By contrast, when DSIs were calculated from the velocities optimal during cooling (see Preferred velocities), most cells (8/10) exhibited greater DSIs. In most of them (6/8), the increases exceeded 20% (Figures 4A,B,Ei). Not surprisingly, the mean DSI during inactivation at 69% (±28.45; range: 18.2%–100%; n = 10) was substantially higher than that (44.25 ± 35.1%; range: 9.6%–100%) before inactivation. Note however, that after rewarming of PTV, the DSIs at stimulus velocities preferred during cooling remained quite similar to those during PTV cooling (Figure 4Eii).