Duolink™ PLA experiments rely on the selection of two primary antibodies (preferably immunohistochemistry, immunocytochemistry or immunofluorescence validated) that must be raised in two different species (for instance mouse and rabbit). Primary antibodies should be from IgG-class and specific for the target to be detected. PLA can use either monoclonal or polyclonal antibodies. We have tested several antibodies (Table 1).Table 1Antibodies used for PLA assayAntigenSpeciesNameConcentrationReferenceRUNX1mouseAnti-RUNX11 mg/mLab110035 (Abcam)RUNX1rabbitAnti-RUNX11 mg/mLab23980 (Abcam)ETV6mouseAnti-ETV60.5 mg/mLab54705 (Abcam)ETV6rabbitAnti-ETV60.2 mg/mLsc11382 (Santa Cruz biotechnology)ETV6rabbitAnti-ETV60.2 mg/mLsc166865 (Santa Cruz biotechnology)CBFBrabbitAnti-CBFB1 mg/mLab133600 (Abcam)