text: In a photobiological process, the hydrogen is produced using photosynthetic microorganisms (green microalgae and cyanobacteria) in photobioreactors. Some of these organisms produce hydrogen upon switching culture conditions; for example, Chlamydomonas reinhardtii produces hydrogen anaerobically under sulfur deprivation, that is, when cells are moved from one growth medium to another that does not contain sulfur, and are grown without access to atmospheric oxygen. Another approach was to abolish activity of the hydrogen-oxidizing (uptake) hydrogenase enzyme in the diazotrophic cyanobacterium Nostoc punctiforme, so that it would not consume hydrogen that is naturally produced by the nitrogenase enzyme in nitrogen-fixing conditions.  This N. punctiforme mutant could then produce hydrogen when illuminated with visible light.
  Another mutant Cyanobacteria, Synechocystis, is using genes of the bacteria Rubrivivax gelatinosus CBS to produce hydrogen. The CBS bacteria produce hydrogen through the oxidation of carbon monoxide. Researchers are working to implement these genes into the Synechocystis. If these genes can be applied, it will take some effort to overcome the problems of oxygen inhibition in the production of hydrogen, but it is estimated that this process can potentially yield as much as 10% solar energy capture. This makes photobiological research a very exciting and promising branch of the hydrogen production explorations. Still the problems of overcoming the short-term nature of algal hydrogen production are many and research is in the early stages. However, this research provides a viable way to industrialize these renewable and environmental friendly processes.