Semi-structured, self-administered questionnaires developed from the reviewed literature were used to collect information on socio-demographic characteristics, occupational characteristics, risk of exposure to HIV, attitude of HCWs to PLWHA, willingness to participate in HCT, factors affecting HCT uptake, and barriers to HCT uptake. The questionnaire was pretested among HCWs in Ladoke Akintola Teaching Hospital, another teaching hospital in Southwest Nigeria. Ethical approval to carry out the study was obtained from the Joint University of Ibadan and University College Hospital Institutional Review Committee and informed consent was obtained from respondents.