We diluted all of the cDNA eluent to 200 ng/μl, checking (using a NanoDrop 1000 Spectrophotometer) that the 260:280nm absorbance ratio were all between 1.5 and 1.8 before sending 10 μl samples to the Ramaciotti Centre for Genomics, University of New South Wales, for Fluidigm quantitative RT-PCR gene expression analyses . Fluidigm RT-PCR gene expression analysis utilizes dual-labelled probes designed to hybridize a complementary region of the cDNA for real-time amplification. These probes contain a fluorescent reporter dye on the 5' base, and a quencher on the 3' base, whose intensity increases proportional to the number of probe molecules cleaved .