Toll-like receptors (TLRs) and sphingosine-1-phosphate-1 receptor (S1RP1) expression profiles in the target tissues of chickens infected with H5N6. At 3 days post-infection, the target tissues (i.e., brain, lung, spleen, and bursa of Fabricius) of H5N6-infected chickens were harvested for TLR and S1RP1 mRNA level detection via qRT-PCR method. (A) TLR3, (B) TLR7, (C) S1PR1. Data are expressed as M ± SD. Differences were analyzed with a paired Student’s t-test and were considered statistically significant at ∗p < 0.05, ∗∗p < 0.01 compared to control. B, Brain; L, Lung; S, Spleen; BF, Bursa of Fabricius.