16S rRNA gene sequencing as a clinical screening tool for gut-related conditions has many advantages over traditional culture-based techniques, including ease of sampling, scalability of the test, no need for human interpretation, and the ability to provide additional information about gut health. Most importantly, it can determine the relative abundances of multiple microbial targets, and can therefore be used to detect potential deviations of one or many taxa from that of a healthy cohort. Defining the healthy ranges for gut microbes with known clinical relevance, as done in this study, is likely to bring the analysis of the composition of the gut microbiome one step closer to being part of routine health care analysis [55–57]. Thus, this method of detection for multiple clinically relevant microbial targets is a promising addition to current diagnostic techniques and treatment options.