Application of Spectral Methods to Assess Gametes, Embryos, and Human Reproductive Capabilities
Relevance of the research topic: At present, in the world, a kind of "plateau" in the efficiency of assisted reproductive technologies has been achieved, which ensures a birth rate of 30% per embryo transfer. At the same time, a relatively high (15-20%) and stable rate of miscarriages is preserved. Until now, no effective methods for assessing the potential of gametes and embryos, as well as human reproductive capabilities, have been offered. In these conditions, to increase the rate of births after IVF, clinicians have to increase the number of transferred embryos at a time, however, this leads to a sharp increase in complications of IVF, such as multiple pregnancy. In addition, until today, the clinical effectiveness of assessing the potential of endometrium using gene expression determination methods has not been shown. Therefore, to ensure the effectiveness and safety of infertility treatment, it is necessary to develop methods for predicting the potential of gametes and embryos, as well as human reproductive capabilities. For this purpose, the investigators assume to use Raman spectroscopy of the environment obtained from the objects of research, as well as fluorescent spectroscopy of endometrium. The objects of the research are gametes (spermatozoa) and embryos, used culture medium, endometrium. The subject of the study is the set of factors, that exists in the objects of research and their ability to determine the outcomes of infertility treatment.
- EligibilityCriteria: Inclusion Criteria:  Embryos: embryos that have reached the blastocyst stage Sperm: samples used for IVF during infertility treatment Endometrium: endometrial spectra in cases where an embryo transfer was performed into the uterus  Exclusion Criteria:  For all groups: ectopic pregnancy Embryos: presence of only one blastocyst, and that embryo prognosed by the model as negative Sperm: a cycle where less than 3 oocytes suitable for fertilization were obtained; total pathological fertilization; 60% or more immature oocytes at the time of fertilization registration - HealthyVolunteers: No - Gender: All - MinimumAge: 20 Years - MaximumAge: 44 Years - StdAgeList: Adult