Variants identified by NOIR-SS were classified according to their presence within specific genes (Table 3). Filtered variants were defined as those selected by the CV78 filter. A characteristic common to the first and the second sample sets was the high recovery rate of KRAS mutations with the CV78 filter. This is due to mutational hotspots present within codons 12 and 13. There was no significant difference in the recovery rates (i.e., the percentage of selected variants) between the first and the second sample sets.