Bacteriolysis mutants do not induce apoptosis. (A) Lactate dehydrogenase assays were performed infecting wild-type (WT) and AIM2−/− BMDMs with bacteriolysis strains (MOI of 1). Data are presented ± SEM of results from two independent experiments. (B and C) yvcJ::Tn (B) and pdhC::Tn (C) strains (MOI of 0.2) were grown in BMDMs and then enumerated for CFU at 2 and 5 h postinfection. Data are calculated as fold change between 2 and 5 h and representative of results of two independent experiments. The panels on the right depict the same data but graphed as CFU at 0.5, 2, 5, and 8 h postinfection (D) iIFNAR−/− macrophages infected with bacteriolysis mutants at a MOI of 10 and measured for caspase-3/7 activation 6 h postinfection. Data are presented as means ± SEM of results from three independent experiments. NS, not significant. Download FIG S5, EPS file, 1.8 MB.