5) The electron cryo-tomography of the prepore reveals a structure of 34 protomers in the prepore, a number expected based on numerous other reports. However, the CryoEM structure of the pore reveals a larger 42 protomer pore. This is unexpected but interestingly the authors note a range of ring structures were present with this being the most stable. So the question arises whether the pore structure is an artifact. Can the authors model a 34 protomer pore based on their 42 protomer structure and convince that the details they discuss in the 42 protomer pore are likely relevant to the smaller pore? Is the 20º incline in the β-hairpin maintained in modeling the smaller pore? Like the authors, other workers have found that PLY form linear oligomers in their crystals. The authors should discuss how linear oligomers fit into their detailed mechanism of pore formation.