Given that KDM2B possesses two different isoforms , we investigated the possibility that our mRNA analysis was only measuring one specific isoform, not recognised by the antibodies we have tested. As such, we designed specific PCR primers directed at each of the KDM2B isoforms and repeated our qPCR analysis in cells exposed or not to hypoxia. This analysis revealed that hypoxia induces the expression of both KDM2B transcripts significantly in both HeLa and U2OS (Figure 2C). This confirmed our initial mRNA analysis for total KDM2B mRNA, and suggests that hypoxia induces changes in KDM2B protein at the post-transcriptional level.