The hypothalamus is involved in the control of feeding behavior due to its ability to detect and respond to changes in blood glucose levels30. Particularly the AN, which is located in the basal region of the hypothalamus, is proximal to the ME, a circumventricular organ known to play an important role in homeostatic regulation31. Interestingly, tanycytes link the ventricular and the vascular compartments of the ME and regulate the permeability properties of the fenestrated capillaries of the ME31. Unlike the fenestrated portal vessels of the ME, which are permeable to molecules coming from the bloodstream, adjacent tanycytes present various types of tight junctions, which prevent diffusion of substances to the rest of the brain through the CSF. In this manner, blood-borne molecules enter the parenchyma of ME through the permeable vessels, and β2-tanycytes control their diffusion to the CSF via their tight junctions31. A different subpopulation of tanycytes, β1-tanycytes, is in close contact with hypothalamic neurons, particularly with the glucose-responsive populations of the AN. This particular distribution of hypothalamic tanycytes represents a key point in the regulation of food intake, and we have previously demonstrated that tanycytes express GLUT2 in their proximal pole (in contact with CSF) as well as GK and ATP-sensitive potassium (KATP) channels12.