The nuclear magnetic resonance (NMR) spectrum–based cellular metabolome analysis is a novel method to assist radiosensitization study in tumor radiation therapy. 1H NMR analysis is an ideal high throughput method to detect small-metabolites in biological samples [10, 11]. With pattern recognition, 1H-NMR-based metabolome analysis screens differentially expressed metabolites between different samples . Followed by bioinformatics analysis on the basis of a public database, the changed metabolic pathways can be further identified.