Fourier transform-infrared spectroscopy (FTIR) of native cellulose and yielded nanocellulose were analyzed using a FTIR spectrometer (Bruker IFX 66/S, (PerkinElmer, Germany)) in order to determine the functional groups of the cellulosic product. In fact, the effects of the hydrolysis treatment on the chemical composition changes can be tracked from the FTIR spectra. The FTIR analysis was carried out in the transmittance mode with a wavelength range of 4000–400 cm−1 with a resolution of 4 cm∓1 at 32 scans. The samples were ground and mixed with KBr powder with a ratio of 1:100 (w/w), then pressed into an ultra-thin pellet before the analysis.