For morphometry, a Bioval® optical microscope was used, with the program ToupTekView® version ´86 3.7.2270 (copyright 2003–2013; http://www.touptek.com) and the Opticam® 14 MP digital camera. The percentage of myointimal area was calculated by using the ImageJ 64 program, where the image of the vessel was formatted as follows: