We analyzed the effect of miR-5096 on Cx43 expression in the two cell types (Figure 3C). Transfection of U87 with a miR-5096 mimic induced a 2-fold increase in Cx43 expression in U87, without modifying Cx43 expression in co-cultured HMEC for 12 h (Figure 3D). This is in agreement with the large transfer of miR-5096 from U87 to HMEC within 12 hours of co-culture (Figure 2C, 2D) and its inhibition by decreasing Cx43 expression using siRNA in U87 (see supplementary Figure S3C).