A549, H1975 and PC9 were chosen to represent the typical NSCLC cell line in this study. Function analysis was conducted in vitro cell line to study the effects of PARP1 on NSCLC invasion by adopting overexpression and inhibition of PARP1 expression. The overexpression and silence cell line of PARP1 gene with NSCLC cell line A549, H1975 and PC9 was established, then the PARP1 expression was detected in the 3 cell lines.