We prepared our own compressive stimulation device as shown in Fig. 3. It can control applying load with 10 % strain elongation to the scaffold. The applied load was 19.6 Newton. The cells (1.8 × 105 cells/80 μl) were seeded onto the control and experimental specimen groups. The compressive stimulation was applied after 1 day from cell seeding to prevent the loss of cells from the specimens. The cells were seeded into the inner pore of specimens to prevent the damage of cells by compression.Fig. 3An apparatus for cell culture with compressive stimulation. The apparatus was manufactured to apply 10 % compressive strain to the scaffold in 12-well plate