DuoSet ELISA kits (R&D Systems) were used to quantify uPAR, PAI-1, and MMP-3. For uPAR and PAI-1 measurement, MDA cells were seeded in triplicate in 96-well plate at 5.0 × 104 cell/ml density with complete L-15 media and treated with 10 ng/ml of either cmvIL-10 or hIL-10 for the indicated times and supernatants were collected daily. The ELISA was carried out on supernatants according to manufacturer’s instructions using and following the addition of substrate and stop solution, absorbance of the plate was measured at 450 nm using a Dynex Opsys MR microplate reader. Sample concentrations were interpolated from a standard curve using linear regression analysis. For cell-associated MMP-3, MDA cells were seeded in 96-well plates and treated with cmvIL-10 as above. Cells were treated with cell lysis buffer (150 mM NaCl, 20 mM HEPES, 0.5% Triton-X-100, 1.0 mM NaOV4, 1.0 mM EDTA, 0.1% NaN3) supplemented with 1× protease inhibitors (Calbiochem, EMD Chemicals, San Diego CA) and were collected daily for the indicated time points. The lysates were evaluated for MMP-3 according to the manufacturer’s instructions (R&D Systems).