We, next, examined this interplay between hyperglycemia and TNF-α in a coculture experiment, using BeWo trophoblast-like cells and freshly isolated control neutrophils (Figure 4). Here, we observed that HG conditions (25 mM) promoted neutrophil activation, identified by staining for citH3 (Figures 4A,B), as well as enhanced NET formation (Figures 4A,C) identified by co-staining of extruded nuclear material for NE and citH3. Both neutrophil priming and NET formation were reduced by addition of the TNF-α antagonist, infliximab (Figures 4A–C). In this context, it is of interest that infliximab treatment not only reduced the number of NETs but also had a noticeable influence on their size and dimensions (Figure 4A).