SuperScript III First-Strand Synthesis System kit (Invitrogen) was used to synthesize cDNA, with the detailed processes shown in brochure. The fluorescent primers were designed using primer premier 5.0 according to the sequence of genes. Real-time PCR measurements were performed on CFX96 (Bio-Rad). Each PCR reaction contained SYBR® Premix Ex Taq (2x) 12.5 μl, 10 μmol/L upstream and downstream primers each 1 ul, cDNA template 2 μl, dH2O 10μl. The temperature process was 95°C for 30 sec followed by 40 cycles of amplification (95°C for 5 sec, 60°C for 30 sec, and 72°C for 30 sec). 2−ΔΔt was adopted for date processing.