Mice were housed in constant temperature room with a 12 h dark/light cycle and fed ad libitum on standard mouse chow. In a first study, 35 CalpTG and 35 WT mice have been bred and housed together. At two years, 10 mice from each group have been randomized and sacrificed to perform tissue analysis. The 50 other mice were used to perform a survival study. Some of these mice, in the calpTG group, developed distorted abdomen due to splenic lymphoma and were about to die: these animals have been sacrificed in accordance with ethical guidelines but were not excluded from the survival analyses. All other mice participating in the survival study died spontaneously. Ten CalpTG and 10 WT mice bred in similar conditions have been sacrificed at 2 months (“young” groups). In a second step, 10 CalpTG and 10 WT female mice have been bred and housed in similar conditions for one year to perform hemodynamic studies at 12 months. At last, a specific set of 6 CalpTG and 6 WT female mice has been bred together and sacrificed at 2 years to perform kidney flow cytometry tissue analysis, and four 2-months old CalpTG and WT mice have been used as “young” controls.