The present study revealed a new family composed of two parents and three siblings. The parents were Bombino bianco and Uva rosa antica, a previously unknown variety found near Ricigliano, in the Montain townships “Tanagro- Alto e Medio Sele”, province of Salerno. The three siblings were: the renowned Uva di Troia, the Bombino nero and the Impigno (Fig. 1). Values of all 54 nuclear and 5 chloroplast microsatellites are reported in Table 1. As a confirmation, 6 more nuclear microsatellites were additionally analyzed, but only in the five members of the new discovered family, and their values are reported at the bottom of Table 1. Only one of the 60 microsatellites used in this study showed discrepant values for the proposed kinship. VChr-17a was in homozygosis at 189 pb in Bombino bianco, and at 179 pb in Uva rosa antica: only Impigno had heterozygosis 179/189 pb at this locus, while Uva di Troia and Bombino nero had not the 179 pb allele as expected. As already showed (Bergamini et al. 2013; Gasparro et al. 2013) the VChr-17a microsatellite is strongly affected by null alleles as denoted by the much lower observed heterozygosity frequency (0.308411) compared to the expected heterozygosity frequency (0.501834): the estimated frequency of null alleles is as high as 0.12879 and therefore the Uva rosa antica very likely has a null allele and this allele was inherited by Uva di Troia and Bombino nero.Fig. 1Pedigree of the proposed familyTable 1Molecular data: The genotype of 60 SSR loci and five chloroplast SSR loci of the analyzed varieties for the proposed kinship and of Pinot Noir as reference for size calibrationSSR loci Uva rosa antica Quagliano Bombino bianco Uva di Troia Bombino nero Impigno Pinot Noir VVS2133–143145–151143–151133–145133–145137–151VVMD5225–225227–231225–231225–227225–227227–237VVMD7238–242248–252242–252238–252238–248238–242VVMD25239–267241–263263–267239–241239–241239–249VVMD27178–188180–194188–194178–180178–194184–188VVMD28249–259245–249245–249249–249249–249219–237VVMD32240–250258–272250–272240–258250–272240–272VrZAG21191–205191– 191191–191191–205191–191201–207VrZAG62185–187189–199187–199185–199185–189187–193VrZAG79250–254250–258250–250254–258254–258240–244ISV2166–170166–170170–170166–166166–170152–166ISV3134–140140–146140–140140–140140–140134–146ISV4189–199189–193189–199189–189189–189171–179VMCNG4b9162–164150–176164–176162–176162–176158–162VChr-1b96–108100–10096–10096–100100–108100–108VChr-2b116–124116–124116–124116–124116–124124–124VChr-4a182–199182–199199–199182–182182–199182–199VChr-5b202–218202–206206–218202–202202–202190–190VChr-5c104–12888–12088–128104–12088–10496–116VChr-6a182–186182–182182–182182–186182–186182–186VChr-7b181–185181–189181–185181–186181–189181–189VChr-9a90–118109–11590–11590–115115–11890–98VChr-10b134–134138–144134–138134–144134–144138–144VChr-11b155–160157–164160–164155–157155–164152–164VChr-12a137–144144–144144–144144–144144–144137–144VChr-13a141–157149–157157–157141–149149–157157–157VChr-15a157–157149–149149–157149–157149–157153–157VChr-16a110–114114–114110–114110–114110–114102–164VChr-17a181–// 189–189189–//189–//181–189189–189VChr-18a164–168152–160160–168152–164152–168164–168VChr-19a141–141138–141141–141138–141138–141123–138VVI-b01298–298294–298294–298294–298294–298292–298VVI-b63150–154126–154126–154126–154150–154148–148VVI-b94292–306284–292292–306292–306284–306284–296VVI-f52262–274260–260260–262260–274260–262284–286VVI-h54166–168166–174166–166166–174166–166164–168VVI-i51253–265263–263253–263263–265263–265265–267VVI-m10357–371369–379371–379357–379357–369357–371VVI-m11291–301287–301287–291301–301291–301291–291VVI-m25167–169177–177171–177169–177167–177167–187VVI-n54100–106106–112106–112106–106106–10692–96VVI-n61361–375349–371349–361361–371349–375371–377VVI-n94278–289286–289278–289286–289289–289289–289VVI-o55141–143143–147141–147141–147141–147143–147VVI-p25b342–360340–362360–362340–342360–362362–362VVI-p37150–156144–148144–150148–156144–150140–150VVI-p77172–182186–190172–190182–186172–190180–190VVI-r09258–260258–264258–264258–260258–258242–248VVI-s21284–284284–290284–284284–290284–284270–284VVI-s58304–308304–308308–308304–308304–304294–294VVI-s63190–212190–212190–212190–190190–212190–190VVI-u04182–189182–191182–191182–191182–189166–170VVI-v37159–167159–167159–167159–167167–167149–159VVI-v69271–271257–271257–271257–271257–271289–291ccSSR-5256255255255255256ccSSR-14201202202202202201ccSSR-23285286286286286285cpSSR10110111111111111110NTCP8250250250250250250VChr-3a190–190187–199190–199187–190187–190202–202VChr-8b121–136103–106106–121103–136103–136103–139VrZAG112231–235237–245231–237231–237231–237243–245VVI-b23305–323305–311305–305305–305305–305309–331VVI-b6689–8989–10989–10989–10989–109103–109VVI-v61169–190169–192169–190169–192169–190169–169The last six loci were analyzed only in the five members of the proposed family. Allele lengths are in bp. ND: not detected