Interleukin (IL)-1β and IL-8 levels in the HDLP group obviously rose (P < 0.05) relative to those of the CONT group at 0 h after ETEC challenge. At 24 h after ETEC challenge, the IL-1β, IL-8, IL-6, TLR4, and MyD88 levels in the ETEC group were significantly higher (P < 0.05) than those of the CONT, LDLP, and MDLP groups. Meanwhile, the IL-8 contents did not significantly change (P > 0.05) between the ETEC and HDLP groups. At 144 h, the IL-1β expression in the ETEC group was higher (P < 0.05) than those of the other groups (Figure 6).