We appreciate this comment, and in fact did the excess biotin trap experiment before submission for the lagging strand streptavidin blocks. On hindsight we should have included that data in the original submission. In the revised manuscript, we perform both leading and lagging experiments using a biotin trap (and 32P-label on the biotinylated strand), and using the forked DNAs with equal 6bp spacing between the two biotin-streptavidins for both leading and lagging strands. The results show that Mcm10 enables bypass of the lagging strand blocks without displacing the streptavidin blocks, and that the leading strand streptavidins are in fact displaced from DNA (there was less efficient leading strand read through with the 6 bp spacing compared to the earlier 14 bp spacing). These data are now presented in a newly added Figure 6 and supplement to Figure 6, which shows the effectiveness of the biotin trap. The modified manuscript reads as follows: