Normalising DNA content to the percentage reduction in the wet weight fraction there was a consistent decrease at the end points of the three protocols (Fig. 7) correlating with a decrease in DNA content. Protocol A at day 35 underwent the highest reduction (0.79 fold) in DNA content compared to native and equally showed the greatest percentage reduction in wet weight fraction. The same trend was observed in in the case of protocol B and C at 14 days with the former showing a greater decrease in DNA content and reduction of the wet weight fraction.