PCR based screening for polyketide synthases (PKS I & PKS II), nonribosomal peptide synthetases (NRPS), aminodeoxyisochorismate synthase (phzE), and cytochrome P450 hydroxylase (CYP) genes was performed using the degenerate primers reported previously (Izumikawa 2003; Wawrik et al. 2005; Ayuso-Sacido and Genilloud 2005; Lee et al. 2006; Schneemann et al. 2011). The composition of the reaction mixture for all PCR amplifications was: 0.4 µL template, 5 µL 2× EmeraldAmp GT PCR Master Mix (Takara Bio Inc., Japan), and 0.5 µL each of the primers (10×).