In this study, we show that miR-101 regulates SUB1 expression and SUB1 has a role in prostate cancer growth. We and others have earlier shown that reduced expression of miR-101 leads to overexpression of oncogenic histone methyltransferase EZH2 in multiple tumors.11, 13, 36, 49 A genomic loss of miR-101 or epigenetic silencing leads to reduction in miR-101 expression in multiple cancers.50, 51 These observations suggest that attenuation of miR-101 expression is an important event in oncogenesis. Our investigations also demonstrate the role of SUB1 in prostate cancer cell proliferation and invasion.