10.7554/eLife.22114.015Figure 4—figure supplement 1.Increasing the number of PolyA motif deletions results in the gradual reduction of FLM-ß expression.(A) Schematic representation of the FLMCol-0 reference construct (pFLM::gFLM) as well as the FLM PolyA deletion variants selected for transgenic analysis in Nd-1. Deletions of the PolyA motifs are displayed with red lines (not drawn to scale). (B) and (C) Mean and SD (four replicate pools comprising between five and ten independent T2 transgenic lines) from qRT-PCR analyses of FLM-β (B) and FLM-δ (C) expression in ten day-old seedlings. Student’s t-test: *p≤0.05; **p≤0.01; ***p≤0.001; otherwise not significant.DOI: http://dx.doi.org/10.7554/eLife.22114.015