The heat treatment temperatures (50, 60 °C) and heating time (5, 10 min) with a PVCT ratio of 2.4 were applied during the forming process of a negative Poisson’s ratio. Poisson’s ratios of experimental group were 0.05 at 50 °C/5 min, −0.03 at 50 °C/10 min, −0.04 at 60 °C/5 min and −0.07 at 60 °C/10 min as shown in Fig. 6, respectively. The result showed that PLGA50:50 scaffolds specimen depended on heating temperature and heating time to reveal negative Poisson’s ratio. The lowest negative Poisson’s ratio was −0.07 with 60 °C/10 min at 10 % strain (see Fig. 6). These results are important because the different heating temperature and heating time for the PVCT ratio would affect the negative Poisson’s ratio.Fig. 6Poisson’s ratio variations for control and experimental specimens. Processing was done at 50, 60 °C and for 5, 10 min with a volumetric compression ratio of 2.4 (n = 4, *p < 0.05)