Acquired immunodeficiency syndrome (AIDS) is one of the world’s most serious public health concerns, and poses an enormous challenge to most countries, especially developing countries such as Nigeria. Nigeria carries the second heaviest burden of HIV in Africa and has an expanding population of people living with HIV/AIDS (PLWHA) (Federal Republic of Nigeria, 2012). The first HIV Sentinel Survey in Nigeria in 1991 showed a prevalence of 1.8%. Subsequent sentinel surveys produced prevalence of 3.8% (1993), 4.5% (1996), 5.4% (1999), 5.8% (2001), 5.0% (2003), 4.4% (2005), 4.6% (2008) and 4.1% (2010) (Federal Republic of Nigeria, 2012). Health care workers (HCWs) are key players in the management of HIV infection because it is through the health infrastructure that individuals can learn of their HIV status, obtain prevention education, and seek treatment and care (Kiragu et al., 2007). The attitude of HCWs influences the willingness and ability of PLWHA to access care and influences the quality of care they receive (Kermode, Holmes, Langkham, Thomas, & Gifford, 2005). Discrimination against HIV-positive patients has profound impact on the care and support required for their optimal management particularly in resource-constrained settings (Sadoh, Fawole, Sadoh, Oladimeji, & Sotiloye, 2006). Stigmatization and discrimination impact negatively on interventions and act as barriers to adherence to antiretroviral therapy among PLWHA (Omosanya, Elegbede, Agboola, Isinkaye, & Omopariola, 2013). PLWHAs in Nigeria have been found to be subject to discrimination and stigmatization in the workplace, by family and communities (Reis et al., 2005) and may also face discrimination from HCWs (Famoroti, Fernandes, & Chima, 2013; Omosanya et al., 2013). Discriminatory or unethical behavior may hinder effective prevention and treatment by discouraging individuals from being tested or seeking information on how to protect themselves and others from HIV/AIDS.