Based on the folding/unfolding rates, we can also assess the energetics of the individual conformational transitions observed here (Figure 3 and Supplementary Table S4). The folding transitions to the 2-tetrad basket and hybrid 2 conformations are unfavorable under the current experimental conditions, while the chair is slightly favored. Our results reveal that the hybrid 1 conformation is the most stable and dominant conformation at the folding equilibrium, which stability \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}$(\Delta{G_U}\sim 3\,{k_B}T)$\end{document} is in reasonable agreement with the previously reported values from ensemble (50,51) and single-molecule (25) experiments under similar buffer conditions.