Potential mechanisms regulating the rate of lipid export from the intestine beyond lipoprotein levels, the regulation and physiological effects of the size of enterocyte lipid droplets, and the channeling of newly absorbed dietary fatty acids into the different classes of complex lipids are currently largely uncharacterized. The optically clear and genetically tractable larval zebrafish model presents an ideal system in which to investigate these questions relating to energy homeostasis with a combined live imaging and biochemical approach.