Nanoparticle delivery systems, including viral and non-viral ones, have been addressed to protect miRNAs from degradation by serum nucleases when administered systemically . The advantage of non-viral approach is safety and avoiding induction of toxic immune response compared with viral delivery. Non-viral delivery systems mainly use liposomes, lipoplexes and polyplexes as miRNA carriers. The encapsulation of miRNAs into polycation liposomes has been well studied. Muramatsu et al. injected miR-125b directly into the subcutaneous tumor using non-viral vectors composed of the cationic polymer polyethylenimine (PEI). Administration of miR-125b induced formation of non-functional blood vessels and inhibited in vivo tumor growth by targeting VE-cadherin, suggestive of therapeutic potential . Another kind of liposome is neutral liposomalparticle 1,2-dioleoyl- sn-glycero-3-phosphatidylcholine (DOPC) . Wu et al. reported that the delivery of miR-192, which targets EGR1 and HOXB9, to tumors using the DOPC nanoliposomes significantly inhibited tumor angiogenesis of multiple ovarian and renal cancer models . This delivery platform has also been used for miR-200 and miR-520d in several cancer types in preclinical models to inhibit tumor angiogenesis in vivo [117, 118].