IVY1 and ATG5 suppress the GFP-Atg8 processing defect at 37° in the ypt6Δ strain. Cells were grown then starved for nitrogen as described. (A) Fluorescence images of all strains in nonstarvation conditions. (B) Fluorescence images of all strains in starvation conditions at 30°. (C) Fluorescence images of all strains in starvation conditions at 37°. (D) The percentage of the cells with the green diffusion phenotypes in starvation condition at 30 and 37°. At least 100 cells were counted for each strain. Error bars represent SD from three biological replicates. Scale bar: 3 μm. EV, empty vector.