As we revealed earlier TPPP/p25 co-localizes with the MT network and displays extensive bundling activity in different cell lines expressed by transfection (HeLa) or doxycycline induction (CHO10) or endogenously (CG-4), and it regulates the dynamics and organization of the MT network via its tubulin polymerization promoting and MT bundling activity as well as tubulin acetylation enhancing activity5710. We have also shown with immunofluorescence microscopy that in contrast to the FL TPPP/p25 the CORE segment (double truncated form) distributed homogeneously within the cytoplasm5. Most importantly, we suggested that “The finding that the double truncated TPPP/p25 cannot associate with the MT network underlines the role of the unstructured termini in the physiological function of TPPP/p25” 5.