Confocal live imaging of Drosophila (A) muscles and (B) tracheal system labeled with NRB-AF12 (green) and ER-Tr red (red). Both fluorescent dyes, added to the dissected larva in HL3 physiological solution, show an overlapping pattern of distribution as highlight by the yellow signal of the merged images (*: muscle; white arrowhead: trachea; N: nucleus). Magnification 60x; scale bar 10 μm.