The neutralization reactivity with V1V2 region mutants of the 2B10 and 2E4 scFvs were performed using HIV-1 subtype C pseudovirus 16055 and its V1V2 mutants N156K, N160K, K169E, and K171A using a starting concentration of 100 µg/ml of scFvs. Neither of the two scFv monoclonals shows V1V2-dependent neutralization activity.