question: This study aims to develop a prototype of a virtual laboratory (VL) application through Biology, Technology, Engineering, and Mathematics (BTEM) that is feasible and practical to use to facilitate virtual practicum activities on the concept of bacteria The final product application generated from this research is expected to help students understand the concepts of bacterial The research and development method used in this study is the Van De Akker model with four stages is Preliminary research, prototyping stage, summative evaluation, and systematic reflection and documentation stages This research has been carried out in two phases, preliminary research and prototype stages The next phase is implementation which will take place in the school after the covid-19 pandemic is over Adobe Flash CS 6 and Construct 2 were used to create this application This research has only reached the prototype development stage and limited trials by experts The expert assessment sheet and analysis of the results of the limited trial are used as research instruments The results showed that the prototype of the product made still needs to be improved, especially in the aspects of software engineering and visual communication design and linguistic aspects