Sox2 was expressed in the spheres of glioblastoma and gliosarcoma, and also played an important role in the epithelial mesenchymal transition of glioma stem cells . Our study found that Sox2 was expressed in nuclei and cytoplasm. Some studies found that Sox2 high expression might be associated with invasion of gastric cancer and poor survival outcomes [20, 22, 39]. Loss of expression of Sox2 indicated a worse prognosis . However, we found that Sox2 high expression was a favorable prognostic factor.