Reduction in body weight may be a consequence of lower food intake and/or enhanced energy expenditure. In current study, supaglutide therapy exerted anorectic effects in the obese mice. The energy expenditure of the mice was studied by indirect calorimetry using CLAMS. According to our data, VO2, VCO2, and EE expressed as per-whole-animal showed a trend, but not statistically different, of increase in supaglutide group. However, when normalized by BW, VO2, VCO2, and EE of the supaglutide-treated mice demonstrated significant increase in the dark cycle.