The frequency of ISSR loci in populations of A. flaccida was variable, with the populations of Marialva PR and Porto Barreiro PR indicating higher deviations in the number of loci and consequently, lower variability (Fig 1). This graphical representation of the distribution of genetic variability is presented for the first time in this study, and when we evaluated the numerical indices that represent the genetic variability and differentiation between the populations (Tables 4–6), we observed that the results were the same (high variability and differentiation between populations). This new form of presentation genetic variability results is an efficient and more visually friendly way to demonstrate genetic differentiation between populations.