n‐way Anova has been performed to determine the existences of interaction between n independent variables (time and treatment for two way anova and time, treatment and oxygen levels for three way Anova) on a continuous dependent variable. Tukey honest significant difference (HSD) has been used as post hoc test when Anova test indicated statistically significant differences to identify specific changes in time‐points or treatment conditions. Statistical calculation has been performed using R software (R Foundation for Statistical Computing). Values are represented in graphs as mean ± standard error of the mean (SEM). All calculation was performed using GraphPad Prism v7 software.