Calpastatin overexpression does not impact hemodynamic or renal blood flow. At one year, blood pressure, renal blood flow and glomerular filtration rate (GFR) were similar in CalpTG (TG) and WT mice, evidencing that calpastatin does not protect kidney through hemodynamic improvement (A–C, n = 6–10/group). Although hemodynamic features were similar, there was a trend toward lower heart mass in CalpTG (TG) mice at one year (D, p = 0.05, n = 7/group).