Towards the history and advances in the field of experimental detection of clustered DNA lesions, the reader can refer to the above mentioned references. Current research in this field is based on the idea that theory and predictions do not always coincide with experimental evidence. The major challenges towards the detection of clustered DNA damages have been: (1) the accurate measurement of DSBs and OCDLs levels and their types, especially at the cellular level and (2) theirin situ detection and reliable quantitative measurement.