While recent genomic work has shown that some spider species may possess more than two MaSp loci, with some paralogs exhibiting different expression patterns across different silk glands , further verification of multiple MaSp loci across the Araneoid clade awaits. Since our objective here was to identify whether shifts in amino acid compositions can be attributable to shifts in MaSp1: MaSp2 expression and not verify or refute the multiple loci hypothesis, we used the abovementioned procedures to ascertain the across treatments expression patterns of just two MaSp1 and MaSp2 paralogs.