10.7554/eLife.23644.003Figure 1—figure supplement 1.Negative stain and cryoEM of PLY solubilized in DDM, Cymal-6 and Amphipol.PLY rings solubilized with DDM vary in size and tend to orient on the specimen support or the air/water interface. Rings solubilized with Cymal-6 are more homogenous in size but tend to stack in pairs. Exchange of detergent against Amphipol A8-35 results in uniform, randomly oriented, single PLY rings. Scale bar: 50 nm.DOI: http://dx.doi.org/10.7554/eLife.23644.003