For AHO, functional assessment is equal to assessing the flow itself. This can be determined experimentally with outflow facility measurements in enucleated eyes or calculated from clinically measured variables with help from the Goldmann equation . Simultaneously, another functional assessment of AHO is to actually visualize where and how aqueous humor flows in the eye. This is important as, instead of considering the eye as one single unit of outflow, regional variations can be tested and visually appreciated. Imaging of AHO can be divided into non-real time and real time methods.