In addition, we demonstrated that liraglutide acutely decreased the gene expression of hypothalamic 5-HT2A receptors in mice, which are responsive to the acute anorexic effects of liraglutide. Despite the chemical inhibition of 5-HT synthesis, the gene expression of hypothalamic 5-HT2A receptors was not changed. Thus, changes in brain 5-HT synthesis and the gene expression of hypothalamic 5-HT2A receptors could be independent, and the decrease in hypothalamic 5-HT2A receptor gene expression induced by liraglutide is unlikely to be compensatory to an increased brain 5-HT levels, although protein levels of the receptor were not measured here and could represent another regulatory mechanism.