For the assessment of lymphocyte subpopulations, relative percentage is usually used, but absolute counts can be also calculated. As interferon beta decreases the overall lymphocyte count, including absolute values of respective subpopulations, we selected relative values for the statistical analysis. Since only results obtained before a relapse or CDP were statistically assessed, no other treatment than interferon beta could have influenced the parameters, and treatment was the same in all patients. The results obtained from patients after therapy escalation (18 natalizumab, 3 fingolimod) and also after changing for copaxone (6 patients) were excluded from this part of our study. Also methylprednisolone pulses were the same in all patients and the treatment was given at least 30 days before baseline.