Following fixation in 4% PFA for 48 hours the specimens were washed in deionized water for 10 mins. The specimens were then dehydrated in ascending ethanol washes (50%, 70, 90%, 100%) for 10 mins each. Following gold coating, the specimens were placed on carbon coated alumminin stubs before being imaged (FEI Quanta 200 F, n = 3).