To better evaluate levels and localization of Cx43 in SH‐SY5Y cells we performed Western blots and immunofluorescence analysis at different time‐points. These experiments confirmed high levels of this marker from the end of the injury (3 hrs) in untreated SH‐SY5Y cells (Figs. 3B and 4A, B). The expression levels appeared to increase markedly after 8 hrs and then clearly decrease at 24 hrs (Fig. 3B) in both intracellular and extracellular compartments (Fig. 4B). However, our data showed that H/R cultures, in absence of OEC‐CM, had higher levels of Cx43 at each analysed time‐point compared to the cultures in normoxia with and without OEC‐CM. On the contrary, in injured cultures, in the presence of OEC‐CM the expression levels of Cx43 seemed unaffected along the different time‐points (Figs. 3A and B, 4B) compared to the cultures in normoxia with and without OEC‐CM. Moreover, H/R cultures exposed to OEC‐CM exhibited an evident reduction of Cx43 expression when compared to the unconditioned injured cultures (Figs. 3B and 4B).