Cell lines - Mosquito C6/36 HT cells obtained from the ATCC were cultured as previously described (Castillo et al. 2016). Vero cells (CCL-81) were grown in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% heat-inactivated foetal bovine serum (FBS), 1% L-glutamine, 1% vitamins, 1% non-essential amino acids, and 1% Penicillin/Streptomycin (Sigma-Aldrich Chemical Co, St. Louis, MO, USA), at 37ºC with 5% CO2.