text: In order to simultaneously manipulate and image samples that exhibit fluorescence, optical tweezers can be built alongside a fluorescence microscope. Such instruments are particularly useful when it comes to studying single or small numbers of biological molecules that have been fluorescently labelled, or in applications in which fluorescence is used to track and visualize objects that are to be trapped.
  This approach has been extended for simultaneous sensing and imaging of dynamic protein complexes using long and strong tethers generated by a highly efficient multi-step enzymatic approach and applied to investigations of disaggregation machines in action.