Anti-BP180 and anti-BP230 autoantibodies were detected in serum and in blister fluids (BFs) using specific commercially available enzyme-linked immunosorbent assays (ELISAs) following the manufacturer’s instructions (MBL, Nagoya, Japan). Anti-BP180 and anti-BP230 antibodies’ detection was routinely performed on classically used dilution with no further dilution when titers were high. ELISA values were expressed as units per milliliters of serum (U/mL), and the 9 U/mL cut-off value recommended by the manufacturer was used in both anti-BP180 and anti-BP230 ELISAs (29–32).