Following fixation in 4% paraformaldehyde (PFA), the specimens were paraffin embedded. They were then subjected to dehyrdration and rehydration cycles. Specimens were subsequently stained in Hematoxylin for 10 minutes. They were then briefly washed in running tap water for 3 seconds after which they were differentiated in acid alcohol for 6 seconds. This was a 1% concentration solution consisting of hydrochloric acid and 70% ethanol. Specimen were run through tap water until becoming blue in colour. Staining was conducted in 1% eosin for 5 minutes. Slides were analysed with the use of light microscopy (EVOS, XL Core) (n = 3).