The identification of novel drug targets against A. fumigatus, as a human pathogen, was based on alignment of its proteome with the proteome of S. cerevisiae. The proteomes of these two fungi were extracted from NCBI (https://www.ncbi.nlm.nih.gov/genome/browse/). Three different approaches were used to identify and confirm those sequences that are present in A. fumigatus but are absent or significantly diverge from S. cerevisiae. LAST (http://last.cbrc.jp) has been used as a fast sequence alignment tool to identify unique sequences for A. fumigatus. LAST uses variable-length (spaced) seeds realized by a suffix array. The expected value of 10 (-e84) was chosen to obtain higher sensitivity (-m1000000), and the other settings and parameters were used as default. In the next step, unique sequences from A. fumigatus were compared using suffix tree analysis by Mummer (version 3.0) (http://mummer.sourceforge. net). Minimum maximal match was seven to restrict output proteins, but other settings were chosen as default. Finally, the proteins that were unique to fungi were identified by BlastP (NCBI Blast v. 2.2.1). Modeling was carried out by I-TASSER server (Michigan University, USA). Docking of the compounds was performed by HEX 6.3 software when required.