Figure 1 shows changes in body weight, fat mass and energy intake. Body weight increased throughout the study in all groups, but did not differ from Control after 16 weeks (Fig. 1a). Fat mass was significantly increased in HFD-fed animals compared to both those fed Control and HFr-diets (p < 0.05, Fig. 1b) after 16 weeks. After 8 weeks, accumulated energy intake was significantly higher in NASH-fed rats, compared to both Control- and HFr-fed rats (p < 0.001 and p < 0.05, Fig. 1c) and in HFD-fed rats compared to Control (p < 0.05). This difference remained significant only in NASH-fed compared to Control-fed rats after 16 weeks (p < 0.05).Fig. 1Effects on body weight, fat mass and energy intake of Control-, NASH-, HFD- and HFr-diets. a Body weight increased in all 4 diet groups throughout the study, but did not differ after 16 weeks. b After 8 weeks fat mass was significantly increased in HFD-fed rats compared to Control- and HFr-fed rats. This effect was still present at week 16. c After 8 weeks, accumulated energy intake was significantly higher in NASH-fed rats, compared to both Control- and HFr-fed rats and in HFD-fed rats compared to Control. This remained significant only in NASH-fed compared to Control-fed rats after 16 weeks. Comparisons between groups: *NASH vs. Control; #NASH vs. HFr; †HFD vs. Control. Statistical significance: *p < 0.05; ***p < 0.001; #p < 0.05; ##p < 0.01; †p < 0.05