Assessing the neuroinflammatory response and brain parasite burden. A. The neuroinflammatory response scores were analysed in uninfected animals (n = 4) and animals killed at 7, 14, 21 and 28 days post-infection (n = 5 per group). An increase in the severity of the reaction can be seen as the infection progresses. B. The number of copies of the parasite specific PFR-2 gene in a 100 ng sample of DNA extracted from the brains animals killed at 7, 14, 21 and 28 days post-infection (n = 5 per group) was measured by Taqman PCR. The trypanosome burden within the CNS increases as the disease progresses with a significant increase in parasite load detected at 14 days post-infection compared to the earlier time-point. A and B. Dots represent the group mean, bars represent one standard error of the mean. The means of groups that do not share a letter are significantly different (p < 0.05) from each other.