HUVECs were plated in 6-well plates. The cells were transfected with siControl (control siRNA, 20 nM, #AM4611, Thermo Fisher Scientific) or siKLF2 (KLF2 siRNA, 20 nM, #E006928, GE Dharmacon) in opti-MEM using RNAMAX (Thermo Fisher Scientific). After 6 h, the medium were changed with cell culture medium with 10%FBS and incubated for 48 h. Then the cells were treated with TNFα or TA (10 µM) as described in cell adhesion assay.