In the experiment comparing AAV-WT and AAV-2P, the amount of soluble human tau present in forebrain homogenates was similar in these two groups of mice, at 7 months after vector injection (Fig. 3c). Moreover, a sarkosyl extraction demonstrated similar levels of human tau in both the sarkosyl-soluble and insoluble fractions, indicating that the 2P modification did not have any effect neither on the overall tau expression nor on the deposition of insoluble human tau (Fig. 3c).