Two different strains named CNPV-PD1 and CNPV-PD2 (Table 2) were identified in house sparrows from southern Spain (proportions 8:1 and 7:7 in 2013 and 2014). Only the strain CNPV-PD2 was found in house sparrows captured in central Spain. The phylogenetic analysis, which included 66 sequences of 448 nucleotides in length (some data bank sequences were shorter than the amplicon from this study), shows CNPV-PD1 and CNPV-PD2 placed in two subclades B2 and B1, respectively; both Canarypox-like viruses (Fig 2). CNPV-PD1 was the predominant sequence in southern Spain in 2013 (100% in natural and 83% in agricultural areas), but it was less common in 2014 (50% in natural and urban areas), though this variation was not statistically significant. This strain shows 100% identity to an APV from an american flamingo (Phoenicopterus ruber ruber) at Lisbon Zoo, Portugal (HQ875129) [a houbara bustard (Chlamydotis undulata) in Morocco (LK021660), a great bustard (Otis tarda) in Hungary (KC018066)  and shares 70% similarity to FWPV and CNPV. This sequence shows two amino acid insertions and one deletion at the N-terminal sequenced region compared to all other APV strains. CNPV-PD2 clusters with CNPV and has been found in Passeriformes and other bird orders in Europe and USA (Fig 3).