PATIENTS AND METHODS~Protocol Outline:~The Study:~Study site:~Ain Shams University Maternity Hospital.~Study Design:~A prospective Cohort study.~Study setting:~The study will be conducted at Ain Shams University Maternity Hospital.~Study population:~The study will include all women planned to undergo IVF due to unexplained infertility.~Methodology:~The study will be approved by the hospital ethics committee.~All included women will undergo the following:~History taking.~General and abdominal examination.~Vaginal examination and assessment~Pelvic ultrasound.~Routine laboratory investigations including complete blood picture, hematocrit, liver and kidney function tests and coagulation profile.~Preoperative senior anesthetist assessment.~Hysteroscopy.~All patients will undergo IVF.~Serum 25(OH)D samples will be collected within1 week before oocyte retrieval.~Whole blood specimens will be collected in serum separator tubes using acceptable medical techniques to avoid hemolysis.~Ultrasound-guided fresh embryo transfer will be performed on day 5 after fertilization.~clinical pregnancy will be confirmed by observation of intrauterine sac visible on ultrasound performed 4-5 weeks after embryo transfer.~Measurement of Outcomes~serum 25 (OH) D levels.~Number of oocyte.~Most studies put the optimal number of retrieved oocytes between 10 and 15.~The implantation rate:~Calculated as the number of gestational sacs observed by ultrasound divided by the number of embryos transferred, multiplied by 100.~Number of gestational sac.
All patients will undergo in vitro fertilization.~Serum 25 (OH) D samples will be collected within1 week before oocyte retrieval.~Whole blood specimens will be collected in serum separator tubes using acceptable medical techniques to avoid hemolysis.~Ultrasound guided fresh embryo transfer will be performed on day 5 after fertilization.~clinical pregnancy will be confirmed by observation of intrauterine sac visible on ultrasound performed 4-5 weeks after embryo transfer.