Despite an increasing interest in Phasmatodea genetics, cytogenetic studies are not very frequent, compared to other model insects such as Drosphila. This may be due to problems in obtaining chromosomes, as laboratory cultures of stick insects often consist only of parthenogenetic females. Accordingly, karyotyping is not an easy task, as the most convenient and useful material for cytogenetic analysis in insects is male testes. Ovarioles can also successfully be used for chromosome preparation, but they contain only a few dividing cells—in our experience, only one of four laboratories used to prepare animal and/or specifically insect chromosomes, was able to obtain stick insect chromosomes for the present study.