Unilateral limb ischemia was surgically induced in CD1 mice. Next, mice were injected in their ischemic adductor muscle with 100 μg of exosomes from either the PF or plasma. A control group received PBS. (A) Representative color laser Doppler images of lower limb perfusion at baseline and at day 7 post-ischemia. (B) The blood flow recovery to the ischemic foot was calculated as percentage versus the contralateral foot (n = 20 mice/group). (C) Percentage of necrotic toes in animals treated with PBS, PF, or plasma-derived exosomes. **p < 0.01 versus PBS. (D) Representative immunofluorescent images of ischemic adductor muscle sections after staining with green fluorescent isolectin-B4. (E) Capillary densities in the ischemic adductors at 7 days post-ischemia induction (n = 8 per group). Average capillary density was determined from eight randomly selected high-power fields (magnification 20×). ##p < 0.01 versus PBS. (F and G) Let-7b-5p (F) and Tgfbr1 (G) expression in ischemic mouse adductors injected with PF exosomes or PBS (control); n = 5. *p ≤ 0.05 versus PBS. All values are mean + SEM.