To obtain anti-rCsTegu20.6 sera, Sprague–Dawley rats (Orient Bio Inc., Seongnam, Korea) were used for peptide injection. The initial injection included Freund’s complete adjuvant (Sigma-Aldrich, St. Louis, MO, USA) mixed with purified rCsTegu20.6 (100 µg), and 3 subsequent injections included Freund’s incomplete adjuvant (Sigma-Aldrich) at 1-week intervals. Blood was collected 2 weeks after the final immunization, and its serum was separated as the antisera against CsTegu20.6. The specificity of the antibody was detected by Western blot as described below.