Initially, we evaluated separately the frequency of IFNγ, TNF, IL2 producer CD4+ and CD8+ T lymphocytes, regardless of their simultaneous production (Fig. 2b–d, g–i). First, we observed a trend toward an increased frequency of total IFNγ+CD4+ T cells in DENV (exception of patient 2, open squares) compared to exposed donors. DENV/ZIKV‐patients and exposed healthy donors had similar frequencies of total IFNγ+CD4+ T cells (Fig. 2b). Exposed donors, DENV‐ and ZIKV‐patients had a similar frequency of total TNF+CD4+ T cells, while DENV/ZIKV‐patients had significantly decreased frequencies of total TNF+CD4+ T cells compared to exposed donors and DENV‐patients (Fig. 2c). A trend toward decreased frequencies of total IL2+CD4+ T cells was seen in all acute patients compared to exposed donors (Fig. 2d).