The remaining mice received additional training every day for 9.5 days (Advanced Training). The final measurement of their performance occurred during the last phase (Data Acquisition). To test the behavioral memory, three mice were given a break for 22 days after their training and testing. During this break harnesses were removed and the mice were allowed free access to food. Prior to memory recall testing, the harness was once again placed on the mice and food restriction was reinitiated. Mice were then placed back on the VR setup with no additional training, and allowed to locate the same reward target as before.