Considering the contradictory role of CBX7 in different tumors, the transcription level of CBX7 in four glioma databases (CGGA, TCGA, REMBRANDT and GSE16011) was analyzed. We found that CBX7 mRNA levels in high-grade gliomas (HGG) were significantly decreased compared to normal brain tissues (NBT) and low-grade gliomas (LGG). CBX7 expression was also decreased in LGG in comparison to NBT (Figure 1A). Further, the association between CBX7 mRNA levels and clinical progression of glioma patients was determined using overall survival rates in GBM patients (samples from TCGA) and HGG patients (samples from CGGA, REMBRANDT and GSE16011), and Kaplan-Maier analysis and log-rank comparison were performed. In TCGA database, there was no significant difference in clinical outcome between the high and low CBX7-expression groups (high versus low median survival, 418 versus 375 d, respectively; p = 0.4922). However, in the three other databases, decreased CBX7 expression was associated with a shorter survival period (Figure 1B). Twenty human glioma specimens were divided into LGG or HGG groups and four brain samples obtained from epilepsy surgery were used for control group. In these samples we found that protein and mRNA levels of CBX7 are also negatively associated with tumor grade (Figure 1C and 1D). We also found that CBX7 expression possesses subtype preferences within gliomas (Supplementary Figure 1). These multi-center data and tumor samples revealed that CBX7 is a potential prognostic factor in glioma patients.