The mRNA sequence annotated with granulin (CsGRN) was selected from our C. sinensis cDNA plasmid library and was identified by DNA sequencing. The domains, physicochemical properties and some structures of the translated amino acid sequence were predicted with proteomics tools at NCBI (http://www.ncbi.nlm.nih.gov) and ExPaSy website (http://www.expasy.org/), and the disulphide bonds were analysed through the website (http://scratch.proteomics.ics.uci.edu/). The multiple alignments of sequences with homologues from human and helminths were carried out by Vector NTI software, and the phylogenetic tree was constructed with corresponding sequences from 14 other species using the software MEGA version 6.0 .