Often, vines with symptoms on the canes also developed fruit symptoms. On immature clusters, black spots appeared on the berry surface and lesions on the rachis were also observed (Fig. 1c, d). After veraison, rachis lesions became dry and blackened, and berries became shriveled or split (Fig. 1c). Cluster symptoms were absent from female parents and were not possible to observe with the dioecious male parents V. cinerea B9 and Illinois 547-1. Among progeny, cane symptom scores correlated with the presence of cluster symptoms, with Pearson’s r of 0.92 and 0.76 in 2012–2013 and 2013–2014, respectively (Fig. 1d). The typical leaf spot symptom was rare among all families. Samples from symptomatic dormant canes incubated in humid conditions developed pycnidia that exude conidia (cirrhi), typical of D. ampelina. Conidia from these samples were successfully cultured on PDA plates, producing colonies with typical growth rings and cream colored cirrhi of pycnidia (Fig. 1b). Isolation of fungi was not successful from symptomatic berries.