(A) Representative photographs of 42 day-old plants of Col-0, flm-3, and Nd-1 grown at 15°C in long day photoperiod. The plant images were spliced together but originate from the same photograph as indicated by a white vertical line. (B) Quantitative flowering time analysis of Col-0, flm-3, and Nd-1 grown at 15°C and 23°C in long day photoperiod. (C) and (D) qRT-PCR analyses of FLM-β (C) and FLM-δ (D) expression at 15°C and 23°C in ten day-old flm-3 mutants complemented with a gFLMCol-0 or gFLMCol-0+LINE allele (Lutz et al., 2015). Pools of 35–40 independent T2 transgenic lines were used in comparison to Col-0 and Kil-0 homozygous lines. Shown is the mean and SD of three (Col-0, Kil-0) and four replicate pools comprising each 8 to 10 transgenic lines. Percentages refer to the reduction in FLM expression between the gFLMCol-0+LINE transgenic line in comparison to the gFLMCol-0 line or between Kil-0 and Col-0, respectively. Similar letters indicate no significant difference of total leaf number (Tukey HSD, p<0.05).