H3K27me3 sites were defined as differentially enriched if the Enrichment Score was >1.5, and for each H3K27me3 site the mean Enrichment Score level was compared in tumor tissue group versus normal tissue. Factorial discriminant analysis (FDA) and ANOVA were performed to discriminate the three groups. Data was analyzed using R statistics. Thresholds set for statistical significance were *p < 0.05 and **p < 0.01.