Different tissue samples were collected and prepared as described by published report (Al Mardini et al., 1984). In brief, the whole blood was collected in heparin anticoagulated tubes, sealed in glass GC bottles, and then stored at −80 °C before use. The samples was placed on ice, weighed, and added to four parts Tris–HCl buffer (pH 7.0) before sonication. One milliliter of aliquots was subsequently added to septum bottles and treated in the same way. The DMS produced was stripped, cryo‐trapped, redissolved in water, and then analyzed by SPME‐GC‐MS. After removal of DMS by purge and cryo‐trapping, DMSO was reduced to DMS using NaBH4 and analyzed by SPME‐GC‐MS. Headspace sampling of DMS was carried out with a SPME holder equipped with a 75‐μm carboxen‐PDMS SPME fiber. Samples were incubated for 5 min at 35 °C and then extracted for 10 min at the same temperature. Then, the GC/MS analysis was performed with GC‐MS analysis using Agilent 6890N‐5975B GC‐MS system (Agilent Technologies, Santa Clara, CA, USA). The injector temperature was 200 °C, and injections/SPME desorptions were made in splitless mode. Helium was used as a carrier gas with linear velocity 40 cm s−1. The MS analyses were carried out in a SIM scan mode, with a scan of m/z 47 and 62. Electron ionization was used at energy of 70 eV. The temperature of the ion source and the transfer line was 190 and 150 °C, respectively. The acquisition of chromatographic data was performed by means of the Chemstation Software (Agilent).