To assign chromosomal locations to TaFlo2 genes, genome-specific primers and a set of Chinese Spring nulli-tetrasomic lines were used. The TaFlo2 genes TaFlo2-A1, TaFlo2-B1 and TaFlo2-D1 were found to be located on chromosomes 2A, 2B and 2D (Fig. 3). The cloned sequences of TaFlo2-A1, TaFlo2-B1 and TaFlo2-D1 showed >99% similarity with 2AL, 2BL and 2DL scaffolds (IWGSC_chr2AL_ab_k71_contigs_longerthan_200_6436403, IWGSC_chr2BL_ab_k71_contigs_longerthan_200_7959819, IWGSC_chr2DL_ab_k71_contigs_longerthan_200_9909583). Further analysis revealed that the TaFlo2-A1 gene was located on deletion bin ‘2AL1–0.85-1.00’.Fig. 3Assignment of TaFlo2-A1, TaFlo2-B1 and TaFlo2-D1 to wheat chromosomes 2A, 2B and 2D, respectively, by PCR mapping with the genomic DNA of Chinese Spring (CS) and derivative nulli-tetrasomic lines (N2AT2B, N2BT2A and N2DT2A). The size (kb) of DNA markers is shown to the left of the image