For FITC-gelatin degradation analysis, coverslips were coated with 50μg/ml poly-L-lysine (Sigma-Aldrich #P8920) in PBS, and then incubated with 0.5% glutaraldehyde (Sigma-Aldrich #G6257) in PBS. The slips were coated with 1:40 fluorescent 488 gelatin (‘FITC-gelatin’) (Life Technologies #G13186) diluted with 0.2% unlabeled gelatin (Sigma-Aldrich) in PBS for 30 min. at 37°C. Cells were plated for 24 hours, fixed and stained as above. For quantitation of matrix degradation, nine random fields (20x objective) were imaged from each sample (n = 3). ImageJ software was used to threshold the cell area as well as areas of matrix degradation (black areas in FITC-gelatin) and to calculate a ratio of matrix degradation to cell area. Statistical analyses were performed using a one-way ANOVA with a Tukey correction.