For the detection of various forms of tau, the following primary antibodies were used: HT7 human specific anti-tau (Thermo Fisher Scientific, MN1000), anti-phosphorylated tau AT8 (pSer202 and pThr205, Thermo Fisher Scientific, MN1020) and PHF1 (pSer396 and pSer404, kindly provided by Dr Peter Davies), anti-PHF tau MC1 (kindly provided by Dr Peter Davies) and AT100 (Thermo Fisher Scientific, MN1060).