The presence of the 2f1-f2 component after pure tone stimulation was evident in both groups in DPOAE studies. However, FD-10M mice presented increased DP thresholds at all the frequencies studied as compared to NF-10M mice, the differences being significant at 8–14 kHz (Figure 2B). Concomitantly, decreased 2f1-f2 amplitude was detected in the FD-10M group, which became significant at 10 kHz (Figure 2Bb). These results suggested that the initial signs of hearing loss and the outer hair cell damage were more pronounced in the FD than in the NF group after 8 months of nutritional intervention.