It was reported that monosex culture has been achieved through sex reversal mediated by steroid manipulations and has been practiced in fish aquaculture . Unlike fish, sexual differentiation in crustaceans is uniquely regulated by the male-specific androgenic gland (AG), which has been detected in male crustaceans. It plays a pivotal role in the regulation of male differentiation and in maintaining the male sexual characteristics [4, 7–13]. So far, the insulin-like androgenic gland hormone (IAG) was identified in many commercially important crustacean species [14–18]. IAG silencing in Macrobrachium rosenbergii proved to be useful in obtaining a full and functional sex reversal species , leading to the production of monosex populations. So far, AG cDNA libraries and transcriptome datasets serve as a basis for further studies [20, 21], but the molecular mechanism pertaining to the related regulatory mechanisms of AG in E. sinensis is still unclear.