The Tgm9-insertion sites were mapped to single genes in each of the five mutants investigated (Tables 1 and 2; Fig 1). The five Tgm9-induced mutant genes identified in these five mutants are from previously mapped mutant loci or regions. For example, in three chlorophyll deficient mutants (y20 mdh1-n) a Tgm9 insertion was identified in the first intron of Glyma.12G159300 encoding lactate/malate dehydrogenase (Table 2). The y20 Mdh1-n locus was originally mapped to Chromosome 12 and shown to be flanked by microsatellite markers Satt253 and Satt302  (Table 1). Glyma.12G159300 is located in this y20 Mdh1-n genomic region. Similarly, the Tgm9-insertion site in the ms9 mutant was located in the first intron of Glyma.03G152300 that has no functional annotation (Table 2). The gene is in the ms9 region flanked by Satt521 and Satt237 (https://soybase.org) [30, 32] (Tables 1 and 2; Fig 1). The Tgm9-insertion sites in Fsp2 and Fsp3 were detected in the 7th intron and 2nd exon of Glyma.06G174200 and Glyma.08G359000, respectively (Table 2). Glyma.06G174200 encodes a haloacid dehalogenase-like hydrolase; Glyma.08G359000 encodes the embryo-specific protein 3, (ATS3) (Table 2) (https://soybase.org). The Fsp2 locus is flanked by Satt170 and Satt277 on Chromosome 6 . Glyma.06G174200 is located in this Fsp2 region (Tables 1 and 2; Fig 1). Likewise, the Tgm9 insertion sites in the fsp3 and fsp5 mutants were also localized to the genomic regions to which Fsp3 and Fsp5 were genetically mapped (Fig 1; Table 2).