text: Level P vectors are similar to level M constructs except that the BpiI sites are replaced by BsaI sites and the BsaI sites are replaced by BpiI sites. Several level M constructs with compatible fusion sites can be subcloned into a level P vector in one step. Theoretically, as many as 36 genes can be assembled in one construct using 6 parallel level M reactions (each required for assembly of 6 genes per level M construct) followed by one final level P reaction. In practice, fewer genes are usually assembled as most cloning projects do not require so many genes.  
  The structure of level M and P vectors is designed in a such as way that genes cloned in level P constructs can be further assembled in level M vectors. Repeated cloning in level M and P vectors forms a loop that can be repeated indefinitely to assemble progressively large constructs.