en en en en cs en en fi en en en en en en en en en en gl en en fi en gl lt en en en en en gl gl gl pt en en gl gl gl pt en en en en en en en en en pt pt es en en en gu en es it es es en pt ceb it fr de ml en en en en eo en en en en en en en en en en gl en fr en en en en en fr en en pt
Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage
FAQ on Copyright FAQ on Copyright
More information at INFOguias UDC More information at INFOguias UDC
Grupo de Investigación en Nanotoxicoloxía e Toxicoloxía Xenética (NANOTOXGEN)
GI - NANOTOXGEN - Artigos
Grupo de Investigación en Nanotoxicoloxía e Toxicoloxía Xenética (NANOTOXGEN)
GI - NANOTOXGEN - Artigos
JavaScript is disabled for your browser. Some features of this site may not work without it.
Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage
Use this link to cite
Except where otherwise noted, this item's license is described as Atribución 4.0 Internacional
GI-DICOMOSA - Artigos [19]
GI - NANOTOXGEN - Artigos [7]
Show full item record
Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage
Valdiglesias, V.; Fernández-Bertólez, N.; Lema-Arranz, C.; Rodríguez-Fernández, R.; Pásaro, E.; Reis, A.T.; Teixeira, J.P.; Costa, C.; Laffon, B. Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage. Nanomaterials 2021, 11, 1930. https://doi.org/10.3390/nano11081930
[Abstract] Metal oxide nanoparticles (NPs) have a wide variety of applications in many consumer products and biomedical practices. As a result, human exposure to these nanomaterials is highly frequent, becoming an issue of concern to public health. Recently, human salivary leucocytes have been proposed as an adequate non-invasive alternative to peripheral blood leucocytes to evaluate genotoxicity in vitro. The present study focused on proving the suitability of salivary leucocytes as a biomatrix in the comet assay for in vitro nanogenotoxicity studies, by testing some of the metal oxide NPs most frequently present in consumer products, namely, titanium dioxide (TiO₂), zinc oxide (ZnO), and cerium dioxide (CeO₂) NPs. Primary and oxidative DNA damage were evaluated by alkaline and hOGG1-modified comet assay, respectively. Any possible interference of the NPs with the methodological procedure or the hOGG1 activity was addressed before performing genotoxicity evaluation. Results obtained showed an increase of both primary and oxidative damage after NPs treatments. These data support the use of salivary leucocytes as a proper and sensitive biological sample for in vitro nanogenotoxicity studies, and contribute to increase the knowledge on the impact of metal oxide NPs on human health, reinforcing the need for a specific regulation of the nanomaterials use.
All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects
UNIVERSIDADE DA CORUÑA. Servizo de Biblioteca.    DSpace Software Copyright © 2002-2013 Duraspace - Send Feedback