Since there was no significant difference between the results from the first and second sample sets, subsequent analyses were performed by combining data from both sample sets. To examine the contribution of individual genes (after application of the CV78 filter), we plotted the cumulative number of variant-positive patients identified for each gene (Fig 2). In Fig 2A, the genes are arranged along the x-axis in descending order of the number of variant-positive patients identified. Conversely, in Fig 2B, the genes are arranged along the x-axis in descending order of the number of variant-positive patients identified per gene. Fig 2A shows that the highest number of variant-positive patients had variants in KRAS (explained below). In addition, only a small number of variant-positive patients was removed after applying the CV78 filter. Fig 2B shows that the numbers of selected and removed variant-positive patients increased as the number of genes increased. Using variants in all genes except KRAS identified the same number of filtered variant-positive patients as variants in KRAS only, and the numbers of variant-positive patients after filtering and their proportions among the total number of patients (143) were as follows: all genes, 51 (35.7%); KRAS, 37 (25.9%); other genes excluding KRAS, 37 (25.9%).