Investigation and deciphering of the functions of each specific SL species remains challenging. This is due to the complexity in SL metabolic interconnections, their varied biophysical properties (neutral or charged), chain length variation, the hydrophobic nature of the involved enzymes and the presence of multiple pathways that can operate in parallel (Hannun & Obeid, 2008). The interaction with other cellular metabolic pathways (e.g. glycerolipid metabolism) introduces another layer of complexity.