Next, gels were washed with washing solution [10% methanol and 7% acetic acid] for 30 min. The gels were incubated in SYPRO Ruby stain (Life Technologies) for 90 min in the dark. The gels were washed with destaining solution [10% methanol and 7% acetic acid] for 30 min and rinsed with MilliQ H2O. Image acquisition was carried out using a Fluorophorestar 3000 image capture system with a 470 nm excitation and 618 nm emission filter for SYPRO Ruby detection.