Zeta potential measurement was further performed to identify the supramolecular assembly surface charged distribution (see Supplementary Figs S20 and S21), both of the 2C-WP5A+trans/cis G assembly giving an average negative zeta potential to maintain the stability of the assembly. Based on the above experimental results and analysis, we postulate that the formation of the supramolecular assembly occurred in two steps. Firstly, the 2C-WP5A and G instantaneously formed a host-guest complex. Subsequently, excess guest molecules arranged around the host-guest complex due to electrostatic effect with redundant carboxylate anion of 2C-WP5A. According to the experimental results of the zeta potential, the 2C-WP5A was on the surfaces of the nanoparticles. Based on the above description, the poly-anionic structure of 2C-WP5A is very important in the formation of the supramolecular nanoparticles assembly.