Transposon-induced mutants are preferred over mutants generated using irradiation or chemical mutagenesis approaches because they typically contain a very few mutations per mutant and therefore are easier to analyze and utilize in breeding programs. However, heterologous transposable elements have to be genetically transformed and at least for the maize Ac/Ds system requires the generation of a large collection of transgenic soybean lines because the element moves only to linked genomic regions . The requirement of tissue culture for the activation of Tnt1 limits its uses in functional analyses of soybean genes because tissue culture itself can generate new genetic and epigenetic mutations, broadly known as somaclonal variation. Furthermore, functional analyses of tens of thousands of soybean genes will require growing a huge number of transgenic soybean lines in the field with proper care not to release transgenes to the environment. Once a product is developed from such a huge effort, the seed industry must then wait to get approval for deregulating the transgenic soybean lines. Then, there is also the issue of non-acceptance of transgenic soybeans by a significant proportion of soybean consumers. Given the complexities associated with the use of heterologous transposons summarized above, endogenous transposable elements are better suited for functional analyses of soybean genes and the generation of useful mutants for breeding desirable cultivars.