Generation of heterozygous embryos on a C57/BL6 background, carrying floxed alleles of Vps13c was conducted by GenOway (France). Vps13cfl/fl mice were crossed to mice expressing Cre recombinase under the control of the Ins1 promoter (33, 69) to generate mice in which exon 1 of the Vps13c gene was selectively excised in pancreatic β-cells. Mice were born at the expected Mendelian ratios without any obvious physical or behavioral defects. Mice were housed two to five per cage in a pathogen-free facility under a 12:12-h light-dark cycle and had ad libitum access to water and standard mouse chow diet (Research Diet, New Brunswick, NJ). High-fat diet (HFD, 60% wt/wt fat content; Research Diet) was introduced at 4 wk of age.