Sediment samples were collected using a van Veen grab from varying depths (50–250 m) in the Arctic Fjord ‘Kongsfjorden’ located in Ny-Ålesund, Svalbard in, July 2012. The samples were serially diluted in ice-cold sterile seawater and plated on Actinomycetes Isolation Agar (AIA) (Himedia, India) supplemented with 0.2 mg/L gentamycin, 0.25 mg/L cycloheximide, and 0.1 mg/L amphotericin B. These were incubated at 20 °C for 4 weeks at which time characteristic actinobacterial colonies were isolated and their extracts screened for anticancer activity as described below.