Telomere‐specific FISH in HCC tissues. (A) Left: representative image of telomere‐specific FISH on whole slides to elucidate the sub‐location and micro‐anatomic distribution of telomere signals (original magnification ×40). Right: the same section is divided into 48 grids, colored according to the relative telomere density. (B) Top: representative H&E staining of HCC with (left) or without (right) a tumor capsule (original magnification ×20). Bottom: FISH staining in the case with a tumor capsule. White dotted lines highlight the margin area (original magnification ×40). (C) Telomere‐FISH intensity quantification (n = 30). Lines indicate the 25th, 50th, and 75th percentiles. ***p < 0.001. (D) Representative examples of telomere length variation in tumor cells and CAFs in TMA. (a) Strong telomere signals in cancer cells; (b) weak telomere signals in cancer cells; (c) extremely short telomeres in CAFs; (d) long telomeres in CAFs. Asterisks indicate cancer cells and arrows indicate CAFs (original magnification ×40).