Association of CBS hotspot mutations and cis-gene expression. a, d, g Association between mutation status of the CBS hotspot and expression levels of neighboring genes (two-sided Wilcoxon rank-sum test). Upregulated genes are shown above the x-axis, and downregulated genes are shown below the x-axis. Non-expressed genes are shown with empty circles on the x-axis (normalized count < 10 in all samples). b, e, h The reference sequence and mutated alleles at the three CBS hotspots. The mutations in tumors with expression data are underlined. c, f, i The gene expression of CENPQ (c), KCNQ5 (f), and SPG20 (i) in normal gastric tissue, and tumors with and without mutations at the corresponding CBS hotspot. P-values were adjusted using the Benjamini–Hochberg method