Together, our findings demonstrate important roles for mir-17~92-encoded miRNAs during early stages of pancreatic tumorigenesis, as well as tumor progression and invasion. These findings provide functional support for the observed elevated expression of these miRNAs in precursor PanIN lesions and invasive PDAC. Further dissection of the cluster to identify the roles played by individual miRNAs during PanIN maintenance and PDAC invasion will be required in order to identify the critical target genes and pathways regulated by this miRNA cluster during pancreatic tumorigenesis.