To detect changes in fungal alpha diversity, Chao1 estimations of diversity were applied to OTU distributions (Table 2). Fungal diversity increased distally with the greatest diversity in the hindgut of the mixed-diet fed fish. The opposite trend was observed in the wood-fed fish with the foregut having the most fungal diversity. These findings were supported by the rarefaction analysis, which demonstrated that the hindgut had the highest detectable species richness, while the foregut of mixed-diet fed fish had the lowest. The rarefaction analysis suggests that additional sequencing would allow more novel OTUs to be detected but the majority of the diversity present had been sampled (Figure 1). T-tests revealed there was no significant difference (P = 0.4) in the average number of fungal OTUs observed between wood or mixed-diet fish. Differences in Sobs and SChao1 suggest more unique or rare OTUs being present in the wood-fed foregut (Table 2) than any other tissue region examined.