Prior miRNA expression profiling studies of human PDAC specimens demonstrated elevated expression of components of the mir-17~92 cluster in PDAC. However, the results from these studies were somewhat inconsistent, potentially reflecting the significant stromal and immune cell component of pancreatic tumors. To ascertain whether mir-17~92 miRNAs have elevated expression in PDAC cells, we profiled a panel of PDAC cell lines as well as the immortalized pancreatic epithelial cell line HPNE. We find that mir-17~92 miRNAs are consistently overexpressed in PDAC cell lines (Supplementary Figure 1). Thus, we set out to identify the role of this microRNA cluster in pancreatic tumorigenesis in vivo in genetically engineered mouse models.