In order to determine if KDM2 family members are transcriptionally regulated in hypoxia, U2OS and HeLa cells were exposed to 1% O2 for 24 h prior to mRNA extraction and qPCR analysis. Levels of KDM2A and KDM2B were analysed (Figure 1A,B) and CA9, a known HIF-dependent target, was used as a control (Figure 1C). Both KDM2A and KDM2B mRNA were induced by hypoxia (Figure 1A,B). As expected, CA9 mRNA was robustly induced in both cell lines following hypoxia (Figure 1C). In addition, we also investigated if hypoxia was able to induce KDM2 in the model organism Drosophila melanogaster, where only one gene is present. Here, whole flies were exposed to 5% oxygen for 24 h prior to mRNA extraction and gene expression analysis. As it can be seen in Figure 1D, hypoxia induces the levels of KDM2 mRNA in this organism as well.