The primary objective is to establish the differences in vasomotor response of a DCB and DES treated artery by measurement of the percentage change in coronary surface area pre and post acetylcholine administration. This will be measured with incremental doses of acetylcholine at levels both proximally and distally to the site of treatment Also is to see how far endothelial dysfunction may propagate from an interventional site by measuring drug induced dilatation/constriction as in response to acetylcholine administration. This vasomotor response will be measured in the target vessel at extended distances from the treated segment.~To achieve a meaningful comparison, 34 patients will be recruited of which 17 patients would form either arm of the study having had either a DCB or DES.~The study will be performed at the Norfolk and Norwich University Hospital and patients will be enrolled prior to a second procedure. This may occur immediately after their stent or drug coated balloon treatment or if they re-attend hospital in the following recruitment period and this may include patients for a variety of different indications.~Patients who have a scheduled, clinically indicated procedure will be eligible to be recruited as participants. They must also fulfil the inclusion criteria and not be affected by any of the exclusion criteria.~A member of the research team will provide the Patient Information Sheet to potential participants and confirm that they would be happy to be contacted subsequently to discuss the proposed research. The subsequent contact will be undertaken by a clinical research fellow, who is both a member of the Norfolk and Norwich University Hospital NHS clinical team as well as the research team, prior to their subsequent visit to discuss the study, answer any questions and confirm if they fulfil the inclusion criteria. In view of Covid-19, to reduce in hospital exposure, we might instead opt to send the patient information to the eligible patients by post, and follow with a telephone call to confirm receipt.~Method: Patients will be prepared in the usual way as per clinical practice for their procedure. They will attend via a standardised case pathway depending on the indication for repeat angiography (staged procedure versus re-presentation acutely for another reason such as chest discomfort). There are a series of routine checks performed to ensure that the patient is on appropriate medications. For the purpose of the study, anti-anginal medications will need to be stopped for 72 hours prior to the procedure. The anti-anginal medications that would require cessation include:~Cardio selective Beta 1 selective blockers (Atenolol, Bisoprolol, Carvedilol, Esmolol, Metoprolol and Nebivolol )~Diltiazem~Nicorandil~Isosorbide Nitrate preparations~Ranolazine The particular medication regime will vary on a patient to patient basis and the appropriateness of this and nature of drug cessation will be discussed at the enrolment process and patients will be given clear instruction on adjustments to their regimen.~Coronary Angiography will be performed as per common clinical practice. At the beginning of the study portion of the procedure, there will be two orthogonal angiographic views taken of the treated segment which will then be denoted as the reference views for the rest of the procedure (used after every administration of agonist).~Acetylcholine (ACh) will be diluted into 0.9% normal saline at different dilutions (to a total volume of 5mL). NB - these solutions of Acetylcholine are usable within a two-hour period, beyond this time a new solution should be used. When reassessing the previously treated artery, an infusion of intracoronary acetylcholine will be administered via the guiding catheter. The first ACH infusion (low dose) will be 0.182micrograms/mL (10^-6mol/L) given at a rate of 1Ml/min for two minutes by a mechanical infusion pump (50). After the infusion, intracoronary contrast will be injected and images acquired.~If there are no adverse features, the investigator can proceed with the subsequent increments with each infusion to complete, followed by a five-minute interval. The next increments would be:~medium dose 1.82mcg/ml (10^-5mol/L) ACH in 5ml normal saline - given over 20 seconds.~high dose 18.2mcg/ml (10^-4mol/l) ACH in 5ml normal saline - given over 20 seconds.~Then after a further five minutes - a bolus dose of GTN (400mcg) will be administered over 20 seconds and then a further contrast acquisition taken within two minutes. Also see Appendix C for lab flow chart.~Once these images have been taken, the guide catheter will be removed the procedure will then be completed in the usual way. The patient with then be moved to the appropriate recovery area as per the standard protocol following PCI procedures.
Use of intracoronary acetylcholine to investigate endothelial function in coronary arteries treated with either drug coated balloon angioplasty of drug eluting stents.