Meiotic chromosomes were prepared from the testes of two males. They formed 21 bivalents and one univalent (X) (Figure 6c,d). C-banding technique revealed in autosome bivalents C-bands as described above. In the X chromosome, small C-bands were visible at telomeric position in both arms while in the pericentric region no C-positive region was detectable (Figure 6c). FISH with the labeled fragment of 18S rDNA and telomeric repeats intensively painted one large extended region on pachytene chromosomes. This corresponded to the short arm of a large submetacentric chromosome. Other regions were painted with labeled 18S rDNA only slightly. On pachytene chromosomes, no ITSs were found (Figure 6d). In Figure 5e, a karyotype is presented; gonosomes were identified here.