text: A typical optical transfection protocol is as follows:
  1) Build an optical tweezers system with a high NA objective
  2) Culture cells to 50-60% confluency
  3) Expose cells to at least 10 µg/ml of plasmid DNA
  4) Dose the plasma membrane of each cell with 10-40 ms of focussed laser, at a power of <100 mW at focus
  5) Observe transient transfection 24-96h later
  6) Add selective medium if the generation of stable colonies is desired