HCWs are expected to mobilize clients for HCT but if they are unwilling to take the test themselves they may be ineffective in this role. The University College Hospital (UCH) in Ibadan is the foremost tertiary health facility and a major referral center for other tertiary facilities in Nigeria. It has various cadres of HCWs and trainees and a WHO reference laboratory for HIV. The proportion of HCWs who have been tested for HIV is unknown. There is little data on UCH HCWs’ willingness to participate in HCT. Other studies addressing attitudes of HCWs to HIV-positive patients in Nigeria have focused on nurses. Little information is available about the attitude of doctors, laboratory scientists and ancillary staff such as porters and hospital maids.