Intracellular replication of bacteriolysis in different cell types. (A) Bacteriolysis mutants (MOI of 0.2) were examined for intracellular replication in BMDMs. This panel depicts the data from the experiment in Fig. 2A but displayed as CFU at 0.5, 2, 5, and 8 h postinfection. (B) ΔmenD and ΔmenF strains were grown in minimal media plus 25 ng/ml MK overnight. Infected BMDMs at a starting MOI of 0.2 were then enumerated for CFU at 2 and 5 h postinfection. MK (50 μg/ml) was added exogenously to infected cells to complement growth of MK-deficient strains. Data are calculated as fold change between 2 and 5 h and representative of results from three independent experiments. (C to E) Bacteriolysis mutants were grown in primary IFNAR−/− macrophages (MOI of 0.2) (C), Caco-2 cells (MOI of 5) (D), and BHKs (MOI of 5) (E) and then enumerated for CFU at 2 and 5 h postinfection. Data are calculated as fold change between 2 and 5 h and representative of results of two independent experiments. (B to E) The panels on the right depict the same data but graphed as CFU at 0.5, 2, 5, and 8 h postinfection. Download FIG S4, TIF file, 1.8 MB.