In the mice injected with G131V brain, PrPSc deposition, spongiform degeneration and gliosis were observed in all eleven mice examined at timepoints starting at 531dpi. PrPSc was found mainly in the cerebral cortex and hippocampus. At 531 dpi, PrPSc appeared to be associated with axons giving a linear pattern in the cerebral cortex and a punctate pattern in the Oriens layer of the hippocampus (Fig. 4d-f). This difference in morphology appeared to depend on whether the plane of section was across or parallel to the axons involved. The linear pattern in cortex was similar to the morphology observed in the original patient. At 731dpi, the fine linear PrPSc pattern was still seen in cerebral cortex, but in the Oriens layer of the hippocampus, there were both large plaque-like deposits and smaller punctate deposits, which suggested more extensive axonal dystrophy and possibly extra-axonal plaque-like deposition. Despite the plaque-like size of some of these deposits, they did not stain with Thioflavin S suggesting they were not amyloid.