Paracrine factors showed alterations in their mRNA expression levels when the SCF gene expression was inhibited. Similar experiments under identical conditions were conducted again with the addition of UVB exposure. Exposure to UVB is known to induce expression of SCF, so its effect on mRNA levels compared to the levels without UVB exposure would be a necessary observation. Comparisons between the paracrine factors with and without SCF knockdown, and with or without UVB, emphasize the individual gene expression variations not only in terms of SCF differences but also in determining whether UVB causes certain factors to react and express differently. In Figure 2, the results show the upregulation gene expressions of SCF, HGF, NRG, and CRH with UVB exposure to regular fibroblasts compared with the control group, which did not undergo UVB stimulation. The ET-1 gene was downregulated, and no significant difference was observed with DKK-1. Following the determination of the influence of UVB on regular fibroblasts, the different outcome in fibroblasts with SCF gene knockdown under UVB stimulation was tested. A comparison between regular fibroblasts with UVB treatment and SCF gene knockdown fibroblasts with the same UVB treatment is presented. The data presented the upregulation mRNA expression of HGF, NRG, and CRH and their expression levels when the fibroblast cells, specifically with stable SCF silencing, were exposed to UVB. Compared to the regular fibroblast gene expressions with UVB exposure, HGF, CRH, and NRG with SCF knockdowns all had their mRNA expressions increased, even with the same UVB treated situation. HGF demonstrated a particularly dramatic increase in gene expression levels in cells with SCF genetic knockdown, while DKK-1 and ET-1 were the SCF-silenced genes that were downregulated when treated with UVB. SCF gene alteration influenced the fibroblast paracrine system and the condition differed with or without UVB stimulation.