Behavioral changes after acute social aggressive stimulation. a Diagram of social aggression and social behavioral test. Social aggression is based on the resident (KM mouse)—intruder (C57B6/L mouse) paradigm. b Representative images of the track during social interaction. c Freezing time in the open field. On days 1 and 3 after stimulation, the freezing time of intruder mice was longer than that of control mice. On day 7 after stimulation, the freezing time of intruder mice was shorter than that at day 1 after stimulation but remained longer than that of control mice, one way ANOVA with Tukey’s test was used, F = 4.46, *p < 0.05, **p < 0.01 compared with control, n = 12. d Time spent in the social interaction zones. On days 1 and 3 after stimulation, intruder mice spent less time in the interaction zone than the control mice. On day 7 after stimulation, intruder mice spent more time in the interaction zone than at days 1 and 3 after stimulation but still spent less time in the interaction zone than control mice, one way ANOVA with Tukey’s test was used, F = 8.98, *p < 0.05, **p < 0.01 compared with control, n = 12