Figure S3. Relative telomere length detected by qPCR. (A) Shortened RTL was confirmed in tumor compared with adjacent non‐tumor tissues (n = 24). ***p < 0.001. (B) Shortened RTL was validated in CAFs compared with that in NTFs (n = 10). **p < 0.01. CAFs and NTFs were isolated using microbeads as described in the Materials and methods. (C) No significant difference was found in PTILs and TILs (n = 10). PTILs and TILs were isolated using microbeads as described in the Materials and methods. (D) The relative telomere length of tumor cells correlates significantly with the relative TERT mRNA level (n = 64; r = 0.806, p < 0.0001). (E) Representative images showing telomere intensity in paired tumor cells and peritumor liver cells. Case #29: fewer telomere signals in tumor cells than in paired peritumor cells; case #41: stronger telomere signals in tumor cells than in peritumor liver cells. (F) Representative images showing telomere intensity in paired NTFs and CAFs. Case #32: fewer telomere signals in CAFs than in NTFs; case #44: stronger telomere signals in CAFs than in NTFs. Short white arrows indicate NTFs and long white arrows CAFs. Original magnification ×40.