While illuminating, current imaging-based structural analyses of AHO pathways were limited by understanding the relevance of the anatomy observed. It was unclear if large lumens necessarily represent increased or decreased AHO. The trabecular meshwork itself, oft described as a hypo-reflective interface shadow could connect to SC and be confusingly included as a portion of the AHO pathways . Structure-to-structure correlation of OCT to histology in the anterior segment has not yet reached the stage of what has been done in the posterior segment and retina [41–43]. Therefore, the biological relevance of the AHO pathway motion is unclear.