Adaptor has 5’ extended strand with no binding site for primers AP1 (Adaptor Primer 1) or AP2 (Adaptor Primer 2). Binding site for AP1 or AP2 can only be generated by transposon specific primers (TransR1 or TransR2). Exposed 3’ end of the adaptor is blocked by amino group to prevent extension. Unique bands from different lanes are excised and sequenced. The bands that are common to all lanes are most likely ancient transposition events.