Exponential curves were fitted to the mean age at pupation, age at emergence and age at adult death large-scale to temperature. The age at an emergence-temperature function (Additional file 1: Table S3) was adjusted by 14 days to estimate the generation time as we found that eggs were laid two days after an offered artificial blood meal and about 14 days after adult emergence in the laboratory in the studied population (unpublished data). However, oviposition only occurred once, and eggs did not hatch (for possible reasons see [47, 48]).