In this study we have observed that Tgm9 transposes into unlinked loci following excision from the w4-m mutant allele and that such transpositions could cause functional mutations in over 25% of the mutants. There are 15,166 single copy genes in the soybean genome and Tgm9 should be a suitable tool for generating mutations in most of these genes . However, Tgm9 has preference for certain genomic regions (Fig 2). This will reduce the efficiency of Tgm9 in inducing mutations in such a study for functional analyses of soybean genes. Utilizing Tgm9 to advance soybean genetics and biology will therefore require a large indexed Tgm9-induced mutant population. These mutants can be easily identified by taking the advantage of next generation sequencing platforms [7, 43] and displayed in the SoyBase genome browser (Fig 4). The ability of Tgm9 to precisely excise from mutant loci for reconstituting the wild-type function may eliminate the need of complementation analyses of mutants through genetic transformation for identification of novel genes .