Quantitative real-time PCR (qRT-PCR) was used to test the expression patterns of khdrbs in the different tissues of adult zebrafish. Total RNAs were extracted from the tissues gill, eye, brain, intestine, liver, heart, muscle, skin, spleen, testis, and ovary with TRIzolTM (Invitrogen) and purified using Total RNA Kit I (OMEGA Bio-Tek). For each tissue, 1 μg RNA was used for next reverse transcription. The cDNAs were reverse transcribed using M-MLV reverse transcriptase (TaKaRa) and Oligo (dT) primers as guided by the manufacturer’s instructions. β-actin and EF1-α were chosen as control to standardize the results by eliminating variations in mRNA and cDNA quantity and quality. qRT-PCR was conducted using the gene-specific primers (Table 2) on the ABI 7500 real-time PCR system (Applied Biosystem) with the 2× SYBR Premix Ex TaqTM Kit (TaKaRa). A total of 0.5 μl cDNA was used as template in each replicate. Reaction conditions were: 95°C for 15 s as stage 1, then 40 cycles of 95°C for 15 s, 60°C for 15 s, and 72°C for 35 s as stage 2. The expression level of khdrbs genes relative to that of the housekeeping genes β-actin and EF1-α was calculated by the comparative threshold cycle (CT) method (2-ΔΔCt). The experiments were performed in triplicate, and each replicate was from three experiments, i.e., the indicated replicates were both technical and biological replicates.