Bacteriolysis mutants identified in the genetic screen undergo complete bacteriolysis, inside macrophages. (A) Genetic complements of clean deletions and transposon mutants (MOI of 10) were tested for bacteriolysis in iIFNAR−/− macrophages using the bacteriolysis assay. Data are normalized to wild-type levels of bacteriolysis and presented as means ± SEM of results from six independent experiments. NT, not tested. (B) Bacteriolysis mutants carrying chromosomal bacteriolysis reporter pYL56 (MOI of 10) were tested for bacteriolysis in iIFNAR−/− macrophages 6 h postinfection. Ampicillin (Ap; 1 mg/ml) was added to the wild-type infection as a positive control. All data are presented as mean fluorescence ± SEM from six independent experiments. Download FIG S2, EPS file, 1.2 MB.