Amplification of CsMRP4-NBD1 (a) and purification of the recombinant protein (b). Abbreviations: M, molecular marker (kDa); U, uninduced total lysate; I, induced total lysate; S, urea-treated clear supernatant; PT, Ni-NTA pass-through fraction; W, last washing; Elute 1–6, 1st to 6th fraction eluted from an Ni-NTA column. (TIFF 2938 kb)