RUNX1 and ETV6-RUNX1 proteins were detected via immunoblot using an anti-RUNX1 mouse antibody (#110035, clone 5A1, Abcam) diluted at 1:300. ETV6 proteins were detected via immunoblot using an anti-ETV6 mouse antibody (#54705, Abcam) diluted at 1:1000. As a loading control, HSC70 protein levels were assessed using a mouse-derived antibody (#7298, clone B-6, Santa Cruz) diluted at 1:500. The immunoblots were visualized with enhanced chemiluminescence Western blotting detection system (WBKLS0500, Merck Millipore) according to the manufacturer’s instructions.