Levels of Drp1 were determined in mitochondrial fractions by immunoblotting in hSOD1‐WT‐ and hSOD1‐G93A‐expressing NSC‐34 cells as above, cultured in the presence or absence of P110 (0.25, 0.5, 1 μM for 24 h); VDAC, a mitochondrial membrane protein, was used as a loading control, whereas enolase, a cytosolic marker, was used to assess the mitochondrial sample purity. n = 2 performed in duplicates (pooled).