The YS602 strain from Saccharomyces cerevisiae (a generous gift from Professor Maurille J. Fournier from UMass Amherst) was used to generate Sbp1-depleted strain (ΔSbp1::kan) by homologus recombination using standard methods, as previously described (Baudin et al. 1993; Wach et al. 1994). Monocistronic luciferase reporters were generated by inserting the firefly luciferase gene between BamHI and KpnI restriction sites of the pUC18 vector (Clontech). A poly(A)62 was introduced between KpnI and EcoRI restriction sites downstream from the luciferase gene. 5′UTRs of Pab1 and eIF4E, including a 36-nt-long endogenous sequence in the open reading frame to maintain the AUG context, were cloned into XbaI and BamHI restriction sites of pUC18, in-frame and upstream of the luciferase gene. RNAs with sequence complementary to the 5′UTRPab1 and 5′UTReIF4E, termed 5′UTRPab1-complement and 5′UTReIF4E-complement, were used as an RNA length and sequence control. The 5′UTRPab1-complement and 5′UTReIF4E-complement were generated in the same way as the 5′UTRPab1. The 5′UTRCrPV sequence was amplified from a pEJ1014 vector (a generous gift from Professor Eric Jan from the University of British Columbia) (Ren et al. 2012) and also cloned in the same way as the 5′UTRPab1.