In total, 43 genomes representing Lp1 (Philadelphia, Paris, Corby, Lorraine, LPE509, and Alcoy) and Lp serogroups Lp2-17 (including Lp12 strain 570-CO-H), L. pneumophila subspecies (Thunder Bay and HL06041035), and 17 other species L. longbeachae, L. bozemanii (serogroups 1 and 2), L. dumoffii, L. micdadei, L. birminghamensis, L. brunensis, L. cherii, L. cincinnatiensis, L. erythra, L. jamestowniensis, L. jordanis, L. maceachernii, L. nautarum, L. rowbothamii, L. tucsonensis, L. wadsworthii, and L. anisa were used for comparative genomics analyses of which 33 were sequenced in this study (Supplementary Table 1). Genomic DNA from the 33 serogroups and species was sequenced with both 454 and Illumina (76 bp single end) technology. Burnstein et al.19 recently genome sequenced different strains from the current study including L. anisa, L. bozemanii, L. brunensis, L. cherii, L. cincinnatiensis, L. dumoffii, L. erythra, L. jamestowniensis, L. jordanis, L. maceachernii, L. micdadei, and L. nautarum.