Bovine enterovirus (BEV) is a single positive-stranded RNA virus belonging to the genus Enterovirus within family Picornaviridae. The viral particle is composed of a small, non-enveloped and icosahedral virion and 7.5 k-base genome containing a single open reading frame (ORF) flanked by untranslated regions (UTRs) at the 5′ and 3′ ends. The ORF encodes a single long polyprotein containing structural proteins (VP1, VP2, VP3 and VP4 encoded in P1) and non-structural proteins (2A, 2B and 2C encoded in P2 as well as 3A, 3B, 3C and 3D encoded in P3) [1, 2].