Five genes, W1, W3, W4, Wm, and Wp, regulate pigmentation in flowers and hypocotyls of soybean . The mutant w4-m allele at the W4 locus is characterized by altered pigment accumulation patterns in flowers and hypocotyls . The soybean line with w4-m allele was added to soybean collection and assigned the genetic type collection number T322 . W4 contains the DFR2 gene encoding a functional dihydroflavonol-4-reductase 2 enzyme. Somatic excision of a 20,548-bp CACTA-type transposable element, Tgm9, from Intron 2 of DFR2 causes variegated flowers and hypocotyls with dark brown sectors . Since Tgm9 is located in an intron, both precise and imprecise excisions of the element lead to restoration of the wild-type phenotype. Excision of the element from the germ tissues of the parent plant results in at least some progenies that carry only purple flowers and are termed germinal revertants. The mutation rates in several genetic loci among the revertants with purple flowers were much higher than the rate of spontaneous mutation . Therefore, it was hypothesized that following excision of Tgm9 from DFR2, the element inserts into new genetic loci.