In China, most donations were routinely subjected to screening for HBsAg by ELISA twice. The remaining few blood centers screen HBV infection by ELISA(×1) plus NAT. However, due to the transient nature of the HBsAg detectable period during the HBV infection process, and also due to the lack of routine screening testing for anti-HBc antibodies, the residual risk of HBV infection has remained high in China [8, 13, 16]. In order to develop an evidence-based, efficient and safe blood donor screening strategy and/or policies to decrease the RR of HBV infections, it is essential to have epidemiological information regarding the prevalence, incidence and RR of HBV infections and the associated demographic characteristics of both high-risk and low-risk populations of voluntary blood donors. The demographic characteristics of volunteer blood donors in different blood centers in China were quite different. For example, the percent of the first time donors among the six blood centers ranged from 32.43% to 67.11% of all donations. The proportion of young donors 18 to 25 years old was 43.22% of all donations at the Anhui Blood Center, but only 8.23% at the Changzhi Blood Center. Meanwhile, the proportion of middle aged donors 35 to 55 years old was 28.33% of all donations at the Anhui Blood Center, but was 66.93% at the Changzhi Blood Center. There were also some differences in the occupations and education levels at the different blood centers. For instance, 37.39% of donors were farmers, 18.00% were workers and 4.31% were students at the Changzhi Blood Center, while these proportions were 3.07%, 2.9% and 17.53%, respectively, at the Fujian Blood Center. 91.94% of donors had either received an Associate’s degree, a high school diploma or below at the Changzhi Blood Center, while this figure was 56.68% at the Dalian Blood Center. However, some similarities were noted among different blood centers regarding the gender of the donors; namely male donors comprised the majority of donors (with a mean of 61%) at all of the blood centers. In addition, more than half of all donors at all of the blood centers were under 35 years old in this study except for those at the Changzhi Blood Center (see Table 3).