Supaglutide injections twice a week for 4 weeks drastically reduced ectopic lipid accumulation in liver of the obese mice on HFD feeding (Figure 3A). This was associated with significantly decreased liver triglyceride contents compared with the non-treated obese control mice (Figure 3B, SPG vs. Ctrl = 12.7 ± 0.7 vs. 17.32 ± 0.47, p < 0.05). Furthermore, while the untreated control mice exhibited impaired liver function caused by HFD feeding as reflected by high ALT and AST content, supaglutide treatment significantly decreased the levels of the two enzymes (Figure 3C, ALT: SPG vs. Ctrl = 34.2 ± 7.7 vs. 153.4 ± 18.7, p < 0.01; AST: SPG vs. Ctrl = 72.20 ± 19.29 vs. 145.6 ± 16.8, p < 0.05). Figure 4 shows the morphologies of the brown adipose tissue (BAT), inguinal white adipose tissue (WAT) and epididymal WAT. Cell sizes in all fat depots tended to be smaller in the supaglutide group compared with the controls.