Chemokines and their receptors are key drivers of inflammation. Our goal here was to assess the magnitude of T cell activation by ex vivo expression of chemokine receptors, particularly useful for dissecting T‐cell subsets with distinct migratory capacity and effector function. As shown in Figure 1, expression of ex vivo chemokine receptors such as CCR5, CX3CR1, and CXCR3 were consistently detected on CD4+ and CD8+ T cells from all acute patients and healthy exposed donors. DENV/ZIKV infected individuals presented higher frequencies of CCR5+ or CX3CR1+ compared to exposed donors. Although not significant (p = 0.0571), we observed that DENV or ZIKV infected individuals have a propensity to have high CX3CR1+ frequencies compared to exposed individuals. (Fig. 1b,c). No appreciable differences were detected in CXCR3 expression on CD4+ T cells among all groups (Fig. 1d). Intriguingly, among patients analyzed in DENV group, the lowest values for all chemokine receptors expression on CD4+ T cells were observed for patient 2, in which cells were obtained 22 days post infection, even though viral genome was still detected.