To determine whether miR-5096 effects are stable with time, its cell expression level was determined two days after cell loading. More precisely, donor U87 were plated 2 days after transfection with acceptor HMEC for 12 h (ratio 1:1). As shown in Figure 5A, miR-5096 expression level in U87 was lower after 2 days in culture than within the first day (by 30 %; see Figure 2C). Its expression level in HMEC was also decreased (by 70% of its value measured within the first day; see Figure 2D). Thus, after 2 days, U87 were still capable to transfer miR-5096 to co-cultured HMEC but to a lesser extent.