In most moths, the biosynthesis and release of sex pheromone are precisely regulated by PBAN. The PBAN signal transduction in B. mori has been elucidated in impressive detail for complete genomics information of B. mori. A series of genes responsible for sex pheromone biosynthesis and release have been well documented. Nonetheless, as a model species, the detail molecular mechanisms of B. mori sex pheromone biosynthesis are not fully elucidated. In this study, iTRAQ-based proteomics was employed to study the developmental expression profile of PGs, the targeted tissues of PBAN. The results revealed a series of differentially expressed proteins during PG development. These differentially expressed proteins, including fatty acid transport protein, acyl CoA desaturase, acyl-CoA binding protein, fatty-acyl reductase, perilipin, Orai1 alternative splice form A, calcineurin A and acyl carrier protein, were involved in the developmental regulation of sex pheromone biosynthesis and release. They served as marker proteins for the biosynthesis and release of sex pheromones, as being confirmed in B. mori. The proteomics results were consistent with the previous results, in which the transcripts of these proteins were richly expressed at key stages of sex pheromone biosynthesis57810151617.