Written informed consent obtained from every participant.~Detailed history taking including onset, course, duration of the disease, aggravating factors and received medications~VASI score assessed in every patient.~Patients photographed, divided into two groups early & late cases and 3 mm punch biopsies will be taken from perilesional and non lesional skin.~Biopsies processed and stained for CD8 & CD69 in cases of early group, & late group and controls by immunofluorescence.
This study aims to detect the presence and diversity of Tissue resident memory T cell populations in early and late cases of generalized non-segmental Vitiligo.