In the study of Anstett et al., tissue culture selections with DTG, using viruses resistant to first-generation INIs (RAL and EVG), infectivity and resistance assays were performed. They showed that the presence of the E92Q or N155H resistance mutations was compatible with the emergence of R263K, whereas no R263K selection was observed in presence of G140S-Q148R, E92Q-N155H, G140S, Y143R and Q148R resistance mutations . Thus, some genotypic resistance profiles seem to prevent emergence of the R263K DTG resistance-associated mutation.