RNA was extracted with Trizol (15596018, ThermoFisher Scientific) according to manufacturer’s instructions and cDNA synthesis was performed with the High Capacity Reverse Transcriptase Kit (4368813, Life Technologies). cDNA was amplified relative to GAPDH using the Forget-Me-Not qPCR Master Mix with Rox (31042–1, Biotium) according to the manufacturer’s protocol.