In qPCR, 43 (46.2%) and 50 (53.8%) patients were divided into Sox2 low expression and high expression groups. Sox2 expression was notably associated with age ≤60 years (p=0.008) and T3-4 stage (p=0.030) in univariate analysis. In logistic regression, only age was the independent related factor to Sox2 (p=0.009, OR=0.322, 95%CI [0.137-0.755]).