(A) miR-modulated HeLa cell lines were generated (over-expressing miR-128, anti-miR-128 or control miR). Relative expression levels of Tert RNA were determined and normalized to beta-2-microglobulin (B2M) expression levels. (B) Stably miR-128, anti-miR-128 or control miR control HeLa cells lines were analyzed by western blot analysis using antibodies against Tert and α-tubulin. One representative example of three is shown. Quantification of results (n = 3) normalized to α-tubulin is shown (right panel). (C) Stable miR-128, anti-miR-128 and control miR HeLa cell lines were analyzed by immunofluorescence for Tert expression and co-localization with DAPI. Quantification of results is shown (bottom panel) (n = 3). (D) Stable A549, SW620 and PANC1 miR-modulated (miR-128, anti-miR-128 and control miR) cell lines were generated and Western blot analysis were performed using Tert and α-tubulin antibodies. Quantification of results (n = 3) normalized to α-tubulin is shown (bottom panels). All results are shown as mean ± SEM, n = 3, independent biological replicates. *p < 0.05, **p < 0.01, ***p < 0.001, by two-tailed Student’s t test.