SUB1 cDNA (Origene Technologies, Rockville, MD, USA; cat. no. RC204999; Myc-DDK tagged) was cloned into Gateway expression system (Life Technologies). To generate lentiviral constructs, PCR8-SUB1 (Myc-DDK tagged) was recombined with pLenti6/V5-Dest (Life Technologies) using LR Clonase II (Life Technologies). Lentiviruses were generated by the University of Michigan Vector Core. Benign immortalized prostate cells (RWPE) were infected with lentiviruses expressing SUB1 or lacZ, and stable clones were selected with 3.5 μg/ml blasticidin (Santa Cruz Biotechnology Inc., Dallas, TX, USA).