Fig. 4: miR-484 and miR-744 expression levels are downregulated in Dengue virus (DENV)-infected Vero cells. The expression pattern of both miRNAs at different times post-infection in Vero cells infected with DENV-1 to 4 was determined by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). The results are expressed as the fold change for each miRNA expression level in each sample relative to the mock-infected samples and normalised to 18S rRNA using the 2-ΔΔCt method; (A-D). Data are shown as median and error from three repeated experiments. Three independent replicates were performed for each experiment. (*) Statistically significant difference compared to the control (p < 0.05).