Participants were instructed to fast for 8–12 hours prior to the blood collection. Blood specimens were analyzed for hemoglobin A1c (HbA1c), albumin:globulin ratio (A:G ratio), albumin, alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin (total), calcium, high sensitivity C-reactive protein (CRP), total cholesterol, creatinine, estimated glomerular filtration rate (eGFR), ferritin, gamma-glutamyl transferase (GGT), globulin, glucose, high density lipoprotein (HDL), cholesterol: high density lipoprotein ratio (CHOL: HDL ratio), total iron binding capacity (IBC), iron, low density lipoprotein (LDL), percent saturation (pctSat), protein (total), triglycerides, thyroid stimulating hormone (TSH) and uric acid. Hemoglobin A1c analysis was performed on the Cobas Integra 800. Both TSH and ferritin analysis were performed on the Siemens Centaur XP. C-reactive protein analysis was performed on the Dade Behring BNII. All other tests were performed on the Beckman Coulter Olympus 5800 platform. LDL analysis was performed as a direct LDL method. All reagents used were manufactured by the corresponding platform manufacturer with the exception of HDL which was performed using Roche reagents. Specimen analysis was performed by the Quest Diagnostics Lenexa Laboratory in Lenexa, Kansas. All allowable imprecision meets or does not exceed the College of American Pathologists (CAP) recommended allowable Total Error (TEa) for each assay.