CalpTG mice had a reduced beta-galactosidase activity, a marker of senescence in response to oxidative stress, in tubular cells at 2 years (Fig. 2H–J, p = 0.031). The expression of p21, another marker of senescence was also reduced in aged CalpTG mice (Fig. 2K, p = 0.01). Kim-1 expression, a marker of tubular injury was significantly increased with aging in WT mice but not in CalpTG mice kidney cortex (Fig. 2L, p = 0.0043).