Cells at a density of 2.5 × 104 cells/cm2 were seeded on appropriate supports for analysis 16 hrs before performing the experiments (time 0). The following experimental culture groups were established: untreated control groups (CTRL Normoxia) and hypoxic groups (CTRL Hypoxia) at 0, 3, 8 and 24 hrs. Each group was grown in normal culture conditions for 16 hr, after which they were subjected to hypoxia (1% O2) for 3 hrs, followed by reoxygenation to 24 hrs. All cultures were grown with DMEM‐F12 (89%), FBS (10%) and P/S (1%).