We first assessed the effect of HMW Poly(I:C) stimulation on DHX29 expression or induction, and found that DHX29 expression was not affected by HMW Poly(I:C) or EMCV stimulation (S2A and S2B Fig). We next performed knockdown (KD) experiments and observed that three different DHX29-specific lentivirus short hairpin RNA (shRNA) constructs could effectively knockdown endogenous DHX29 expression (S2C Fig). Furthermore, changes in cell viability were not observed for 3 days following transfection of shRNA targeting DHX29 (S2D Fig).