Male Kunming mice weighing 25–30 g were purchased from Qingdao Institute for Drug Control (Qingdao, China, SCXK2009007), and maintained under controlled conditions (temperature: 25 ± 2 °C, humidity: 50 ± 5%, 12 h dark-light cycle). The animals were acclimated for 7 days with free access to standard diets and sterile water. All of the animal experiments adhered to strict compliance according to Animal Ethics Committee of School of Medicine and Pharmacy, Ocean University of China for the use and care of animals.