To select the best ISSR primers for A. flaccida in Step 1, each amplified locus was evaluated according to the presence (1) or absence (0) of the DNA fragments (band). Based on the binary matrix obtained, the discriminatory power of ISSR primers was evaluated using three parameters: (i) polymorphism information content (PIC), (ii) marker index (MI), and (iii) resolving power (RP).