By adopting this pooling strategy, we have followed procedures that were described in publications from leading flowering time labs (e.g. Coustham, 2012, Science; Caroline Dean lab). This is an attractive way to reduce the variability of transgene expression that result from positional effects in different transgenic lines. In view of the ample analysis of transgenic lines presented in our manuscript, this presented the most efficient way for reducing cost and time while still producing a meaningful data set. If we had used T3 generation plants, we would have had to propagate and test the segregation of over 3000 individual plants. We have modified (and thereby improved) the above-described strategy by pooling plants into four pools, which we designate replicate pools. We have also verified the trustworthiness of this pooling system by demonstrating that it is possible to precisely recapitulate the effects observed with the Col-0 and Kil-0 FLM alleles (Figure 2—figure supplement 2C and D). The statistical analysis has now been integrated into the figure as requested by the reviewer.