10.7554/eLife.22114.020Figure 7—figure supplement 1.Transgenic lines expressing different FLM alleles display differential FLM expression and splicing patterns.(A) Image of an agarose gel with the results of a 3’-RACE PCR. The primer used for 3’ amplification is indicated in Figure 7C. The gel portions from the FLMCol-0, INT6+CAA and PolyA_6xΔ amplification products framed with a dotted line were purified, cloned and subjected to DNA sequencing. (B) Schematic representation of the primers used for the qRT-PCR quantification of total FLM levels (exon 1) and the E1I1p intron 1 sequence-containing transcripts as indicated by arrows below the gene model. The black box corresponds to FLM exon 1, the grey box to the proximal portion of intron 1. Numbering is respective to the A of the ATG start codon. (C) Mean and SD of qRT-PCR analyses of intron 1 sequence-containing transcripts from four replicate pools of independent T2 lines. The relative decrease in E1I1p expression values compared to exon 1 transcript is indicated for each line in the graph. Note that the comparison of the expression values between the fragments are approximations since the primer efficiencies may not be identical. Primer sequences are listed in Supplementary file 7.DOI: http://dx.doi.org/10.7554/eLife.22114.020