Our analysis also confirmed the differential expression (N × T: log2 fold change −2.42; adj. p-value 3.96E-11) of the previously reported lncRNA ENSG00000271086 (NAMA), which is downregulated in PTC compared to normal tissues39, 41 and in BRAFV600E tumors compared to wild type tumors39 (Fig. 5B). NAMA is induced by inhibition of the MAPK pathway, growth arrest and DNA damage41 and our analysis also demonstrated that NAMA is downregulated in BRAFV600E patients (WT × BRAFV600E log2 fold change −1.66; adj. p-value 2.02E-15). All these independently validated lncRNAs demonstrate the reliability of our study (Fig. 5).