Validation of EGFR and HER3 expression in AsPC-1, BxPC-3 and Mia PACA2 pancreas cancer cells was conducted via standard flow cytometry and immunoblot analysis. Cells were labeled with anti-EGFR-AF488 (AY13, Biolegend) and anti-HER3-APC (1B4C3, Biolegend) and analyzed for receptor expression using BD LSR II FLOW cytometer (BD Biosciences).