The metabolic reconstructed model of Escherichia coli iJO1366 (Orth et al., 2011) was used for analyzing gene overexpression strategies in lycopene precursor overproduction. And the reconstructed model of Synechocystis sp. PCC 6803 iJN678 (Nogales et al., 2012) was used for the analysis of fatty acyl-ACP overproduction.