Bone marrow cells from tibia, femur and humerus of donor mice (WT or CalpTG) were obtained after bones were flushed with 1 ml of PBS. Cells were passed through 70 µm sieve, treated with ACK and finally incubated for 7 to 10 days at 37 °C in a medium containing HAM’s F12 250 ml, DMEM 250 ml, decomplemented fetal calf serum 10%, glutamine 200 mmol.l−1 and mouse recombinant M-CSF 10 ng.ml−1 (ML 416, R&D systems) to obtain adherent BMDM. BMDM were plated in 96 wells (5.105.ml−1) and primed with 10 ng.ml−1 Lipopolysaccharides (LPS) from Escherichia coli 055:B5 (Sigma-Aldrich) during 3 hours and treated with inflammasome activators for 1 to 6 hours: MSU 300 µg.ml−1 (Invivogen), silica particles 100 µg.ml−1 (Invivogen), ATP 5 mmol.l−1 (Sigma-Aldrich) and Nigericin 5 µmol.l−1 (Invivogen). Il-1α and Il-1β excretions were measured in cell supernatant by ELISA (MLA00, MLB00C, R&D systems). Cells mRNA has been collected using the RNeasy kit (Qiagen) according to manufacturer’s instructions.