Representative Western blots showing levels of cytochrome c, BAX, and Bcl‐2 were examined in mitochondrial fractions by immunoblotting in hSOD1‐WT‐ and hSOD1‐G93A‐expressing NSC‐34 differentiated cells under serum starvation in the presence or absence of P110 (1 μM/24 h); VDAC. Protein levels were quantified and presented as fold change of hSOD‐1 WT.Representative Western blots showing levels of autophagy‐associated proteins; Parkin and LC3BII examined in mitochondrial fractions. Representative Western blots showing phosphorylated‐JNK, total JNK, p62, and LC3BII total levels in hSOD1‐WT‐ and hSOD1‐G93A‐expressing NSC‐34 differentiated cells under serum starvation in the presence or absence of P110 (1 μM/24 h); VDAC and β‐actin were used as a loading control for mitochondrial fraction (top panels) and total fraction (lower panels), respectively. Protein levels were quantified and presented as fold change of hSOD‐1 WT.Representative Western blots showing levels of ER stress‐associated protein, GRP78, CHOP, in hSOD1‐WT‐ and hSOD1‐G93A‐expressing NSC‐34 differentiated cells under serum starvation in the presence or absence of P110 (1 μM/24 h); β‐actin was used as a loading control. Protein levels were quantified and presented as fold change of hSOD‐1 WT.Data information: Mean, standard deviation, and P‐values are shown. n = 3 performed in (A) duplicates or (B, C) quintuplet; probability by one‐way ANOVA (with Tukey's post hoc test).Source data are available online for this figure.