Calcium hydroxide (CH) is widely used as an intracanal medicament between appointments to increase the number of canals free from bacteria because of its antibacterial, therapeutic, biocompatible, and regenerative properties4,26. However, the remnant CH hinders the penetration of disinfectants and sealers into dentinal tubules and compromises the seal of the canal filling17,22. Therefore, residual CH must be removed before permanent root canal obturation is completed8. In most cases, the residual CH was removed following copious irrigation with sodium hypochlorite (NaOCl) and ethylenediaminetetraacetic acid (EDTA) in combination with the use of master apical file (MAF) or gutta-percha up to working length (WL)24. Different irrigation techniques and devices were used to activate and improve the effectiveness of irrigation solutions7,10,14,25.