This study has so far optimized the decellularization process for human auricular cartilage. This technique can be incorporated further with biofabrication strategies in developing hybrid scaffolds for auricular reconstruction. Biofabrication through the use of 3D printing has been able to recreate the complex shape of human ears28 and enabled targeted cell seeding as well as growth factor delivery29. It has allowed for the use of hydrogels also termed “bio-inks” to be coated on to three dimensional ear scaffolds providing an aqueous environment in which chondrogenic differentiation of stem cells can occur12. Bio-inks can also be constituted by soluble forms of decellularized extra cellular matrices which have shown to be more biocompatible options30. By optimizing the production of a decellularized ECM, this can be combined with bioprinting strategies to improve the overall process of auricular regeneration.