B16 melanoma cells were used as the target cells to evaluate the specific cytotoxicity of activated-CTLs. The results shown in Figure 3 indicated that the cytotoxicity of CTLs in AHG groups ranged from 0.5 to 25 μg/mL, significantly higher than that in NC group. AHG at 5 μg/mL possessed the highest cytotoxicity (p < 0.01). Thus, AHG could enhance the cytotoxic of CTL stimulated by DCs on melanoma cells in different degree.