Likewise, there is no obvious density that connects D168 and K271 (Figure 5 instead shows connecting density between β-strands that is expected at resolutions lower than 4.7A). The resolution in this area is again ~6-7Å according to the local resolution estimation. The authors should be more cautious and can suggest that a salt bridge may exist as evident by the point mutant D168A, which has an 80% reduction in hemolytic activity.