Supaglutide attenuates body weight gain in DIO mice. (A) Body weight gain of the PBS- and supaglutide- treated mice was recorded and expressed as the percentage of weight at the day before first injection. Significant difference between control and SPG is determined using two-way RM ANOVA method. (B) Weight of tissues. Significant difference between control and SPG is determined using unpaired Student's t-test. All values are means ± SE (n = 5 for each group). *p < 0.05; **p < 0.01; ***p < 0.001.