Juglone showed the highest inhibitory effect on the mutants and wild strain. In addition, we used JA as an inhibitor to investigate the change in the structure of juglone through modification of its hydroxyl group in order to increase the hydrophobicity. The decision to produce JA was partly due to the predicted activity of this derivative through the docking scores, which had a higher absolute energy of interaction than juglone. Overall, MIC test of JA demonstrated different results for the mutant and wild strains. Although docking showed a high potential for JA, the MIC test indicated slight decrease in activity, which might be due to lipophilic binding of JA to other cell components.