Purpose:~To evaluate PGTai results for embryos cultured in sequential versus single step media.~Method:~Injected oocytes will divided between Continuous Single Culture-NX media (CSC- NX) with 10% SSS the first group (Irvine, USA) and the other group will be cultured in sequential cleavage and blastocyst media with 10% SPS (SAGE, Denmark).~All blastocytes with 4BB quality and better according to Gardner's criteria 1999 will go through PGTA procedures with PGTai confirmation.
A prospective cohort study undergoing ICSI at a private fertility center in Egypt