This study is the first to identify and validate Hnrnpab and Stx5a, as optimal reference genes for the normalisation of gene expression data during the peak and resolution phases, respectively, of the BMDM response to LPS. Moreover, this study demonstrates the consistency in expression levels of both Hnrnpab and Stx5a for peritoneal and RAW 264.7 macrophages stimulated with LPS. Importantly, the expression levels of these genes are more stable than those of Gapdh and Actinb, which are both commonly regarded as reference genes in the assessment of macrophage inflammatory responses. These observations demonstrate the importance of assessing the stability of reference genes for every experimental condition prior to normalisation of gene expression.