Additionally, for a subset of 94 DNA samples of progeny and parents of the ‘Horizon’ × V. cinerea B9 family, the following SSR markers located near the resistance loci were genotyped: VVIB22 (Merdinoglu et al. 2005), VrZAG62 (Sefc et al. 1999), VVMD7 (Bowers et al. 1996) and SC8_0040_088 (Jaillon et al. 2007). PCR reactions were performed with 6 µl of QIAGEN Multiplex PCR Plus Kit (Qiagen, Germany), 1 µl of primer mix (0.5 µM each) and 5 µl of each DNA sample diluted 1:10. PCR amplification was performed with 30 cycles of 95 °C for 30 s, 57 °C for 90 s and 72 °C for 90 s, followed by 68 °C for 30 min. Fragment sizes were determined relative to a LIZ 500 Size Standard using an ABI 3730xl (Applied Biosystems, USA) at the Genomics Facility of the Institute of Biotechnology at Cornell University. Allele calls were generated using GeneMarker V 2.4.0 (SoftGenetics, USA).