In B. mori, PG is formed at 72 h before emergence (−72 h), begins to produce sex pheromone at emergence (0 h) and maintains to release sex pheromone until 72 h after emergence (72 h). Thus these 3 time points (−72 h, 0 h and 72 h) in B. mori development were chosen for proteomic analysis. iTRAQ-based quantitative proteomic analysis was performed to determine the differentially expressed proteins at the different time points of PG development in B. mori. A total of 10117 peptides matched to 1523 proteins with scores higher than 1.3 (confidence level of 95%) and FDRs lower than 0.01.