Two kidneys from WT and CalpTG, aged and young, mice were dissociated by using a gentleMACS Dissociator (Miltenyi Biotec). Tissue was then passed through a 30 µm sieve. Cells were platted during 30 mn at 4 °C with mouse Fc Block (Miltenyi Biotec). Antibodies were incubated during one hour at +4 °C before analyses with flow cytometry MACSquant analyser (Miltenyi Biotec). Antibodies used were: mouse anti-CD45 PERCP (130-102-469, Miltenyi Biotec), mouse anti-F4/80 PE (130-102-422, Miltenyi Biotec), mouse anti-CD11c APC(17-0114, eBioscience), mouse anti-CD45 vioblue (130-102-430, Miltenyi Biotec), mouse nti-CD3 Violetfluor 450 (75-0032, Tonbo biosciences), mouse anti-Prominin1 APC (130-092-335, Miltenyi Biotec). Results are expressed as ratio of immune cells/tubular cells (prominin1 positive cells), an internal control allowing to quantify immune cells infiltrate in the whole kidney.