_:n809225216e464e0aacbbd53cc8344c64xb2
<http://schema.org/text>
"Hello das33 , \n Using an ISTD&#39;s response to calibrate other compounds is referred to as Relative ISTD in Quant and Unknowns Analysis. In Quant it is enabled in the method&#39;s Global Setup. \n \n In Quant this will only work for defined targets and cannot be used to calculate an estimated amount for any peak, but Unknowns Analysis can be used for this workflow. \n Set up your UA file as usual and analyze. After the components are detected, right click on your ISTD and choose the option to Set Hits as ISTDs. \n \n Assign a concentration in the next dialog. \n \n Then in your method on the Target Match tab change the Estimation to Relative ISTD Estimation. \n \n Reanalyze your samples and you will now have an Estimated Conc."
<https://community.agilent.com/technical/mass-spectrometry-software/f/mass-spectrometry-software-user-forum/9027/gc-ms-masshunter-quantitative-analysis---use-calibration-for-all-peaks>