LPS is also known as lipoglycans and endotoxins. The molecules is a component of the outer membrane of the cell wall of gram-negative bacteria, and is one of the most powerful bacterial virulence factors in the pro-inflammatory response . Initiation of the inflammatory response by LPS stimulates a strong immune response. LPS acts as the prototypical endotoxin through binding to the CD14/TLR4/MD2 receptor complex in many cell types, but especially in monocytes, dendritic cells, macrophages, and B cells, which promotes the secretion of pro-inflammatory cytokines, nitric oxide, and eicosanoids . Therefore, LPS is a central molecule in the pathogenesis of certain bacterial infections . In this study, in vitro co-culture with gingival epithelial cells and plaque bacteria caused pH changes of the culture medium during 24 h of incubation. Therefore, LPS was extracted from DPBs and its capacity to induce inflammation was verified. DPB-LPS significantly increased the levels of inflammatory factors in gingival epithelial cells, gingival fibroblasts and macrophages. EETC also effectively suppressed the levels of DPB-LPS-induced inflammatory factors.