After treatment with the conditioned medium from fibroblasts with SCF silencing, the melanocytes showed downregulation of MITF, ERK-1, ERK-2, Myo5a, and c-kit, while tyrosinase and Pmel17 were upregulated, compared with treatment with regular fibroblast medium. Under this situation, SCF was inhibited by siRNA, so the expression was followed by a decrease, and then the melanocytes treated with this medium showed decreased gene expression of ERK-1, ERK-2, and SCF receptor c-kit. Comparing regular fibroblasts exposed to UVB and SCF-inhibited fibroblasts treated with UVB stimulation, the melanocytes cultured in the conditioned medium showed increased gene expression of tyrosinase, MITF, Myo5a, and c-kit. According to the above results, HGF, NRG, and CRH were upregulated in SCF-silenced fibroblasts with UVB exposure compared with fibroblasts treated with only UVB, so the melanocytes accepted more stimulation to increase the melanogenesis-related gene expression level. SCF silencing also caused ERK-1 downregulation, but ERK-2 showed no significant change in its gene level compared to regular fibroblasts exposed to UVB in melanocytes. In addition, this result is observed when the SCF gene is knocked down, the gene Myo5a is downregulated, and Pmel17 is upregulated whether there is UVB exposure or not.