In this study, to gain insight into the role of the capsid protein VP1 in the life cycle of CAV, we have investigated the physical interactions of CAV VP1 with the viral DNA. A recombinant E. coli expression system was used to express the recombinant VP1 of CAV following our previous study . The intracellular localization of the CAV VP1 was observed in MDCC-MSB1 cells or CHO-K1 cells using fluorescent green protein in the nucleoplasmic compartment. The DNA-binding activity of VP1 was also systemically examined. To the best of our knowledge, this is the first report to verify the DNA binding activity of the CAV capsid protein, VP1.