As part of serial studies to identify the role of the key bacteria in several associated diseases, this study aims to:Develop anti-bacterial human antibodies in vitro from patients who have been treated for systemic infection.Immobilize the produced antibodies in a SPR system and use the response to injected heat-killed pathogens for assessment of specificity.Analyze the ulcer secretions from patients and healthy volunteers by the SPR system and compare the results to data from culture methods.To use these antibodies as an alternative tool for detecting bacteria in biofilms.