Examined in mice aged 19–21 wk, glucose-induced excursions in plasma insulin were not different between βVps13cKO and control male and female mice (Fig. 7, A–D). Likewise, by analyzing fasting glucose and insulin levels, we observed no indication of a change in steady-state β-cell function (36) as assessed using homeostatic model assessment (HOMA2-%B; Fig. 7E), nor insulin in insulin sensitivity (HOMA2-%S; Fig. 7F) in males maintained on either RC or HFD. By contrast, a tendency toward a lower HOMA2-%B value (Fig. 7G), accompanied by a significant increase in HOMA2-%S, was apparent in female βVps13cKO mice vs. controls fed on RC, whereas these differences were not observed on HFD (Fig. 7, G and H).