Sbp1, Pab1, and eIF4G1 interact with each other and formation of the ternary complex is RNA-dependent. IgG-pulldown assay showing that Sbp1, Pab1, and eIF4G1 interact in the presence of 5′UTRPab1 (cf. lanes 2 and 3), and the molecular interaction is disrupted by RNase treatment (cf. lanes 3 and 4). Lane 1 shows that Sbp1 and eIF4G1 do not interact with each other when Pab1 is absent.