We previously reported that the pharmacologic inhibition of 5-HT synthesis does not affect the acute anorexic effects of liraglutide in mice . The results of the present study further demonstrated that the pharmacologic inhibition of 5-HT synthesis did not affect the feeding suppression induced by liraglutide for 24 h after injection in mice. Moreover, the inhibition of 5-HT synthesis did not affect both the acute weight loss and maintenance of weight loss induced by liraglutide in mice. Thus, the feeding suppression and weight loss induced by liraglutide could be independent of 5-HT synthesis in mice.