The ANOVA of alpha-2 responses revealed significant differences for training, F(3,72) = 3.30, p = 0.025, ηP2 = 0.12. Post hoc comparisons showed that the spontaneous EEG alpha-2 power was significantly higher after physical Qigong training compared to mental practice, p = 0.03, video control, p = 0.02, and baseline rest, p = 0.01. No difference was obtained between mental practice, video control, and baseline rest. The ANOVA of alpha-2 responses revealed highly significant differences between experimental conditions (eyes-open, eyes-closed), F(1,24) = 13.38, p = 0.012, ηP2 = 0.36. The ANOVA of alpha-2 responses revealed no significant results for training × condition. The ANOVA of alpha-2 responses revealed significant differences between locations, F(4,96) = 3.93, p = 0.005, ηP2 = 0.14. Post hoc comparisons showed that the spontaneous EEG alpha-2 power at central, and parietal electrodes was higher than that of frontal, p < 0.05 each, temporal, p < 0.05 each, and occipital electrodes, p < 0.05 each.