Tgm9 excises at a high rate with a germinal reversion frequency of about 6% per generation . Several mutant genes, unlinked to the W4 locus, were isolated by screening germinal revertants for morphological mutant phenotypes [20, 22, 23]. For example, male-sterility, female-sterility and root necrotic root mutants were identified by screening thousands of germinal revertants carrying purple flowers [24, 25]. The present study was undertaken to determine if Tgm9 is suitable for creating knockout mutants for conducting large-scale functional analyses in soybean.