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"reaction buffer; target DNA servers as template for reaction; nucleotriphosphate bases dATP, dCTP, dGTP, dTTP; two 20-30 base oligonucleotide primers - usually 100 to 1000 bases from each other (must know DNA sequence of target DNA); thermostable DNA polymerase (tag) - not denatured at 95C, derived from a thermostable bacteria."
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