Viral protein detection by western blotting - miRNA-transfected and DENV-2-infected cells were detached with trypsin and lysed using Lysis solution (Applied Biosystems). Total proteins were quantified using the BCA Protein Assay (Pierce, Thermo Scientific, NH, USA). Total protein (50 µg) was loaded onto an SDS-PAGE gel and then transferred to a nitrocellulose membrane after electrophoresis. A primary mouse monoclonal antibody against the DENV NS1 protein (Thermo Scientific) and a secondary anti-mouse IgG antibody conjugated with horseradish peroxidase (HRP; Santa Cruz Biotechnology, USA) were used for detection. Finally, the signals were detected using the chemiluminescence ECLTM detection system (Pierce). Band intensities were quantified by densitometry using the image processing software ImageJ 1.49, freely available online.