To examine total chlorophyll content in Chrysanthemum leaves, 500 mg of leaf samples was harvested and extracted with 5 ml of aqueous acetone (80%, v/v), and then centrifuged at 12,000 rpm for 15 min. Absorbance of the supernatant was recorded at wavelengths of 645 and 663 nm, respectively. The amounts of chlorophyll in leaves were calculated according to the formulae: 8.02 × A663 + 20.21 × A645 as reported previously by Porra (2002).