DENV-1 to -4 infection downregulates the expression of endogenous miR-484 and miR-744 at the early stages of infection - To better evaluate changes in miR-484 and miR-744 expression, Vero cells were challenged with DENV-1 to -4 at an MOI of 3 or were mock-infected and the expression level of miR-484 and miR-744 was then evaluated at 8, 16, 24, 32, 48, and 72 hpi by RT-qPCR. These stages were considered as early (8-16 h), medium (16-48 h), and late (48-72 h) time-points post-infection. The Ct values were normalised to an uninfected control and to the 18S rRNA (∆∆Ct) to obtain the fold change in expression, according to the manufacturer’s instructions; as previously reported an RQ (relative quantification = 2-∆∆Ct) was considered significant at a minimum of two-fold change. The RT-qPCR results show that miR-484 or miR-744 expression was modulated after DENV infection; indeed, as shown in Fig. 4A-D, miR-484 and miR-744 were downregulated at early time points with all DENV serotypes, and depending on the DENV serotype this level was either maintained or increased progressively at the medium time-points, and finally increased progressively at the late time-points post-infection, except for the level of miR-744 in response to DENV-2 and DENV-4 infection. Taken together, these results suggest that all four DENV serotypes can modulate the endogenous expression of miR-484 and miR-744 at different time-points of infection.