KL designed and planned the study, edited and finalized the manuscript. SS provided scientific inputs in phage library construction and in discussion section of the manuscript. RL and SKK provided the HIV-1 infected pediatric donors sample. SK performed the research, data analyses, and wrote the manuscript. RK helped SK in construction of phage library; MAM generated the pseudoviruses for neutralization assays; RT, MM, and MA helped SK for the purification of scFv protein, LK helped SK in competition ELISA and affinity determination of scFv monoclonals.