(A) Quantitative analysis of mRNA expression of Ilrn (IL-1RA) and Il4ra (IL-4RA) cytokine receptors in GBC-PC. Data are presented relative to basal levels in control pericytes at each time point of culture, and normalized to the housekeeping reference gene expression. Results are mean ± Standard Deviation obtained from at least, four independent experiments, * p<0.01. (B) Flow cytometry analysis in control PC or GBC-PC for PDL-1 expression after 72 h. Gating strategy for GFP+ pericyte population for further flow cytometry analyses is shown (upper panel). Solid and dotted histograms represent staining with PDL1 specific antibody and isotype control, respectively. Insert numbers inside histograms represent mean fluorescence intensity values. The bar graph represents percentages of expression of PDL-1 in PC compared to percentages in GBC-PC. (C) Flow cytometry analysis of expression of the co-stimulatory molecules CD80 and CD86. (D) Flow cytometry analysis of expression of MHC-II (I-A). Non-specific fluorescence was measured using specific isotype monoclonal antibodies and GBM cells were used as negative controls. All data of flow citometry represents mean ± Standard Deviation obtained from at least, three independent experiments, * p<0.05, or ** p<0.01.