Sections of the tumor tissue will be HE stained.~Sections of the tumor tissue will be stained with an uPAR antibody for immunohistochemistry.~Immunohistochemical staining against cytokeratin which colors cancer cells will be made to assist the observer in the evaluation of uPAR microexpression pattern.~A semiqualitative scale corresponding to the level of uPAR expression will be made.~Quantitative polymerase chain reaction will be used to validate the immunohistochemistry uPAR expression pattern.~An electronic scoring system (digital pathology) will be trained to access the expression of uPAR in both tumor- and normal tissue.
The aim of this study is to investigate urokinase Plasminogen Activator Receptor (uPAR) microexpression in gastroesophageal cancer (adenocarcinomas) both qualitatively and semi-quantitatively and to evaluate if it offers a possibility for future imagining purposes.