Our experiments performed by immunofluorescent microscopy were reproduced by the Skoufias’ lab and presented in the DeBonis’ paper1 with references to our reported data. The single experiment that was not reproduced is the in vitro dimerization of the human recombinant TPPP/p25 in the absence of tubulin/MTs1. By studying carefully the differences of the experimental conditions used in the two labs we recognized a critical point causing the discrepancy, namely that all experiments performed in relation to the dimerization of TPPP/p25 by Skoufias and co-workers were carried out at reducing conditions (in the presence of DTT) that counteracted the stabilization of the dimers by disulphide bridges4. The spontaneous formation of the intermolecular covalent bridges at room temperature above 10 μM TPPP/p25 concentration can ensure the detection of dimers even by SDS/PAGE4. However, it is important to emphasize that TPPP/p25 dimers were detected in our lab, but not in Skoufias’s lab, by sandwich ELISA, isothermal titration calorimetry and size exclusion gel chromatography as well48.