The purpose of this study was to describe the 20-year incidence (20 G and MIVS), causative organisms, and visual outcomes associated with endophthalmitis after pars plana vitrectomy from a single, tertiary eye care center in India. Furthermore, to identify the differences in clinical presentation between post-cataract surgery endophthalmitis and post-pars plana vitrectomy endophthalmitis, we compared our data with previous post-cataract surgery endophthalmitis studies after cataract surgery from India. To the best of our knowledge, this is the largest case series of incidence of post-vitrectomy endophthalmitis reported in literature.