rCsGRN was probed with mouse anti-His tag serum, rat anti-CsGRN serum, rat anti-CsESPs serum, and rat naïve serum as a control (Fig. 2b, Lanes 1–4). In addition, CsESPs was probed with rat anti-CsGRN serum (Lane 5) and rat naïve serum as a control (Lane 6). Rat anti-CsGRN serum could specifically recognise CsESPs, and rat anti-CsESPs serum could react with rCsGRN (Fig. 2b, Lanes 2, 5), confirming that CsGRN was an excretory-secretory product of C. sinensis.