RNA was harvested from 10 × 106 MDA-MB-231 cells that were mock treated or treated with 100 ng/ml cmvIL-10 or hIL-10 for 5 h using the RNeasy Midi Kit and RNAse-Free DNase set (Qiagen, Valencia, CA). From the isolated RNA, cDNA was prepared using the RT2 First Strand Kit (SA Biosciences, Frederick, MD) and subsequently loaded into a 96-well breast cancer metastasis profiler PCR array (PAHS-028ZD) with system RT2 SYBR Green Mastermix (SA Biosciences). The plates were run using the CFX96 Real-Time system cycler (BioRad, Hercules, CA) with the following amplification program: 95 °C for 10 min, 95 °C for 15 min with a slow ramp rate for 1.0 c/s and 60 °C for 1 min. The data from three biological replicates for each treatment was analyzed by the ΔΔCT method according to manufacturer’s instructions using the RT2 profiler PCR array data analysis program located on the SABiosciences web portal and is reported as fold change relative to control.