Diplopod limb buds first differ visibly from normal limb buds by their abnormally thick and long apical ectodermal ridges. Diplopodia usually delays the embryonic growth of cartilage, bone, and tendon by two days, particularly in the limbs. Other characteristics frequently seen in diplopodia mutants are shortened wings and legs, short upper beak, and smaller embryo size. Many embryos reach the final embryonic stage but then are unable to hatch, though, diplopod embryos can occasionally hatch and mature.Single diplopodia mutations produce wide ranges of phenotypes sharing these general characteristics and can even affect individual chickens left and right legs differently. The ranges of phenotypes produced by the different mutations also overlap broadly, so diplopodia mutations sometimes need to be distinguished by their phenotype distributions. Dr. Ursula Abbott has extensively studied the phenotypes and descriptions of the first four diplopodia mutations and has ranked them from least severe to most severe: dp-3, dp-1, dp-4, dp-2.Diplopodia-1
This autosomal mutation gives homozygotes duplicated wing tips and feet in front of the usual structures, as in the experimentally induced diplopodia described above. However, unlike the experimentally manipulated embryos, these embryos show no change in shh expression. The embryos express hoxd-11, hoxd-12, hoxd-13, Bmp-2, and Fgf-4 along the whole edge of each limb bud, even though these genes are normally only expressed at the posterior edge.Diplopodia-2
This is an autosomal trait that gives a very extreme diplopod phenotype, with up to eight toes on a foot. In some embryos, the two outer front toes on each foot are joined together.
Vaginal lubrication is a naturally produced fluid that lubricates a 
vagina. Vaginal lubrication is always present, but production increases significantly near ovulation and during sexual arousal in anticipation of sexual intercourse. Vaginal dryness is the condition in which this lubrication is insufficient, and sometimes artificial lubricants are used to augment it. Without sufficient lubrication, sexual intercourse can be painful. The vaginal lining has no glands, and therefore the vagina must rely on other methods of lubrication. Plasma from vaginal walls due to vascular engorgement is considered to be the chief lubrication source, and the Bartholins glands, located slightly below and to the left and right of the introitus (vaginal opening), also secrete mucus to augment vaginal-wall secretions. Near ovulation, cervical mucus provides additional lubrication. Vaginal discharge
Composition
The lubricating fluid can vary in consistency, texture, taste, color, and odor, depending on sexual arousal, the phase of the menstrual cycle, the presence of an infection, certain drugs, genetic factors, and diet.Vaginal fluid is slightly acidic and can become more acidic with certain sexually transmitted diseases. The normal pH of vaginal fluid is between 3.8 and 4.5, contrasting with male semen which is typically between 7.2 and 7.8 (neutral pH is 7.0).During arousal, vaginal lubrication, also sometimes called "arousal fluid", is produced. This is clear, thin, and slippery. It typically only lasts up to an hour. Colloquially, this is referred to as "getting wet". Production
The human vagina is serviced by nerves that respond to vasoactive intestinal polypeptide (VIP).