The initial aflatoxin B1 (AFB1) concentration in the A. flavus-colonized maize placed in satchels was 13.9 μg of AFB1 per kg of maize (SE = ± 0.65 μg/kg). After three months of storage in Indiana, the AFB1 concentration decreased to 2.07 μg of AFB1 per kg of maize (SE = ± 0.09 μg/kg) and 2.72 μg of AFB1 per kg of maize (SE = ± 0.33 μg/kg) in the PICS and woven bags, respectively. A similar reduction was measured in the Arkansas grain, with 2.28 μg of AFB1 per kg of maize (SE = ± 0.16 μg/kg) in the PICS bags and 2.05 μg of AFB1 per kg of maize (SE = ± 0.12 μg/kg) in the woven bags. For both bag types and both locations, no aflatoxin was detected in the non-inoculated grain placed in satchels adjacent to the A. flavus-colonized maize satchels.