Itaconate is a covalent inhibitor of the Mycobacterium tuberculosis isocitrate lyase
Kwai, Brooke X. C., Collins, Annabelle J., Middleditch, Martin J., Sperry, Jonathan, Bashiri, Ghader, Leung, Ivanhoe K. H.
Itaconate is a mammalian antimicrobial metabolite that inhibits the isocitrate lyases (ICLs) of Mycobacterium tuberculosis. Herein, we report that ICLs form a covalent adduct with itaconate through their catalytic cysteine residue. These results reveal atomic details of itaconate inhibition and provide insights into the catalytic mechanism of ICLs.