Analysis of the phosphorylation status of AKT at the Thr308 and Ser473 sites that are phosphorylated by PDK1 and mTORC2 also did not show any differences between genotypes (Figure 2I–2P). Taken together with the phosphorylation of MEK, these data suggest that the alterations in ERK phosphorylation do not reflect an overall reduction in KRAS signaling, and further suggest that mir-17~92 regulates PanIN maintenance by specifically influencing ERK pathway activity downstream of KRASG12D and MEK.