After siRNA transfected into the intracellular environment and induced cellular SCF gene inhibition, the first aspect to confirm was whether the gene silencing caused cell death or a morphological change, since SCF was responsible for cell survival and proliferation. In MTT assay data, it presented no significant changes in fibroblast cell proliferations, and similar results were observed regarding fibroblast morphology, where without cellular atrophy damage, flattened or low confluency were observed. Fibroblast proliferation did not rely on SCF, even though it was silenced. Although SCF inhibition did not cause fibroblast death, the influence on fibroblasts was unclear. To explore the effect on fibroblasts when the SCF gene was silenced, some paracrine factors secreted by fibroblasts showing effects on melanocyte cell survival and melanin production were determined, including HGF, DKK-1, NRG-1, CRH, and ET-1.