The formed hydrazine or imine could subsequently be converted by the enzyme hydroxylamine oxidase to dinitrogen gas, and the reducing equivalents produced in the reaction are required to combine NO or HNO and ammonium or to reduce nitrite to NO. Environmental genomics analysis of the species "Candidatus" "Kuenenia stuttgartiensis", through a slightly different and complementary metabolism mechanism, suggested NO to be the intermediate instead of hydroxylamine (Figure 4). However, this hypothesis also agreed that hydrazine was an important intermediate in the process. In this pathway (Figure 4), there are two enzymes unique to anammox bacteria: hydrazine synthase (hzs) and hydrazine dehydrogenase (hdh). The HZS produces hydrazine from nitric oxide and ammonium, and HDH transfer the electrons from hydrazine to ferredoxin. Few new genes, such as some known fatty acid biosynthesis and S-adenosylmethionine radical enzyme genes, containing domains involved in electron transfer and catalysis have been detected. Anammox microorganisms can also directly couple NO reduction to ammonia oxidation, without the need for nitrite supply. Another, still unexplored, reaction mechanism involves anaerobic ammonium oxidation on anodes of bio-electrical systems. Such systems can be microbial fuel cells or microbial electrolysis cells. In the absence of dissolved oxygen, nitrite, or nitrate, microbes living in the anode compartment are able to oxidize ammonium to dinitrogen gas (N2) just as in the classical anammox process. At the same time, they unload the liberated electrons onto the anode, producing electrical current. This electrical current can be used either directly in fuel cell mode or for hydrogen and methane gas production in electrolysis mode. While there is no clarity on the reaction mechanism behind, one hypothesis is that nitrite, nitrate, or dinitrogen oxide play a role as intermediates. However, since the process occurs at very low electrochemical potentials, other, more speculative, reaction mechanisms seem possible as well. Until now, ten anammox species have been described, including seven that are available in laboratory enrichment cultures. All have the taxonomical status of Candidatus, as none were obtained as classical pure cultures. Known species are divided over five genera: (1) "Kuenenia", represented by "Kuenenia stuttgartiensis", (2) "Brocadia" (three species: "B. anammoxidans", "B. fulgida", and "B. sinica"), (3) "Anammoxoglobus" (one species: "A. propionicus", (4) "Jettenia" (one species: "J. asiatica", and (5) "Scalindua" (four species: "S. brodae", "S. sorokinii", "S. wagneri", and "S. profunda" Representatives of the first four genera were enriched from sludge from wastewater treatment plants; "K. stuttgartiensis", "B. anammoxidans", "B. fulgida", and "A. propionicus" were even obtained from the same inoculum.