The targeted regions of genes related to pancreatic cancer are shown in S1 Table. The sequences of the adapters and primers are shown in S2 Table. A 30-base-long adapter sequence, including the primer sequence for Ion Torrent sequencing, was joined to a 5-base sequence for indexing individuals, a 12-base sequence for indexing molecules, and a 20-base spacer at the 3ʹ end.