Average total daily time spent in sedentary, standing and stepping activity, in addition to total daily step counts and sit to stand transitions, were computed from the activPAL software for 1) weekdays and 2) weekend days. Paired t-tests were used to examine differences in total daily time spent in sedentary, standing and stepping activity, in addition to total daily step counts and sit to stand transitions between weekdays and weekend days. Participants were grouped according to time spent sitting based on the overall group mean. In relation to weekday sedentary behavior (n = 42), 10 participants were in group 1; 14 participants were in group 2 and 18 participants were in group 3. In relation to weekend sedentary behavior (n = 39), 13 participants were in group 1; 12 participants were in group 2 and 14 participants were in group 3. Differences between sitting (Group 1 = <8hrs/day, Group 2 = 8–10 hrs/day, Group 3 = >10hrs/day) and components of the HADS and SF12 were examined using an ANCOVA with a measure of physical activity (step count) included as a covariate. Partial eta-squared (ηp2) effect sizes were used to evaluate the strength of association for between group differences. Values of 0.01–0.03, 0.06–0.09 and >0.14 indicate a small, medium and large effect, respectively .