The area of the vessel adventitia was manually removed externally bypassing the muscle layer, which is easily visible. The automatic deletion was cutting structures of the muscle layer when some areas of adventitia had the same or similar color intensity that the middle layer (muscle);The remaining image was transformed into 8-bit;The rest of the image formed by the lumen of the vessel and myointimal layer was transformed into a mask, and the area in pixels was measured; this area was called the total area;Then, only the area of the vessel lumen was measured;The area of myointimal layer was calculated in pixels by subtracting the light area from the total area (area of the mean-intima layer with light);The percentage was then calculated by dividing the area of the myointimal layer by the total area.