RPW8-NLR genes expansion and comparative analysis. (A) Evolutionary history of RPW8-NLR proteins annotated in Cucurbita pepo. Full NB domains (PF00931) of 26 RPW8-NLR proteins were used together with 3 well characterized reference genes (short gene name: ADR1, NRG1, and APAF1) to do a maximum likelihood analysis based on the Jones et al. w/freq. model. Model with the lowest BIC score (Bayesian Information Criterion) was considered to describe the substitution pattern the best. Sequence similarities were determined performing a ClustalW (default settings) multiple alignment. Evolutionary analyses were conducted in MEGA7. Labels show the bootstrap values higher than 50 (out of 100), are indicated above the branches. The tree is drawn to scale, with branch lengths proportional to the number of substitutions per site. Species to which belong sequences are indicated by colored spots. (B) Venn diagram of genes (Gs) clustered into orthogroups (OGs). Five species (Cucurbita pepo, Citrullus lanatus, Cucumis melo, Cucumis sativus, and A. thaliana) were used to generate the Venn diagram. In the graph were reported the number of specie-specific and common OGs.