Right kidneys were used for preparation of total RNA using Trizol (Thermo Fisher Sci, Inc., Waltham, MA). qRT-PCR (Omniscript, QuantiTect, Qiagen, Valencia, CA) was performed in an ABI-Step One Plus Cycler (Fisher, Life Technologies, Waltham, MA) using the mouse beta actin forward primer: GGCTGTATTCCCCTCCATCG, and reverse primer: CCAGTTGGTAACAATGCCATGT, the mouse Enpp1 forward primer: CTGGTTTTGTCAGTATGTGTGCT and reverse primer: CTCACCGCACCTGAATTTGTT, the mouse Entpd5 forward primer: CCAAAGACTCGATCCCCAGAA and reverse primer: TGTTAGAAAGTTCACGGTAACCC, the mouse Ank forward primer: TACGGGCTGGCGTATTCTTTG and reverse primer: CACTGTAGGCTATCAGGGTGT, and the mouse Tnsalp forward primer CCAACTCTTTTGTGCCAGAGA and reverse primer: GGCTACATTGGTGTTGAGCTTTT.