In order to identify the main pathways involved in aging-related lesions, high throughput RNA analyses (RNA-Seq) have been in performed in WT and CalpTG mice renal cortex. Differential expression analysis and pathways analysis revealed that most of differentially expressed pathways were related to immune cell response and that many cytokines involved in innate immunity and particularly in inflammasome activation were differentially regulated between the 2 groups (Fig. 9A,B).Figure 9Genes differentially expressed in aged WT and CalpTG mice are related to inflammatory pathways. Heatmap representation of genes differentially expressed between old WT and CalpTG (TG) mice. Eighty-two renal-expressed transcripts have been found significantly differentially expressed between groups. Corresponding genes were represented using the Pheatmap R package, unsupervised clustering, Manhattan method (A, n = 6/group). Kegg and biocarta pathways found significantly enriched when comparing old WT and CalpTG mice (B). Pathways are immune related suggesting that large parts of difference observed are associated with inflammaging-related genes.