The effects of AHG on mitogen-stimulated murine spleen lymphocyte proliferation were presented in Figure 4. Apparently, the proliferative responses of lymphocytes induced by both Concanavalin A (Con A) and Lipopolysaccharide (LPS) were reduced remarkably in CY-treated mice (p < 0.01) compared with the NC group. Combining with Con A or LPS, AHG could enhance the proliferation of splenocytes in a dose-dependent manner. The 5 and 10 mg/kg AHG combined with Con A significantly promoted and strengthened the proliferation of the splenocytes compared with that of MC group (p < 0.01). Similar result was also found in the stimulation of AHG combined with LPS on lymphocyte proliferation, while, in synergistic stimulation with LPS, AHG provided obvious increase of the splenocyte proliferation at 1, 5 and 10 mg/kg compared to the MC group (p < 0.05). Especially at the dose of 10 mg/kg, the A570 values were nearly equal to that of normal mice. These results indicated that co-mitogenic activities of AHG, with either Con A or LPS, were dose-dependent.