We evaluated whether SCF affected the fibroblasts and the mRNA paracrine factor levels from fibroblasts. To determine how differently the paracrine factors reacted due to SCF silencing, the controlled gene expressions were compared to the gene expression with SCF knockdown. In Figure 2, the data showed a significant increase in HGF, NRG, and CRH genes when SCF was stably silenced 48 h post transfection. The downregulated mRNA levels with DKK-1 and ET-1 were affected because of SCF knockdown in fibroblast cells. ET-1 was the most severely affected gene, while the paracrine growth factor HGF was the least affected of all the genes. SCF gene alteration affected the fibroblast paracrine system and its knockdown increased or decreased all the specific paracrine gene expression levels.