(A) Live cell imaging of LX2 cells transfected with ER-reporter RFP-KDEL plasmid and subsequently stained with NRB-AF12. (B) Immunostaining of LX2 cells for the endoplasmic reticulum marker calreticulin; cells were counter-labeled with NRB-AF12. (C) Confocal live cell imaging of LX2 cells fluorescently labeled with 500 ηM NRB-AF12 and 500 ηM ER-Tr red. Insets show magnification of the pictures. Magnification 60x; scale bar 10 μm. (D) Quantification of NRB-AF12 co-localization with RFP-KDEL, Calreticulin and ER-Tr is shown as Pearson's coefficient. (n ≥ 10).