The homogenate of liver, heart or kidney was prepared in 0.1 g/mL wet weight of ice-cold physiological saline. The samples were centrifuged at 2000 g at 4 °C for 10 min, and the supernatants were collected for the measurement of protein, T-AOC, MAD, CAT, SOD and GSH-Px. These antioxidant parameters were determined by commercially available assay kits in accordance with the manufacturer protocols.