Transfection of the novel LNA/DNA mixmers increases the production of SMN protein. (a) Western blotting of SMN and Cofilin of healthy and AON-treated SMA patient fibroblasts (GM03813). (b) Fold increase in SMN expression of AON-treated SMA patient fibroblasts. 5 nM transfection of AON 1–5 significantly increased the production of SMN proteins. Cofilin was used as a loading control. The data was normalized to the ratio of SMN/Cofilin in non-treated SMA fibroblasts. Bars represent mean ± S.D. of four independent experiments. One-way ANOVA with Dunnett’s multiple comparison test. M: mock control AON, NT: non-treated.