The prepared constructs were used to transform A. fumigatus KU80ΔpyrG strain as described before. Positive transformants were selected on Vogel’s minimal medium lacking uracil/uridine supplements. To find the knock out strains, transformants were screened by PCR using primers RBP-F and RBP-R (Table 1). As the silencing construct, pRBP_RNAi did not contain any fungal selection marker, the pRG3- AMA1 vector containing the pyrG selection marker was used as the second plasmid in co-transformation reaction. RNAi transformants were also selected based on PCR using gfp- and AfuRbp-specific primers.