As a result of cell proliferation test, the effectiveness of negative Poisson’s ratio was revealed at 1-day and 3-day culture. However, there was no significant difference in cell proliferation due to diminish of mechanical strength of the scaffold as well as disappear the negative Poisson’s ratio in cultivation at 5-day. The diminishment is presumably caused by viscoelastic behavior of the PLGA in the liquid culture media. Hence, static compressive stimulation is no more effective in 5-day culture period due to mechanical strength reduction and stress relaxation in the scaffold. This might be mechanical stimulation to the scaffold could not be sufficiently transmitted to cells.