Electroporation with shRNA-IGF-1R (shIGF-1R) significantly reduces expression of IGF-1R. (a) Representative images of brains electroporated at E15 with control shRNA (top), shRNA-IGF-1R (middle) or co-electroporated with IGF-1R OPT and shRNA-IGF-1R (bottom) showing the expression of IGF-1 R (at E17). The ventricular zone (VZ) is labeled for orientation. All brains were co-electroporated with CAG-GFP. (b) Quantification of the number of electroporated cells positive forIGF-1R as shown in A. Note the significant decrease of IGF-1R expression in the cells electroporated with shRNA-IGF-1R. Student’s t test. ***p ≤ 0.0001, ns = not significant. n = 3 independent experiments. At least 100 cells were scored for each condition.