To examine the changes in melanocytes caused by fibroblasts with SCF gene alteration, the genes related to melanogenesis were analyzed. After the fibroblast cells with or without SCF gene were treated with or without UVB, the conditioned medium was collected after 24 h culture and then used to treat melanocytes for 24 h. The experimental conditioned medium obtained from fibroblasts was divided into four experimental groups: fibroblasts without both UVB exposure and SCF inhibition, with only UVB exposure, with only SCF inhibition, and with both UVB exposure and SCF inhibition. These secretions were used as culturing mediums for melanocytes to analyze their different expression levels when cultured in mediums with SCF or without SCF. In Figure 3, SCF gene knockdown, the two conditioned medium groups including the one from regular fibroblasts and SCF-silenced fibroblasts, were used to treat melanocytes separately. The gene expression level of tyrosinase and Pmel17 increased, while MITF, ERK-1, ERK-2, myosin Va (Myo5a), and c-kit decreased. The genes of tyrosinase, ERK-1, ERK-2, Myo5a, and c-kit were upregulated when treated in the conditioned medium with UVB compared with the homeostatic condition. In both treatments of UVB exposure with SCF inhibition group, the data showed the alterations in gene expressions in melanocytes when the conditioned medium was used for treatment. Among them, tyrosinase, MITF, ERK-1, ERK-2, Myo5a, and c-kit were upregulated, and Pmel17 was downregulated when the conditioned medium from fibroblasts undergoing UVB exposure and SCF silencing was applied to treat melanocytes, compared with the same medium with only UVB stimulation. These findings indicated SCF silencing caused the variations in melanocytes and regulated the melanogenesis-related gene expression using the conditioned medium treatment method.