Our initial objective was to compare NS1 DENV‐specific memory response from dengue‐exposed donors with dengue‐infected individuals. Our data indicate that DENV NS1 memory response had the highest frequencies and magnitude in healthy dengue‐exposed donors compared to those with active DENV infection. Among DENV ingle‐infected patients, the 22‐days DENV patient 2 showed the best response to NS1, indicating that latter phases of dengue could be a better time point for evaluating specific cell responses through ELISPOT. Our second goal was to determine whether active ZIKV infection could affect the NS1 DENV‐specific memory response in two different situations: in DENV‐exposed patients or in those who have current ZIKV or DENV/ZIKV infections. Our data showed that DENV NS1 was not able to induce a response in any acute active ZIKV‐patients, even the samples being positive for Dengue IgG ELISA test. Two out five DENV/ZIKV‐patients responded with above 50 IFNγ SFC following DENV NS1 stimulation, while the other three responded with below 20 IFNγ SFC after stimulation (Fig. 3b). Finally, the magnitude of IFNγ SFC response was not statistically different among the groups of acute patients. In order to understand the specificity of this response, we intend to evaluate healthy donors exposed to Zika in the future.