Treg phenotyping. A, Gating strategies to isolate total Treg (CD127−/lowCD25+); naive Treg (CD127−/lowCD25intCD45RA+); CD25int mTreg (CD127−/lowCD25intCD45RA−); and CD25hi mTreg (CD127−/lowCD25hiCD45RA−). B, Percentages of cells with demethylated TSDR (n = 11) and (C) percentage of cells that express FOXP3 (n = 12) before expansion (Mann-Whitney U test, *P < 0.05, **P < 0.01, ***P < 0.001). Median with interquartile range is represented.