Diagnosis
With colonoscopy it is possible to detect small ulcers of between 3–5mm, but diagnosis may be difficult as the mucous membrane between these areas can look either healthy or inflamed. Trophozoites may be identified at the ulcer edge or within the tissue, using immunohistochemical staining with specific anti-E. histolytica antibodies.Asymptomatic human infections are usually diagnosed by finding cysts shed in the stool. Various flotation or sedimentation procedures have been developed to recover the cysts from fecal matter and stains help to visualize the isolated cysts for microscopic examination. Since cysts are not shed constantly, a minimum of three stools are examined. In symptomatic infections, the motile form (the trophozoite) is often seen in fresh feces. Serological tests exist, and most infected individuals (with symptoms or not) test positive for the presence of antibodies. The levels of antibody are much higher in individuals with liver abscesses. Serology only becomes positive about two weeks after infection. More recent developments include a kit that detects the presence of amoeba proteins in the feces, and another that detects ameba DNA in feces. These tests are not in widespread use due to their expense.Microscopy is still by far the most widespread method of diagnosis around the world. However it is not as sensitive or accurate in diagnosis as the other tests available. It is important to distinguish the E. histolytica cyst from the cysts of nonpathogenic intestinal protozoa such as Entamoeba coli by its appearance.
Tolnaftate (INN) is a synthetic thiocarbamate used as an anti-fungal agent that may be sold  without medical prescription in most jurisdictions. It is supplied as a cream, powder, spray, liquid, and liquid aerosol. Tolnaftate is used to treat fungal conditions such as jock itch, athletes foot and ringworm. Mechanism
Although the exact mechanism of action is not entirely known, it is believed to inhibit squalene epoxidase, an important enzyme in the biosynthetic pathway of ergosterol (a key component of the fungal cell membrane) in a similar way to terbinafine. Uses
Tolnaftate has been found to be generally slightly less effective than azoles when used to treat tinea pedis (athletes foot). It is, however, useful when dealing with ringworm, especially when passed from pets to humans. Side effects
Side effects that may occur include:
allergic reactions like:
skin rash
itching or hives
swelling of the face, lips, or tongue
inflammation, redness, or pain at the affected areaLess severe side effects include:
dry skin
mild skin irritation, burning, or itching at the affected area

See also
Liranaftate, a similar thiocarbamate antifungal

References
External links
Medlines entry for tolnaftate