Antimicrobial Peptides against Multidrug-Resistant <i>Pseudomonas aeruginosa</i> Biofilm from Cystic Fibrosis Patients.
Ben Hur D, Kapach G, Wani NA, Kiper E, Ashkenazi M, Smollan G, Keller N, Efrati O, Shai Y.
Lung infection is the leading cause of morbidity and mortality in cystic fibrosis (CF) patients and is mainly dominated by <i>Pseudomonas aeruginosa</i>. Treatment of CF-associated lung infections is problematic because the drugs are vulnerable to multidrug-resistant pathogens, many of which are major biofilm producers like <i>P. aeruginosa</i>. Antimicrobial peptides (AMPs) are essential components in all life forms and exhibit antimicrobial activity. Here we investigated a series of AMPs (d,l-K<sub>6</sub>L<sub>9</sub>), each composed of six lysines and nine leucines but differing in their sequence composed of l- and d-amino acids. The d,l-K<sub>6</sub>L<sub>9</sub> peptides showed antimicrobial and antibiofilm activities against <i>P. aeruginosa</i> from CF patients. Furthermore, the data revealed that the d,l-K<sub>6</sub>L<sub>9</sub> peptides are stable and resistant to degradation by CF sputum proteases and maintain their activity in a CF sputum environment. Additionally, the d,l-K<sub>6</sub>L<sub>9</sub> peptides do not induce bacterial resistance. Overall, these findings should assist in the future development of alternative treatments against resistant bacterial biofilms.