Fifteen micrograms of GPAT dsRNA was injected into the abdominal intersegment membrane of female pupae at 48 h before adult elcosion. The female pupae were maintained for 48 h until emergence under normal conditions. The newly emerged females were decapitated, maintained for 24 h and then injected with 10 pmol PBAN. The PGs were collected 90 min after the injection and then dissolved in hexane. Control females were injected with dsRNA of EGFP.