Given that hypoxia induces changes to KDM2 proteins, we next determined if these were HIF dependent. First, we analysed KDM2A protein levels in response to hypoxia in the presence or absence of HIF subunits. Western blot analysis revealed that KDM2A protein levels were induced in hypoxia in a HIF-1α-, HIF-1β-dependent manner, while HIF-2α did not contribute to these effects (Figure 3A). Interestingly, KDM2B protein levels are also HIF-1α dependent, as HIF-1α depletion resulted in higher KDM2B protein levels both in normoxia and especially in hypoxia in U2OS cells (Figure 3B). This data was also observed when additional siRNA oligonucleotides were used in HeLa cells (Figure S3A).