Tasdemir, et al.27 (2011) evaluated the use of NaOCl and NaOCl+EDTA with different agitation techniques (CanalBrush, PUI, MAF) on the removal of CH. Although the type of solution did not influence the CH removal, CanalBrush and PUI were found statistically more effective than other techniques. Our results were not consistent with the findings of the aforementioned study. Although CanalBrush removed more CH than the needle groups, this difference was not statistically significant. Another difference is that the PUI removed significantly more CH than CanalBrush in all three root regions. Tasdemir, et al.27 (2011) used field measurement methods as opposed to our study that utilized a preferred scoring method with a PUI time interval of 1 min.