MS strategies for ECM characterisation. Untargeted proteomics is appropriate for discovery experiments where no a priori information is available. When a delimited number of targets of interest are known a priori, targeted proteomics offers a robust method for detection and quantification. Novel MS methods such as a combination of higher energy collision dissociation (HCD) and electron transfer dissociation (ETD) allow for characterisation of complex PTMs including glycosylation. ZIC-HILIC, zwitterionic hydrophilic interaction LC; Pd, product-dependent; Alt, alternating.