Our study applied IHC and qPCR to investigate the protein and mRNA expression of GC tissues. Moreover, qPCR also tested the mRNA expression of normal gastric tissues. These might be the reason why the results of IHC and qPCR were not correlated with each other. The results of Western Blot was similar to IHC, but not consistent with qPCR in some markers. We thought that the expression of mRNA in qPCR might differ from the expression of protein in IHC because of the changes after transcription and transduction. Additionally, our study only investigated the clinical significance of these markers, but did not involve the molecular mechanism of these markers. At present, these markers were mainly applied in identification of CSCs and investigation of their clinical significance. Regarding the mechanism, some study found that TR4-Oct4-IL1Ra axis might play a critical role in the development of chemoresistance in the prostate cancer stem/progenitor cells . GC cell migration was enhanced through increasing CD54 through Rho/ROCK pathway by leptin .