(iii) The average differentiation between D. rotundata and D. praehensilis was higher than between D. rotundata and D. abyssinica, (FST = 0.21 and 0.16, respectively, p-value <0.001). Using a 1% threshold (FST > 0.73 and 0.84 for D. rotundata with D. praehensilis and D. abyssinica respectively), 422 contigs were identified with extremely high FST values with one or the other wild species. Among them, 12 showed extreme values with the two wild species simultaneously (Fig. 2-c).