Splenocyte proliferation is a crucial event reflecting both cellular and humoral immune response because of its considerable sensitivity . Lymphocytes stimulated by Con A in vitro may be used to evaluate T lymphocyte activity associated with cellular immunity, while those stimulated by LPS may be used to evaluate B lymphocyte activity, which participate in the humoral immunity . In this study, CY-induced suppression of Con A-induced T-lymphocyte proliferation and LPS-induced B-lymphocyte proliferation were recovered by AHG. Proliferation assay results suggested that AHG can significantly increase the activation potential of T and B cell proliferation and enhance the immune response in immunosuppression mice.