(A) Reaction scheme. See Materials and methods for details. (B) Alkaline agarose gel of leading strand products synthesized by the core leading strand replisome with the indicated amounts of Mcm10. (C) Purified MTC complex is shown to the left. Numbers to the left of the SDS PAGE gel show the migration of molecular weight markers (kDa). Time courses, to the right, are the core leading strand replisome with or without Mcm10 and Mrc1-Tof1-Csm3 complex (MTC) as indicated above the gel.