Preparation of slides for 4,6-diamidino-2-phenylindole (DAPI) staining involved incubation of the slides at 60 °C to remove wax in which they were initially fixated within. This was for 30 minutes. They were then submersed three times with stock solution of xylene for 5 minutes each time. After this step, they were taken through absolute IMS for 5 minutes, followed by 90% IMS for 5 minutes and then 70% IMS. Finally, one drop of DAPI stain was applied. Three slides were prepared with DAPI for each time point from the different protocols and assessed under fluorescent microscopy (Invitrogen FL Cell Imaging System) (n = 3).