To assess if the HAdV5-PUMA and BVCARHAdV5-PUMA vectors remain localized within or escaped from the injected joints, we performed PCR assays on total DNA extracted from liver samples and sera from rats killed at day 4 and day 21 after treatment. No amplification of either adenovirus or baculovirus genome were obtained from the liver and serum samples. These results implied that there was no detectable dissemination of either vectors from the injected joints.