Differential expression of the CsMRP4 and reactivity of mouse anti-NBD1 immune serum. a Relative mRNA level of CsMRP4 gene in the adults and metacercariae of C. sinensis, measured using Q-rt.-PCR. b Reaction detected by western blot and immuno-ECL. The mouse immune serum reacted well with rCsMRP4-NBD1 (left) and specifically detected native CsMRP4 (right) in the crude extracts of C. sinensis. Lane NBD1: mouse anti-NBD1 immune serum; Lane Normal: normal mouse serum; Abbreviations: R, recombinant protein; N, native CsMRP4