Our study demonstrates that 5′UTR remodeling by RBP is important for the transcript-specific translation regulation. The RNA binding is critical for exposing Sbp1's central RGG repeats, which in turn is important for the molecular interactions between Sbp1, Pab1, and eIF4G1. In contrast, deletion of the RRM domain, whereby leaving the RGG domain alone, regardless of its methylation state, inhibits the translation of all the examined RNAs including Pab1, eIF4E, and CrPV in both cap-dependent and cap-independent pathways (Fig. 6, columns 5 and 6).