From the screen results for the arl1Δ strain, we selected COG4, TAX4, SNX4, and SLT2 as candidates for further testing based on their cellular functions as important regulators of membrane traffic or autophagy (see Table S2). We obtained ARL1 from the screen, a good internal control. Also, YPT6 was identified from the screen, which was previously confirmed as a suppressor of the arl1Δ strain (Yang and Rosenwald 2016). For genes of interest contained on library plasmids with several genes, we purchased the relevant single genes from the ORF collection (Gelperin et al. 2005). For the COG4 and SNX4 plasmids obtained from the screen, each of these plasmids contained only the gene of interest, thus we used these two plasmids directly in additional assays. For the screen results of the ypt6Δ strain, we selected IVY1, ATG5, and PEP3 (see Table S3). We also obtained a fragment with YPT6, again a good internal control. In summary, we selected a total of seven genes to be tested specifically for suppression of the autophagy defects. All seven plasmids containing single gene candidates were transformed into the arl1Δ and ypt6Δ strains.