In the present study, we have compared the metabolic profiles of PDAC and CP in blood in two independent cohorts using untargeted LC-MS. The fact that both cohorts did not consist of either serum or plasma is a drawback of the study. However, that they represent completely independent patients strengthens the validity of the proposed biomarkers. Previous reports comparing the metabolite profiles of serum and plasma samples have found good correlation between the two sample matrices (Denery et al. 2011; Yu et al. 2011; Ishikawa et al. 2014). We have identified three single discriminative metabolites as well as a five-metabolite panel of markers, since a marker panel can increase specificity and sensitivity compared to single discriminative metabolites (Wang et al. 2011; Wingren et al. 2012). All five metabolites identified here can be easily measured in a clinical MS-lab.