The protein of gastric cancer tissues was extracted through RIPA Buffer (Aidlab) with protease and phosphatase inhibitor cocktails (Roche). BCA protein assay (KeyGen biotech) was used to quantify protein concentration. The protein (30 μg) was separated in 10% Tris Glycine SDS gels and transferred to polyvinylidene difluoride membranes (Millipore). The membranes were blocked with 5% milk in TBST for 60 minutes at room temperature, then incubated with primary antibodies overnight at 4°C. After incubating with secondary antibodies (ZhongShan Golden Bridge Biotechnology Co., Ltd), the membranes were tested with Super Signal West Femto Masimun sensitivity substrate (Thermo Scientific).