The total BEV read counts (percentages indicate BEV reads per total reads of the first and second deep sequencing) of the Kagoshima sample were 1202 reads (0.05%), and an approximately 7400 nt contig was obtained from the integrated result with a 24.24 average sequence read depth (maximum read depth was 46). The complete genome was determined using 5′ and 3′ end RACE methods. Because amino acid sequences of VP1 were not similar to those of other enteroviruses by homology analysis as described below, the VP1 genome sequence was confirmed by directly sequencing the PCR product. As a result, sequences obtained from direct sequencing agreed with the results of deep sequencing. The genome length of BEV from the Kagoshima sample was 7414 nt, excluding the poly (A) tail. We named this BEV as BEV AN12/Bos taurus/JPN/2014 (BEV-AN12).