question: The present study aims to evaluate whether influenza and pneumococcal vaccinations are associated with positive nasopharyngeal swab (NPS) testing to detect SARS-CoV-2 Data from the Italian cross-sectional web-based survey (EPICOVID19), based on a self-selection sample of individuals aged ≥18, were considered The probability of a positive SARS-CoV-2 NPS test result as a function of influenza or anti-pneumococcal vaccination was evaluated using multivariable logistic regression, stratifying analysis by age (&lt;65 years, ≥65 years) From April 2020, 170,731 individuals aged &lt;65 years and 28,097 ≥65 years filled out the EPICOVID19 questionnaire Influenza and anti-pneumococcal vaccinations were received, respectively, by 16% and 2% of those &lt;65 years, and by 53% and 13% of those ≥65 years SARS-CoV-2 NPS testing was reported by 6680 participants Anti-pneumococcal and influenza vaccinations were associated with a decreased probability of a SARS-CoV-2 NPS positive test in the younger participants (OR = 0 61, 95% CI 0 41–0 91;OR = 0 85, 95%CI 0 74–0 98;respectively) A significantly lower probability of a positive test result was detected in the individuals ≥65 years who received anti-pneumococcal vaccination (OR = 0 56, 95%CI 0 33–0 95) These results need to be confirmed by further investigations, but they are relevant given the probable coexistence of influenza, bacterial infections, and COVID-19 over the coming autumn–winter season