Calpastatin overexpression protects against cardiovascular aging. Mean media surface of interlobar arteries was significantly less increased in CalpTG (TG) mice at 2 years in comparison to WT mice (A,B,E, **p < 0.01, n = 10/group, magnification ×400). Similarly, aorta remodelling was less important in CalpTG mice (C,D,F, **p < 0.01, n = 10/group, magnification ×200). Heart weight increased with aging but less in CalpTG mice than in WT animals (G, *p < 0.05, n = 10/group). This increase in heart weight was due to cellular hypertrophy since the number of nuclei per field at 400x magnification was significantly lower in WT in comparison to CalpTG mice (H, ***p < 0.001, n = 5/group).