The aim of this study was to produce (−)-α-bisabolol by large-scale fermentation of E. coli, which has been widely used for various industrial applications, and to develop an economically viable direct (−)-α-bisabolol extraction process during fermentation. To achieve this, we created (−)-α-bisabolol-producing E. coli in three steps: (1) We introduced the German chamomile (−)-α-bisabolol synthase gene (MrBBS) into E. coli convert endogenous FPP to (−)-α-bisabolol; (2) We engineered the exogenous MVA pathway to increase IPP and DMAPP pool; and (3) We overexpressed an ispA gene encoding an FPP synthase that efficiently provides the (−)-α-bisabolol precursor (FPP) from IPP and DMAPP (Fig. 1b).