The actin gene target could be amplified from 21 of the 23 T. vaginalis isolates. All 21 amplicons had the expected length of approximately 1100 bp. The two remaining isolates could only be amplified by a higher primer concentration of 0.5 μM, instead of 0.3 μM. However, one of these clinical isolates did not yield an interpretable sequence and thus only sequences from 22 clinical isolates were utilized in the typing. Five different actin types (E, G, I, N and P) were identified according to the position and the number of cleavage sites, following the scheme proposed by Crucitti et al.  (Table 2). The most prevalent actin genotype was E, representing 50.0% of the isolates. The other genotypes were, in order of descending frequency, N (27.3%), G (13.6%) and I and P (each 4.5%).Table 2Number and position of restriction sites using HindII, MseI and RsaI restriction enzymesGenotypeNo. of isolates HindII MseI RsaI213273699185314518103190426878994E11××××××××G3×××××××I1××××××××N6×××××××××P1×××××××××× indicates the presence of a restriction cut site