All experiments were performed in triplicate and repeated three times. Analysis of variance (ANOVA) and the Student's t-test were employed to analyze the difference between the groups for statistical significance. P < 0.05 was considered statistically significant. The data is represented as the mean ± standard deviation. The asterisk in the bar graph denote statistical significance. The significance level is represented in the graphs as ***P < 0.001, **P < 0.01, *P < 0.05, NS-non significant.