We did not detect any CMt in the cats sampled in this study. Other prevalence studies have previously shown that CMt seems to be the least prevalent haemoplasma in Europe, ranging from 0 to 2.3% [12, 21, 26, 27]. In these studies, the sample sizes were between 3 to 20 times larger than our number of cats. A larger sample size could therefore be needed in order to conclude whether CMt is present in Denmark.