Two month-old CBA/Ca female mice were purchased from Harlan Interfauna Ibérica S.A. and housed under standard conditions. Mice were randomly divided into two experimental groups (n = 10 each) that were fed the A04/A04C/R04 diet (Panlab/SAFE) containing standard folate levels (normal folate 2 mg/kg; NF) or a FD diet (folic acid ≤0.1–0.2 mg/kg, Harlan Tecklad TD.95247) ad libitum for 8 months. Weight gain was measured weekly for both dietary regimes. All experiments were approved by the CSIC Bioethics Committee and carried out in full accordance with the guidelines of the European Community (2010/63/EU) and the Spanish regulations (RD 53/2013).