Mitochondrial health using mitochondrial membrane potential (TMRM) and mitochondria‐specific ROS indicator (MitoSOX), nitric oxide levels (Griess reagent), and cell death (using LDH assay) in hSOD1‐WT‐ and hSOD1‐G93A‐expressing NSC‐34 differentiated cells cultured in the presence or absence of P110 (1 μM for 24 h). Results are presented as percent or fold of MOCK (empty vector); n = 3 performed in quintuplet; probability by one‐way ANOVA (with Tukey's post hoc test). Scale bar: 50 μm.Cell death (using LDH assay), mitochondrial‐specific ROS production (MitoSOX), mitochondrial membrane potential (using TMRM), and nitric oxide levels in hSOD1‐WT‐ and hSOD1‐G93A‐expressing NSC‐34 differentiated cells were determined following H2O2 injury in the presence or absence of P110 (1 μM/24 h); n = 3 performed in quintuplet; probability by one‐way ANOVA (with Tukey's post hoc test).Co‐immunoprecipitation of Drp1 with Fis1, Mff, Mid49, or Mid51 was analyzed in hSOD1‐WT‐ and hSOD1‐G93A‐expressing NSC‐34 differentiated cells, cultured in the presence or absence of P110 (1 μM for 24 h). n = 2 performed in duplicates (pooled).Levels of Drp1 were determined in mitochondrial fractions by immunoblotting in hSOD1‐WT‐ and hSOD1‐G93A‐expressing NSC‐34 cells as above, cultured in the presence or absence of P110 (0.25, 0.5, 1 μM for 24 h); VDAC, a mitochondrial membrane protein, was used as a loading control, whereas enolase, a cytosolic marker, was used to assess the mitochondrial sample purity. n = 2 performed in duplicates (pooled).Data information: Mean, standard deviation, and P‐values are shown. Results are presented as percent or fold of MOCK (empty vector). Source data are available online for this figure.