HRESI(+)MS measurements confirmed that 1 (C7H8O6, Δmmu +0.2), 2 (C7H8O6, Δmmu +0.1) and 3 (C7H8O6, Δmmu +0.1) were isomeric, while analysis of the one-dimensional (1D) and two-dimensional (2D) NMR (methanol-d4) data (Figures S8–S13, Tables S2 and S3) suggested they were mono methyl esters of E-aconitic acid, for which we attribute the trivial names aconitates A–C. Assignment of E ∆3,4 configurations were inferred from diagnostic chemical shifts for H-4 and C-2 in 1 (δH 6.92; δC 33.8), 2 (δH 6.89; δC 33.9), and 3 (δH 6.91; δC 33.9), when compared to the authentic standards for E (δH 6.90; δC 33.8) and Z (δH 6.26; δC 40.2) aconitic acid (Figures S1–S7, Table S1). HMBC correlations permitted assignment of the methyl ester regiochemistry across 1–3 with correlations from (i) the OMe (δH 3.67) and H2-2 (δH 3.89) to C-1 (δC 172.7) confirming a C-1 CO2Me in aconitate A (1), (ii) the OMe (δH 3.81) and H2-2 (δH 3.89) to C-6 (δC 168.4) confirming a C-6 CO2Me in aconitate B (2), and (iii) the OMe (δH 3.77) and H-4 (δH 6.91) to C-5 (δC 167.5) confirming a C-5 CO2Me in aconitate C (3) (Figure 2).