In this study, we demonstrated that PA-MSHA inhibited the proliferation of two pancreatic cancer cell lines (PANC-1and SW1990) in a time- and dose-dependent manner. Treatment of pancreatic cancer cells with PA-MSHA resulted in cytoplasmic condensation, cell body shrinkage, nuclear fragmentation, disorganized cellular structures, and the detachment and fusion of the endoplasmic reticulum with the plasma membrane to form multiple vacuoles. These morphological changes indicated PA-MSHA induced apoptosis in pancreatic cancer cells.