Recently, we have developed a novel strategy for the construction of supramolecular assemblies by means of host induced aggregating (HIA: a small amount of macrocyclic host could promote the aggregation of guest molecules by lowering the critical aggregating concentration (CAC) and even regulating the morphology of the aggregates)5051525354555657. So after successful establishing the recognition motif between 2C-WP5A and G in aqueous solution, we were trying to explore the constructing supramolecular aggregates based on 2C-WP5A and trans-G. G could be induced by 2C-WP5A to form large assembly via HIA by monitoring the dependence of the optical transmittance at 600 nm on the concentration of G (There is no absorbance of G at 600 nm Fig. 4a). It is noteworthy that in the absence of 2C-WP5A, the optical transmittance of trans-G at 600 nm showed no appreciable change as the concentration increased form 0.8 mM to 2.0 mM (see Supplementary Fig. S18). It is requisite to find the best molar ratio between 2C-WP5A and G for fabricating the supramolecular assembly. As shown in Fig. 4b, the optical transmittance at 600 nm of the trans-G solution first decreased sharply upon the gradually added 2C-WP5A until reaching the minimum and then gradually increased as the amount of 2C-WP5A continued to increase. By monitoring the change of the transmittance at the wavelength of 600 nm in the process, the best molar ratio of trans-G and 2C-WP5A can be determined at 1:0.06. Redundant amount of 2C-WP5A leads to the formation of a simple inclusion complex and disaggregation of the aggregates, accompanied with the rising of the optical transmittance. This phenomenon highlighted the important role of HIA in the formation of the supramolecular assembly. In fact, only a small amount of macrocyclic host (6%) could promote the aggregation of guest molecules. In order to prove the prominent effect of the macrocyclic structure of host, a control experiment showed that no assembly appeared when replacing 2C-WP5A with its fragment benzene-1,4-dioxyacetic acid ammonium salt (DAAS) under comparable conditions (see Supplementary Fig. S19), indicating that the cyclic structure of 2C-WP5A is vital in inducing the supramolecular assembly. Furthermore, in order to make comparison with HIA, we fixed the concentration of 2C-WP5A at 1.6 mM, gradually added trans-G and controlled the experiment procedure same to the HIA. As shown in Fig. 4c,d, the transmittance the 2C-WP5A is not changed upon the addition of G from 0 to 1.0 eq. So for induced aggregating, the poly-charged additive is necessary such as the macrocyclic host molecules. Overall, there are three main points in HIA: (1) strong binding affinity between host and guest; (2) charge interaction between host and guest; (3) the macrocyclic structure of host molecule.