Image J software (NIH) was used to quantify the band intensity. For RT-PCR of SMN2, the intensity of full-length bands and ∆7 bands were measured. The rate of exon 7 inclusion was calculated by the intensity value of (full-length)/(full-length + ∆7 SMN2). The RT-PCR, the qPCR, and the Western blotting data were analyzed by one-way repeated measures ANOVA with Tukey’s or Dunnett’s multiple comparison post hoc analysis by GraphPad Prism 7 (GraphPad software).