When comparing WT and 2P tau, we did not find any significant effect of the 2P modification neither on the amount of misfolded tau in the mouse forebrain (HT7/MC1 AlphaLisa normalized to total human tau, Fig. 4i), nor on the amount of tau multimers (HT7/HT7 AlphaLisa normalized to total human tau, Fig. 4j), at 3 and 7 months post-injection.