Furthermore, ECFP based similarity matrix (Figure 2) clearly shows the overall Tanimoto similarities between the bitterants and non-bitterants are quite low with the average value (0.0694) over the whole matrix. This provide an important clue that ECFP fingerprint may be a good molecular descriptor for our bitterants and non-bitterants classification. Thus in our work, various ECFP fingerprints are implemented and explored.