6) Figure 2D. GINS subunits are notoriously difficult to stain. It is a pity that in the CMG-Mcm10 gel appears to be cut so that the GINS subunits are not visible. From looking at this gel alone one cannot rule out the possibility that Mcm10 and GINS compete for the same binding site on the MCM. In Figure 2—figure supplement 1 the MonoQ profile is shown where Sld5 is detected, however not Psf1-3. It is remarkable that the CMG-Mcm10 complex is stable at 300mM KCl concentration (indeed convincingly shown in the high salt FLAG pulldown experiment), however the Q column experiment alone does not rule out the possibility that Sld5 and MCM-Cdc45-Mcm10 simply elute at the same salt concentration from an ion exchange column. Could the authors produce a gel where all of the CMG components are seen together with Mcm10, after FLAG elution?