We designed human and murine DHX29 sgRNA as previously described . Four hDHX29 and 4 mDHX29 sgRNAs were designed and cloned into the BsmB1 site of lentiCRISPR vector containing Cas9-P2A-puromycin as previously described  and verified by sequencing analysis. The sgRNA-containing plasmids were transfected into 293T cells with pCMV-VSV-G plasmids and pΔ8.9. After two days, the virus-containing medium was subjected to ultracentrifugation (20000 x g at 4°C for 2 h) and frozen at -80°C. Murine Bone marrow-derived dendritic cells (BMDCs) were generated by flushing bone marrow cells from femurs and tibiae of mice and were cultured in RPMI media containing 10% FBS supplemented with GM-CSF and IL-4. Peritoneal macrophages (pMAC) were obtained by injecting mice with 4% (v/v) thioglycollate (Beckton Dickson), and peritoneal cavities were flushed after 3 days with RPMI media/2% FBS. Isolated PBMCs, BMDCs, and pMAC were transduced with 4 mixed DHX29-sgRNA-containing lentiCRISPR viruses, respectively. Transduced cells were selected in the presence of puromycin (Invivogen) for 48 h and subjected to ligand stimulation or viral infection.