5) In Figure 5—figure supplement 1D/E: removing the lower quartile (or more!) of flowering time data for the correlation analysis by simple assumption that these represent the non-transformed Nd-1 individuals of the segregating population is questionable. While this may be an attractive assumption, it negates the fact that each line pool may represent its individual set of variances (also present in ND-1 itself). This would at least require a spot check for the presence of transgenes in this pool or the percentage of “clean” Nd-1 individuals. In principle, a simple test by PCR would have provided a solid answer to that. As is, the authors should include these lines in the correlation analysis as the factual genotype is not known.