To further address a role of GK in feeding behavior, the time that three group of animals spent eating (after fasting) was measured using a system that allowed individual register ad libitum feeding and daily recording of food intake. During the first meal, unoperated animals spent 22 ± 8 min eating, and Ad-shβGal-EGFP-injected animals spent 35 ± 5 min eating. Ad-shGK-EGFP-injected animals spent 78 ± 22 min eating, which was highly significant compared with both control groups, no differences were observed between control groups, suggesting a recuperation of the feeding behavior (Fig. 6B). The meal frequency in GK knockdown animals (expressed as a number of meal events in 12 h) was approximately double the frequency found in the control groups (Fig. 6C). In addition, the cumulative eating time was also increased in Ad-shGK-EGFP-injected animals (Fig. 6D). Although the number of intervals was not affected (Fig. 6E), the interval duration per animal was decreased in GK knockdown animals, indicating a decreased satiety in this group (Fig. 6F). No differences were detected between non-injected and Ad-shβGal-EGFP-injected animals in all parameters.