RT-PCR: 0.3 µM of the HPLC purified primers, M-forward: 5′ ACGAAAGTCTGATGGAGCAATA 3′ and M-reverse: 5′ ACGCCCAATAAATCCGRATAAT 3′ . RT-PCR was performed using the Mx30000P® and Brilliant® SYBR Green QPCR master mix (Stratagene, California, USA) essentially as described by the manufacturer. The RT-PCR reaction was run under the following conditions: Segment 1: Initial denaturation 95 °C 10 min, Segment 2: 95 °C 30 s, 60 °C 1 min, 72 °C 30 s, for 40 cycles, Segment 3: 95 °C 1 min, ramp down to 55 °C and ramp up from 55 to 95 °C. Dissociation temperatures were 77.4 °C for CMhm, 78.0 °C for Mhf and 79.5 °C for CMt. Primers for both conventional and RT-PCR amplify products of 193 bp for CMhm and 170 bp for both Mhf and CMt.