This is an extremely important discovery to the scientific community as this optimum Nitrogen reduction in 92 °C is a total of 28 °C higher than the previously recorded nitrogen reduction of the methanogen, "Methanothermococcus thermolithotrophicus". Before the discovery of "Methanocaldococcus sp." FS406-22, "Methanothermococcus thermolithotrophicus was" the most thermophilic microorganism, fixing nitrogen at 64 °C. This particular discovery has potential to reveal a much wider range of conditions for life not only, in the seafloor biosphere but also, other ecosystems that are nitrogen deficient"." Looking further into this methanogen, it was found that the 16S rRNA gene of "Methanocaldococcus sp." FS406-22, is 99% similar to that of "Methanocaldococcus jannaschii" DSM 2661. This odd 1% divergence between FS406-22 and "M. jannaschii" is due to a previous separation of ancestral genes. The revelation of a hyperthermophilic, diazotrophic, archaea (strain FS406-22) may be due to the evolutionary history of nitrogenous. The phylogenetic analysis of nitrogenous and chlorophyll iron proteins suggests that an ancestral iron protein duplicated and diverged into "nifH" and "anfH" genes. This divergence of genes is estimated to have happened before the separation of the Domains bacteria and methanogenic archaea. Strain FS406-22 contains a functional "nifH" gene which is able to encode dinitrogenase reductase whereas "M. jannaschii" does not possess this gene, explaining the 1% divergence between the two species.