Middle cerebral artery occlusion (MCAO) induces neuregulin receptor degradation protein-1 (Nrdp1) upregulation in cerebral cortex. Rats were subjected to 3-h MCAO before isolating hemispheric cerebral cortex. The mRNA and protein levels of Nrdp1 in cerebral cortex from nonischemic (Non-I) and ischemic (I) hemispheric tissue were analyzed by real-time RT-PCR and western blot. (A) Real-time RT-PCR analysis showed that Nrdp1 mRNA expression was significantly increased in ischemic hemispheric cortex. *P < 0.05 vs. Non-I, ANOVA; n = 6. (B) Western blot analysis revealed increased levels of Nrdp1 protein in ischemic hemispheric cortex. Upper panel: representative immunoblots of Nrdp1 and the loading control β-actin; bottom panel: quantitative data of protein band intensity after normalization to β-actin. *P < 0.05 vs. Non-I, ANOVA; n = 6.