We have examined the candidates for TGC-specific AIs from the literature and our experiment. We decided to include Tubb2c, Atp6v1a, Hsc70t, Fbp1, Lrrc34, Gapdhs and Dazap1 as the candidates for TGC-specific AIs from the results of real-time PCR (tissue specificity, age, cell type) in this study. In addition, we selected to include Atp6v1a, Hsc70t, Fbp1 Lrrc34, Gapdhs, and Dazap1 based on molecular weight and age from the literature as potential candidate TGC-specific AIs. Furthermore, we have decided to include Lrrc34, Hsc70t and Dazap1 from testicular localizations, as described in the literature as candidate TGC-specific AIs. Finally, the recombinant proteins of identified 10 (except unnamed protein) TGC-specific AIs were created by using human embryonic kidney 293 (HEK293) cells and these antigencities were reconfirmed by Western blot using EAO serum reaction. The results indicated Atp6v1a, Hsc70t, Fbp1 and Dazap1 were candidates for TGC-specific AIs.