S. inexpectata had 44 chromosomes per metaphase plate including two pairs of large metacentric, and 20 pairs of acrocentric chromosomes of different sizes. Pericentric C-positive regions were present in all chromosomes. One arm of the largest metacentric chromosome pair was C-positive (Figure 3a). Two-color FISH showed that the C-positive region on this chromosome pair was enriched for 18S rDNA; telomeric repeats were also present in this region. The latter were dispersed along the heterochromatic region, but due to their small numbers they produced a FISH signal of lower intensity (Figure 3b–e). On other chromosomes, clusters of telomeric repeats were located at the chromosomal tips. Additionally, ITSs were observed in C-negative regions of some chromosomes (Figure 3c,e), and besides the classical clusters of telomeric repeats dispersed telomeric repeats were also observed in pericentric C-positive regions of some chromosomes (Figure 3c,e) of S. inexpectata. For the karyotype see Figure 2a.