A west London tram system will still go ahead despite opposition by one of the boroughs affected by the network.
Work on the system is due to start in 2009
The £650m project, which would run from Shepherd's Bush to Uxbridge, was unanimously rejected by Ealing Council.
Labour admitted its support for the scheme was a major factor in its losing the council to the Conservatives earlier this month.
Transport for London said planning would continue regardless of the support of the councils involved.
A TfL spokesman said: "The West London Tram will be a massive investment, boosting the economy and making travel much easier in this part of London.
"TfL will continue to listen to the boroughs and local residents as we progress this scheme."
The spokesman said there was a limit to how many buses can run in the area and a tram service would "greatly enhance public transport".
A public inquiry is expected to be held before any scheme could go ahead, with the final decision resting with the transport secretary.
Ealing's Labour group has now joined the Conservatives in opposing the scheme.
Ealing Council leader Jason Stacey has written to the leaders of the other two councils to persuade them to oppose the scheme.
The project has been unpopular among some residents who fear it will increase congestion and affect trade.
Last year, a public consultation on the tram scheme in the three boroughs concerned showed 59% of people opposed it.
TfL had said previously it hoped to start work on the system in 2009 and to have the first trams running in 2013.