We demonstrated that average number of H3K27me3-enriched genes was higher in tumor tissues than normal tissues. Then, after factorial discriminant analysis and ANOVA, we characterized the significant interaction of H3K27me3 with ALG5, EXOSC8, CBX1, GRID2, GRIN3B, ING3, MYO1D, NPHP3-AS1, MSH6, FBXO11, SND1, SPATS2, TENM4 and TRA2A in tumor tissues compared to normal tissues. These genes were all more H3K27me3-enriched and were able to discriminate different groups according to Gleason score.