In addition, we detected the dimeric form by other approaches as well such as sandwich enzyme-linked immunosorbent assay (ELISA)8, size exclusion gel chromatography4 and isothermal titration calorimetry4. Now we have performed sandwich ELISA experiment with the N- and/or C-terminal free forms as earlier with the full length protein to establish the role of the unstructured terminal segments in the dimerization of the TPPP/p25. To validate the in vitro data obtained with isolated protein variants, we have performed in vivo experiments as well using bimolecular fluorescence complementation assay (mVenus BiFC), similarly as described in the commented paper1.