Of the 15 microsatellite primer pairs evaluated, 10 (SS20E, SS24F, Eari4-5, Eari4-6, CO189, Sg-2, Sg-8, ER-HAJZC, LHO35, and HSALZ-12) amplified fragments of expected sizes after optimizing the annealing temperatures. However, when these 10 loci were evaluated in the A. flaccida populations, over 80% of the individuals showed null alleles for all the loci in all the populations, indicating that these loci were not suitable for obtaining the genetic data of A. flaccida.