Delayed-type hypersensitivity (DTH), as the fourth type of hypersensitivity reaction, is an important type of cell-mediated pathologic response, and plays a pivotal role in evaluating T cell-mediated immune responses . In this research, SRBC were used to induce footpad DTH reaction. We found that AHG potentiated the SRBC-induced DTH reaction in footpads of CY-treated mice and counteracted the inhibitory effect of CY on the DTH reaction. The foot volume was increased after AHG treatment, suggesting the cell-mediated immune in CY-treated mice was enhanced.