Using qRT-PCR KRT5 is expressed by metastatic OVCAR-5, OV-90, and SKOV-3 ovarian cancer cells, as well as by poorly metastatic OVCAR-3 cells but not the peritoneal cell line, LP-9 (Figure 1A). KRT6C was expressed by all ovarian cancer cell lines as well as LP-9 cells (Figure 1B). The K5/6 antibody detected bands at ~52 kDa in all cell line extracts and faint bands at ~56 kDa in protein extracts from OVCAR-5, OV-90 and SKOV-3 cells (Figure 1C). Using human ovarian cancer tissue extracts shown to express high and low K5/6 and K5 positivity (see inserts in Figure 1C and 1D) and antibodies to only K5, we confirmed that the 56 kDa and 52 kDa bands were K5 and K6, respectively. Two bands at ~52 kDa and ~56 kDa were observed with the K5/6 antibody in the ovarian cancer tissue extracts (Figure 1C), however the K5 antibody (Abcam) only detected a single band at ~56 kDa in the ovarian cancer tissue (Figure 1D).