A, B. Expression level of miR-145-5p and miR-5096 was measured by qPCR in HMEC (black) and U87 (dashed), cultured separately (left) or co-cultured (right) for 12 h (means ± SD; *P<0.05; n = 3). Levels of miR-145 or −5096 were measured relative to levels of U6 snRNA, as an internal control. For co-cultures, U87 were labelled with the fluorescent dye DiL-C18 (red cells), plated with unlabeled HMEC at a 1:1 ratio, and sorted by flow cytometry before analysis.