In a summary, in the present study, we demonstrated that the long-lasting GLP-1 receptor agonist Supraglutide promoted body weight loss in already established obesity in mice. This was associated with improved obesity-related metabolic disorders including hyperglycemia, hyperlipidemia, and hepatic steatosis. Moreover, we found that the beneficial effect of supaglutide on metabolic condition was associated with suppressed food intake and browning remodeling of WAT. The prolonged half-life and metabolically beneficial effect of supaglutide provides an alternative new tool in studying GLP-1 biology and potentially a novel therapeutic target to treat obesity and its associated metabolic disorders.