Only one small stream near Nescopeck Creek's mouth had Class-C fishery waters. The central part of Nescopeck Creek, as well as most of Black Creek had Class-D fishery waters. There were twenty species of fish in Nescopeck Creek in 1999. Of these, seventeen had been seen in the watershed before. However, since between a 1999 study of the watershed and the study before that, the brown bullhead and the bluegill fish had vanished from the Nescopeck Creek watershed. A large number of genera of macroinvertebrates have been discovered in and around Nescopeck Creek. These consist of one genus of segmented worm, one genus of sowbugs, 11 genera of mayflies, 8 genera of stone flies, 11 genera of caddisflies, 6 genera of dragonflies, 2 genera of helgrammites, 10 genera of beetles, and one genus of fly. There are a total of 14 species of amphibians in the Nescopeck Creek watershed, of which 11 breed in the watershed. These species consist of 6 salamanders, 6 frogs, one newt, and one toad. There are seven species of reptiles in the watershed, of which five breed there. Five of these species are snakes and two are turtles. The biodiversity of birds in the watershed is much greater than that of amphibians or reptiles; there are approximately one hundred different species of birds in the Nescopeck Creek watershed. A total of 29 mammals have been observed in the creek's watershed, including three species of bats, two species of mice, and two species of foxes. The most common habitat in the Nescopeck Creek watershed is the dry-oak mixed forest. Common trees in this habitat include northern red oak, white oak, and chestnut oak. This habitat also contains gray and black birch trees. Pine, hemlock, and some types of oak trees are found on the higher parts of this habitat. Lower to the ground are huckleberry, teaberry, blueberry, and hawthorn and other plants. The wildflowers in this habitat include wild onion and wild strawberries. In the Nescopeck Creek watershed, pitch pine – scrub oak forests occur on Arbutus Peak and several barren areas in the southern part of watershed. In this type of forest, pitch pine, scrub oak, black oak and chestnut oak are the main trees. Bracken fern, teaberry, black chokeberry, blueberry, and huckleberry are the most common shrubs in this habitat.