text: RNA ladders composed of RNA molecular-weight size markers were initially developed by using the synthetic circle method to produce different-sized markers. This technique was improved upon by inventor Eric T. Kool to use circular DNA vectors as a method for producing RNA molecular-weight size markers. As referred to as the rolling circle method, the improvements of this technique stems from its efficiency in synthesizing RNA oligonucleotides. From the circular DNA template, single-stranded RNA varying in length from 4-1500 bp can be produced without the need for primers and by recycling nucleotide triphosphate. DNA can also be synthesized from the circular template, adding to this technique's versatility. In comparison to runoff transcription, the synthetic circle method produces RNA oligonucleotides without the runoff. In comparison to PCR, the synthetic circle method produces RNA oligonucleotides without the need for polymerase nor a thermal cycler. This method is also cost-efficient in its ability to synthesize grand amounts of product at a lower error rate than machine synthesizers.