As shown in Figure 6A, C, and F, an increasing amount of Sbp1 leads to a 20%–60% decrease in the cap-dependent translation initiation activity of 5′UTRPab1. Similar changes in the cap-independent activity of 5′UTRPab1 were also observed. In contrast, the cap-dependent translation initiation activities of 5′UTRPab1-Δpoly(A) remain nearly unchanged when the concentration of Sbp1 increases.