I am really thankful for your reply. Indeed, I never use any batch size, I am training the ANN in Matlab without any batch size. just feed all training data in one time. But, firstly I pass all 3000 samples, and another time feed in 3 steps of 1000, I expect that in the third step (reach 3000 training data) the sensitivity ,specificity, AUC, be the same as the first experiment, but it is lower :(.