Protozoal triosephosphate isomerases (LmexTIM and TcTIM) are expected to be targeted by Copaifera diterpenoids. The average docking energy with LmexTIM (−90.7 kJ/mol) was much better than either the normal substrate (dihydroxyacetone phosphate, Edock = −52.4 kJ/mol) or the co-crystallized ligand, phosphoglycolohydroxamic acid (Edock = −61.1 kJ/mol). Likewise, docking energies with TcTIM (average −88.2 kJ/mol) were better than the dihydroxyacetone phosphate (Edock = −59.7 kJ/mol) and comparable to the TcTIM inhibitor, 3-(2-benzothiazolylthio)-1-propanesulfonic acid (Edock = −85.5 kJ/mol).