Only a few studies have investigated the bacterial induction of EGR1 in host cells. To determine whether the induction of EGR1 is a general stress response of the host cells to bacterial colonization, we performed a screen using 25 different bacterial strains including both Gram-positive and Gram-negative pathogens and non-pathogens. We divided the bacterial strains into groups depending on the site of isolation. Bacteria isolated from the upper respiratory tract were added to FaDu pharyngeal epithelial cells (Figures 1A,B), gastric isolates were added to AGS gastric epithelial cells (Figure 1C), and intestinal isolates were added to intestinal epithelial Caco-2 cells (Figure 1D). Bacterial isolates from the cervix were added to cervical ME180 cells and are shown in Figure 1C.