The 46 targets identified from literature and available clinical tests comprise 15 genera and 31 species. The bioinformatics pipeline for accurate detection of the maximum number of targets is optimized based on the performance metrics Sensitivity, Specificity, Precision and Negative Predictive Value (NPV) as determined with a manually curated amplicon database (described in S1 Doc). The metrics are calculated based on the number of true positives (TP), true negatives (TN), false positives (FP) and false negatives (FN) as follows: specificity = TN / (TN + FP), sensitivity = TP / (TP + FN), precision = TP / (TP + FP) and negative predictive value (NPV) = TN / (TN + FN).