Although the mechanism of sex differentiation in crustaceans has yet to be defined, the androgenic gland (AG) is thought to be the exclusive organ that produces the androgenic hormone, which induces male sexual development. The AG system serves as a unique biological system for studying endocrine regulation of sex differentiation in decapods. GO and pathway analyses of DEGs will be helpful in the discovery of novel genes that play key roles in maintaining male characters and related behaviors during AG development.