_:n2c25de449b1846b584bba0b61491602fxb2
<http://schema.org/text>
"<p>Hello <a href=\"/members/das33\" data-contentid=\"cd1064f342a54ec1a6e1fd15ef007e7e\" data-contenttypeid=\"e9ed411860ed4f2ba0265705b8793d05\" class=\"ui-contentpeek internal-link view-user-profile\">das33</a>&nbsp;,</p>\n<p>Using an ISTD&#39;s response to calibrate other compounds is referred to as Relative ISTD in Quant and Unknowns Analysis. In Quant it is enabled in the method&#39;s Global Setup.</p>\n<p><img style=\"max-height:480px;max-width:640px;\" src=\"/resized-image/__size/1280x960/__key/communityserver-discussions-components-files/152/pastedimage1727273350960v1.png\" alt=\" \" /></p>\n<p>In Quant this will only work for defined targets and cannot be used to calculate an estimated amount for any peak, but&nbsp;Unknowns Analysis can be used for this workflow.</p>\n<p>Set up your UA file as usual and analyze. After the components are detected, right click on your ISTD and choose the option to Set Hits as ISTDs.</p>\n<p><img style=\"max-height:480px;max-width:640px;\" src=\"/resized-image/__size/1280x960/__key/communityserver-discussions-components-files/152/pastedimage1727274157591v2.png\" alt=\" \" /></p>\n<p>Assign a concentration in the next dialog.</p>\n<p><img style=\"max-height:480px;max-width:640px;\" src=\"/resized-image/__size/1280x960/__key/communityserver-discussions-components-files/152/pastedimage1727274197542v3.png\" alt=\" \" /></p>\n<p>Then in your method on the Target Match tab change the Estimation to Relative ISTD Estimation.</p>\n<p><img style=\"max-height:480px;max-width:640px;\" src=\"/resized-image/__size/1280x960/__key/communityserver-discussions-components-files/152/pastedimage1727274233398v4.png\" alt=\" \" /></p>\n<p>Reanalyze your samples and you will now have an Estimated Conc.</p>\n<p><img style=\"max-height:480px;max-width:640px;\" src=\"/resized-image/__size/1280x960/__key/communityserver-discussions-components-files/152/pastedimage1727274304339v5.png\" alt=\" \" /></p><div style=\"clear:both;\"></div>"
<https://community.agilent.com/technical/mass-spectrometry-software/f/mass-spectrometry-software-user-forum/9027/gc-ms-masshunter-quantitative-analysis---use-calibration-for-all-peaks>