In summary, our results show that the use of hermetic storage mitigates many of the environmental effects that lead to the spoilage of grain. Hermetic storage provides a barrier to the exchange with environmental moisture, preventing rewetting that can contribute to the proliferation of storage fungi. Our results also support previous research showing that hermetic storage bags protect maize from insect infestation and development. Although conditions in the woven bags never became conducive for the spread of A. flavus, other storage fungi were able to proliferate throughout the woven bags due to optimal conditions of moisture. The placement of A. flavus-colonized and non-colonized maize in small satchel provided evidence that A. flavus spread and aflatoxin accumulation does not occur if the grain moisture content is not optimal. These results also suggest that protocols for sampling storage must account for high aflatoxin-containing kernels within the bags that can lead to variability and potential erroneous conclusions about the efficacy of hermetic bags. Ultimately, this study demonstrated the use of PICS bags as a practical option for storing and protecting grain from a variety of detrimental environmental effects.