Renin Angiotensin Aldosterone System In Septic Kids
Prospective observational cohort study; pediatric sepsis vs. healthy pediatric subjects and pediatric sepsis with acute kidney injury (AKI) vs without AKI.

Blood samples and renal ultrasound will be collected on sequential days for septic subject and one time for the healthy patients.

Enzyme-linked immunosorbent assays (ELISA) with be run on serum plasma to compare the renin-angiotensin-aldosterone system (RAAS) between groups.
- EligibilityCriteria: Inclusion Criteria:  pediatric patients age 1 days - 18 years old with sepsis or age 1 day - 18 years and healthy.  Exclusion Criteria:  -pre-existing end stage renal disease (ESRD), chronic renal failure (CRF), home use of angiotensin converting enzyme inhibitor(ACE) or angiotensin receptor blocker(ARB) medications, pre-existing congestive heart failure (CHF), and unrepaired congenital heart disease - HealthyVolunteers: Accepts Healthy Volunteers - Gender: All - MinimumAge: 1 Day - MaximumAge: 18 Years - StdAgeList: Child, Adult - StudyPopulation: Pediatric subjects, age 1 day to 18 years. Septic cohort will meet criteria for sepsis (pSOFA >/= 2 or Phoenix criteria) and healthy cohort. - SamplingMethod: Non-Probability Sample