The production of Wa1F1-KT by the strain W. anomalus 1F1 was analyzed over time. The activity of the concentrated culture supernatant obtained at different time periods was determined against the reference C. lusitaniae strain on solid medium and quantified by Arbitrary Units/mL (1 Arbitrary Unit (AU) is defined as the amount of KT producing an inhibition zone of 1 mm2). As shown in Figure 1, the results indicate that the activity of the Wa1F1-KT sample obtained after 24 h of incubation was low (559 AU/mL), although it increased after 48 h of incubation (1815 AU/mL), and reached a maximum after 72 h (2326 AU/mL). The killing activity decreased steeply thereafter.