In IHC, 276 (73.2%) and 101 (26.8%) patients were in Lgr5 low and Lgr5 high expression groups, respectively. The results showed that Lgr5 high expression group had remarkably more patients with >60 years (p=0.001), male (p=0.024), macroscopic type III (p=0.030), tumor size 4cm-7cm (p=0.044) and TNM III stage (p=0.046) than Lgr5 low expression group. Multivariate analysis revealed that Lgr5 expression was only independently associated with age (p=0.001, OR=1.756, 95%CI [1.263-2.442]).