Nuclear protein extracts were prepared from AMΦ following the kit instructions of Thermo Scientific (NE-PER Nuclear and Cytoplasmic Extraction Reagents, 78833, Thermo Fisher). AMΦ were harvested with trypsin-EDTA and then centrifuge at 500 × g for 5 minutes. After washing cells twice with PBS, pellet by centrifugation at 500 × g for 3 minutes and discarded the supernatant. Adding CER I (protease inhibitors and PMSF) to the cell pellet, vortex the tube vigorously for 15 s, then incubated the tube on ice for 10 minutes. Added CER II to the tube and vortex for 5 s on the highest setting, incubated the tube on ice for 1 minute. The tube was centrifuged for 5 minutes at 16,000 × g, then transferred the supernatant (cytoplasmic extract) to a clean pre-chilled tube. Nuclei pellet was suspended in NER and vortex for 15 s. Placed the sample on ice and continued vortexing for 15 s every 10 minutes, for a total of 40 minutes. After centrifuged at 16,000 × g for 10 minutes, supernatants containing nuclear proteins were frozen in liquid nitrogen in small aliquots and store at −80 °C. Protein quantification was performed using BCA protein assay.