Metformin is well-known to modulate cellular metabolism in different tissues. Glut2 is a glucose transporter that mediates diffusive glucose transport through the plasma membrane in a variety of tissues (Thorens, 2015). To confirm that the infusion of metformin reached its targets and had functional effects, we assessed Glut2 expression in liver tissue collected after vehicle and metformin treatment. Immunohistochemistry assays revealed that metformin dramatically decreased the abundance of Glut2 in hepatocytes (Figure 3B). Therefore, 200 mg/kg/day intravenous metformin infusion had functional consequences in this rat model, altering Glut2 expression and presumably liver metabolic function.