Total RNA was extracted from AMΦ and lung tissues using the TRIzol reagent (Sigma-Aldrich) and following the manufacturer’s instructions. Total RNA was then reverse transcribed using a PrimeScript RT reagent kit (TaKaRa Bio Inc. Shiga, Japan). Primers for TLR3 amplification (162 bp) were: position 91 forward 5′-GTGAGATACAACGTAGCTGACTG-3′, position 231 reverse 5′-TCCTGCATCCAAGATAGCAAGT-3′. Primers for MIP-2 amplification (108 bp) were: position 62 forward 5′-CCAACCACCAGGCTACAGG-3′, position 169 reverse 5′-GCGTCACACTCAAGCTCTG-3′. Primers for β-actin amplification (154 bp) were: position 163 forward 5′-GGCTGTATTCCCCTCCATCG-3′, position 295 reverse 5′-CCAGTTGGTAACAATGCCATGT-3′. The product of reverse transcription was amplified by following the Premix Taq Version 2.0 instructions (TaKaRa Bio Inc.). PCR products were separated using a 2% agarose gel and identified by SYBR green staining. Expression of mRNA was quantitated using Image Lab software (Bio-Rad) and normalized to the β-actin signal.