Phylogenetic trees based on the nt sequences of 5′UTR and 3′UTR and aa sequences of P1, P2, P3 and VP1 are shown in Fig. 2 and Fig. 3, respectively. Phylogenetic analysis showed that all Japanese strains of BEV including BEV-AN12 formed a cluster with other BEVs in all six trees. However, BEV-AN12 was completely separated from other BEVs in the P1 and VP1 trees.Fig. 2Phylogenetic trees using nucleic acid sequences of 5′UTR and 3′UTR. Phylogenetic trees were constructed using nucleic acid sequences of EV-A to EV-J and Japanese BEVs based on the maximum likelihood method in MEGA5.22 with bootstrap values calculated for 1000 replicates. Scale bar indicates nucleotide substitutions per site. BEVs in Japanese were indicated as ■. Simian sapelovirus (AY064708) was used as outgroup Fig. 3Phylogenetic trees using amino acid sequences of P1, P2 and P3 and VP1 proteins. Phylogenetic trees were constructed using amino acid sequences of EV-A to EV-J and Japanese BEVs based on the maximum likelihood method in MEGA5.22 with bootstrap values calculated for 1000 replicates. Scale bar indicates amino acid substitution per site. BEVs in Japanese were indicated as ■. Simian sapelovirus (AY064708) was used as outgroup