Fifteen micrograms of GPAT dsRNA was injected into the female pupae at 48 h before adult elcosion. The female pupae were maintained for 48 h until emergence under normal conditions. PGs were collected and rinsed in PBS. The lipid were extracted by using chloroform: methanol (v:v, 2:1) and then were used for TAG determination using a TAG assay kit (Rong Sheng Biotech Co., Ltd, Shanghai, China) following a previously described method31. Control females were injected with dsRNA of EGFP. Corresponding results were compared using ANOVA and Tukey's test.