H&E staining was performed to observe the morphological changes of cortex and hippocampus tissues in mice brain. The results showed that the morphology of brain tissues in the sham group was normal, and the cell density was higher. However, loose structures in hippocampal and cortical neurons with disordered hippocampal pyramidal cell layers occurred in the model group. Furthermore, the images showed that not only the number of neurons was reduced, but also that neurons staining were abnormal with nuclear condensation in the model group. DNLA treatment significantly decreased the number of abnormally stained neurons. Also, the morphology of brain tissues was generally normal in the DNLA group (Fig. 3).