In IHC, there were 311 (82.5%) and 66 (17.5%) patients in CD133 low and high expression groups, respectively. CD133 high expression had obviously relation to age >60 years (p=0.001), upper parts of stomach (p=0.003), macroscopic type IV (p=0.047), well or moderate differentiation grade (p=0.014), N2 stage (p=0.016) and TNM III stage (p=0.018). In multivariate analysis, age (p=0.008, OR=1.769, 95%CI [1.162-2.692]), longitudinal location (p=0.022, OR=0.694, 95%CI [0.509-0.948]), differentiation grade (p=0.014, OR=0.600, 95%CI [0.401-0.900]) and N stage (p=0.003, OR=1.504, 95%CI [1.154-1.961]) were independently related factors.