P.K. conceived and performed the research and wrote the manuscript with contributions from P.E.B, Z.M, J.V, Y.Y, R.A, D.E, J.B, P.N, J.D and W.E. The BioNano Irys®;System genome mapping experiments were led and designed by J.D and performed and analysed by Z.M, J.V and Y.Y. P.E.B and Y.Y did the bioinformatics analysis and helped with the figure preparations. All authors read the manuscript and approved the content.