The effect of RNAi knockdown of GPAT mRNAs on female ability to attract males was analyzed by using Y-tube olfactometer (Fig. 6A). The results showed that the GPAT knockdown significantly reduced female's ability to attract males when tested against EGFP control (GPAT-F1:EGFP = 28:72; GPAT-F2:EGFP = 33:67) (Fig. 6B), similar results were also found in the reciprocal experiments of Y-tube left and right portions (data not shown).