The DNA fragmentation observed in the TUNEL assay confirmed apoptotic cell death in cells treated with the S. artemisiae MCCB248 extract. Further, a time-dependent increase in Annexin V positive cells implied more cells had undergone a flip-flop of PS to the outer leaflet of the plasma membrane, another indicator of apoptosis. These results demonstrated that S. artemisiae MCCB 248 extract induced apoptotic cell death in NCI-H460 lung cancer cells.