The Newbler filtered assembly resulted in 10,690 contigs for the BBD-resistant data, 7630 contigs for the BBD-susceptible data, and 16,285 contigs for all libraries combined. The Newbler assemblies incorporated 84%, 81% and 86% of the sequence reads from the BBD-resistant, BBD-susceptible and the combined data, respectively. The longest transcripts obtained by Newbler in the resistant, susceptible and combined assemblies were 4651, 4336 and 10,681 bases respectively. The average length of the Newbler contigs across the three assemblies was 679 bases. The detailed Newbler assembly statistics are shown in Table 3. The “large contigs” from the Newbler assembly are those with 500 bases or longer, which overall averaged 949 bases in length.Table 3 F. grandifolia transcriptome sequence assembly summary obtained from NewblerAssembled Contig SequencesBeech “Resistant” LibraryBeech “Non-Resistant” LibraryBeech “Combined” LibraryAligned Bases (%)157,365,926(83.63%)143,162,558(80.91%)314,816,063(86.23%)Aligned Reads (%)438,153(82.04%)412,164(78.90%)893,216(84.54%)Number of Contigs, All10,690763016,285Total Contig Bases7,845,7004,478,07811,664,012Average Contig Length734587716Number of Large Contigs714740189943Average Large Contig Size9618751009N50 Large Contig Size10058931081Largest Contig Size4651433610,681