text: An experiment observing chilling stress on the tropical plant species, Spathiphyllum wallisii by scientists Maria Segura and Maria Quiles, showcased varying responses by chlororespiratory pathways when different parts of the plant were chilled at 10 degrees Celsius.
  Segura and Quiles noticed that when the roots of the plant were subjected to low temperatures (10 degrees Celsius), the level of chlororespiratory molecules (NADPH and PTOX) slightly varied when compared to the level of NADPH and PTOX within the controlled plant. However, when the stem alone was cooled at 10 degrees Celsius, then the molecules NADPH, NDH and PTOX increased in amount as a result of reduced PS I activity. Segura and Quiles then compared this result, by subjecting only the leaves of the plant to 10 degrees Celsius. They noticed that this had caused the PS II activity to stop, and thus inhibit the process of photosynthesis. The lack in photosynthetic activity, in combination with the incline in NADPH and PTOX molecules, then triggered chlororespiratory pathways to begin chemical energy synthesis.
  Furthermore, Segura and Quiles also noted that the simultaneous chilling of the leaves and heating of the roots (whilst the plant is under illumination), can cause the slowing and eventual inhibition of the ETC in PS II.  This then led to an over-reduction in the PQ pool., which ultimately stimulated chlororespiration.
  Segura and Quiles utilised the Fluorescence imaging technique to determine the level of photosynthetic activity in the leaves of the plants. By discerning the percentage of photosynthesis efficiency, Segura and Quiles were able to determine the likelihood of triggering chlororespiratory pathways.  They noticed that the percentage of photosynthesis efficiency remained high in test subjects where:
  * only the leaves were chilled
  * only the stem was chilled
  * only the roots were chilled 
  This high percentage of photosynthesis efficiency meant that the chances of chlororespiration taking place are slim.  However, this was not true for the plant that underwent both stem chilling at 10 degrees Celsius and root heating at 24 degrees Celsius. The photosynthesis efficiency of this test subject was significantly lower when compared to the experimental control. This also indicated the inhibition of PS II activity which then caused chlororespiration to begin.
  Segura and Quiles also used an immunoblot analysis to deduce the effect of varying temperatures on different parts of the plant. Specifically, the immunoblot measures the amount of PTOX and NDH complex accumulated within the thylakoid membrane of the chloroplast organelle. An increase in NDH complex was evident in the plant where the stem was chilled at 10 degrees Celsius and the root heated at 24 degrees Celsius. Chlororespiration was stimulated in this plant.  Dissimilarly, the immunoblot analysis detected no variation in the levels NDH complex and PTOX molecules in test subjects where:
  * only the leaves were chilled
  * only the stem was chilled
  * only the roots were chilled
  These test subjects had similar concentrations of NDH and PTOX when compared to the concentration of NDH complex and PTOX molecules within the experimental control.