The neutralization reactivity with V3-glycan N332 of the identified scFv monoclonals 2B10 and 2E4 was assessed by using human immunodeficiency virus type-1 subtype C pseudoviruses CAP256 wild-type and its mutant N332A using a starting concentration of 100 µg/ml of scFvs. Neither of the two scFv monoclonals shows N332A dependent neutralization activity.