Preparation and labelling of retina cryostat sections was performed as described previously9798. Briefly, 18 μm sections were permeabilised with 0.2% Triton-X100 (Sigma) for 20 minutes, blocked with 10% normal donkey serum (Sigma) before incubation with primary antibodies for 24 h at 4 °C. Details of all antibodies used are shown in Table 3. Secondary antibodies were donkey anti-rabbit, donkey anti-goat and donkey anti-mouse labelled with Alexa488 and Alexa568 fluorophores (Life Technologies) incubated at 1:200 for 2 h at RT. All antibodies were diluted in PBS containing 2% normal donkey serum and 0.2% Triton-X100. Sections were mounted with Prolong Gold anti-fade media containing DAPI (Life Technologies).