In our study, TaFlo2-A1 was found to be associated with TGW and explained from 4.42% (in the Pakistani collection) to 8.37% (in the MCC) of phenotypic variation. The TGW-related QTL identified on 2AL includes ‘Xgwm339-Xbarc311’ in 139 RILs between two hard red spring wheat lines ; QTgw.ipk-2A (Xgwm372) in 111 BC2F3 lines derived from the cross ‘Flair × XX86’ ; QGwt.crc-2A (Xgwm558-Xgwm294) in a double-haploid population generated from the cross ‘RL4452 × AC Domain’ ; QTkw.sdau-2A (Xwmc181a-Xubc840c) in 131 RILs derived from ‘Chuan 35050’ × ‘Shannong 483’ ; QSZ.uaf-2A.1 (Xwmc455) in natural populations of 108 CIMMYT and Pakistani spring wheat accessions ; and QTkw.hwwgr-2AL (Xgwm312–IWA6090) in 127 RILs derived from ‘Ning7840’ × ‘Clark’ . Furthermore, three QTL on 2AL that were stable across five trials were detected in the same MCC (262) used in present study .