Biofilms are poorly investigated as a source of chronic inflammation and multi-resistant bacteria in biofilms and chronic organ dysfunction are huge clinical problems. The lack of scientific evidence demonstrating a near association between silent organ dysfunction and an infectious focus is a major problem in clinical medicine. We have attempted to produce antibodies using memory cells of patients that had undergone a severe infection caused by a pathogen in order to detect pathogens in polymicrobial environments. In future studies, we aim to identify and produce antibodies against epitopes that reflect the presence of pathogenic key bacteria in biofilms.