CaLC3672/CaLC3673: To introduce a mutant allele of CAS5 carrying S462A, S464A, S472A, and S476A mutations, the CAS5S 462A/S464A/S472A/S476A -HA-HIS1 cassette was released from pLC858 with SacII and transformed into CaLC2034. Transformants prototrophic for histidine were PCR tested as described for CaLC3693. The absence of an untagged wildtype CAS5 allele was verified with oLC3052 in combination with oLC2164. The S462A/S464A/S472A/S476A mutations were sequence verified with oLC3371.