text: Temperature is a strong controlling factor on biochemical reaction rates and biological activity. Optimal temperature varies across aquatic organisms as some organisms are more cold-adapted while others prefer warmer habitats. There are rare cases of extreme thermal tolerance in hypersaline antarctic lakes (e.g. Don Juan Pond) or hot springs (e.g. Fly Geyser); however, most lake organisms on Earth reside in temperatures ranging from 0 to 40 °C. Metabolic rates typically scale exponentially with temperature, however, the activation energy for primary productivity and respiration often differ, with photosynthesis having a lower activation energy than aerobic respiration. These differences in activation energies could have implications for net metabolic balance within lake ecosystems as the climate warms. For example, Scharfenberger et al. (2019) show that increasing water temperature resulting from climate change could switch lakes from being net autotrophic to heterotrophic due to differences in activation energy, however, the temperature at which they switch depends on the amount of nutrients available.