In the present work, the case of a middle-aged woman with persistent infection caused by E. fecalis and the ultimate outcome motivated a development of a non-culture bacterial detection method in management of complicated patient cases. The objective was to further increase the knowledge regarding the detection of life-threatening silent infections. Anti-bacterial in vitro human antibodies were developed from patients at convalescence and immobilized in a SPR system and used for assessment of presence of bacteria in ulcer secretions. Compared to culture results, the produced antibodies could recognize the microorganism with quite high test performance.