Autophagy-specific assays show IVY1 and ATG5 suppress the temperature-sensitive autophagy defect of the ypt6Δ strain. (A) GFP-Atg8 degradation was increased at 37° in ypt6Δ when transformed with plasmids containing IVY1, ATG5, and WT YPT6. (B) IVY1 and ATG5 increased the Pho8Δ60 activities in ypt6Δ (YSA004) at 37°. Error bars represent SD from three biological replicates. Methods are the same as in Figure 1. GFP, green fluorescent protein; WT, wild-type.