text: Microscope-based diagnostics are widely performed and served as a gold standard in histological analysis. However this procedure generally requires a series time-consuming lab-based procedures including fixation, paraffin embedment, sectioning, and staining to produce microscope slides with optically thin tissue slides (4–6 µm). While in developed regions histology is commonly used, people who live in areas with limited resources can hardly access it and consequently are in need for a low-cost, more efficient way to access pathological diagnosis. The main significance of MUSE system comes from its capacity to produce high-resolution microscopic image with subcellular features in a time-efficient manner with less costs and less lab-expertises requirements.
  With 280 nm deep UV excitation and simple but robust hardware design, MUSE system can collect fluorescence signals without the need for fluorescence filtering techniques or complex mathematical image reconstruction. It has potential for generate high quality images containing more information than microscope slides in terms of its 2.5 dimensional features. MUSE images have been validated with diagnostic values. The system is capable to produce images from various tissue type in different sizes, either fresh or fixed.