The predicted promoter region is 1116 bp long and located on chromosome 1 on the minus strand and extends from 46535401- 46536515. ElDorado through Genomatix was used to analyze the top 20 transcription factor binding sites within the promoter. TMEM275's RNA levels are very high at around 11 weeks of gestation within the intestines. Some other notable peaks include the lungs, kidneys, and adrenal tissues at 10, 16, and 20 weeks, respectively. It has also been found that of the 20 human tissues tested, RNA was notably present within fetal brain tissue. Further testing on tissue types lead to the discovery that TMEM275 may have tissue-specificity with the testis, the brain, and the prostate. Along with the thyroid and ovaries. TMEM275 is predicted to be within the plasma membrane or the endoplasmic reticulum's membrane. Various PTMs were analyzed for association with TMEM275. This includes looking for the presence of a SUMO-motif, acetylation sites, the presence of signal peptides, and any O-GlcNAc site and N-myristylation predictions. There are no known paralogs for TMEM275. No homologs or homologous domains exist within TMEM275. A total of 105 organisms are found to have orthologs with the human TMEM275 genes, all of which are a part of the Teleostomi, or jawed vertebrates, clade. Of the 105 organisms that have an orthologous TMEM275: 49 are mammals, 27 are birds, 3 are turtles, 3 are lizards, 3 are amphibians, 19 are bony fishes, and 1 is an alligator. The group with the most similar sequences by percent identity was unsurprisingly Mammalia. The percent identities of those orthologous proteins in relation to humans ranged from 52.1% to 70.9%. The group that had the orthologous TMEM275, but was the least similar was Actinopterygii or Bony Fishes. Their percent similarities ranged from 38.5% to 47.6%. Percent similarities were found by conducting a pairwise analysis of each orthologous protein within each species against the human protein. TMEM275 seems to have appeared 435 MYA within the Bony fishes or Actinopterygii. There were no known protein interactions for TMEM275. TMEM275 has no known link to medical disease.