In 1589, she met her niece Christina of Lorraine in Lyon and escorted her to her marriage with the Grand Duke of Tuscany in Marseilles. In 1597 she married secondly with a French noble, Marc de Rye de la Palud, Marquis de Varambon, Comte de la Roche et de Villersexel, who died one year later, in December 1598. In 1608, she returned to Lorraine to nurse her brother on his deathbed. She remained in Lorraine the rest of her life, dying aged 76 in 1621. She was buried in the Église des Cordeliers, Nancy.