To list enriched-genes, we performed a hierarchic clustering analysis that also helped to see similarities between patients. The genome-scale H3K27me3 profile of each group was then compared. Figure 1 showed the enrichment of genes such as IFIH1, RCN1, XRN2, EIF2B, RP11-156P1.3 and AC079305.11. However, differences at the genetic and molecular level could explain by the interindividual difference in control group. Interestingly, all of these genes are shared with all patients in GS ≤ 7 group. Despite interindividual variability, we identified one gene that is specific to GS ≤ 7 group, TRA2A gene (Fig. 2).Fig. 1Hierarchical clustering on a set of 13 normal biopsies. The scaled enrichment score of individual patients is plotted in a red-yellow scale. Color intensity reflects magnitude of enrichment score, with red indicating high H3K27me3 enrichment and yellow indicating low H3K27me3 enrichment. Columns represent individual tissues. Rows represent the genes. The dendogram represents overall similarities in patient profiles Fig. 2Hierarchical clustering analysis of tumor tissues with Gleason score ≤ 7. The scaled enrichment score of individual patients is plotted in a red-yellow scale. Color intensity reflects magnitude of enrichment score, with red indicating high H3K27me3 enrichment and yellow indicating low H3K27me3 enrichment. Columns represent individual tissues and rows represent the genes. The dendogram represents overall similarities in patient profiles