pLC447: This is a construct to tag proteins with RFP at the C terminus. CaCherry/RFP was PCR amplified from pLC43564 with oLC841 in combination with oLC842 and digested with BsrGI. This was cloned into pLC389 at BsrGI. Directionality of the insert was verified by PCR with pLC849 in combination with oLC842. The construct was sequence verified with oLC849 and oLC842.