text: Add master mix which contains buffer, dNTP mix, MgCl, Taq polymerase and nuclease-free water to each PCR tube. Then add the necessary primer to the tubes. Next, place the PCR tubes in a thermal cycler for 30 cycles of the amplification program. This includes: denaturation, annealing, and elongation. The products of RT-PCR can be analyzed with gel electrophoresis.