text: Recombination hotspots are DNA sequences that increase local recombination.  The HOT1 sequence in yeast is one of the most well studied mitotic recombination hotspots.  The HOT1 sequence includes an RNA polymerase I transcription promoter.  In a yeast mutant strain defective in RNA polymerase I the HOT1 activity in promoting recombination is abolished.  The level of RNA polymerase I transcription activity that is dependent on the promoter in the HOT1 sequence appears to determine the level of nearby mitotic recombination.