Proteins from WT and CalpTG tissues were extracted in RIPA lysis buffer and protease inhibitor cocktail (1 µg/ml, Sigma). After homogenization, the lysate was centrifuged at 1000 × g for 1 h and the supernatant was frozen at −80 °C. Protein concentration was measured using the Bradford method. 20 µg of protein was separated by electrophoresis on a Bis-Tris gel 4–12% (Novex, Thermofisher). After proteins were transferred onto PVDF membrane, aspecific sites were blocked in PBS Tween and 5% milk solution before incubation with primary antibody overnight at 4 °C. Membrane was washed, then incubated for one hour with secondary antibody conjugated with peroxidase. ECL (RPN 3222, GE Healthcare life sciences) was applied 5 mn on membrane for chemiluminescent reaction. Reaction was revealed on radiographic films (Films were scanned onGS-800 Calibrated Densitometer) or read in Syngen Pxi imager (Ozyme). Optical densities were measured using the software Image J or with GeneSys software (Ozyme). Primary antibodies used were mouse monoclonal antibody anti-spectrin alpha chain (nonerythroid) (MAB1622, Chemicon Millipore, 1/1000), rabbit polyclonal antibody anti-calpain 1 domain IV, (ab39170, Abcam, 1/4000), rabbit polyclonal antibody anti-calpain 2 amino terminal end domain I (ab 39167, Abcam, 1/2000), rabbit polyclonal antibody anti-GAPDH (G9545, Sigma Aldrich, 1/50000),. Secondary antibodies used were anti mouse IgG antibody (NA931V, GE Healthcare, 1/10 000); anti rabbit IgG F (ab 1) 2 fragment antibody (NA9340V GE Healthcare, 1/4000), goat polyclonal antibody to rabbit Ig Secondary G-H & L (ab 6721, Abcam, 1/5000).