text: The choice of culture medium might affect the physiological relevance of findings from cell culture experiments due to the differences in the nutrient composition and concentrations. A systematic bias in generated datasets was recently shown for CRISPR and RNAi gene silencing screens (especially for metabolic genes), and for metabolic profiling of cancer cell lines. For example, a stronger dependence on ASNS (asparagine synthetase) was found in cell lines cultured in DMEM, which lacks asparagine, compared to cell lines cultured in RPMI or F12 (containing asparagine). Avoiding such bias might be achieved by using a uniform media for all screened cell lines, and ideally, using a growth medium that better represents the physiological levels of nutrients. Recently, such media types, as Plasmax and Human Plasma Like Medium (HPLM), were developed.