iIFNAR−/− macrophages were seeded into white 96-well plates and infected with L. monocytogenes at an MOI of 10. Staurosporine (AG Scientific) (5 µM) was added to uninfected macrophages at 30 min postinfection to induce caspase-3/7 activation. At 6 h postinfection, caspase-3/7 activation was measured using a caspase-Glo 3/7 assay kit (Promega) according to the manufacturer’s instructions.