RT-PCR was performed by using PrimeScript One Step RT-PCR Kit Ver.2 (TaKaRa Bio) to confirm the sequences of the contigs obtained from the Kagoshima sample. Three primer sets were designed based on the contig sequences of this sample. Primer sequences are given in Additional file 1: Table S1. PCR products were sequenced using a 3130xl Genetic analyzer (Applied Biosystems, Foster City, CA, USA).