MS data acquisition was performed using the 10 most abundant precursor ions from the survey scan within the 300 m/z to 1800 m/z mass range for high-energy collisional dissociation (HCD) fragmentation. The target value was determined on the basis of predictive Automatic Gain Control (pAGC). Dynamic exclusion for selected precursor ions was set at 60 s. The resolution was set as follows: 70,000 at m/z 200 for MS scan and 17,500 at m/z 200 for HCD spectra. The normalized collision energy was 30 eV and the under fill ratio was defined as 0.1%. This ratio specifies the minimum percentage of the target value likely to be reached at the maximum fill time. The instrument was operated in peptide recognition mode enabled.