Immunofluorescence staining for the invadopodia constituent proteins cortactin (A, E), actin (B, F) and paxillin (C, G) in representative KPC and 17KPC cell lines. Merged images are shown in (D) and (H). D′ and H′ are higher magnification views of panels D and H. Scale bar = 10 um. Quantification of invadopodia rosettes is shown in (I). n = 3 for each cell line. Areas of FITC-gelatin degradation, identified as dark regions, are shown for representative KPC and 17KPC cell lines (J, L). (K, M) Co-staining for actin and DNA (DAPI). (N) Quantification of gelatin degradation. n = 3 for each cell line. Error bars represent standard error of the mean. p values: * < 0.05, ** < 0.01, **** < 0.0001.