Mice were sacrificed, tissue dissected, and fixed in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4. Tissues were then paraffin‐embedded, and sections were used for immunohistochemical staining 4‐HNE (1:200; Abcam) using an immunohistochemistry Select HRP/DAB kit (EMD Millipore). The images were acquired using an All‐in‐One Fluorescence Microscope BZ‐X700 (Keyence). Skeletal muscle was examined for pathology in transverse and longitudinal planes by H&E staining.