Images of five non-overlapping areas from each section stained for F4/80 or CD3 were obtained with a digital camera at a magnification of 200×, and the percentages of the areas positive for each staining were measured using image analysis software (LuminaVision ver. 2.04, Mitani Corporation, Tokyo, Japan) and averaged. For WT-1 staining, 20 glomeruli were assessed, and the numbers of WT-1-positive cells per glomerulus were counted and averaged.