DEAD box RNA helicase 20 (DDX20), forkhead transcription factor 2 (FOXL2), and fushi tarazu factor 1 (FTZ-F1) were investigated in the regulation of vitellogenesis (VTG). FOXL2 negatively regulates the VTG synthesis at developmental stage. FOXL2 downregulates VTG's expression by binding DDX20 in the regulation of follicular cell apoptosis and repress the synthesis of VTG when bound with FTZ-F1 at the mature stage [18, 34, 35]. According to our research, during the process of testis development, FOXL2 had no obvious change, and FTZ-F1 gene was downregulated. Therefore, FTZ-F1 may negatively regulate the testis development. Furthermore, cytochrome P450 upregulated significantly in the SP indicates that the mechanism of cytochrome P450 regulating gonad development in fish and crustaceans may be completely different.