Complete ORF transcripts of CsTegu20.6 were amplified from specific stages of C. sinensis, including adult worms, metacercariae and eggs, using the previously described primers. PCR was performed using a thermal cycling profile of 95 °C for 1 min, 30 cycles at 94 °C for 30 s, 55 °C for 30 s and 72 °C for 1 min followed by a 72 °C extension for 10 min. The analysis of transcripts obtained from each cDNA library  was performed by 1% agarose gel electrophoresis and ethidium bromide staining.