For an ideal anti-obesity therapy, it is preferable that the weight loss stems predominantly from fat rather than lean tissues. We found that supaglutide induced decrease in body weight which was accompanied with a reduction in epididymal fat, which represents part of visceral fat. However, the weights of interscapular BAT and inguinal fat were not significantly changed. In general, visceral fat was considered as “bad fat” since it was associated with insulin resistance and increased cardiovascular risk (Hocking et al., 2013; Bouchi et al., 2016). However, subcutaneous fat and BAT were considered as “good fat” given their beneficial effects on the metabolism homeostasis (Hocking et al., 2013). It is presumably the reduction of epididymal fat may partly contribute to the beneficial effect of Supglutide in improving energy metabolism in the obese mice. In contrast, our result showed that the weights of lower leg muscles (including gastrocnemius and soleus) were not altered by supaglutide, indicating that the reduction in body weight of the obese mice was not due to losing lean mass.