Supaglutide-treated mice were more tolerant with cold exposure. Mice after 4 weeks' treatment were starved for 6 h, followed by being placed for 6 h in a room with a temperature of 4–8°C. Body temperature was recorded once per hour with a rectal probe connected to a digital thermometer and the significant difference is indicated for control vs. SPG by two-way RM ANOVA. All values are means ± SE (n = 5 for each group). *p < 0.05.