The mechanism of action of Wa1F1-KT on C. glabrata appeared to be independent from the fluconazole-resistance pathway, as only slightly different effects were observed against the susceptible clinical isolate (DSY562) or its mutant derivative strains (SFY93, SFY105, SFY115, SFY116). This phenomenon underlies the potential of Wa1F1-KT as a universally active anti-C. glabrata tool, likely not affected by drug-resistance phenotypes.