바이러스 단백질체학 및 대사체학 질량분석법 - Thermo Fisher Scientific
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protein glycosylation workflow
The surface of the virus envelope is covered with proteins, specifically glycoproteins, which are involved in the process of binding to the host cells for viral entry. Glycan diversity on these proteins are used by the virus to evade immune system response. Surface glycans have conformational flexibility that provides conformational dynamics to the virus, which can help shield drug binding sites. In order to get more detail on the type of glycosylation and the sites where it occurs (site occupancy) on viral surface glycoproteins, a glycoproteomics strategy can be used. Glycoproteomics can provide information on the sites of glycosylation and glycan compositions. Glycopeptide workflows involve sample preparation and enrichment, mass spectrometry analysis, data analysis and interpretation. Below is a short list of recommended products, but we invite you to explore more product information and resources on glycopeptide analysis on our <anchor>protein glycosylation workflow</anchor> page.
Protein Glycosylation | Thermo Fisher Scientific - US
Protein Glycosylation \| Thermo Fisher Scientific - US

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- Protein Glycosylation

# Protein Glycosylation

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[‹Proteomics Mass Spectrometry Workflows](/us/en/home/industrial/mass-spectrometry/proteomics-mass-spectrometry/proteomics-mass-spectrometry-workflows.html)

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## A sweet revolution in glycopeptide analysis

Posttranslational modification (PTM) of proteins with glycan groups to form glycoproteins is a common biological motif. Glycans are involved in a wide variety of biological and physiological processes, including recognition and regulatory functions, cellular communication, immunological defense, gene expression, and cell growth and development. Aberrant glycosylation of proteins is connected with cell immortalization, transformation, and metastasis. Due to its many biological functions, glycosylation is one of the most-studied PTMs of eukaryotic cell proteins.

[Contact us](/us/en/home/global/forms/industrial/proteomics-protein-mass-spectrometry-contact-us.html)

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On this page


- [Recommended products for protein glycosylation](#recommended-products)
- [Protein glycosylation workflow](#protein)

- [Featured glycan and glycopeptide analysis white paper](#featured)
- [Related resources and support](#resources)

* * *

Recommended products for protein glycosylation


### Enrich sample concentration, reduce complexity

Thermo Scientific Pierce Glycoprotein Isolation Kits, [Concanavalin A (ConA)](/order/catalog/product/89804) and [Wheat Germ Agglutinin (WGA)](/order/catalog/product/89805), enable the isolation of glycoproteins at the protein level and from complex mixtures such as serum, tissue, and cultured cell lysates. These complete kits contain the immobilized lectins, binding and wash buffers, as well as the columns required to process up to 10 mg of total protein.

### Streamline glycoproteomics workflows

Reversed-phase separation on Thermo Scientific low flow systems is an integral component of glycoproteomics workflows and combines seamlessly with Thermo Scientific Orbitrap mass spectrometers. The Thermo Scientific Vanquish Neo UHPLC System is the system of choice for glycoproteomics. The [Vanquish Neo UHPLC System](/us/en/home/industrial/chromatography/liquid-chromatography-lc/hplc-uhplc-systems/vanquish-neo-uhplc-system.html) combines an unrivaled degree of innovation to deliver 24/7 reproducible separations of complex mixtures at maximum performance for a variety of high-sensitivity LC-MS workflows

### Go beyond today's discovery

Today’s cutting-edge research pushes LC-MS to its limits. Obtaining high confidence insights to enable accurate resolution of subtle differences and avoid costly dead-ends is needed faster than ever before. The [Thermo Scientific Orbitrap Ascend Tribrid mass spectrometer](/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-tribrid-mass-spectrometers/orbitrap-ascend-tribrid-mass-spectrometer.html) surpasses these limits with new innovations that deliver the ultimate flexibility to expand experimental scope and architectural advancements to improve parallelization of experimental methods. One system provides maximum insights, so you productively go beyond today’s discovery.

### Realize fast and accurate glycopeptide analysis

The [Thermo Scientific Orbitrap Eclipse Tribrid mass spectrometer](/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-systems/orbitrap-lc-ms/orbitrap-tribrid-mass-spectrometers/orbitrap-eclipse-tribrid-mass-spectrometer.html) is HRAM-capable and offers multiple dissociation techniques (CID, HCD, ETD, EThcD, and UVPD) for thorough glycopeptide characterization. The system's intelligent data acquisition strategies simplify workflows while improving duty cycle and dynamic range. The Orbitrap Eclipse MS is also capable of synchronous precursor selection (SPS) MS3 acquisition with intelligent searching, supporting multiplexed quantification of glycopeptides from various biological samples. This workflow is only available on tribrid mass spectrometers.

### Simplify glycopeptide identification and characterization

Glycopeptide data interpretation is challenging because it requires both peptide sequencing and characterization of the glycosylation site(s) and overall glycan composition. [Thermo Scientific Proteome Discoverer Software](/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/multi-omics-data-analysis/proteome-discoverer-software.html) (2.0 and higher) offers Byonic™ Software from Protein Metrics as a node within its architecture. Byonic Software uses HCD data to determine peptide sugar composition, while corresponding ETD/EThcD data elucidate the peptide backbone. This results in an accurate portrayal of the peptide sequence, its site(s) of glycosylation, and its glycan composition.

[Top](#top)

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## Protein glycosylation workflow

To elucidate glycopeptide localization and composition, a combination of high-resolution accurate-mass (HRAM) mass spectrometry and multiple dissociation techniques is required. Such versatility is needed for thorough glycopeptide characterization. HCD or CID fragmentation provides glycan composition information, while ETD and EThcD is used to sequence the peptide and determine glycosylation sites.

Click image to enlarge

[Top](#top)

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## Featured protein glycosylation white paper

### [Orbitrap Fusion Mass Spectrometer for Glycan and Glycopeptide Analysis](https://assets.thermofisher.com/TFS-Assets/CMD/Reference-Materials/wp-64385-orbitrap-ms-glycan-glycopeptide-wp64385-en.pdf)

Read how the functionalities of this innovative instrument combine to produce a significant performance improvement for standard glycomics and glycoproteomics experiments. Additionally, the unique tribrid architecture makes a wide array of novel acquisition experiments possible facilitating workflows that were previously inaccessible with previous generation platforms.

[Download now](https://assets.thermofisher.com/TFS-Assets/CMD/Reference-Materials/wp-64385-orbitrap-ms-glycan-glycopeptide-wp64385-en.pdf)

### [Boost selectivity and enhance your proteomics workflow with the FAIMS Pro Duo Interface](/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html)

High-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) can be used to optimize ion selectivity and reduce chemical backgrounds during mass spectrometry assays. FAIMS selectivity successfully prevents interferences from co-eluting compounds and increases signal to noise ratios by 100-fold or higher.

[Learn more](/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html) [Watch free webinar](/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-accessories/field-asymmetric-ion-mobility-spectrometry-faims.html#videos)

[Top](#top)

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## Related Learning Centers  [More Learning Centers ›](/us/en/home/technical-resources/learning-centers.html)

- [Protein Biology ›](/us/en/home/life-science/protein-biology/protein-biology-learning-center.html)

[Resource Library ›](/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library.html)
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Technology Overview ›](/us/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/mass-spectrometry-technology-overview.html)
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Application Areas ›](/us/en/home/industrial/mass-spectrometry/mass-spectrometry-learning-center/mass-spectrometry-applications-area.html)

Resources


- [Comparison and Optimization of First and Second Generation Quadrupole Dual Cell Linear Ion Trap Orbitrap MS for Glycopeptide Analysis](https://assets.thermofisher.com/TFS-Assets/CMD/posters/PN-64756-Glycopeptides-ASMS2016-PN64756-EN.pdf)
- [Top Four Things to Know about Glycan Analysis by Mass Spectrometry](http://planetorbitrap.com/library?t=QTEzMjFlODg0OWE1NWYzYw==&keywords=A1321#tab:keywords)
- [Fragmentation Patterns of EThcD Spectra of Phosphopeptides and Glycopeptides](http://planetorbitrap.com/library?t=QTEzMDllODg0OWE1NWYzYw==&keywords=A1309#tab:keywords)

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