Effects of DNLA on the protein expressions of BDNF, CNTF and GDNF in brain tissues. (A) Protein contents of hippocampal tissues were plotted for sham, model and DNLA groups, respectively. The corresponding quantitation of BDNF, CNTF, GDNF protein were showed in (B–D) respectively. (E) Protein contents of cortex tissues were plotted for sham, model and DNLA groups, respectively. The corresponding quantitation of BDNF, CNTF, GDNF protein were showed in (F–H) respectively. The relative optical density was normalized to β-actin. Data were expressed as mean ± SEM (n = 3). Significance ∗P < 0.05, ∗∗P < 0.01 compared to the sham group, #P < 0.05, ##P < 0.01 compared to the model group.