question: Ultra-high frequency (UHF) multiple input multiple output (MIMO) passive radio frequency identification (RFID) systems have attracted the attention of many researchers in the last few years. The system modeling and theoretical performance analysis of these systems have been well investigated and revealed in many studies, yet the system prototype and the corresponding experimental results are scarce. In this study, measurements of a 1 × 2 × 1 UHF passive RFID system, including a MIMO UHF passive RFID tag prototype and its corresponding software-defined radio-based reader, taken in a microwave anechoic chamber, are presented. The experimental results are compared with theoretical values and computer simulations. The overall results demonstrate the consistency and the feasibility of UHF MIMO passive RFID systems.