The indispensability of SLs for a myriad of cellular processes and functions, ranging from structural integrity to signalling events, makes it is unsurprising that the SL biosynthesis is highly conserved in all eukaryotes where it is, alongside its proposed regulators (Holthuis et al. 2006), an essential pathway (Heung et al. 2006; Sutterwala et al. 2007). This has lead the pathway to be considered vital for protozoan pathogenesis and, therefore, a drug target; e.g. SM synthase activity in Plasmodium (Heung et al. 2006). In order to characterise the druggability of protozoan SL biosynthesis, the mammalian pathway, as the most studied system, will be used as the reference model in the following discussions.