Matrix gene sequencing of A/New York/39/2012 (H3N2) FR-1307, A/Texas/50/2012 (H3N2) FR-1210, A/Switzerland/9715293/2013 (H3N2) FR-1368 and the Gainesville environmental 2013–2014 isolate A/environment/Gainesville/01/2014(H1N1) KJ195788 showed the same 3 bp mismatches in the capture probe for the H3N2 strains as opposed to only 2 different ones for the H1N1 when compared with the sequence of the proprietary GenMark RVP assay. Discussion of the location of these mismatches with GenMark suggested the 3 mismatches in the H3N2 strains were likely to be more destabilizing than the 2 mismatches in the H1N1 strain. Please see Table 2 for complete details. Since the GenMark assay sequence details are proprietary, specific sequence data as to which mutations were observed is not available. In contrast, the subtype genes of both the H3N2 and H1N1 strains have a perfect sequence match with their respective subtype capture and signal probes and a single bp mismatch in both forward and reverse primers.