Of the 42 ISSR primers evaluated in A. flaccida, 35 primers (83.3%) showed DNA amplification (Table 3). The total number of amplified fragments was 375 with a mean of 10.7 fragments per primer. The PIC, MI, and RP values for each primer are shown in Table 3. Based on the PIC, MI, and RP data, the primers identified as more informative for genetic studies in A. flaccida were UBC 890, UBC 834, UBC 866, UBC 808, UBC 827, UBC 807, UBC 857, UBC 848, UBC 855, and UBC 835 (Table 3).