The germplasm collection of CREA-UTV in Turi (Bari, Italy) (Lat. 40°92 57′24.54″N, Lon.17° 00′28.94″E) includes internationally spread varieties of both table and wine grapes and consist of about a thousand of unique genotypes. The collection includes autochthonous varieties of the Southern Italian regions (Basilicata, Calabria, Campania, Apulia and Sicily). A sup-population, consisting of 107 most diffused and cultivated varieties, have been selected as best representing South-Italian grapevine biodiversity and further characterized in this study.