The host-guest complexation of 2C-WP5A and trans-G was first investigated by 1H NMR experiment in D2O at 25 °C (Fig. 2b). Signals for the protons on trans-G were significantly shifted upfield in the presence of 1 equiv. 2C-WP5A. This result suggested that the formation of the threaded structure 2C-WP5Atrans-G, in which these protons of the guest molecule were shielded by the aromatic rings in 2C-WP5A. The Job’s plot was employed to find the binding stoichiometry of 2C-WP5Atrans-G, the top point of the curve was appeared at the molar fraction Xguest = 0.5 (see Supplementary Fig. S12), indicating the 1:1 binding stoichiometry between 2C-WP5A and G.