In accord with GLP-1's capability in regulating gastric empting and food intake, supaglutiude treated mice showed reduction in body weight which was associated with reduced food intake significantly (Figure 6A, SPG vs. Ctrl = 1.24 ± 0.00 vs. 2.00 ± 0.05 g/day, p < 0.01). To determine whether supaglutide affected energy expenditure, we measured metabolic rate by indirect calorimetry using Comprehensive Lab Animal Monitoring System (CLAMS). As shown in our study, VO2 (Figure 6B), VCO2 (Figure 6D), and energy expenditure (EE) (Figure 6F) expressed as per-whole-mouse showed a trend, but not statistically different, of increase in supaglutide group. When normalized by body weight, VO2, VCO2, and EE of the supaglutide-treated mice demonstrated significant increase in the dark cycle (Figures 6C,E,G).