OEC‐CM increased SH‐SY5Y cell viability and reduced H/R injury mediated damage. (A) MTT‐viability tests performed at 0, 3, 8 and 24 hrs. Values of spectrophotometric determination at 550 nm are reported as percentage of cultures at time 0, considered as 100%. Data are shown via standard box‐and‐whiskers plots in which the central‐line represents the median, the upper‐ and lower bounds of the boxes are min and max value and dots represent all value expressed as percentage of control. NS not significant, *P < 0.05, **P < 0.01 and ***P < 0.001 versus CTRL Normoxia time 0. ####P < 0.0001 (manovas and Tukey honest significant difference (HSD) post hoc test). (B) Western blot analysis of Cleaved Caspase‐3 levels on SH‐SY5Y in control and H/R injured cultures. Proteins levels are plotted as fold change over 3 hrs after treatment in control culture considered as 1. **P < 0.01, ***P < 0.001 (manovas and Tukey honest significant difference (HSD) post hoc test). (C) Western blot analysis of Cleaved Caspase‐3 levels on SH‐SY5Y in OEC‐CM conditioned cultures in normoxic and in H/R injured cultures. Proteins levels are plotted as fold change over 3 hrs after treatment in OEC‐CM normoxic cultures considered as 1.