The GenMark RVP panel was used according to instructions from the manufacturer. The extracted nucleic acid is reverse transcribed and amplified using viral specific primers with RT-PCR enzyme mix. The amplified DNA is converted to single-stranded DNA via exonuclease digestion and is then combined with a signal buffer containing ferrocene-labeled signal probes that are specific for the different viral targets . A visual description is available at https://www.genmarkdx.com/solutions/technology/esensor/. Amplicon detection is measured by the peak height of a current flowing between the gold electrode and the ferrocene labeled probe, which is brought into proximity with the electrode by the capture probe binding to the target amplicon. Thus the peak current flow in nanoAMPs is a function of the number of targets bound to the capture probes and the tightness of this bond. The technology is sufficiently sensitive to be able to detect single basepair(bp) mutations in cystic fibrosis (https://genmarkdx.com/solutions/panels/xt-8-panels/cystic-fibrosis-genotyping-test/) and in thrombophilia (https://genmarkdx.com/solutions/panels/xt-8-panels/thrombophilia-risk-test/).