After the lentivirus-containing Lenti-Con (lentivirus-carrying vectors) and Lenti-GSN (recombinant lentivirus-carrying GSN cDNA) vectors were transfected into natural killer (YTS) cells, green fluorescence was obvious in the infected YTS cells, as observed under a fluorescence microscope, and the result indicated a successful transfection (Fig. 1A). Flow cytometry analysis showed that the transfection ratio in cells was 70-80% (Fig. 1B). Real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis and western blot analysis demonstrated that the mRNA and protein levels of GSN were both significantly increased in the YTS cells transfected with the pCDH-CMV-MCS-EF1-copGFP-GSN vector (YTS-GSN cells), when compared with the YTS cells transfected with the pCDH-CMV-MCS-EF1-copGFP vector (YTS-Con cells) (Fig. 1C,D). Fig. 1.Transfection ratio of the Lenti-virus-containing Lenti-GSN vector and GSN overexpression in YTS cells. (A) Green fluorescence was observed in the transfected YTS cells under a fluorescence microscope (×200 magnification) at 48 h, which indicated a successful transfection. (B) Flow cytometric analysis demonstrated that the transfection ratio in cells was 70-80% at 48 h. (C) qRT-PCR analysis exhibited that GSN mRNA expression was higher in YTS-GSN cells than in YTS-Con cells at 48 h. (D) Western blot analysis showed that the level of GSN protein was higher in YTS-GSN cells than in YTS-Con cells at 48 h. GSN, gelsolin; YTS cells, nontransfected cells; YTS-Con cells, YTS cells transfected with the pCDH-CMV-MCS-EF1-copGFP vector; YTS-GSN cells, YTS cells transfected with the pCDH-CMV-MCS-EF1-copGFP-GSN vector. **P<0.01, ***P<0.001.