The selection of the 10 best ISSR primers for genetic studies in A. flaccida was performed as follows: first, the 10 primers that showed the highest values for each attribute (PIC, MI, and RP) were identified separately. Then, 10 ISSR primers were identified according the following criteria in the following order: 1. primers that showed the highest values for PIC, MI, and RP, 2. primers that showed the highest values for PIC and PR, 3. primers that showed the highest values for MI and RP. Whenever a primer was selected, it was excluded before applying the next criteria.