Plants of Cucurbita pepo subsp. pepo cultivar-group Zucchini, variety True French, were grown in greenhouse facility at Department of Agricultural Science of University of Naples “Federico II” using standard horticultural practices. C. pepo cv. True French tissue samples were collected from young plants of about 10 cm high. Total RNA was isolated from ground, frozen leaf tissues using the SpectrumTM Plant Total RNA Kit (Sigma-Aldirch). A complete removal of traces of DNA was performed using On-Column DNase I Digest Set (Sigma-Aldirch). Quantity and integrity of the extracted total RNA were determined using NanoDrop ND-1000 Spectrophotometer (Thermo Fisher Scientific Inc., USA), on a denaturing formaldehyde gel and Agilent 2100 bioanalyzer (Agilent Technologies, USA) respectively, to be RIN > 8. Library preparation and sequencing were performed by the Genomix4Life S.r.l., spin-off of Salerno University. The sequencing library was prepared using the TruSeq RNA Sample Preparation Kit v2 (Illumina, San Diego, CA, USA) and paired-end reads of 100 bp were sequenced from the three independent samples on one lane of an illumine HiSeq 2000.