10.7554/eLife.29118.013Figure 5—figure supplement 1.A leading strand block inhibits CMG unwinding even in the presence of Mcm10.Helicase reactions were performed as in Figure 5B except that the substrate contained a dual biotin-streptavidin block on the leading strand (50duplex LEAD2 dual biotin); the lagging strand (50duplex LAG2) was radiolabeled. Oligo sequences are in Table 1.