For transcriptome work, subterranean clover cv. Daliak plants were sown on 1 May 2015 in the field at Shenton Park, Western Australia (31o57′S, 115o50′E). Five different tissue types (roots, stem and peduncles, leaf and petioles, flowers and developing seeds) were harvested from a single Daliak plant on 24 September, when it was flowering and setting seeds. All samples were taken between 10.00 am and noon to eliminate any diurnal variations.