More recently, combining different aspects of the above approaches, aqueous angiography was a method that delivered fluorescent tracers into the anterior chamber with external imaging using an experimental microscopic setup  or a clinically FDA-approved angiographic device  (Heidelberg Spectralis HRA+OCT) (Figure 2). Using enucleated human and pig eyes, physiologic pressures were used, and introduction of the tracer into the anterior chamber allowed for simultaneous tracer delivery circumferential around the eye so that segmental changes could be fairly assessed. More importantly, delivery into the anterior chamber allowed outflow and angiographic results to be influenced by the TM.