In wheat, TaFlo2-A1 consists of 23 exons that encode 1734 amino acids with four TPR motifs at the positions of 947 to 988, 1032 to 1072, 944 to 1017 and 1028 to 1106 amino acid residues. Furthermore, three mitochondrial CLU domains were also observed at 737–878, 50–162 and 357–401 amino acid residues (Fig. 1a). The rice OsFlo2 gene also consists of 23 exons and encodes 1720 amino acids with three TPR motifs at the positions of 933–966, 975–1008, and 1017–1050 amino acid residues . However, no mitochondrial CLU was reported in rice FLO2 by She et al. . To confirm the absence of mitochondrial CLU in rice FLO2, we BLASTed the rice FLO2 protein (accession: CAE03171) in an NCBI CD search. The results of the rice FLO2 protein (accession: CAE03171) query using the NCBI CD system showed the presence of two mitochondrial CLU domains at the intervals of 52–124 and 721–863 and four TPRs at the intervals of 932–973, 1017–1057, 929–1002 and 1013–1091 amino acid residues (Fig. 1a). Thus, the prediction of wheat and rice FLO2 protein structure using NCBI CD indicates high similarity between their structures.