Quantification of specific telomere‐FISH signals in cells and tissues is linearly proportional to telomere length, and differences of telomere length among cells and tissues can be evaluated via quantitative image analysis 18. First, telomere‐specific FISH was applied on whole slides from 30 HCC samples to elucidate the sub‐location and microanatomic distribution of telomere signals in peritumor, tumor margin, and intratumor areas (Figure 2A, B). Telomere signals were higher in peritumor areas (median 25.5; IQR 24.3–26.9) than in intratumor areas (median 11.8; IQR 10.7–13.0) and tumor margins (median 22.6; IQR 20.0–24.4) (Figure 2C).