Completed PARP1 cDNA would be acquired with primers (Table 1), and then we cloned completed PARP1 cDNA into pEGFP-N2vector to construct gene overexpression plasmid. As for the PARP1 gene silencing, shRNA and negative-control shRNA (shRNA-NC) of PRAP1 were designed according the sequence of PARP1 (NM_007415) from GenBank (Table 1). After annealing, shRNA of PRAP1 were ligated into pGPU6/GFP/Neo vector to build gene silencing recombinant plasmid. And then recombinant vectors were transfected into cell lines by liposome Lipofectamine 3000. Meanwhile, the blank vector transfection group was taken as the control group. After transfection for 2 days, there were part of green fluorescence cells which were converted to use 400 μg/ml G418 DMEM medium, screened for a month to attain gene silencing and overexpression stable transgenic lines, culture and cryopreserved in DMEM + 10% FBS + P.S. medium.