Sections were visualized using a Zeiss LSM510 Meta confocal microscope (lasers: 405, 488, 561 and 633 nm; Carl Zeiss) and Zeiss LSM META software. Images were taken sequentially with different lasers using × 10 (numerical aperture (NA) 0.45) and × 20 (NA 0.8) dry objectives, and × 63 (NA 1.4) oil immersion objective. Single images were collected using the × 10 objective and Z-stacks were collected at 4.5 and 2.5 μm for × 20 and × 63 oil objectives, respectively.