Dahl SS rats are known to develop hypertension when fed a high-salt diet (Rapp, 1982). First, we confirmed this effect using the DSI telemetry to monitor blood pressure in the rats fed 0.4, 4, or 8% NaCl diets between 8 and 11 weeks of age. The experiment revealed that during the 3-week period the 0.4% diet does not alter blood pressure, whereas the 4% diet raised MAP, and the 8% diet augmented the development of hypertension (Figure 1A). High NaCl diets increased sodium excretion proportionally to sodium content in the chow whereas potassium excretion remained stable, suggesting that food intake was similar on all diets (Figures 1B,C). While 4% diet represents a widely used dietary challenge, 8% diet had more profound and faster effects on blood pressure. Therefore, we used 8% in our subsequent experiments. At the end of the experiments, kidney samples were collected for the assessment of protein expression. No significant differences in terminal total body weights were found: average mass was 325.7 ± 9.7 (0.4% NaCl) vs. 308.5 ± 1.3 g (8% NaCl).