All Arabidopsis thaliana accessions used in this study as well as flm-3 (Salk_141971; Col-0) and Nd-1 (N1636) were provided by the Nottingham Arabidopsis Stock Centre (NASC; Nottingham, UK). flc-3 and FRISF-2 FLC (Michaels and Amasino, 1999) were a gift from Franziska Turck and George Coupland (Max-Planck Institute of Plant Breeding Research, Cologne, Germany). pFLM::gFLM (pDP34), pFLM::FLM-ß (pDP96), and pFLM::FLM-δ (pDP97) were previously reported (Posé et al., 2013). Primers to screen the accessions for the FLMLINE insertion were used as previously described (Lutz et al., 2015).