The SeqMan NGEN (DNASTAR) program for next generation transcriptome sequence data assembly was used to assemble contigs from the pooled 454 sequence data files for all five BBD-resistant tree libraries. Similarly, the pooled dataset consisting of 454 sequence data files for the five BBD-susceptible tree libraries was also assembled using the NGEN program. Finally, to obtain a reference transcriptome for F. grandifolia, all of the 454 sequencing data files for the ten libraries were pooled and assembled into a combined set of transcript contigs.