text: The chelating properties of aminopolycarboxylates can be engineered by varying the groups linking the nitrogen atoms so as to increase selectivity for a particular metal ion. The number of carbon atoms between the nitrogen and carboxyl group can also be varied and substituents can be placed on these carbon atoms. Altogether this allows for a vast range of possibilities. Fura-2 is noteworthy as it combines two functionalities: it has high selectivity for calcium over magnesium and it has a substituent which makes the complex fluorescent when it binds calcium. This reagent provides a means of determining the calcium content in intra-cellular fluid. Details concerning applications of the following examples can be found in the individual articles and/or reference. The aminopolycarboxylate nicotianamine is widespread in plants, where it is used to transport iron.