question: To evaluate the effect and outcome by Shokuiku and nutrition education, it is need to develop the reliability and validity questionnaire for behaviors and interests in healthy eating habits for Japanese adolescents. Our aim was to develop the reliability and validity questionnaire about them for Japanese adolescents. Interests in healthy eating habits for Japanese adolescents were modified Japanese questionnaire of consciousness in dietary life (JQCDL) for university students developed by Hamaguchi et al. The surveys were carried out at two public junior high schools and 381 students in second year participated. Reliability of JQCDL-adolescent form (JQCDL-AF) was examined by factor analysis and calculation of Cronbach's α. Validity of JQCDL-AF was examined by divergent of gender and by experimental intervention which investigated the changed scores from lunchbox to school lunch. High score of JQCDL-AF showed more interests in healthy eating habits. From histogram and factor analysis, four questions were excluded from JQCDL. We chose two subscale factors to form the domains: reduction unhealthy foods when eating out or snacking (4 items), and meal manners and meal preparation (7 items). Cronbach's α of two subscales and all items (11 questions) were 0.845, 0.826, and 0.860, respectively. The total JQCDL-AF scores in girls were significantly higher than those of boys. Moreover, the total JQCDL-AF scores 1-mo after introducing school lunches increased significantly from the lunchbox. Therefore, the JQCDL-AF could evaluate interests in healthy eating habits in Japanese adolescents.