This was performed with DAPI as well as H&E which are shown in Figs 2 and 3 respectively. Collagen was also assessed with masons trichrome. Cell quantification was conducted using imageJ software with intact nuclei in each image being counted using the cell counter plugin for the DAPI and H&E slides.Figure 3DAPI (4′,6′-diamidino-2-phenylindole) analysis of cartilage specimens assessing for decellularization with quantification of nuclei count per slide (n = 3) for different protocols using imageJ. Error bars for standard deviation.