In this study, 341(90.5%) patients in IHC and 89 (95.7%) in qPCR were followed up. But we only included 325 (86.2%) patients in IHC (Sox2: 86.0%, 264/307) and 80 (86.0%) patients in qPCR with R0 resection to perform the survival analyses. The median survival time (MST) and 2-year overall survival rates of different groups of these markers in IHC and qPCR were shown in Table 5. The MST was not applicable when the survival rates at the end of follow-up time were still higher than 50%.