In conclusion, we have developed a VR test system, which can essay a variety of different aspects of visual perception. Using this system, it is possible to quantitatively characterize the behavioral responses of mice towards virtually any type of visual cues with minimal human-animal interaction to enhance the consistency of the tests and minimize test variation. Our results provide important details regarding the training requirement of different visual cues. If combined with a head-fixed in vivo imaging recording approach, it could provide a platform for correlated in vivo study of neuronal activity in the brain and visual perception.