Two biopsied specimens of each patient were taken: one specimen from antrum (lesser curvature side) and another from low body (greater curvature side). H. pylori isolates were cultured from the biopsies specimen and identified by biochemical reactions . H. pylori were diagnosed with positive reaction in catalase, urease, and oxidase tests. The bacterial isolates were routinely cultured on Brucella agar plates (Becton Dickinson, Franklin Lakes, NJ) with appropriate antimicrobial agents as described previously .