Inhibition of the BTLA/HVEM interaction was assessed by ELISA (at least two experiments in triplicate). The graph shows percentages of inhibition of the BTLA/HVEM ligation, relative to the negative control (PBS), in the presence of different concentrations (5, 1 and 0.1 mg/mL) of HVEM(23–39) and corresponding scrambled peptide (Ctrl peptide). The gray and dotted back lines correspond to the percentages of inhibition observed with an anti-BTLA blocking antibody (Mean +/-SEM). *: p< 0.05 following non-parametric One-way ANOVA and Dunn’s post-test.