In summary we have shown that K2P channels are widely expressed within the adult mammalian retina, with the highest levels of expression detected within RGCs and Müller cells. Our data suggest that K2P channels likely perform important roles in retinal function and vision, and likely contribute significantly to the resting membrane potential and cellular excitability of retinal ganglion cells under a diverse array of physiological conditions. The prominent role of K2P channels in neuroprotective pathways45465689 offers new potential avenues of research into the treatment of retinal disease. Notably, K2P channels may represent valuable targets for manipulation of resting membrane potential and suppression of pathological increases in spontaneous spike firing rates observed from RGCs following retinal degeneration909192.