When adjusted for the number of input RNA copies based on the cycle-threshold (Ct) of the TaqMan matrix gene assay, H3N2 strains representative of those circulating in the 2012–2013 and 2014–2015 seasons had significantly lower average nAMPs than the H1N1 strain from 2013 to 2014 (see Table 2) and these average nAMPS were very close to those of the seasonal population averages. As was the case for the population averages, the average nAMPS of the TaqMan copy number adjusted H3N2 strains were statistically significantly lower than that of the H1N1 strain (p = 0.033 vs A/NY, p = 0.025 vs A/Texas and p = 0.017 vs A/Switzerland, t-test).