New attempts were made to create a Huguenot colony in the New World, again at the instigation of Coligny, this time in French Florida from 1562 to 1565, under Jean Ribault and René de Laudonnière. From 1568, Villegagnon became the representative of the Order of Malta at the French Court. The next year, in 1569, he published in Paris a new controversy about the eucharist, entitled ""De Consecratione, mystico sacrificio et duplici Christi oblatione"". Villegagnon became Commander of the Order of Malta Commandery in Beauvais, where he died on 9 January 1571, aged about 60. The Catholic André Thévet, who had accompanied him on the first trip to Brazil, published in 1572 a description of the Brazil adventure and an attack on the Protestants, in his ""Cosmographie Universelle"". The Protestant Jean Léry would respond to it in 1578 with his ""Histoire d'un voyage faict en la terre du Brésil"". In his book, "Brazil, A Land of the Future", Austrian writer Stefan Zweig describes the colorful character of Villegaignon:  On 2012 French-Portuguese-Brazilian TV series "Rouge Brésil"/"Vermelho Brasil" (Red Brazil), on the history of France Antarctique, Villegaignon (spelled Villegagnon) is portrayed by Swedish actor Stellan Skarsgard. A street in the Maltese city of Mdina is named after Villegaignon. He appears as an important supporting character in "The Course of Fortune" by Tony Rothman (J. Boylston, 2015), a novel that concerns the events leading to the Great Siege of Malta, 1565. He is a supporting character in Dorothy Dunnett's novel "The Disorderly Knights", the third volume of her Lymond Chronicles series. The knight is featured in "Eight Pointed Cross" by Marthese Fenech (BDL 2011), a historical novel that climaxes with the Ottoman Siege of 1551. Villegaignon will return in its sequel, which culminates in the Great Siege of Malta, 1565.