MiR-19 antagomirs significantly inhibited KPC cell line invasion, but not migration, consistent with an invasion-specific effect for miR-19 family miRNAs (Figure 8A, 8B). This response inversely correlated with the level of endogenous miR-19 family expression, suggesting a dosage response (Figure 8C). Indeed, the cell line with the highest expression of miR-19, 9415#2, was resistant to antagomirs at a concentration of 50 nM, but responded when treated with antagomirs at 100 nM (Figure 8A, 8C). We next ascertained whether miR-19 regulates invasion in human PDAC cells. The human pancreatic cancer cell lines MIA Paca-2 and PANC-1 are also invasive and express relatively high levels of miR-19 (Figure 8F). Treatment of these lines with miR-19 antagomirs reduced their invasive capacity in a transwell assay without affecting their migration ability (Figure 8D, 8E). Furthermore, treatment with miR-19 antagomirs was sufficient to reduce the number of invadopodia rosettes formed in KPC cells (Figure 8G, Supplementary Figure 10) and also decreased the gelatin degradation capacity of these cell lines (Figure 8H, Supplementary Figure 11). These data demonstrate that miR-19 miRNAs regulate PDAC cell invasion.