Effects of Nrdp1 expression on hypoxia inducible factor-1α (HIF-1α) and USP8 in vitro after OGD treatment. (A,B) Primary rat cerebral cortical neurons and PC12 cells were infected with Ad-si-control or Ad-si-Nrdp1 and exposed to 6-h OGD respectively. Western blot analysis of protein levels of HIF-1α and USP8 of ischemic neurons (top panels). Histograms show relative intensity of HIF-1α and USP8 (bottom panels). *P < 0.05 vs. Ad-si-control. #P < 0.05 vs. Ad-si-control + OGD; n = 4. (C,D) Primary rat cerebral cortical neurons and PC12 cells were infected with Ad-control or Ad-Nrdp1 and exposed to 6-h OGD respectively. Western blot analysis of protein levels of HIF-1α and USP8 of ischemic neurons (top panels). Histograms show relative intensity of HIF-1α and USP8 (bottom panels). *P < 0.05 vs. Ad-control. #P < 0.05 vs. Ad-control + OGD; n = 4.