EVs present in whole plasma or PF were characterized using Nanoparticle Tracking Analysis (NTA). One microliter of sample was diluted in sterile water to obtain a suitable concentration to be read, according to the manufacturer’s guidelines. The sample was passed through the flow cell. Once the temperature of the flow cell had stabilized at 25°C, six 30 s videos of each sample were taken with a high-resolution camera, with a 1 s pause between each. The videos were then processed by Nanoparticle Tracking Analysis software (version 2.3), giving the concentration of particles per ml for each nanometer size. The final data for each sample is an average of the data from six videos.