The ability of Wa1F1-KT to hydrolyze the soluble β-1,3-glucan laminarin (Sigma-Aldrich, St. Louis, MO, USA) was assayed in comparison to Wa96603-KT, Wm41968-KT (with recognized β-glucanase activity ), and laminarinase (Sigma-Aldrich). The reaction mixtures contained 20 µL of 50-fold concentrated crude KTs or 10 µL of laminarinase (4.5 U/mL) and 2 mg/mL laminarin in 100 µL of citric acid-Na2HPO4 buffer (0.01 M, pH 4.5). After incubation at 25 °C for 2 and 4 h, the reaction was stopped by heating at 100 °C for 15 min. The activity on laminarin was estimated through observation of the end products of laminarin hydrolysis by thin layer chromatography . Reaction mixtures containing crude KTs inactivated by heating at 100 °C for 15 min were used as controls.