Taken together, non-real time imaging techniques have emphasized the segmental nature of physiologic AHO and a potential relevance for treating ocular hypertension. Particularly exciting is the link of the segmental patterns to possible protein differences that might subserve the segmentalization. Note though that emphasis was on the TM here. Some of this was due to the use of larger particles. Larger particles allowed better maintained intraluminal presence without leakage so that tracers stayed within the AHO pathways during the processing time necessary for the non-real time assessment. However, larger particles may have also had a harder time passing through the TM easily. Thus, while clearly important for understanding AHO biology in model systems, non-real-time approaches using tagged beads or tracers may have a more limited clinical use in live human subjects and patient care.