The objectives of this analysis were, first, to characterize and assess three maize GM hybrids—MON-89Ø34-3 × MON-88Ø17-3 (three stacked IR traits plus a single HT trait), MON-89Ø34-3 × MON-ØØ6Ø3-6 (two stacked IR traits plus a single HT trait), and MON-ØØ6Ø3-6 (single HT trait)—grown at several sites in Mexico for evidence of biologically meaningful agronomic and phenotypic differences or adverse ecological effects due to the introgression of IR and/or HT biotech traits (ERA). The second objective was to confirm biological efficacy in terms of plant response of MON-89Ø34-3 × MON-88Ø17-3 and MON-89Ø34-3 × MON-ØØ6Ø3-6 maize hybrids against lepidopteran and coleopteran insect pests, and to assess tolerance to glyphosate-based herbicides and efficacy of weed management programs by all three maize hybrids.