	From an evolutionary perspective BotIT6 is linked to both the depressant and the α group. BotIT6 is similar to depressant insect toxins, but also has similarities to α type and excitatory toxins. BotIT6 shares 58-66% of its amino-acid sequence with depressant insect toxins, and 24-34% with alfa type and excitatory toxins. In addition, it shares functional characteristics with all three groups. This has led to the hypothesis that BotIT6 could be an ancestral depressant toxin.