Visual stimulation based VR behavioral tests have been extensively used for memory evaluation [10–16,30]. Our results confirmed that the visual memory could last for at least 3 weeks after 3 weeks of training. When mice were reintroduced to the VR behavior test after a 22 day “gap,” they were able to achieve a similar level of performance as before the no-training break. In addition, they were able to quickly surpass the performance of the previous training session after additional training. Therefore, not only could the mice maintain some of their skill set before the break, but also the previous experience could facilitate the additional training. This memory capability is particularly useful to evaluate visual function of animals before and after the induction of a disease or treatment.