Behavioral tests have been extensively used to study the visual recognition of animals. Recent developments in virtual reality (VR) tests of animal behavior has provided more consistent testing conditions, less human-animal interaction, opportunities to use a wider variety of experimental parameters, and more precise measurements of the perception of the tested animals. VR tests have been applied across a wide variety of disciplines from disease treatment in humans, to study of navigation and memory in primates, rodents, and even insects [1–9]. Despite the fact that many VR tasks use visual cues, most of the experiments in rodents which use VR probe the function of the hippocampus or memory formation/retrieval but not visual perception [10–17]. Currently, the most commonly used behavior test for visual function is the optokinetic test [18–21]. However, this test does not measure visual perception, but a reflex response. In addition, the optokinetic test is limited to testing spatial frequency or contrast sensitivity to horizontally drifting vertical bars . Many fundamental questions, such as how quickly an experimental animal can learn to recognize certain visual targets, how significantly the complexity of visual cues affects the training requirements and performances of animals, and how long the visual memory will last once animals are trained, remain unaddressed.