The crude extract of S. artemisiae MCCB 248 was first subjected to a C18 SPE clean-up step, and then analyzed by positive ionization LC–MS/MS analysis. Four major chemical components were determined to be present in the crude extract. These four molecules had mass spectra indicating protonated mass ions of m/z 761, 827, 879, and 913, and appeared to possess a common UV chromophore (ν = 312 nm, broad absorption from 280 to 340 nm, and ν = 240 nm). These MS/MS spectra did not match with any library entry upon molecular networking. However, they clustered together in a small molecular family, and upon library comparison with the analog search option, were suggested to be structurally related to a polyhydroxy macrolide, such as bastimolide A (Shao et al. 2015). A number of minor metabolites were also detected in the chromatogram by MS or DAD UV, but were not analyzed further due to their low abundance.