Note that the AFM study (van Pee et. al, Nano Letters, 2016, Figure 5) describes the transformation of membrane-attached rings into pores as irreversible, not the membrane attachment of the rings. Rings of this PLY mutant in the early prepore state detach from the membrane easily, but only before they (irreversibly) insert into the membrane to form the pore. This is now discussed in on paragraph two of the Discussion section.