MSCs in passage-2 culture will be washed with PBS and detached with trypsin/EDTA (0.25%). After that, the cells will be suspended at a density of 20×106 cells/ 2 ml normal saline and loaded into 3 ml sterile syringes.~The cells should be infused within 2 hours of release. Tests and follow up are to be monthly.
Autologous bone marrow-derived mesenchymal cells will be injected into patients diagnosed with premature ovarian failure