COACH  was used to predict calcium-binding sites in the N-terminal region of CsTegu20.6, based on the identification of analogs with similar binding sites. GalaxySite  was employed to predict specifically binding non-metal ligands and their binding conformations for screening putative inhibitors of CsTegu20.6. The detailed information regarding specific protein-compound interactions such as hydrophobic interactions, was visualized using LigPlot+  implemented in GalaxySite. ADMET properties were evaluated using admetSAR . Lipinski’s rule of five and binding energies were calculated using the Molinspiration online server (http://www.molinspiration.com/cgi-bin/properties) and MTiAutoDock , respectively.