Having shown that the combinatorial vaccination improves the humoral IgG2a antitumor response and the long-lasting protection against melanoma as compared to trAb immunization alone, we analyzed in more detail the T-cell compartment in differentially immunized mice. To discriminate effector, effector-memory and central-memory cells, T cells isolated from immunized mice and restimulated in vitro were analyzed for expression of CD44 and CD62L (Figure 5A). It turned out that the fraction of memory CD4+ T cells was markedly increased in the group having undergone the combinatorial vaccination (Figure 5B).