text: Depletion forces were first described by Sho Asakura and Fumio Oosawa in 1954. In their model, the force is always considered to be attractive. Additionally, the force is considered to be proportional to the osmotic pressure. The Asakura–Oosawa model assumes low macromolecule densities and that the density distribution, , of the macromolecules is constant.  Asakura and Oosawa described four cases in which depletion forces would occur. They first described the most general case as two solid plates in a solution of macromolecules. The principles for the first case were then extended to three additional cases.