BER is necessary for induction of AREG and EREG expression downstream of integrin α6β4 signaling. (A,B) RNA was isolated from Panc1-3D7 cells stably expressing non-targeting or shRNA specific for TET1. QPCR analysis was used to confirm TET1 knockdown (A) and expression of AREG and EREG (B). (C) Nuclei were isolated from Panc1-2G6, Panc1-3D7, and Panc1-3D7 cells expressing specific lentiviral shRNA for TDG. Western blot analysis was performed on nuclear fractions for TDG and Lamin A/C used as a loading control. (D) Cells were collected and AREG and EREG expression measured by QPCR. (E,F) Cells were treated with either 1 μM or 10 μM 3,4-Dihydro-5-[4-(1-piperidinyl)butoxyl]-1(2 H)-isoquinolinone (DPQ) for 72 hours. Expression of AREG and EREG was measured by QPCR in Panc-2G6 (low α6β4; E) and Panc-3D7 (high α6β4; F) cell lines. Data depicted are representative of at least three different experiments and represent the mean +/− standard deviation. Statistical significance was calculated using a one-tailed t-test in which *denotes P < 0.05 as compared to controls.