It has been established that consumption of raw milk or its products directly is a high risk and that E. coli O157:H7 can cause severe disease and even death . In the U.S., the Centers for Disease Control and Prevention estimates that EHEC O157:H7 causes approximately 73,000 illnesses, 2000 hospitalizations, and 50-60 deaths each year . In Libya, it is common and widely practiced to manufacture dairy products from raw milk. Isolation of E. coli O157 from milk and dairy products examined in this study could be associated with human diseases following their consumption. However, it is not possible to link our findings with any of the food poisoning cases that were occurred in Libya because of the lack of proper documentation of such cases.