A segment of the mouse Foxo1 gene promoter spanning nucleotides −880 to −751 relative to the transcription initiation site was amplified by PCR using the following primers: Forward, 5′-CAGGATCCTGCGACTTCAACACCTCATC-3′; and reverse, 5′-CAAAGCTTCGGTGTGGTGGCTAAAGAGT-3′. The resulting fragment was cloned into the luciferase reporter plasmid pT81Luc70 to yield Foxo1T81Luc. The expression vector pcDNA3-Alx3 has been described elsewhere45.