ADM lesions, and stromal tissue not including blood vessels were manually outlined using ImageJ software and are graphed as the percentage area of all areas quantified.