The bulk of the funding, went to algae research and development, while the rest went toward ethanol infrastructure. However, projections for future costs of petroleum are a moving target. DOE expects petroleum costs to remain relatively flat over the next 20 years. Algal biodiesel is expensive in comparison and might not be able to compete with such cheap petroleum prices. National Renewable Energy Laboratory scientists isolated around 3,000 algae species. Fifty-one varieties were characterized as potential high-value strains, but fewer than half of those remain. A few hundred strains were in storage at the University of Hawaii, which for years, every two months, transferred, “passaged”, them to new nutrient-rich test tubes. When a National Science Foundation grant ran out in 2004, maintaining the collection became impractical. A review released earlier this year found that more than half the genetic legacy had been lost. 23 of the 51 strains that were extensively studied during the program survive. The losses to the rest of the algal cultures have been even worse. “The really bloody shame is that of those 3,000, there are maybe 100 to 150 strains that remain at the University of Hawaii,” said Al Darzins, who heads up the resurgent algal biofuels research program at the National Renewable Energy Laboratory. During the Carter Administration in the mid-1970s, all energy-related offices were brought together under the newly formed Department of Energy. The hierarchy between the DOE and the Aquatic Species Program was as follows:        Dillagi (1999 film)  Dillagi is a 1999 Indian Hindi drama film co-produced and directed by Sunny Deol and also starring Sunny Deol alongside his real-life brother Bobby Deol and Urmila Matondkar. The film is noted for being the first time Sunny Deol directed a film and also the first time he acted alongside his brother Bobby. This pairing was repeated in "" (2002), "Apne" (2007), "Heroes" (2008), "Yamla Pagla Deewana" (2011), "Yamla Pagla Deewana 2" (2013), "Poster Boys" (2017) and "" (2018). Rajvir (Bobby Deol) and Ranvir (Sunny Deol) are devoted brothers. They along with their parents leave their village in Punjab to move to Mumbai. When their mother passes away, Ranvir takes on the responsibility of looking after his younger brother. After some hardship Ranvir manages to turn everything around and he becomes really wealthy.