A = Vv/2Qa µm2where Vv (volume density of the immunomarker) was estimated by counting the points (36-point test).Vv = Pp/Ptwhere Pp is the number of points that touch the immunomarkers, and Pt is the total number of points in the test area of 200 µm2, which in our case was 36 points.Qa (number density of nuclei area) was calculated by the formulaQa = N/Atwhere N is the number of nuclei counted in the test area and At is our test area, which was 200 µm2.