The PCR assay utilizes primers based on the 16S rRNA gene base sequences of human and rodent strains of S. moniliformis (forward primer, 5′ GCT TAA CAC ATG CAA ATC TAT 3′ and reverse primer, 5′ AGT AAG GGC CGT ATC TCA 3′). These primers exhibit 100% complementarity to S. moniliformis ATCC 14674T and S. moniliformis ANL 370-1. The PCR assay generates a 296-bp product which upon treatment with BfaI restriction enzyme, leads to the generation of three distinct fragments (128, 92, and 76 bp), which are specific to S. moniliformis. Hence, this assay can be used to detect S. moniliformis with great accuracy. Prevention
Although rare, the disease is certainly seeing a spike in the number of incidences because of various reasons. The most important among them is the fact that rodents are increasingly finding their way into our homes either as a pet or as a pest. In fact, the number of children who are affected by rat bite fever has been specifically on the rise. Therefore, wild rats should not be brought home and if there is an infestation, appropriate measures for extermination must be undertaken to prevent the disease from spreading. Treatment
The bacteria are susceptible to a number of antibiotics. They are: cephalosporins, carbapenems, aztreonam, clindamycin, erythromycin, nitrofurantoin, bacitracin, doxycycline, tetracycline, teicoplanin, and vancomycin. However, the data suggests that treatment with erythromycin can be less efficient. Intravenous penicillin G (400000–600000 IU/day) should be administered for 7 days and then a dosage of oral penicillin must be prescribed.
In Middle Eastern countries, it is not acceptable to burp out loud in public, and one should silence ones burp, or at least attempt to do so.Despite virtually no scientific research on the subject, small online communities exist for burping as a sexual fetish. Online, both men and women of any sexual orientation anecdotally report some attraction to burping, with what appears to be psychological and/or behavioural overlaps with other sexual fetishes including body inflation, feederism, vorarephilia, and farting fetishes. Anecdotally, the loudness aspect appears to be an important element to burp fetishists. Despite being a rather uncommon fetish, it continues to follow a general well-known pattern of sexual behaviour where hearing influences sexual arousal and response, noting that "it is the noise made rather than the action itself that appears to be what is sexualized and/or interpreted by the fetishist as sexually pleasurable and arousing". Infants
Babies are likely to accumulate gas in the stomach while feeding and experience considerable discomfort (and agitation) until assisted. Burping an infant involves placing the child in a position conducive to gas expulsion (for example against the adults shoulder, with the infants stomach resting on the adults chest) and then lightly patting the lower back. Because burping can cause vomiting, a "burp cloth" or "burp pad" is sometimes employed on the shoulder to protect clothing. Contest
The Guinness World Record for the loudest burp is 109.9 dB, set by Paul Hunn at Butlins Bognor Regis, United Kingdom, on 23 August 2009.