An additional experiment found that the spider Nephila pilipes produces stronger and tougher MA silk when fed a high protein diet than when deprived of protein . Subsequent WAXS analyses found that, like wind exposed spiders , changes in silk crystal density explains some of the enhanced strength . Nevertheless, unlike wind exposed spiders, a combination of changes in crystallinity and nanostructural orientation in the amorphous region were also prevalent . In both instances, the silk nanostructures seem to vary independent of MaSp1: MaSp2 expression, although only indirect measures (i.e. amino acid composition) of MaSp expression was made . Different mechanisms at different scales seem to be responsible for nutritionally induced spider silk property changes compared to wind induced property changes. However, no study has holistically examined the consequences of gene expression on silk proteins and protein structure and, ultimately, silk functional properties to understand the mechanisms inducing nutritionally induced property variation in spider silks at multiple scales.