Twenty scFv clones were randomly picked from the library to check the presence of scFv inserts and diversity of the scFv phage library. Colony PCR was done by using forward primer; PTfw 5′ CCT TTC TAT GCG GCC CAG CCG GCC ATG GCC 3′ and reverse primer; (pAK Sfi1) 5′ TCA GCA TGG CCC CCG AGG CCG CAC GTT TRAT 3′, PAK lambda Sfi 5′ TCA GCA TGG CCC CCG AGG CCG CAC CTA RRA C 3′ (R = G and A). Plasmid DNA of colony PCR positive scFv clones was isolated, sequenced commercially by Macrogen (South Korea), and the sequences were analyzed by online IMGT/V-Quest software provided by the international ImMunoGeneTics database (IMGT) (http://www.imgt.org/IMGT_vquest/share/textes/).