Recommended maximum storage T/F cycles and maximum storage time at a given temperature were set (for each method individually) as the most extreme data point that remained within the control range. NISTmAb RM 8671 was shown to be quite stable with respect to T/F according to UV, SEC, CE-SDS, and CZE. In all cases the material produced a measurement result within the control range for up to 5 T/F cycles. According to Fig. 9, however, the T/F conditions appeared to show particle concentrations near or outside of the defined control regions, therefore it is recommended that T/F not be performed for samples intended for flow imaging analysis.