First, all variables were subjected to a one-way ANOVA with repeated measures and the time step as a factor covariate. In order to control the false discovery rate (FDR) , p-values were adjusted using a Benjamini-Hochberg (BH) approach (Table 1). Variables with an adjusted p-value (FDR <0.05) were then subjected to 3 paired t-tests to assess the difference between t=0 and t=+1, between t=0 and t=+4 and between t=0 and t=+24. The full list of p-values was adjusted using a BH approach (Fig. 1). Fig. 1Mean evolution of the biological variables over time. Vertical bars correspond to + and - SEM at each point Table 1Reference values (at t=0) for the biological variables, birth weight and weaning weight (n=120)UnitsMinMaxMeanSEM F Tympanic temperature°C36.10040.25739.1680.050258.110White cellslog10(G/l)0.4911.4721.1880.011572.970Lymphocytes%46.60091.90067.4770.555112.180Monocytes%3.90016.2008.5570.19169.500Granulocytes%2.50035.60022.6080.52778.210 L/G ratio1.35536.7603.4660.29564.650Red cellsT/l1.4907.3305.1630.05469.420Mean corpuscular volumefl39.70063.70052.0080.33362.120Hematocrit%6.80037.40026.8280.29978.990Hemoglobing/dl6.90012.8008.9470.09246.680Red blood cells distribution widthfl29.10033.80032.0290.08174.440Plateletslog10(G/l)2.3302.9982.6670.011227.400Mean platelet volumefl7.60013.0009.6820.10271.210Platelet distribution width%9.60012.00010.7710.045122.790Cortisollog10(ng/ml)1.0412.0331.4750.017370.240Free fatty acids \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$\sqrt {(\text {mmol/l})}$\end{document}(mmol/l) 0.0790.5600.1620.005111.040Glucosemmol/l5.8509.5258.0350.061123.990Bilirubin μmol/l4.66013.0008.5230.190178.610Birth weightkg0.4002.6801.4920.033Weaning weightkg5.46016.5649.4860.174Results of the ANOVA for time effect (F value): all variables varied significatively during the experiment, with an FDR <10−12, except for the weights (not tested because constant)