IPA analysis results revealed that apoptosis induction for MCF-7 cells is an important anti-cancer molecular mechanism of Cos, Dehy, CD, and VOSL. In this study, we further validated the IPA analysis results based on Annexin V-FITC/PI apoptosis analysis. The results of cell apoptosis analysis were shown in Fig. 6C and D. Cos, Dehy, CD and VOSL treatment all can dose-dependently induce MCF-7 cell and MDA-MB-231 cell apoptosis, moreover, the effects of apoptosis induction in the CD or VOSL-treated group are stronger than those in the Cos or Dehy-treated group.