Persistent infection in biofilms has been the subject of clinical studies since 1981. Biofilms are a collection of microbes that adhere to surfaces by producing a matrix that shields them from environmental elements. It has been speculated that bacteria colonizing chronic wounds are part of the highly persistent biofilms . Molecular analyses of chronic wound specimens revealed diverse polymicrobial communities but it has been very difficult to identify specific bacteria of the entire individual, especially strictly anaerobic bacteria, by culture methods . Traditional culturing methods may be extremely biased as a diagnostic tool as they select for easily cultured organisms e.g. S. aureus, but not bacteria difficult to culture such as anaerobes . The formation of bacterial biofilms could lead to chronic inflammation. Detachment of the biofilm enables bacteria to enter into the blood stream, causing bacteremia and vascular embolism . The establishment of a non-cultural method for analysis of infections may help to identify the key bacteria that cause pathogenic biofilms [5–7]. Thus, it is most important to identify the underlying causative infectious agent in the symbiotic community of biofilms in order to antagonize the development of a suitable environment for opportunistic pathogens and thereby improve the wound healing process.