Immunofluorescence: Cells were plated onto sterilised glass coverslips (VWR 19 mm) in 35 mm plates 24 h before transfection at a density of 1.2 × 105 cells in a total volume of 2 mL media. Forty-eight hours following transfection, cells were fixed to coverslips in 100% methanol for 5 mins at −20 °C. Cells were then washed with PBS and blocked with 1% donkey serum in PBS/0.05% Tween for 30 min at room temperature. Cells were placed in a humidified chamber, incubated with primary antibody for 1 h, washed three times (5 min each) in PBS, incubated for 1 h with secondary antibody, and washed three times (5 min each) in PBS. Nuclear staining was performed by incubation with 33.3 μM Hoechst (Life Technologies) for 2 min. Coverslips were then washed in dH2O and mounted onto VWR SuperFrost slides using mounting medium (H-1000; Vector labs, Peterborough, UK) and sealed with nail varnish. Cell images were acquired using a DeltaVision microscope. Images were deconvolved and analysed using OMERO client software (5.2.7, Open Microscopy Environment, Dundee, UK) .