Melanin synthesis is important to fungal development and differentiation19,22; however, the processes potentially genotoxic and cytotoxic due to the generation of semiquinones, quinones, and ROS by the oxidation of polyphenols by tyrosinase (EC 1.14.18.1)23,24; ROS are known as trigger of PCD25. During T. melanosporum development tyrosinase activity steadily increases from the developmental stage 3 to stage 526 so that it is expected an increase of ROS and genotoxic species generation within the FBs, in fact evidences of PCD appear at the developmental stage 5.