The tunnel lies on a level section of canal. To the east are Camden Locks, the first three of twelve locks through which the canal descends to Limehouse Basin, from which Limehouse Lock provides access to the Thames. After passing under the A5205 road bridge, there is a right-angled bend after the locks, with the stub of Cumberland Basin to the left. The route then follows the northern edge of Regent's Park, which houses London Zoo. There is a long wooded cutting, before the canal skirts a housing estate built on the site of the former Marylebone goods yard. It then passes through Eyre's Tunnel, which is often known as Lisson Grove Bridge, and into the eastern portal of Maida Hill Tunnel. The tunnel runs underneath Aberdeen Place on which stands the renowned Crocker's Folly Hotel, so called because it was reputed to have been built in anticipation of the arrival of the railway which went to Marylebone instead. After that it passes beneath Edgware Road to emerge at the western portal with its restaurant above. The section between the tunnel mouth and Little Venice is tree-lined, with fine Regency houses on both sides. There is a junction with the Paddington Arm, leading to Paddington Basin, and the main line of the canal continues westwards to Old Oak Common, where Eurostar trains are stabled, and Wormwood Scrubs park and prison. The canal through the tunnel is navigable by wide-beam boats, with a beam of , a draught of , a length of  and headroom of . The tunnel is from Paddington Junction, and from Hampstead Road Locks, a pair of locks which begin the fall to the Thames, and home to the Camden Canal Centre. There are a number of trip boats which operate on the section between Little Venice and Camden Locks, passing through the tunnel and calling at the zoo, and the tunnel is only wide enough for one way traffic. Boats are therefore required to give way if another boat is approaching in the opposite direction. In 2016, the Canal & River Trust provisionally opened access to non-powerfed craft such as kayaks, packrafts and canoes.