The six DEGs were selected to verify the results of the RNA-Seq analysis by qRT-PCR using different RNAs from those used for RNA-Seq (Table 2). The results showed that the expression patterns of IAG, IR, OA receptor, Tra-2, 5-HT receptor 1, and FTZ-F1 all agreed well between RNA-Seq and qRT-PCR, which confirmed the data obtained from high-throughput sequencing (Figure 3).