Following fixation in 4% PFA, the specimens were paraffin embedded and were then subjected to dehydration and rehydration cycles. Nuclei were subsequently stained with celestine blue-haemalum sequence and rinsed in distilled water. Subsequent staining was conducted with Hematoxylin for 5 minutes. Staining was then conducted in ponceau-acid fuchsin solution for 5 minutes. Specimens were later differentiated in 1% aqueous phosphotunstic acid for 10 minutes. Counterstain in light green was performed. Specimens were finally dehydrated and mounted on to slides and examined with light microscopy (EVOS, XL, Core) (n = 3).