To further verify a role of Nrdp1 in OGD-induced apoptosis in neurons, we assessed the effect of Nrdp1 on several key apoptosis-associated signal proteins including cleaved-PARP and Bax/Bcl-2. As shown in Figures 3F,G, 6-h OGD induced a significant increase in cleaved PARP levels (PARP activation) and a greater ratio of Bax/Bcl-2 in cerebral cortical neurons, and transfection with Ad-si-Nrdp1 abolished these changes. Accordingly, overexpression of Nrdp1 augmented OGD-induced increases in cleaved PARP and Bax/Bcl-2 ratio. As expected, Ad-si-Nrdp1 or Ad-Nrdp1 alone did not affect these apoptosis-associated signal proteins (Figures 3F,G). Taken together, these results clearly indicate that Nrdp1 plays an important role in ischemia-induced apoptosis in cerebral cortical neurons.