(A) Transwell invasion through Matrigel of KPC cell lines treated with control or miR-19-targeting antagomirs at the indicated concentrations. (B) Transwell migration activity of the cell lines shown in (A). (C) Quantitative RT-PCR measurement of baseline mature miR-19 levels in KPC cell lines. (D) Transwell invasion through Matrigel of the human PDAC cell lines MIA Paca-2 and PANC-1 treated with control or miR-19-targeting antagomirs at the indicated concentrations. (E) Transwell migration of activity of the cell lines shown in (D). (F) Quantitative RT-PCR measurement of baseline mature miR-19 levels in MIA Paca-2 and PANC-1 cells; miR-19 levels in the KPC cell line 9415#2 are shown for comparison. Invadopodia rosette formation (G) and FITC-gelatin degradation (H) in the KPC cell lines 9910#1 and 9248#1 treated with control or miR-19-targeting antagomirs. n = 3 for each cell line. p values: * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. (A–F) Error bars represent standard deviation from the mean. (G, H) Error bars represent standard error of the mean.