Haemoplasmas have never been cultured in vitro and identification is usually based on cytological examination of blood smears . However, this method has proven inefficient due to low sensitivity and specificity resulting in false negative results. Development of PCR based methods has increased the sensitivity of identification of the organisms in blood samples [9, 12, 16–18]. Feline infectious anemia was initially reported in Denmark in 1974, but the prevalence is unknown . In this study, we used PCR methods to determine the prevalence of feline haemoplasmas in the blood of convenience samples of cats from Denmark and evaluated possible associations to breed, age, gender and symptoms.