However, U87 loaded with miR-5096 mimic did not transfer calcein to HMEC monolayers (Figure 5B). Conversely, a large calcein transfer to HMEC, attesting the formation of heterocellular GJIC, was observed by inhibiting miR-5096 in U87. The GJIC capacity was increased by 4 fold with the miR-5096 inhibitor and was completely abolished with miR-5096 mimic (Figure 5B, right panel). We performed the same experiment by transfecting HMEC with miR-5096 mimic and inhibitor. Two days after transfection, loaded HMEC were plated onto HMEC monolayers and the calcein transfer was observed (see Supplementary Figure 2). Neither miR-5096 mimic nor inhibitor affected the GJIC established between transfected and non-transfected HMEC.