The large scale expression experiments were performed using a previously described 96-well format (Figure 8 and Supplementary file 3) (Box et al., 2011). DNA was digested with DNaseI (Thermo Fisher Scientific, Waltham, USA) and reverse transcription and qPCR reactions were performed as described above using a CFX384 Real-Time System Cycler (BioRad, München, Germany). ACT8 (AT1G49240) and BETA-TUBULIN-4 (AT5G44340) were used as reference genes. Student’s t-tests were calculated with Excel (Microsoft). qRT-PCR primers are listed in Supplementary file 7.