CaLC3952: To C terminally HA tag CAS5 in the GLC7 repressible strain, the HA-HIS1 cassette was PCR amplified from pLC57562 using primers oLC2161 in combination with oLC2162 and transformed into CaLC3932. Transformants prototrophic for histidine were PCR tested with oLC2163 in combination with oLC2164.