As a neural characteristic structure, the number of Nissl bodies reflects the state of neurons. In physiological conditions, the Nissl bodies were big and abundant, showing that the function of neuronal protein synthesis was strong; on the other hand, when nerve cells were damaged, the number of Nissl bodies will be reduced or even disappear. Intraneural Nissl bodies of the cortex and hippocampus were lightly stained and appeared to be sparsely arranged in the model group. However, deeper stained Nissl bodies with higher density in cortex and hippocampus neurons were found in the sham and DNLA groups (Fig. 5).