CaLC3113: To C terminally HA tag CAS5 in a mutant heterozygous for CAS5, the HA-HIS1 cassette was PCR amplified from pLC57562 using primers oLC2161 in combination with oLC2162 and transformed into CaLC2034. Transformants prototrophic for histidine were PCR tested with C2163 in combination with oLC2029 for upstream integration and oLC2164 in combination with oLC1645 for downstream integration.