Mice were anesthetized with a mixture of ketamine (100 mg/kg; Imalgene 1000, Merial) and xylazine (10 mg/kg; Rompun 2%, Bayer) for ABR analysis using a Tucker Davis Technologies workstation. The electrical responses to broadband click and 8, 16, 20, 28 and 40 kHz pure tone stimuli, with an intensity range 90–20 dB SPL in 5–10 dB steps, were recorded as previously reported (Cediel et al., 2006; Murillo-Cuesta et al., 2012). The electrical responses were amplified and averaged to determine hearing thresholds for each stimulus. Peak and interpeak latencies were analyzed at 15–20 dB SPL above hearing threshold after click stimulation. Recording of distortion product otoacoustic emissions (DPOAEs) was performed after stimulation with f1 and f2 primary tones, with a ratio f2/f1 = 1.2 using a TDT equipment, as described previously (Martinez-Vega et al., 2015b). Primary tones for 8, 10, 14, 18 and 22 kHz frequencies were tested.