We analyzed mRNA expression profiles from TCGA breast cancer cohort (n=500). miR-20a expression correlates negatively with that of BECN1, ATG16L1 or SQSTM1 in breast cancer (Figure 7a). The mRNA expression patterns of BECN1, ATG16L1 or SQSTM1 were also compared across different breast cancer subtypes. In TCGA data set, the gene expression of BECN1, ATG16L1 or SQSTM1 were markedly lower in triple-negative breast cancers (n=82) compared with other subtypes (n=391) (Figure 7b).