Nowadays, TNM stage revealing tumor invasion depth, regional metastatic lymph nodes (LNs) and distant metastasis is one of the most important classifications of tumor progression and a useful clinical tool in prognostic prediction of GC [1, 9]. Nevertheless, TNM stage cannot illustrate complete information of tumors and patients. As far as we know, heterogeneity extensively exists in many tumors [10, 11]. In clinical practice, some patients with the same TNM stage are found to have different prognosis. Therefore, it is necessary to find new tools or crucial supplementary of TNM stage that represents the individual characteristics to accurately predict the prognosis of patients with GC. The aim of this study was to investigate the clinical significance of these six markers of CSCs and establish a new model based on these markers to predict the prognosis of patients with GC.