The absorbance at 570 nm (A570) measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay can reflect the splenocytes viability. The A570 values of AHG groups did not decrease compared with those of normal control (NC) group (Figure 1). It was suggested that AHG at 0.5–50 μg/mL had no toxic effect on mouse splenocytes. On the contrary, AHG at 1–10 μg/mL significantly increased the amount of splenocytes, which indicated that treatment of AHG at low concentration could promote splenocytes proliferation responses.