Mitochondrial NRB-AF12 and ER-Tr localization was investigated in living LX2 cells transfected with the mitochondrial marker MitoDS-red plasmid, and then loaded with either NRB-AF12 or ER-Tr. As depicted in Figure 6, both dyes labeled mitochondria to similar levels (Pearson's Coefficient: 0.56 ± 0.05 and 0.54 ± 0.03, respectively for NRB-AF12 and ER-Tr; n ≥ 10).