In B. mori PGs, LDs rapidly accumulated before adult emergence. These LDs contain various TAGs which provide the precursor fatty acids and are released for sex pheromone production in response to PBAN. The LDs fluctuate in both size and number in accordance with the fluctuation of PBAN release and bombykol amount18. These fluctuations in LD and bombykol amount could be prevented by decapitation and preceded by PBAN injection. The amount of TAGs also significantly decreased after PBAN application. These results clearly demonstrated that PBAN stimulated the lipolysis by activating lipases which hydrolyze TAGs for the release of bombykol precursor. LSD-1 was found to play an important role in TAG lipolysis associated with bombykol production in B. mori. PBAN-mediated calcium influx led to the activation of a series of components, including CaMKII, which activates LSD-1 phosophorylation. The activated LSD-1 then resulted in activation of the associated lipases. RNA Seq studies found that seven lipases were highly expressed in B. mori PGs at key stages of sex pheromone biosynthesis. RNAi-mediated knockdown confirmed that four of seven lipases are involved in TAG lipolysis11.