Recombinant (r)CsTegu20.6 was purified under native conditions using a histidine tag at the N-terminus. The observed molecular weight was consistent with the predicted molecular mass (Figure 8A). The purified protein was verified using a monoclonal anti-His antibody (Figure 8B). The liquid chromatography-mass spectrometry performed according to our previously reported method  identified six specific peptide fragment sequences: 31NNIDPSMIKRWQVLFDADDSGVITLDEFCK60, 41WQVLFDADDSGVITLDEFCK60, 82GPSLPREVDVITATLPLDQQVDIVNEVMR110, 88EVDVITATLPLDQQVDIVNEVMR110, 114NEPFDENLVSK124 and 145GSSWCSFSYEPK156. The peptide sequences matched with those predicted for CsTegu20.6 with a sequence coverage of 56% (Table S2).