text: The chemical components of orcein were elucidated only in the 1950s by Hans Musso. The structures are shown below. A paper originally published in 1961, embodying most of Musso's work on components of orcein and litmus, was translated into English and published in 2003 in a special issue of the journal Biotechnic & Histochemistry (Vol 78, No. 6) devoted to the dye.  A single alternative structural formula for orcein, possibly incorrect, is  given by the National Library of Medicine  and Emolecules.
  Orcein is a reddish-brown dye, orchil is a purple-blue dye. Orcein is also used as a stain in microscopy to visualize chromosomes, elastic fibers, Hepatitis B surface antigens, and copper-associated proteins.
  Orcein is not approved as a food dye (banned in Europe since January 1977), with E number E121 before 1977 and E182 after. Its CAS number is . Its chemical formula is CHNO. It forms dark brown crystals. It is a mixture of phenoxazone derivates - hydroxyorceins, aminoorceins, and aminoorceinimines.