Completely different results were found in one CD5+ B lymphocyte subpopulations. Earlier, this subpopulation was thought to be a potential source of autoantibodies, but its role in autoimmunity is still being investigated. Elevated CD5+ expression on peripheral B lymphocytes correlated with MS disease activity expressed by a number of gadolinium-enhancing lesions on MRI and inversely correlated with disease duration  and a recent study performed in blood of MS patients found an increase of CD5+ memory B cells in remitting stage of the disease . Villar  concluded that increased percentages of blood CD5+ B cells were associated with further elevated risk of conversion to MS and relapse rate in these patients independently of cerebrospinal fluid oligoclonal bands presence and MRI. She calculated a cut off value of 3.5% of this subset for relapse risk. This value was based on mean +/− 2 SD of the percentage of blood CD5+ B cells of the control group. The association of blood CD5+ B cells with the conversion to MS suggests that this lymphocyte subset plays an important role in early phases of the disorder. We planned to use the same method for a cut off calculation, but, as we were looking for an individual predicted outcome, we used a different one.