(A) Human PBMCs, (B) murine BMDCs, and (C) murine peritoneal macrophages (pMAC) were transduced with control or mixed DHX29 sgRNA/Cas9 lentiCRISPR viruses. Transduced cells were selected by puromycin treatment for 48 h to eliminate untransduced cells. Puromycin-resistant cells were cultured with fresh medium, left untreated or stimulated with HMW Poly(I:C) or Poly(dA:dT) in lyo/vec form (Vec) or infected with EMCV or VSV at an MOI of 10. Phosphorylation of IRF3 and IFN-β protein were determined by western blot and ELISA.