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Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton - Tutkimusportaali - Converis - Jyväskylän yliopisto
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Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton (2021)
Salmi, P., Mäki, A., Mikkonen, A., Pupponen, V.-M., Vuorio, K., & Tiirola, M. (2021). Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton. Boreal Environment Research, 26, 17-27. http://www.borenv.net/BER/archive/pdfs/ber26/ber26-017-027.pdf
Julkaisun kaikki tekijät tai toimittajat: Salmi, Pauliina; Mäki, Anita; Mikkonen, Anu; Pupponen, Veli-Mikko; Vuorio, Kristiina; Tiirola, Marja
Lehti tai sarja: Boreal Environment Research
Julkaisun avoin saatavuus: Avoimesti saatavilla
Julkaisukanavan avoin saatavuus: Kokonaan avoin julkaisukanava
Julkaisu on rinnakkaistallennettu (JYX): https://jyx.jyu.fi/handle/123456789/76018
The smaller the phytoplankton, the greater effort is required to distinguish individual cells by optics-based methods. Flow cytometry is widely applied in marine picophytoplankton research, but in freshwater research its role has remained minor. We compared epifluorescence microscopy and flow cytometry in assessing the composition, abundance and cell sizes of autofluorescent picophytoplankton in epilimnia of 46 Finnish lakes. Phycocyaninrich picocyanobacteria were the most dominant. The two methods yielded comparable total picophytoplankton abundances, but the determination of cell sizes, and thus total biomasses, were on average an order of magnitude higher in the microscopy results. However, flow cytometry yielded higher cell sizes when applied on small-celled cultured algae. Our study demonstrated that both epifluorescence microscopy and flow cytometry are useful methods in assessing abundances of phycocyanin-rich and phycoerythrin-rich picocyanobacteria and eukaryotic picophytoplankton in lakes. However, accurate determination of cell size and biomass remain challenges for microscopy and especially for flow cytometry.
YSO-asiasanat: plankton; syanobakteerit; mikrolevät; mikroskopia; fluoresenssimikroskopia; biomassa (ekologia); laskentamallit
1172 Ympäristötiede (117 Maantiede ja ympäristötieteet)
1183 Kasvibiologia, mikrobiologia, virologia (118 Biotieteet)
Bio- ja ympäristötieteiden laitos
Hankkeet, joissa julkaisu on tehty
Micro-RIP Functional analysis of uncultivated microbes using radioisotope probing
Original data for article: Comparison of epifluorescence microscopy and flow cytometry in counting freshwater picophytoplankton (2019) Salmi, Pauliina; et al.; 10.17011/jyx/dataset/66278
Viimeisin päivitys 2022-20-09 klo 13:27
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