To assess whether variants at rs7163757 might cause changes in the expression of nearby genes, two reporter constructs were generated. A 1.3-kb fragment of the genomic region flanking the SNP was amplified by PCR from a heterozygous donor by using Phusion High Fidelity DNA Polymerase (Thermo Scientific, Paisley, UK). The PCR product was subsequently cloned into CR™8/GW/TOPO (Thermofisher, Paisley, UK) according to the manufacturer's instructions. Plasmid DNA from clones was purified using a GenElute Plasmid Miniprep Kit (Sigma, Dorset, UK) and sent for sequencing to identify clones containing one of each allele. DNA fragments were then shuttled into the minimal promoter (DNA sequence: TAG AGG GTA TAT AAT GGA AGC TCG ACT TCC AG, containing a TATA box promoter element)-driven luciferase vector GL4.23-GW vector (76) using the Gateway LR Clonase II Enzyme Mix (Invitrogen, Paisley, UK). pGL4.23-GW is modified from pGL4.32 (Promega) with Gateway technology (Thermofisher) and has previously been used successfully for the analysis of enhancer activity (20).