We showed that metformin infusion at a dose of 200 mg/kg/day reduced Glut2 abundance in hepatocytes, which demonstrated effective delivery and the targeting of liver function by the drug. Glut2 is a bidirectional glucose transporter present in liver, kidney, intestine, and pancreas, which facilitates glucose transit from the interstitium to the cell and vice versa. Studies have shown that Glut2 is overexpressed under diabetic conditions due to the increased glycolysis associated with the enhanced glucose transport (Zhang et al., 2013). Over-expression of Glut2 in hepatic tissue was found in high-fat and fructose-induced type-2 diabetic animals. Anti-diabetic treatment reduced Glut2 expression in high-fat and fructose-induced type-2 rats (Narasimhan et al., 2015).