The plasmid of eight soluble phage ELISA positive scFv clones was isolated and sequenced, and we found 7/8 clones were similar in sequence because of the enrichment of antigen-specific clones after five rounds of biopanning. Therefore, two clones (2B10 and 2E4) out of eight identified scFv clones were distinct and were taken for further analysis. Analysis of the gene usage of the scFvs by IMGT/V-Quest software program revealed that both 2B10 and 2E4 scFvs are variants of the same immunoglobulin heavy chain gene family IGHV1-02*02, but having different D and J region alleles, i.e., IGHD6-19*01 and IGHJ4*02 for 2B10 and IGHD6-13*01 and IGHJ3*01 for 2E4. The CDRH3 and CDRL3 region of 2B10 is shorter (13 amino acids in CDRH3 and 12 amino acids in CDRL3) than CDRH3 and CDRL3 regions of 2E4 (18 amino acids in CDRH3 and 13 amino acids in CDRL3). Also, higher number of somatic hypermutations (SHMs) was present in the immunoglobulin heavy chain and light chain genes of 2B10 than 2E4 (Table 2).