text: Restriction enzyme DNA assembly has cloning standards to minimize the change in cloning efficiency and the function of the plasmid, which can be caused by compatibility of the restriction sites on the insert and those on the vector.
  Golden Gate assembly's cloning standards have two tiers. First-tier Golden Gate assembly constructs the single-gene construct by adding in genetic elements such as promoter, open reading frames, and terminators. Then, second-tier Golden Gate assembly combine several constructs made in first-tier assembly to make a multigene construct. To achieve second-tier assembly, modular cloning (MoClo) system and GoldenBraid2.0 standard are used.