SDS and analytical grade ethanol were obtained from Merck (Darmstadt, Germany). Disodium hydrogen phosphate dihydrate (Na2HPO4 × 2H2O), potassium dihydrogenphosphate (KH2PO4), sodium chloride, Nonidet P-40 substitute (NP-40), 1-hydroxybenzotriazole monohydrate 97% (HOBt), 50% sodium hydroxide (NaOH), and super-DHB were obtained from Sigma-Aldrich (Steinheim, Germany). HPLC-grade acetonitrile (ACN) was purchased from Biosolve (Valkenswaard, the Netherlands), and 1-ethyl-3-(3-(dimethylamino)propyl)carbodiimide (EDC) hydrochloride was obtained from Fluorochem (Hadfield, UK). Peptide-N-glycosidase F (PNGase F) was obtained from Roche Diagnostics (Mannheim, Germany) and ultrapure water (MQ) was generated from a Purelab Ultra system (Veolia Water Technologies, Ede, the Netherlands), which was maintained at 18.2 MΩ at 25°C.