We also observed whether re-introduction of exogenous BECN1, SQSTM1 or ATG16L1 could provide resistance to miR-20a-mediated autophagy inhibition and prevents DNA damage. Both immunoblotting and DQ Red BSA fluorescence assay showed that overexpression of BECN1, SQSTM1 or ATG16L1 construct was sufficient to rescue miR-20a-induced inhibition of autophagy and lysosomal proteolytic pathway (Figures 5h and j). Additionally, miR-20a-induced γH2AX accumulation was markedly reduced by exogenous BECN1, SQSTM1 or ATG16L1 in nutrient-starved cells (Figures 5h and i).