Further, we evaluated the levels of cleaved caspase‐3, a marker of apoptosis, in normoxic and H/R cultures with and without OEC‐CM. We found that cleaved caspase‐3 levels were significantly higher in hypoxic cultures, at all reoxygenation times, compared to normoxic cultures (Fig. 2B). Cells grown with OEC‐CM, in normoxia and exposed to H/R, did not show significant differences of cleaved caspase‐3 levels compared to unconditioned cultures (Fig. 2C).