10.7554/eLife.23644.017Figure 6—figure supplement 1.CryoET map of the PLY prepore with rigid-body fitted x-ray structures of the water-soluble toxin that forms rows in the 3D crystals.PLY monomers of 5AOD (green) are straight and protrude by 10 Å at the top of the map. Monomers of 5CR6 (red) are bent at the hinge between domain 2 and 4 (blue arrow) and match the map volume closely, indicating that this structure represents the pre-pore form of PLY.DOI: http://dx.doi.org/10.7554/eLife.23644.017