Images of the cells were acquired under a DeltaVision Elite High Resolution epifluorescence microscope, using appropriate filters. The fluorophore for the Amplification Orange kit has an excitation wavelength of 554 nm and an emission wavelength of 579 nm and can be detected using the TRITC filter. A DAPI filter, excitation 360 nm and emission 460 nm, was used for the nuclear staining. Images were captured by a photometrics cool snap HQ2 camera utilizing the image capture software softWoRx version 5.5. Slides were analyzed under a 20X/0.85 oil magnification objective.