Antibodies directed against AMPK-α, AMPK-α-phospho-Thr172, and ACC1-phospho-Ser79 (acetyl coenzyme A) were all obtained from Cell Signaling Technology (#2532, 2531, and 3661, Danvers, MA) and applied in dilution 1:1,000 as previously described (Li et al., 2015; Al-Bataineh et al., 2016). Anti-β-actin antibody was obtained from Sigma (A1978, St. Louis, MO; Pastor-Soler et al., 2015).