Four-week-old female Balb/c-nu/nu nude mice were obtained from the Shanghai Institute of Materia Medica at the Chinese Academy of Sciences. Mice were randomly assigned to either the control or experimental group, and 5 × 106 PANC-1 cells subcutaneously injected into the right hind limbs. Subcutaneous injections of 0.1 mL PA (2 × 1010/ml) and 0.1 mL PA-MSHA (2 × 1010/mL) were administered daily to the control and experimental groups, respectively. The mice were injected intraperitoneally with Nab-paclitaxel (10 mg/kg, twice a week) for 7 weeks. Tumor size was measured twice a week using a vernier caliper. The following formula was used to calculate tumor size (V): V = π/6 × (W2 × L), and the values used to generate tumor growth curves. After the experiment, tumor tissues were collected and proteins analyzed by western blotting.