In this study, the investigators will examine whether a low carbohydrate diet will change hepatic lipid content and mitochondrial flux in humans. Before and after a low carbohydrate diet intervention, rates of hepatic mitochondrial flux will be assessed using positional isotopomer analysis of 3-13C-lactate during a 3 hour basal period. Rates of whole body ketogenesis will be determined using [13C4]β-hydroxybutyrate, and whole body glucose metabolism will be measured using [2H7]glucose. Body composition will be determined with bioelectrical impedance; hepatic lipid content will be measured with MRS. Whole-body oxidation of lipids, carbohydrates and protein will be determined using indirect calorimetry.
The purpose of this study is to examine whether a low carbohydrate diet decreases hepatic lipid content and changes hepatic mitochondrial flux in humans.