We developed a TaqMan PCR for conserved regions of the matrix gene that had no sequence variations in the primers and probe when matched against an environmental H1N1 strain from 2013 to 2014 season isolated at the University of Florida by one of the authors (JL) (Influenza A virus (A/environment/Gainesville/01/2014(H1N1) KJ 195790), and against sequences of A/New York/39/2012 (H3N2) FR-1307, A/Texas/50/2012 (H3N2) FR-1210, and A/Switzerland/9715293/2013 (H3N2) FR-1368 obtained from IRR/ATCC. The sequences for the assay are as follows:Forward primer 89-S: CCG AGA TCG CGC AGA GAC.Reverse primer 239-AS: GCT CAC TGG GCA CGG TG.Probe 177-AS-Pr: ATT GGT CTT GTC TTT AGC CAT TCC ATG AGA G.