Separation of lymphocytes and granulocytes from whole blood. (A–C) Deformability profiles of lymphocytes and granulocytes enriched from blood of three different donors. (D–F) Relative abundance of lymphocytes and granulocytes in each outlet. (G,H) Images of the immunostained lymphocytes (green) in O6 and granulocytes (red) in O8. (I) Comparison of distributions of leukocytes in O4–9 of three different samples: (1) whole blood without stain, (2) with Hoechst 33342 and (3) with anti-CD markers conjugated with fluorescence tags.