In this study, three BEVs isolated in Japan, BEV IS1/Bos taurus/JPN/1990 (BEV-IS1) and IS2/Bos taurus/JPN/1990 (BEV-IS2), were additionally sequenced and analyzed. These viruses were isolated from a fecal sample collected from one cow at the same time in 1990 in the Ishikawa prefecture (The clinical features of cattle infected with BEV-IS1 and IS2 have not been recorded). In addition, BEV Ho12/Bos taurus/JPN/2009 (BEV-Ho12) was isolated from diarrheic feces collected in Hokkaido in 2009 by as described above .