NK cells and CTLs are two major populations of cytotoxic lymphocytes, and play important roles in the control of tumor growth and metastasis . In this work, IL-2 (also termed T-cell growth factor) was used to enhance the generation and cytotoxic activity of NK and CTL cells in vitro . NK cells can efficiently kill cells without previous sensitization. Obviously, treatment with AHG dose-dependently accelerated the cytotoxicity of NK cells to Yac-1 lymphoma cells in this study. DCs are the most potent professional antigen presenting cells that can induce antigen-specific CTL immune responses and regular adaptive immune response, as well as participate in differentiation of T cells subset and inherent immunity response . Tumor lysate-pulsed DCs presented tumor antigens to T cells by both major histocompatibility complex (MHC) class I- and class II-pathways, and then provide the potential to induce efficient antitumor immune responses . Flow cytometry analysis showed that these DCs, which expressed high levels of CD80 antigen, had typical mature phenotypic markers. Functionally, these cells gained the capacity to stimulate allogeneic T cells. The results indicated that primed T cells in vitro with B16 melanoma cells lysate-pulsed DCs were able to induce specific CTL against B16 tumor cells. Especially, after stimulated with various concentration of AHG, the cytotoxicity of specific-CTL were much stronger than that in NC group. Taken together, it was demonstrated that AHG can significantly promote NK cells and specific-CTL antineoplastic activity.