High-fat diet feeding induced remarkable obesity in mice. (A) Body weight, (B) fasting blood glucose level, and (C) glucose tolerance assayed by an intraperitoneal glucose tolerance test (IPGTT) before and after 6 months' high-fat diet feeding. All values are means ± SE (n = 11 for each group). Differences of (A) body weight, (B) fasting blood glucose between before and after HFD feeding were analyzed using paired Student's t-test method. Differences of (C) blood glucose levels at indicated time point during IPGTT between before and after HFD feeding is analyzed using two-way RM ANOVA method. **p < 0.01; ***p < 0.001.