In S. cerevisiae, the activity of a N-terminally truncated form of the vacuolar alkaline phosphatase Pho8 (Pho8Δ60) is widely used to measure the magnitude of autophagy (Noda and Klionsky 2008). Normally, Pho8 is transported to the vacuole via the secretory pathway, where it is processed and activated to form the mature form by proteolysis. Upon removal of the N-terminal 60 amino acids, Pho8 can only be transported to the vacuole by autophagy. Thus, the amount of Pho8 enzymatic activity under these conditions is a measure of autophagy. We performed the Pho8Δ60 assay in the arl1Δ (YSA003) strain, containing the PHO8Δ60 allele after transformation with plasmids bearing the eight genes of interest (Figure 1B). ARL1 and empty vector (YEp352) were used as controls. The results show that, consistent with the GFP-Atg8 assay, overexpression of COG4, TAX4, SNX4, and ARL1 was able to suppress the autophagy defect of the arl1Δ strain at 37°, whereas overexpression of IVY1, ATG5, SLT2, and PEP3 was not.