Experimental Phase plot size ranged from 12 to 384 m2, and Pilot Phase plot size ranged from 398.7 to 4128 m2 (Supplementary Table 2). The main soil textures varied across locations and included clay, silty clay, clay loam, sandy loam, sandy clay loam, and sandy silt. Row spacing varied from 0.65 to 0.92 m, with a seeding rate of 5 to 10 seeds per meter and seed planting depth of 2 to 9 cm. Plot management was according to the recommendations by INIFAP-CIRNO for maize (Mendoza et al. 2003). Crop management practices included seedbed soil preparation, fertilization, irrigation, and insect and weed control according to regional best practices. Agronomic practices were conducted uniformly across all entries within a study in the Experimental Phase trials in order to eliminate an additional source of variation on the agronomic and phenotypic characteristics. However, in the Pilot Phase trials, insect and weed control practices were conducted according to each material’s phenotype, i.e., the IR/HT hybrids MON-89Ø34-3 × MON-88Ø17-3 and MON-89Ø34-3 × MON-ØØ6Ø3-6 GM did not require conventional insecticide applications for target lepidopteran insect pests, but MON-ØØ6Ø3-6 (HT only) and the conventional hybrid required two to four application of conventional insecticides to control lepidopteran pests across most sites. Weed control was also different between the GM hybrids (all HT) and the conventional control hybrid. Across all sites, one or two over-the-top applications of Faena Fuerte® with Transorb® 1 (540 g a.i. L−1), a glyphosate-containing herbicide, were made on the three GM hybrids at rates of 2 to 4 L ha−1. Weed control for the conventional control was mechanical (cultivator or manual) and/or by applications of selective herbicides. Subsequently, weed control was evaluated at approximately 11, 20, and 30 days after herbicide treatment in all of the studies.