To identify expression pattern of TPS genes, expression level in different tissues and abiotic stress treatments, transcriptome datasets of lotus was analyzed. The data sets obtained from GenBank include: rhizome apical meristem (SRS413495), rhizome elongation zone (SRS413496), rhizome internode (SRS414455, SRS414456, SRS414457, SRS414458, SRS414459), leaf (SRS413488, SRS413489, SRS413490, SRS413491, SRS413492), petiole (SRS413498, SRS413499, SRS413500, SRS413501, SRS413502), root (SRS412272, SRS412273, SRS412278, SRS412279, SRS412280). The library preparation methods and statistics of sequencing data were detailed in Kim et al. (2013). To take advantage of our in house transcriptome data, we analyzed the expression pattern of TPS genes in response to submerged and 5 mm Copper-treated conditions. Three-month-old lotus seedlings were used for submergence (24 h) and 5 mm Copper-treatment (24 h). To achieve submergence, seedlings were transferred to a plastic tank and water was added into the tank to a water depth of 20 cm from top of lotus. The sample without copper or submergence treatment was the control (Con). After the treatments, total RNA was isolated using the CTAB-LiCl method. For mRNA-seq, three sets of total RNA were used for cDNA library construction and sequencing (without replicates) at the Beijing Genomics Institute (BGI, Shenzhen, China), following the manufacture's protocols.