Data in this study has shown that trypsin can certainly be effective. Histological analysis with DAPI stain has demonstrated that cells were successfully removed by day 14 with a significant reduction in comparison to native. Findings were supported by the H&E results which demonstrated a similar pattern to the DAPI staining. This was statistically proven with DNA showing a significant reduction after 14 cycles to native (P < 0.05). Staining with Masson’s Trichrome showed that collagen was still present within the extra cellular matrix which is important since it’s vital to maintain the biomechanical properties as well as acting as a biochemical cue when it comes to directing cell behaviour19. In addition the mean Young’s Modulus was maintained post decellularization at the end of protocol B compared to native.