Typically, safe working environments around sterilization equipment is achieved with electrochemical sensors capable of measuring in the parts per billion and low parts per million levels. These sensors are typically inexpensive and limited to ambient conditions. Moreover, HPV electrochemical sensors are often located near the sterilization equipment to detect possible leaks during the sterilization cycle. In 2014, Advanced Sterilization Products (ASP), sovaldi the manufacturer of the Sterrad hydrogen peroxide gas plasma sterilizer, issued a letter to hospital risk managers warning them that hydrogen peroxide residues may be found in the sterilization load. HPV being present in the sterilization load, could lead the accidental exposure of hospital staff. Monitoring hydrogen peroxide levels inside of the sterilization chamber during the sterilization cycle can be challenging. Technical issues such as condensation, vacuum, and high concentration have prevented many sensing technologies such as electrochemical sensors from providing real time monitoring of H2O2 concentration. Under these conditions, optical methods such as spectroscopy can be used to ensure that lethal concentrations of H2O2 are achieved in the sterilization chamber.