Pre-thermal stress sampling time points differed in three of the variables analyzed (Table 1). There was a significant effect of sampling time (F1, 1 = 8.45, P = 0.006) and thermal cycle (F1, 1 = 6.70, P = 0.014) with no significant interaction (F1, 1 = 1.23, P = 0.276) in WB glucose. Significant differences occurred in incipient pre-exposure values for liver HSP70, where there was an effect of sampling time (F1, 1 = 27.62, P = <0.001) and thermal cycle (F1, 1 = 8.28, P = 0.008), but not the interaction (F1, 1 = 0.28, P = 0.601). Significant differences were also observed in RBC HSP70 where there was an effect of sampling time (F1, 1 = 16.88, P = < 0.001) and thermal cycle (F1, 1 = 182.09, P = <0.001), but not the interaction (F1, 1 = 0.06, P = 0.11; Table 1). No significant effects of sampling time or thermal cycle were observed in CTMax, WB lactate, liver Ub or RBC Ub (see Table 1). Table 1:Two-way ANOVA performed on physiological variables of juvenile Atlantic salmon exposed to pre-thermal stress sampling conditions (16–21°C)EndpointTimeTreatmentInteractiondfF-statisticP-valuedfF-statisticP-valuedfF-statisticP-valueCTMax**–––14.070.073–––L glycogen11.260.27210.990.33010.570.458WB glucose18.450.006*16.700.014*11.230.276WB lactate10.070.79010.150.70212.790.106L HSP70127.62<0.001*18.280.008*10.280.601L Ub10.230.63310.220.64010.300.588RBC HSP70116.88<0.001*1182.09<0.001*12.670.113RBC Ub10.120.73710.280.59810.350.558CTMax = critical thermal maximum; L = liver; M = muscle; WB = whole blood; RBC = red blood cell; HSP70 = heat shock protein 70; Ub = ubiquitin. Asterisks indicate significance of two-way ANOVA with α = 0.05 and P <0.05.**One-way ANOVA performed.