FPKM (fragments per kilobase per transcript per million mapped reads) method was performed to quantify the expression of two expression profiles . The False Discovery Rate (FDR) method was applied in hypothesis testing and multiple hypothesis testing to calibrate significant levels and eliminate influence of random fluctuations and errors . At the same time, according to the gene expression level (FPKM value), differentially expressed multiples in different samples were calculated. The differentially expressed genes (DEGs) were defined as FDR ≤ 0.001 and no less than 2-fold.