The investigators plan to follow the child every three months for a year. Data were collected every 3 months for a total of 5 follow-up data collections (baseline, 3 months, 6 months, 9 months, and 12 months). The contents of each follow-up included questionnaire collection, anthropometric measurement, body composition measurement, visual acuity measurement, blood pressure measurement, venous blood extraction, etc., as well as family questionnaire, school questionnaire, community hospital and project hospital intervention doctor questionnaire. In the first, third and fifth follow-up visits, 2-3 ml of venous blood should be collected.~Myopia and activity records: including nighttime lighting, wake-sleep cycles, and movement. As well as family questionnaires, school questionnaires, community hospitals and project hospital intervention doctor questionnaires. All inspection items are operated by trained and qualified doctors and professional health technicians. The inspection will be arranged in the school, and the whole process is the same as the regular physical examination in the school.~The child required 3 venous blood collections, urine and saliva collections during the year (at the initial stage and at the 3rd follow-up, respectively).~Blood sample testing: 2-3ml of venous blood (about 1/2 of the bottle cap of mineral water) is drawn each time for blood routine and blood biochemistry. The blood process is safe. Blood samples include: blood biochemical indicators (fasting blood glucose (FBG), insulin, total cholesterol (TCHO), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein cholesterol (HDL-C) )).~Hormone testing: Collect saliva samples in the morning and purchase ELISA kits to measure levels of thyroid hormones, estradiol and testosterone.~Urine sample testing: use polypropylene urine cups and urine collection tubes (without PAEs plasticizers) to collect 10 mL of urine from the research subjects, and reserve the sample to collect the first excretion after getting up in the morning, before breakfast and before exercising. Before urinating in the middle of the urine, students should be asked to clean the urethral opening and surrounding skin with warm water to avoid the contamination of secretions. The collected urine samples were uniformly stored in a 5mL centrifuge tube and stored in a -20°C refrigerator. Urine PAEs and their metabolites: Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to detect seven PAEs metabolites in students' urine: monomethyl phthalate (MMP), phthalic acid Monoethyl ester (MEP), mono-n-butyl phthalate (MBP), mono(2-ethylhexyl) phthalate (MEHP), mono(2-ethyl-5-phthalate) Hydroxyhexyl) ester (MEHHP), mono(2-ethyl-5-oxohexyl) phthalate (MEOHP).~The content of the family and student questionnaires will include general conditions of children, general conditions of parents and families, as well as daily diet, physical activity and sleep. The name of the child will only be used for tracking and will be deleted after one year of observation to ensure that the information of the child and participants will not be leaked. All members of the project team have signed a confidentiality agreement, and all information in the questionnaire will not be leaked to the outside world.
Based on the RE-AIM framework, this project intends to integrate the prevention and control measures of myopia and obesity, and establish a set of key suitable technical packages and guarantee systems for the comorbidity-common-cause-common prevention of myopia and obesity in children and adolescents. Hetongzhou District selected 24 intervention schools (about 20,000) and 12 control schools (about 10,000) to apply the application, integrate the resources of the family-school-community-medical institution, and establish a comprehensive combination of myopia and obesity in children and adolescents In the prevention and control demonstration area, a one-year intervention study was conducted to evaluate the comprehensive intervention effect of myopia and obesity in children and adolescents in the demonstration area, and to promote it through the regional monitoring system in Beijing. This project is the first time to establish a district-level comprehensive prevention and control demonstration area for children and adolescents with myopia and obesity that can be promoted, including key appropriate technology packages and promotion and application strategies. Healthy Beijing 2030 plan laid the foundation.