Others have adopted similar workflows to extract ECM proteins in a number of tissues.66,68–70 Of note is the Texas 3-step extraction method by Lindsey’s group.66 In their method, applied to mouse hearts, a similar sequential extraction consisting of NaCl and GuHCl extraction steps as well as the SDS decellularization2,4,57 are performed. In addition, the Texas 3-step method includes further extraction of the insoluble protein pellet after incubation in GuHCl for 48 h. Notably, the vast majority of ECM proteins are identified in the GuHCl fraction. The pellet, however, contains few polymerized proteins, which are not extracted by our ‘English Quickstep’ method (Figure 3C).