Successful construction of the eukaryotic expression plasmid pEGFP-C1-CsGRN. a Restriction enzyme identification of the recombinant plasmid pEGFP-C1-CsGRN. DNA ladder 5000 (Lane M), double enzyme digestion of pEGFP-C1-CsGRN (Lane 1), recombinant plasmid pEGFP-C1-CsGRN (Lane 2), empty vector pEGFP-C1 (Lane 3). b Sequencing data from recombinant plasmid pEGFP-C1-CsGRN and CsGRN gene were completely matched. (TIF 206 kb)