The study concludes that splash of BBFs on intact skin was the most commonly reported exposure among HCWs in this tertiary hospital. HCWs in surgical departments reported they were least protected. Majority of HCWs had positive attitude towards PLWHA. However, a large proportion of nurses believed that all patients should be routinely screened for HIV. Half of HCWs had accessed HCT service. There is need to ensure that workers are well protected in the workplace. All aspects of Standard Precautions should be employed in all patient care whether the patient is HIV positive or not and protective equipment required to practice Standard Precaution should always be available to reduce the risk of exposure to BBFs. The University College Hospital workplace policy on HIV/AIDS should be made available to HCWs so they can be aware of what is expected of them in their care of PLWHA. HCWs should be encouraged to go for HCT in order to be effective role models in the prevention of HIV/AIDS.