This was hospital based cross sectional study involving HIV positive pregnant women in Mwanza urban conducted from October 2013 through January 2014. Mwanza urban has a population of 706,453 people where 237,478 are women in reproductive age group of 15 to 49 years [17, 18]. PMTCT services in the region started in 2004 at Nyamagana hospital and currently there are 45 health facilities which provide PMTCT services in the region. Ten out of 45 Health facilities in Mwanza urban were selected by simple random sampling using lottery method. Proportional to size sampling technique was used to obtain the number of study participants from each participating health facility (The size of each facility was the number of PMTCT attendees as per quarter of July-September 2013). We calculated the sample size based on the estimation of male involvement (defined as men who tested for HIV with their spouse at ANC) in the population of 21% , an absolute precision of 6%, design effect of 1.5  and a 5% level of significance. We adjusted the sample size by considering 10% of anticipated non response. Therefore the total sample size was estimated at 300 HIV positive pregnant women. All pregnant mothers with at least 18 years who tested for HIV and agreed to be enrolled into PMTCT program were included. A pregnant mother in first or second trimester or if her partner is not alive was excluded from the study. Eligible clients from each Health facility who attended on days of interview were enrolled sequentially into the study. A face to face interview was conducted using a pre tested, semi structured questionnaire to collect information on socio demographic information both for participants and their male partners, partner testing and providing support to the mother and Factors affecting male participation in various PMTCT services as perceived by the mothers. Information on mother disclosure of HIV test result to her partner and any negative consequences after disclosure was also obtained.