We attempted to predict the 3D model of CsTegu20.6 based on homology modeling using Swiss-Model . However, the first attempt failed because the proper template structures were not found in the protein data bank (PDB) template library, as experimental structures of helminth tegumental proteins were not available. The search for templates showed a very low percentage of sequence identities (<25%) and coverages (<51%). We next attempted to predict a CsTegu20.6 model based on multiple-threading alignments using I-TASSER . The 3D model was constructed using 10 multiple templates and refined with energy minimization. However, the second attempt also was not successful because the Ramachandran plot for the model showed 64.8% residues in the most favored regions and 3.8% residues in the disallowed regions (Figure S2).