Interestingly, effect of liraglutide on food intake in mice is only eliciting an anorexic effect on the first day of treatment. This is not the case for rats, which show a multiday anorexic response that is the primary driver of the weight loss . Despite the anorexic effect of liraglutide disappearing after the first day of treatment in mice, the body weight loss induced by liraglutide persisted for 4 days. These findings suggest that increased energy expenditure is involved in maintaining weight loss induced by liraglutide in mice. Liraglutide reportedly stimulates the central nervous system-mediated brown adipose tissue thermogenesis and adipocyte browning independent of food intake in mice . Because this profile and potential mechanisms of weight loss induced by liraglutide are different between rats in the Anderberg study  and mice in the present study, it is not at all surprising that different brain factors are engaged by liraglutide in these two species. In addition, the effect of 5-HT2A agonist on feeding seems to be opposite between mice and rats, at least by comparing the studies. Moreover, our study demonstrated that liraglutide acutely decreased the expression of hypothalamic 5-HT2A receptors and food intake in mice.