Macrophage infiltration and collagen deposition. A significant increase in macrophage infiltration was seen in NASH-fed rats, compared to Control- and HFD.fed rats (a) and collagen deposition in NASH-fed rats trended towards being increased, although only significantly when compared to HFD-rats (b). Representative CD68- (c) and Picro Weigert-stains (d) of liver from NASH-fed rat. Statistical significance: **p < 0.01; ***p < 0.001. Results in a and c are shown as mean ± SEM