Whole-genome shotgun sequencing was performed using a combination of the PacBio RSII (Pacific Biosciences, Menlo Park, CA), Illumina HiSeq/MiSeq (Illumina, San Diego, CA), and Argus (OpGen, Gaithersburg, MD) platforms, as described previously (10). Briefly, genomic DNA libraries were prepared for PacBio sequencing using the SMRTbell template prep kit 1.0 and polymerase binding kit P4, while Illumina libraries were prepared using the NEBNext Ultra library prep kit (New England BioLabs, Ipswich, MA). De novo genome assembly of filtered reads was performed using the Hierarchical Genome Assembly Process (HGAP version 3; Pacific Biosciences) at 130× and 144× coverage for B202 and B203, respectively. The resulting consensus sequences were determined with Quiver (version 1), manually checked for circularity, and then reordered to match the start of reference strain Tohama I (accession no. CP010964) (10). To ensure accuracy, assemblies were confirmed by comparison to BamHI and KpnI restriction digestion optical maps using the Argus system (OpGen) with MapSolver (version 2.1.1; OpGen) and further polished by mapping either Illumina HiSeq PE-100 or MiSeq PE-300 reads using CLC Genomics Workbench (version 8.5; CLC bio, Boston, MA). Final assemblies were annotated using the NCBI automated Prokaryotic Genome Annotation Pipeline (PGAP).