The Guarapuava and Porto Barreiro populations presented low genetic distance (Table 5), high gene flow (Table 6), they occupied the same area in the PCoA quadrants (Fig 3), and formed a single genetic group in the Bayesian analysis (Fig 4). This behavior leads to the hypothesis that these populations may assume a metapopulation model. The Marialva, Guarapuava, Porto Barreiro, Xanxerê, and Campos Novos populations were more similar in all analyzed variables, suggesting that they are more related than when compared to the Coxilha and Panambi populations. This finding is even more evident in the dendrogram (Fig 5). The populations of Xanxerê and Campos Novos are genetically closer and physically distant to Marialva population, while genetically distant and physically closer to Coxilha and Panambi. This observation indicates a possible geographic barrier to gene flow between the populations of Xanxerê/Campos Novos and Coxilha/Panambi.