12) For a methods paper, little information is given on the approach used for designing the left arm and right arm homology sequences and the TALENs. For a general audience, this needs to be expanded. Additionally, the Materials and methods should be expanded to include all details (including relevant product numbers) for vector construction and the specific steps that would be required for using the Zwitch construct to target other genes, such that anyone with reasonable molecular biology skills could follow step-by-step.