In primary lesions (n=93) tested by qPCR and IHC, we found that there were no relationships between IHC and qPCR in CD54 (p=0.477), Lgr5 (p=0.576), CD133 (p=0.792), Oct4 (p=0.834), EpCAM (p=0.630) and Sox2 (p=0.250). We have also compared the expression of these markers in some patients through Western Blot, the results of which was similar to IHC, but not consistent with qPCR in EpCAM, CD133, Oct4 and Lgr5 (Figure 3).