This analysis was limited to examining one species per each of these three genera, due to its breadth. These data are in agreement with the karyotypic analysis of Acosta, et al., which examined 41 populations of 5 genera (21 species) within the Nicotianeae tribe of Solanaceae (later reorganized by into several new groups, including the supergeneric group Petunieae by Olmstead and Migid, el at 2008). This study found 2n=2x=18 (x=9) for "F. imbricata", "F. densa", and "F. denudata", "Calibrachoa" sp. as well as "Petunia" species examine. They hypothesize x=7 to have been the basal number for Solanales, and the x=12 to have derived from the tetraploid level, followed by tetraploid reduction. Within the groups surveyed, this methodology does not give rise to the same phylogenetic tree overall as that of Olmstead and Migid, but for Fabiana and sister genera, it is in agreement. While the agreement of the two chloroplast analyses with the karyotyic analysis is valuable, current data does not permit the detailed phylogenetic analysis of Fabiana below the genus level.