Calpastatin overexpression protects against skin aging. At 2 years, CalpTG (TG) mice (B) were phenotypically less impacted by aging than WT mice bred in the same conditions (A). There was a macroscopic evidence that CalpTG (TG) mice fur was less affected by aging than WT mice. In addition, we observed less lipofuscin deposits in the skin after hematoxylin eosin staining (C,D, white arrow, magnification ×200) or by using lipofuscine autofluorescent properties (E,F, white arrow, magnification ×200). Moreover, telomere lenght decreased significantly in WT mice skin but not in CalpTG skin (G, **p < 0.01, n = 10/group).