Terry Wogan and Natasha Kaplinsky hosted the annual fundraising event which has raised more than £17.2m.
Liberty X began the fundraising with a live performance of official Children in Need single A Night to Remember.
Prime minister Tony Blair appeared alongside Dead Ringers impressionist Jon Culshaw as part of the festivities.
Status Quo performed at RAF Brize Norton, in Oxfordshire, as did a number of acts including Girls Aloud.
A performance by Madonna was one of the highlights of the star-studded event, which lasted for seven hours.
The public dug deep into their pockets throughout the 26th annual fund-raising event.
One of the highlights was a performance of Queen's Bohemian Rhapsody by a group comprising of BBC news readers.