The proteome functional annotation was performed through a match search against four database (TAIR10, SWISS-PROT, TrEMBL and GenBank-NR) using DIAMOND in sensitive mode with a cut-off e-value of 1 e−5 (Buchfink et al., 2015). To add information about protein function to our proteome, a Blast2GO (Conesa et al., 2005) annotation, using default parameters, were conducted. Finally, the zucchini proteome was scanned with InterProScan v.5.13 (Jones et al., 2014) against the InterPro protein signature databases to identify and finely characterize plant resistance proteins.