The possibility to combine PTM and SCS, either in E. coli or in L. lactis, is good news for the development of new antimicrobials, since it allows researchers to benefit from several advantages. For both organisms, high- and low-copy vectors with inducible and constitutive promoters are available, allowing fast construction of the aimed construct and a high combinatorial potential (Baumann et al., 2017). A short generation time allows fast experiments and fast results. In special cases, sophisticated expression setups even allow production to outcompete the natural host (Kunji et al., 2003; Sezonov et al., 2007; Ongey and Neubauer, 2016).