Supaglutide improves hepatic steatosis of the DIO mice. (A) H&E staining of liver tissues. Scale bar: 100 μm, 400×. (B) Triglyceride content in liver tissue was determined and normalized to the total protein in the samples. (C) Aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were measured in the blood serum of the mice. Significant difference between control and SPG is determined using unpaired Student's t-test. All values are means ± SE (n = 5 for each group). *p < 0.05; ***p < 0.001.