CaLC4707: To C terminally RFP tag Hhf1 in a cas5Δ/cas5Δ mutant, the RFP-NAT cassette was PCR amplified from pLC447 as described for CaLC4705 and transformed into CaLC2056. Correct integration at the C terminus of HHF1 was verified as described for CaLC4705. To C terminally GFP tag Dad2, the GFP-HIS cassette was PCR amplified from pLC38363 as described for CaLC4705 and transformed into cas5Δ/cas5Δ HHF1-RFP/HHF1. Correct integration at the C terminus of DAD2 was verified by as described for CaLC4705.