The combined analysis of the glycan motif (glycomics) and the protein (proteomics) forms the field of glycoproteomics. For such analysis, proteins in the sample are first digested into peptides, followed by glycopeptide enrichment using zwitterionic hydrophilic interaction LC (ZIC-HILIC)93 or alternative approaches.91,94 Recently, the combination of higher energy collision dissociation (HCD) and electron transfer dissociation (ETD) have facilitated direct MS analysis of glycopeptides. HCD fragmentation breaks glycosidic bonds, whereas ETD preserves the glycan attachment and fragments the peptide backbone, providing peptide sequence information.89 Direct analysis of intact glycopeptides has rarely been applied in the cardiovascular context. Our study by Yin et al.89 characterised the glycopeptides of secretomes from human endothelial cells. More recently, we have characterized the glycosylation profile of human cardiac ECM proteins.52