AHG was prepared as the protocols previously described . RMPI 1640 was purchased from Hyclone (Thermo Fisher, Shanghai, China). Fetal bovine serum was purchased from Gibco (Thermo Fisher, Shanghai, China). Con A, LPS, penicillin and streptomycin were purchased from Sigma-Aldrich (St. Louis, MO, USA). CY and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Solabio (Beijing, China). Anti-mouse FITC-labeled CD 80 monoclonal antibodies was purchased from eBioscience (San Diego, CA, USA). Mouse IL-2, IL-4, TNF-α, IFN-γ Enzyme-Linked Immunosorbent Assay (ELISA) kit was obtained from Dakewe (Beijing, China). Fluo-3/AM fluorescent probe and BCA protein assay kit was purchased from Beyotime (Shanghai, China). Dimethyl sulfoxide (DMSO) was purchased from Sangong Biotech (Shanghai, China). GSH-PX, SOD, CAT, T-AOC and MDA kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Injectable LNT, used as positive control, was purchased from Nanjing Easeheal Pharmaceutical Co., Ltd. (Nanjing, China).