In 275 patients with positive N stage, we collected 206 (74.9%) metastatic LNs to perform IHC. There were 24 (11.7%) patients in CD54, 45 (21.8%) in Lgr5, 23 (11.2%) in CD133, 43 (20.9%) in Oct4 and 167 (81.1%) in EpCAM in high expression groups. For Sox2, out of 223 patients with positive N stage, 185 (83.0%) metastatic LNs were collected to be investigated by IHC, and the high expression rate was 33.5% (n=62). All the expressions of these markers in metastatic LNs had significantly relation to the expressions in primary lesions (all p<0.001).