Recombinant adenoviral constructs, including Ad-control (control construct), Ad-Nrdp1 (overexpressing Nrdp1), Ad-si-control (expressing non-targeting control siRNA) and Ad-si-Nrdp1 (expressing Nrdp1 siRNA) were generated as described previously (Zhang et al., 2011a). Eight days after plating, cells were infected with Ad-control, Ad-Nrdp1, Ad-si-control or Ad-si-Nrdp1 for 24 h before OGD treatment.