To investigate the impacts of radiation, the response of the metabolome in B16 cells to radiation were analyzed. Though metabolome analysis was performed in many clinic tissues or biofluids [28, 29], using cultured cell samples as a metabolic research model has multiple advantages, as not only can one exclude individual differences in clinic samples and tumor tissue heterogeneity, but also they are stable and have a high controllability; therefore, cell samples are appropriate for preliminary studies .