We next examined the potency of miR-128 at regulating Tert protein levels in HeLa cells. We determined that miR-128 significantly decreased Tert protein levels, compared to miR controls. In contrast, anti-miR-128 significantly enhanced Tert protein levels, relative to cells expressing miR control (Figure 2B). In addition, analysis of Tert protein levels and localization was analyzed by confocal analysis, demonstrating that miR-128 significantly reduces nuclear staining of Tert protein in HeLa cells (seen as red single channel Tert staining and pink staining in overlay images with DAPI stained nuclei), relative to miR control HeLa cells (Figure 2C). As expected, anti-miR-128 significantly enhanced Tert protein nuclear expression, compared to miR control HeLa cells (Figure 2C). Finally, we verified that miR-128 significantly regulates Tert protein levels in a small panel of cancer cell lines, including lung cancer (A549), colon cancer (SW620) and pancreatic cancer (PANC1), demonstrating that the effect of miR-128 on Tert is not limited to HeLa cells (Figure 2D). Taken together this data demonstrate that miR-128 regulates Tert expression both at the mRNA and protein levels in different cell types.