Each region of the GI tract was analysed for differences in community composition. Analysis did not reveal any tissue specific fungal communities, but sequences similar to the Saccharomycete genus Metschnikowia were found exclusively in the foregut of both fish while OTUs with sequences similar to Tremellomycetes and the Agaricomycete genus Stereaceae were found solely in the hindgut. These OTUs were not found in high abundance in either feeding regimen suggesting limited biological significance. However, analyses were based on the sequences of one foregut, midgut and hindgut from each diet and may not reflect actual variability amongst individuals .