text: Traditionally, the Cohn process incorporating cold ethanol fractionation has been used for albumin purification.  However, chromatographic methods for separation started being adopted in the early 1980s.  Developments were ongoing in the time period between when Cohn fractionation started being used, in 1946, and when chromatography started being used, in 1983.  In 1962, the Kistler & Nistchmann process was created which was a spinoff of the Cohn process. Chromatographic processes began to take shape in 1983. In the 1990s, the Zenalb and the CSL Albumex processes were created which incorporated chromatography with a few variations.
  The general approach to using chromatography for plasma fractionation for albumin is: recovery of supernatant I, delipidation, anion exchange chromatography, cation exchange chromatography, and gel filtration chromatography.
  The recovered purified material is formulated with combinations of sodium octanoate and sodium N-acetyl tryptophanate and then subjected to viral inactivation procedures, including pasteurisation at 60 °C.
  This is a more efficient alternative than the Cohn process for four main reasons:
  1) smooth automation and a relatively inexpensive plant was needed,
  2) easier to sterilize equipment and maintain a good manufacturing environment,
  3) chromatographic processes are less damaging to the albumin protein, and
  4) a more successful albumin end result can be achieved.
  Compared with the Cohn process, the albumin purity went up from about 95% to 98% using chromatography, and the yield increased from about 65% to 85%.  Small percentage increases make a difference in regard to  sensitive measurements like purity. There is one big drawback in using chromatography, which has to do with the economics of the process. Although the method was efficient from the processing aspect, acquiring the necessary equipment is a big task.  Large machinery is necessary, and for a long time the lack of equipment availability was not conducive to its widespread use.  The components are more readily available now but it is still a work in progress and will possibly be ready in the future to help the world.