Overall, the temperature did significantly affect mortality (F(1,98) = 9.90, P = 0.002). No adult mosquitoes emerged at the two lowest temperatures (0 °C, 5 °C; Table 1). Larvae survived for at most three days at 0 °C. Larvae reared at 5 °C almost completely died and eventually, only one survived in larval stage > 100 days. In all other temperature treatments, the mean mortality was ≤ 50% (Table 1). Cumulative female survival was calculated and upper and lower estimated developmental thresholds were provided (Fig. 2). The best fit of temperature-dependent cumulative female survival was Briere et al.  eq. 1-model (Additional file 1: Figure S2) with a = 6 × 10−6 and m = 2.0. The model selection based on AIC weights is presented in Additional file 1: Figure S2. Optimum temperature was 26 °C and lower and upper thermal limits were 7 °C and 31 °C, respectively (Table 1, Additional file 1: Figure S1).Table 1Temperature effects on mortality of the Asian bush mosquito. Mean mortality for each temperature treatmentTemperature (°C)Mean mortality (%)SD (%)0100.00.05a99.51.11016.05.51238.514.21418.04.81515.07.91719.09.61929.512.42011.36.52348.527.62513.88.4266.05.22741.531.12812.57.72970.522.23187.56.4aFor 5 °C, the mortality was calculated on an experimental day 113. One individual survived to this day and was considered alive in the analysis. For all other temperatures, mortality reflects cumulative larval and pupal mortalityAbbreviation: SD standard deviationFig. 2Thermal reaction norm and limits for the development and survival of the Asian bush mosquito. Cumulative female survival per day assessed over a range of 14 constant temperatures is shown. The solid black line represents the fitted curve calculated with Briere et al.  eq. 1. The function has the following form: cumulative female survival y = aT(T-Tmin)(Tmax-T)1/m with a, m: empirical constants (determined during model selection; Additional file 1: Figure S2), Abbreviations: T, temperature; Tmin, minimum temperature for cumulative female survival; Tmax, maximum temperature for cumulative female survival; Topt, optimum temperature for cumulative female survival calculated with the first derivative equation dy/dT = 0