question: Background: The outbreak of coronavirus disease 2019 (COVID-19) has rapidly spread all over the world. The specific information about immunity of non-survivors with COVID-19 is scarce. We aimed to describe the clinical characteristics and abnormal immunity of the confirmed COVID-19 non-survivors. Methods: In this single-centered, retrospective, observational study, we enrolled 125 patients with COVID-19 who were died between Jan, 13 and Mar 4, 2020 from Renmin Hospital of Wuhan University. 414 randomly recruited patients with confirmed COVID-19 who were discharged from the same hospital during the same period served as control. Demographic and clinical characteristics, laboratory findings and chest computed tomograph results at admission, and treatment were collected. The immunity-related risk factors associated with in-hospital death were detected. Results: Non-survivors were older than survivors. More than half of non-survivors was male. Nearly half of the patients had chronic medical illness. The common signs and symptoms at admission of non-survivors were fever. Non-survivors had higher white blood cell (WBC) count, more elevated neutrophil count, lower lymphocytes and platelete count, raised concentration of procalcitonin and C-reactive protein (CRP) than survivors. The levels of CD3 + T cells, CD4 + T cells, CD8 + T cells, CD19 + T cells, and CD16 + 56 + T cells were significantly decreased in non-survivors when compared with survivors. The concentrations of immunoglobulins (Ig) G, IgA and IgE were increased, whereas the levels of complement proteins (C)3 and C4 were decreased in non-survivors when compared with survivors. Non-survivors presented lower levels of oximetry saturation at rest and lactate. Old age, comorbidity of malignant tumour, neutrophilia, lymphocytopenia, low CD4 + T cells, decreased C3, and low oximetry saturation were the risk factors of death in patients with confirmed COVID-19. The frequency of CD4 + T cells positively correlated with the numbers of lymphocytes and the level of oximetry saturation, whereas CD4 + T cells were negatively correlated with age and the numbers of neutrophils. Conclusion: Abnormal cellular immunity and humoral immunity were considerable in non-survivors with COVID-19. Neutrophilia, lymphocytopenia, low CD4 + T cells, and decreased C3 were the immunity-related risk factors predicting mortality of patients with COVID-19.