There was a significant difference between day 35 of protocol A as well as day 14 of both protocol B and C (P-value < 0.05). Significant differences also existed between protocol A at day 35 and protocol C at day 14 (P-value < 0.05) as well as between protocol B, day 14 and protocol C, day 14 (P values: <0.05). However there was believed to be no difference between protocol A day 35 as well as protocol B day 14 (P-value: 0.23). Results for DNA are demonstrated in Fig. 8.Figure 8Mean DNA content for different protocols (N = 3). Error bars for SD.