Peptide mapping using reversed phase ultrahigh performance liquid chromatography coupled to UV-Visible and tandem mass spectrometry detection (LC-UV-MS/MS) was used to confirm the primary amino acid sequence. LC-UV-MS/MS was performed according to the method as described in publication 4 of this series . To confirm identity, one 150 μL vial fraction (prepared as described in ESM) was analyzed from rack 1 of each lot of RM 8671 (14HB-D-001, 14HB-D-002, 14HB-D-003). Method performance was evaluated on the day of analysis by parallel sample preparation and analysis of PS 8670. Data collection and analysis for each method was performed as described previously .