Calcium ions, known as the most widely used intracellular secondary messengers, play an essential role in lymphocyte function, which participate in proliferation, differentiation and gene transcription of lymphocyte . The elevation in the concentration of Ca2+ in the cytosol triggers the activation and proliferation of lymphocytes, especially promotes the transcription factors translocating from cytoplasm to the nucleus and binds to the promoter. The final consequence is initiating the transcription of specific cytokine genes . It has been mentioned that PSG-1, the polysaccharide obtained from Ganoderma atrum, may activate spleen lymphocytes via Ca2+/CaN/NFAT/IL-2 signaling pathway, similar to the polysaccharide fraction of Panax ginseng . In our study, the concentrations of Ca2+ were noticeably increased after AHG administration. Particularly, Ca2+ concentration in the AHG group at 100 mg/kg reached the level of that in NC group. These results showed that AHG was capable of recovering the decrease of the concentration of Ca2+ in CY-treated mice to the normal level, which may be the root of the enhancement of splenocyte cytokines secretion.