EGFR is widely expressed in mammalian epithelial cells. Previous studies have demonstrated higher EGFR  and EGF expression in pancreatic cancer tissue compared to normal pancreatic tissue. The expression of EGF/EGFR was correlated with tumor proliferation, invasion, and metastasis . EGFR inhibitors can inhibit the growth and proliferation of tumor cells . Our results indicated that PA-MSHA treatment did not affect the total levels of EGFR signaling pathway components compared to controls. However, the levels of pEGFR, pAKT, and pERK decreased in response to treatment with increasing concentrations of PA-MSHA, suggesting that PA-MSHA inhibited phosphorylation of these key downstream signaling proteins. Thus, PA-MSHA inhibits EGFR signaling and cell proliferation in pancreatic cancer cells.