ITC analysis of the LPS–fibrinogen interaction. (a) Titration of 8.8 μM human plasma fibrinogen (black circles) or buffer (green open circles) into 100 µM of E. coli O111:B4 LPS. (b) Titration of 50 ng µl−1 LPS (2.5 µM) or buffer into 3 µg µl−1 fibrinogen (8.8 µM) or buffer as indicated. Experiments were conducted at 37°C in phosphate buffered saline. (Online version in colour.)