Reports from Western countries have shown coagulase negative Staphylococcus as the most common organism in postoperative endophthalmitis.[1–3,5,6,9,13,22] Gram-negative bacilli were the most common group isolated in our study. Pseudomonas was the most common organism cultured. Our finding is in agreement with previous reports from Indian subcontinent. Sharma et al. have reported a high prevalence of gram-negative bacteria and fungus in postoperative endophthalmitis. Anand et al. and Jambulingam et al. have reported similar spectrum in postoperative endophthalmitis from our center. A similar high prevalence of gram-negative bacteria has been reported in other infections in subjects with diabetes. We believe both varied geographical distribution and predominance of subjects with diabetes in our study are responsible for this microbial spectrum.