Silencing ODC expression by shRNA suppresses anchorage-dependent and anchorage-independent ESCC cell growth. a ODC expression was analyzed by Western blot in KYSE450 and KYSE510 cells expressing shMock or shODC. b ODC activity was assessed as the release of L-[1-C14] ornithine and results are shown as percentage of the control group (set at 100%). c Anchorage-dependent cell growth was measured by MTS assay. d For anchorage-independent growth, cells were cultured in soft agar for 3 weeks and then colonies were counted using a microscope and the Image-Pro Plus software (v.6.0) program. All data are shown as means ± S.D. of triplicate values from 3 independent experiments