In silico analysis showed that NnTPS genes exhibited differential abundance in various tissues and some of them are obviously regulated by environmental stimuli. Some of these obviously changed genes were then validated by RT-qPCR. As shown in Figure 8, RT-qPCR results of genes under various tissues and stresses were similar in magnitude to those obtained by deep sequencing. NNU_020889, NNU_014679, and NNU_022788 were the most abundant genes in rhizome. It is interesting, that while under submergence, NNU_020889 was significantly induced while NNU_000788 was significantly down-regulated. Moreover, NNU_024672, which has the minimum transcript level were further down-regulated upon submergence.