Li, et al.21 (2015) compared CH removal efficacy of conventional needle irrigation, sonic, ultrasonic activated irrigation, and Photon-Induced Photoacoustic Streaming methods in root canals and isthmuses. Study results showed that laser and ultrasonic activated irrigation protocols were the most effective methods on the elimination of CH in both apical region and isthmuses. The findings of Li, et al.21 (2015) support the results of our research. According to our results, PUI was the most effective method for removal of CH than needle irrigation techniques in all three root regions. In addition, no statistically significant difference was found between XP-endo Finisher and PUI groups in the coronal and middle regions of root canals. However, Leoni, et al.20 (2016) stated that no significant differences were found between PUI and XP-endo Finisher in removing debris from root canal surface.