Thromboelastography (TEG®) is a viscoelastic technique for measuring the clotting properties of WB . TEG of WB and PPP was performed. TEG was not performed with purified fibrinogen because the coagulation activator in the TEG is CaCl2 and fibrinogen is only activated by thrombin, not calcium. Figure 7 shows a typical TEG trace from a control WB with and without added LPS, overlaid with lines that explain the parameters extracted by the instrument and the values for those traces. The statistics are given in table 1. Figure 7.TEG overlay from a control whole blood sample with and without added LPS. R, reaction time, first measurable clot formation; K, achievement of clot firmness; angle, kinetics of clot development; MA, maximum clot strength; MRTG, maximum rate of thrombus generation; TMRTG, time to maximum rate of thrombus generation; TTG, final clot strength. (Online version in colour.) Table 1.Demographics of blood from healthy individuals with and without added LPS. Medians, standard deviation and p-values (values lower than 0.05 are indicated in italic) obtained using the Mann–Whitney U-test are shown for iron profiles, and TEG of whole blood and plasma. R, reaction time, first measurable clot formation; K, achievement of clot firmness; angle, kinetics of clot development; MA, maximum clot strength; MRTG, maximum rate of thrombus generation; TMRTG, time to maximum rate of thrombus generation; TTG, final clot strength.variableshealthy individuals (n=30)healthy individuals with added LPS (n=30)p-valueage (years)29.5 (±13.81)gender male15 (50%) female15 (50%)iron profiles iron µM16.9 (±6.18) transferrin g l−12.75 (±0.46) % saturation25.0 (±10.94) serum ferritin ng ml−142.5 (±92.47)fibrin fibre thicknessn = 1450n = 1330 fibre thickness (nm)103 (±40)86 (±82)<0.0001TEG®TEG of whole blood—recalcified with CaCl2 with added O111:B4 LPS (10 min)MRTG (dyn cm−2 s−1)2.61 (±1.13)2.89 (±0.90)0.33TMRTG (min)13.9 (±3.53)9.6 (±3.01)<0.0001TTG (dyn cm−2)615.0 (±179.55)527.9 (±146.65)0.049R (min)8.0 (±1.64)6.2 (±1.77)<0.0001K (min)4.9 (±2.63)4.2 (±1.23)0.07angle (°)49.8 (±5.27)56.2 (±7.02)0.0066MA (mm)55.0 (±8.07)51.3 (±6.90)0.092TEG of platelet-poor plasma—recalcified with CaCl2 with added O111:B4 LPS (10 min)MRTG (dyn cm−2 s−1)3.6 (±4.35)4.2 (±2.13)0.36TMRTG (min)10.6 (±3.22)9.3 (±3.68)0.50TTG (dyn cm−2)203.9 (±137.51)211.6 (±103.67)0.70R (min)8.2 (±2.64)7.1 (±2.70)0.026K (min)4.4 (±3.51)3.8 (±2.42)0.18angle (°)63.2 (±2.70)54.4 (±10.67)0.23MA (mm)28.4 (±8.34)30.1 (±9.27)0.196TEG of platelet-poor plasma—recalcified with CaCl2 with added O111:B4 LPS (30 s)n = 5n = 5MRTG (dyn cm−2 s−1)5.94 (±1.8)8.2 (±2)0.166TMRTG (min)11.58 (±1.2)9 (±1.3)0.0159TTG (dyn cm−2)244.4 (±69.9)290.2 (±66.5)>0.99R (min)9.8 (±1.2)7 (±1.5)0.031K (min)2.8 (±1.6)2 (±0.9)0.119angle (°)63.6 (±6.5)68.8 (±8.2)0.095MA (mm)32.8 (±6.5)36.7 (±5.5)>0.99TEG of platelet-poor plasma—recalcified with CaCl2 with added O26:B6 LPS (30 s)n = 5n = 5MRTG (dyn cm−2 s−1)6.3 (±2.6)6.2 (±3.6)0.70TMRTG (min)11.6 (±2.1)8.9 (±1.5)0.15TTG (dyn cm−2)276.7 (±43.1)230.8 (±202.2)0.69R (min)9.8 (±1.5)6.4 (±1.4)0.05K (min)2.1 (±0.5)2.1 (±1.1)0.88angle (°)64.4 (±4.3)70.8 (±10.4)>0.99MA (mm)35.5 (±3.3)31.5 (±13)0.60