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"Intended Use The MBS EBV VCA IgA Antibody ELISA Test Kit has been designed for the determination of specific IgA antibodies against EBV VCA in serum and plasma. For research use only, not for use in diagnostic procedures. Principle of the Test The MBS EBV VCA IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). EBV VCA antigen is bound on the surface of the microtiter strips. Diluted unknowns are pipetted into the wells of the microtiter plate. A binding between the IgA antibodies of the serum and the immobilized EBV VCA antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgA peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm.   MBS494276 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Epstein-Barr IgA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards render a theoretical kit detection range in biological research samples containing Epstein-Barr IgA. The ELISA analytical biochemical technique of the MBS494276 kit is based on Epstein-Barr IgA antibody-Epstein-Barr IgA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Epstein-Barr IgA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Epstein-Barr IgA. Appropriate sample types include undiluted human body fluids and/or tissue homogenates, secretions such as Serum and plasma. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%)."^^<http://www.w3.org/2001/XMLSchema#string>
<https://www.mybiosource.com/prods/ELISA-Kit/Human/Epstein-Barr-IgA/datasheet.php?products_id=494276>