To our knowledge, this is the first report to demonstrate that a repeated α-GalCer injection ameliorates podocyte injury, thereby inducing an anti-proteinuric effect. The number of WT-1 positive cells was significantly larger and the nephrin staining score was significantly higher in the α-GalCer group. Moreover, the preservation of podocyte foot processes was observed in the α-GalCer group. We assessed the proteinuria-free survival time in mice using two analysis methods; one showed a statistically significant difference, while another only suggested a tendency toward significance. Regarding this point, it has been reported that the log-rank test may show a tendency toward significant results25. Regardless, the daily levels of proteinuria in the α-GalCer group were significantly lower than those in the vehicle group.