Mice IP immunized with the tag-less CFA/I/II/IV MEFA protein developed antigen-specific antibodies (Figure 7). Anti-CFA/I, -CS1, -CS2, -CS3, -CS4/CS6 and anti-CS5/CS6 IgG antibody titers in serum samples of the immunized mice were 3.5 ± 0.15, 3.4 ± 0.25, 3.4 ± 0.27, 3.5 ± 0.20, 3.3 ± 0.23 and 3.1 ± 0.20 (log10). No antibodies were detected to these adhesins from the control mouse serum samples.