Yeast strains arl1Δ (MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15ura3Δ0/ura3Δ0, arl1Δ::KanMX/arl1Δ::KanMX) and ypt6Δ (MATa/α his3Δ1/his3Δ1 leu2Δ0/leu2Δ0 LYS2/lys2Δ0 met15Δ0/MET15ura3Δ0/ura3Δ0, ypt6Δ::KanMX/ypt6Δ::KanMX) were used in the high copy suppressor screen for rapamycin sensitivity at 37°. Both strains were obtained from the homozygous diploid deletion collection developed by the Saccharomyces Genome Deletion Project (Winzeler et al. 1999); the parental strain is BY4743. Strains YSA003 (pho8::pho8Δ60 arl1Δ::HIS3) and YSA004 (pho8::pho8Δ60 ypt6Δ::HIS3) in the BY4742 background (MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0) were constructed previously (Yang and Rosenwald 2016). High copy plasmids containing a single specific yeast ORF were either isolated from the screened library (COG4 and SNX4) or were obtained from the yeast ORF collection (SLT2, ATG5, PEP3, IVY1, and TAX4) (Gelperin et al. 2005). The pRS316-GFP-Atg8 plasmid was a gift from Daniel Klionsky (University of Michigan) (Suzuki et al. 2001). The plasmids used in this study are listed in Supplemental Material, Table S1.