Severe gut disease can damage host health. However, in giant panda, this kind of disease must be prevented and treated. The relationship between probiotics and improved gut health has received considerable scientific interest for more than a century. To protect animal guts, scientists have shown that probiotics, such as Lactobacillus, Bifidobacterium, and Bacillus, prevent gut bacterial disease (Barba-Vidal et al., 2017; Nishida et al., 2017; Wu et al., 2017). In the present study, L. plantarum G83 was isolated from the feces of healthy giant panda and confirmed beneficial in vitro. This current work aimed to evaluate the protective effect of L. plantarum G83 in mice challenged with ETEC. To accomplish this objective, we assessed mice for their weights, as well as chemical and biological intestinal barriers.