(A) BAEC were pre-treated with Pazopanib at the indicated doses for 1 h, and stimulated with VEGF (100 ng/ml) for 10 min. Cell lysate was analyzed by western blotting. Endogenous VEGFR2 were immunoprecipitated and analyzed by western blotting against anti-phosphotyrosine antibody. (B) BAECs were exposed to Pazopanib at the indicated dose and time. Cells were fixed, and apoptosis was assessed using bis-benzimide staining. (C) After pre-treatment with Pazopanib (100 nM) for 16 h, BAECs were irradiated at the indicated doses. Cells were fixed at 8 h post-radiation, and apoptosis was assessed using bis-benzimide staining. Each value represents the mean ± SD of duplicate determinations from three separate studies. * p < 0.05.