Sacred lotus (Nelumbo nucifera, Gaertn.) is a basal eudicot which is of significant cultural, agricultural, medicinal, as well as religious importance. TPS plays important role in plant response to environmental stimuli, and thus the study of TPS would be very important for lotus breeding and the research of stress-resistance mechanism in lotus. In this study, we investigated the lotus TPS genes from whole genome-wide studies and their evolutionary relationship with other TPS genes. We explored TPS gene family members' expression patterns in various tissues and in response to various stresses. Having conducted a thorough analysis of gene sequences, molecular evolution, gene structures, and gene expression patterns, we offer an effective framework for further functional characterization of lotus TPS gene families.