Frontal cortex tissue was dissected from E16 C57BL/6 J mouse embryos following standard methods. We performed AAV2/6 infections at DIV1 to induce transgene expression using a viral dose of 2.5E + 5 TU per well. For this experiment, we used a bicistronic vector construct co-expressing a nuclear GFP to identify the transduced neurons (AAV2/6-PGK-Tau:CMV-nlsGFP). To determine the effects of human tau variants in presence of vinblastine, AAV2/6-infected neurons were incubated at DIV4 during 24 h or 7 days with 1 nM vinblastine and fixed with 4% PFA at DIV5 or DIV11. AAV2/6-transduced mouse cortical neurons overexpressing human tau were immunostained using the HT7 (Thermo Fisher Scientific, MN1000) and GFP (Thermo Fisher Scientific, A-11122) antibodies. For morphological analysis, all conditions were performed in triplicate.