text: Most equilibrium fractionations are thought to result from the reduction in vibrational energy (especially zero-point energy) when a more massive isotope is substituted for a less massive one. This leads to higher concentrations of the massive isotopes in substances where the vibrational energy is most sensitive to isotope substitution, i.e., those with the highest bond force constants.
  In a reaction involving the exchange of two isotopes, X and X, of element "X" in molecules AX and BX,
  each reactant molecule is identical to a product except for the distribution of isotopes (i.e., they are isotopologues). The amount of isotopic fractionation in an exchange reaction can be expressed as a fractionation factor:
   indicates that the isotopes are distributed evenly between AX and BX, with no isotopic fractionation.  indicates that X is concentrated in substance AX, and  indicates X is concentrated in substance BX.  is closely related to the equilibrium constant (K):
  where  is the product of the rotational symmetry numbers of the products (right side of the exchange reaction),  is the product of the rotational symmetry numbers of the reactants (left side of the exchange reaction), and  is the number of atoms exchanged.
  An example of equilibrium isotope fractionation is the concentration of heavy isotopes of oxygen in liquid water, relative to water vapor,
  At 20 °C, the equilibrium fractionation factor for this reaction is
  Equilibrium fractionation is a type of mass-dependent isotope fractionation, while mass-independent fractionation is usually assumed to be a non-equilibrium process.
  For non-equilibrium reactions, isotopic effects are better described by the GEBIK and GEBIF equations for transient kinetic isotope fractionation, which generalize non-steady isotopic effects in any chemical and biochemical reactions.