Sample sizes were determined in previous publications, and experiment types and are indicated in the legends. Representative results of at least two repeated experiments are shown. All experiments were performed with at least five biological replicates. Individual fish or pooled individual embryos were used as biological replicates. No data were excluded unless the animal died during the procedure. The Mann–Whitney U test and Fisher’s exact test were used. The statistical methods used and p values are indicated in the legends. Raw quantification data are available in Supplementary file 2.