TASK-2; Our qPCR analysis indicates only minimal expression of any TALK type channels in the adult mouse retina. Consistent with our mRNA studies, we could not detect expression of TASK-2 protein in the adult mouse retina with the antibody used in this study (Fig. 6D). However, strong TASK-2 immunoreactivity was observed in the conjunctival epithelium which covers the outer surface of the sclera (not included in retina samples used for mRNA analysis) (Fig. 6E,F). Collectively, our data indicate a lack of TASK-2 expression in the adult mouse retina. TALK-1 and THIK type channels; Due to lack of detectable mRNA expression in the retina we have not investigated the expression of TALK-1 protein, nor the expression of THIK type channel proteins in the mouse retina.