The levels of DAO and D-lactate after ETEC challenge are shown in Figure 3. At 24 h after ETEC challenge, the DAO and D-lactate concentrations in the ETEC group were significantly higher (P < 0.05) than those in the CONT and MDLP groups but did not differ (P > 0.05) from those of the HDLP group. At 0 and 144 h after ETEC challenge, no difference (P > 0.05) in DAO and D-lactate concentrations was noted in all the groups.