The human ovarian cancer cell lines OVCAR-3, SKOV-3, and OV-90 were purchased from American Type Culture Collection (ATCC, VA, USA). OVCAR-5 cells were obtained from Dr Thomas Hamilton (Fox Chase Cancer Center, PA, USA) and the peritoneal cells, LP-9 were purchased from Coriell Cell Repositories (NJ, USA). COV362 and OAW28 were purchased from the European Collection of Cell culture (ECCC). OV-90, OVCAR-3, SKOV-3 and OVCAR-5 cell lines were grown in RPMI 1640 media (cat no. R8758, Sigma Adrich, St Louis, USA) whilst COV362 and OAW28 were grown in DMEM media (cat no. 10567-022, Gibco, Life Technologies, Mulgrave, Vic, Australia). All cell lines are cultured with 10% fetal bovine serum (Sigma Aldrich) and maintained at 37°C in an environment of 5% CO2. OVCAR-5 cells were made resistant to carboplatin (OVCAR-5 CBPR) following treatment with 8 cycles of carboplatin (CBP, 50 μM, Hospira Australia Pty, Ltd). The OVCAR-5 CBPR cells exhibited an IC50 (273 μM) to carboplatin that was nearly 3-fold higher than that for the parental OVCAR-5 cells (99 μM) (data not shown)