SCF is a paracrine cytokine that plays an essential role in hematopoiesis, maintenance of cell survival, proliferation, and activation, as well as in melanogenesis. Although several studies demonstrated the functions and mechanisms of SCF in the melanogenesis process, those studies mostly focused on pigment production only. This study examined the role of SCF in paracrine factors in fibroblasts and its subsequent influence on melanocytes under a loss of SCF function in fibroblasts. RNA interference (RNAi) is a physiologically mediated mechanism that induces sequence-specific gene degradation. The double-strand RNA undergoes the dicer processes into the siRNA. Binding of siRNA to the target gene causes mRNA degradation directly or arrests the specific protein translation; therefore, the phenomenon is known as gene silencing . Consequently, siRNA is suitable for observing the gene influences, protein functions, and cell developments when the target gene is knocked down and for understanding the role of the target gene. This study used the SCF-targeted siRNA-cooperated transfection method to introduce siRNA into the intracellular environment and inhibit SCF gene expression in fibroblasts. In this study, all the experiments processed the validation and kept the transfection efficiency at a stable inhibitory state.