(A) The protein levels of CBX7 in four classic GBM cell lines were examined through western blot assay (up panel). After transfection with siRNAs or lentivirus vectors, CBX7 expression in three GBM cell lines was assessed by western blot (down panel). (B) Respective merged images of U251, U87 and LN229 cells transfected with siRNAs or lentivirus vectors. Data are means of three independent experiments + SEM. *P < 0.05. Scale bar = 200 mm. (C) Proliferative abilities of GBM cells infected with siRNAs or lentivirus vectors were tested via CCK8 assays. *P < 0.05, #P < 0.001. (D, E) Wound-healing assay and transwell assay were performed to evaluate the migrative and invasive ability of GBM cells transfected with siRNAs or lentivirus vectors. *P < 0.05, #P < 0.001.