We next wished to characterize the mechanism by which miR-128 regulates telomerase activity. First, we performed RT-qPCR analysis of HeLa cells in order to determine if miR-128 acts by regulating the amount of TERT mRNA. Induced miR-128 expression significantly decreased TERT mRNA levels, relative to miR controls, whereas miR-128 neutralization by anti-miR-128 resulted in an enhanced amount of TERT mRNA, compared to HeLa miR control samples (Figure 2A). In addition, miR-128 overexpression resulted in significant degreased TERT mRNA levels in a teratoma cell line (Tera-1 or Tera) and in two iPSC cell lines, relative to miR controls (Supplementary Figure 1). anti-miR-128 showed a similar tendency as seen in HeLa cells, though less substantial in the iPS cell lines (Supplementary Figure 1).