In a second experiment, we compared the effects of AAV-WT and AAV-2P on mouse behavior using the rotarod test (Fig. 2c). Again, as compared to the AAV-maxFP, AAV-WT induced a significant decrease in the latency to fall already at 1.5 months post-injection, and which remained stable at 3 and 7 months after vector injection (Fig. 2c). Remarkably, however, the presence of the two β-structure breaking prolines in tau (AAV-2P) reversed this effect, as the mice injected with the AAV-2P vector performed as well as the control AAV-maxFP mice in the rotarod test (Fig. 2c). Over the whole course of the experiment, their latency to fall was significantly increased for the AAV-2P mice compared to the group injected with AAV-WT (Fig. 2c).