text: Vectorette PCR can develop a strategy to bring about PCR amplification that is unidirectional. Vectorette PCR comprises three main steps. The first step includes utilizing a restriction enzyme in order to accomplish digestion of the sample DNA. The DNA that is to be utilized for the purpose of investigation has to be capable of being digested by restriction enzymes that are appropriate for that gene otherwise the DNA fragments that form the general population cannot be created. After that is completed, a Vectorette library is brought together by ligating the Vectorette units to the appropriate DNA fragments which were previously digested. Ligation is the act of binding two things together. A Vectorette unit is only partially not completely double stranded with a mismatched section located in the center of the unit. The reason it is mismatched is to help it avoid Vectorette primers’ attempts at causing it to undergo first strand synthesis. By doing this any priming that is nonspecific is also avoided. This ligation brings together the vectorette which is double stranded and the ends of the restriction fragments which were previously made in the first step. By doing this, the known sequence which is used to prime the PCR reaction at one side is introduced while the other is primed on the genomic sequence which is already known to the user.  The third and last step has two parts to it. This is due to there being two primers, the initiating primer (IP) and the Vectorette primer (VP), that act in different stages. During the first part, the IP works on amplifying the primer extension while the VP remains hybridized with the product; thus, any background amplification is not carried out at this stage. However, this changes during the last and following part of PCR as the priming that is performed comes from both the IP and the VP.