To identify microsatellite primer pairs to be evaluated for transferability to A. flaccida, a literature review was first carried out to identify primer transferability studies among Asteraceae species. Of the primer pairs identified, 15 were selected based on the phylogenetic proximity of A. flaccida to the species for which the primers were initially developed (Table 2). Preference was given to primers pairs that amplified a higher number of alleles and showed transferability to other Asteraceae species as reported in previous studies (Table 2). All of these primers pairs were designed to amplify regions of genomic DNA.