The activity of Wa1F1-KT was tested against the reference C. lusitaniae NEQAS 6208 strain and fluconazole-susceptible or -resistant clinical isolates as well as laboratory strains of C. albicans and C. glabrata. Each test strain, grown overnight on SDA plates, was resuspended in water to a final concentration of 0.5 McFarland and spread (100 µL) on the surface of YPD agar plates. Crude extracts from W. anomalus 1F1 were poured into wells of 8 mm (40 µL per well) cut into the agar plates. The plates were incubated for 48 h at 25, 30, or 37 °C and the diameter of the area of growth inhibition was measured.