Among the 5648 GO terms appearing once, the top five most highly represented groups in the biological processes category were genes/transcripts associated with signal transduction, response to abscisic acid, cellular response to DNA damage stimulus, methylation and response to water deprivation (Table S6, for enriched cellular components and molecular functions see Tables S7 and S8, respectively). More detailed classification of the biological process GO category also showed enrichment in comparison with whole UniProt for response to cold and oxidative stress, flower development, flavonoid biosynthesis and embryo development ending in seed dormancy as highly represented groups. Overall, this functional characterization and GO enrichment analysis confirmed gene expression for response to water deprivation, cold and oxidative stress, flavonoid biosynthetic process and embryo development ending in seed dormancy reflecting the adaptation of subterranean clover to the harsh Mediterranean environment.