Approximately 91% of total Treg had a demethylated TSDR before expansion (median, 91.1%). A significantly larger proportion of the CD25hi mTreg subpopulation showed a demethylated TSDR (median, 96.2%) compared with naive Treg (median, 83.5%) and CD25int mTreg (median, 84.6%) (Figure 1B).