text: Ribosomal RNA, or rRNA, forms the nucleic acid component of ribosomes. rRNA modifications take place in and around the peptidyl transferasecenter, the active site of the ribosome. Some modifications include pseudouridines, 2′-O-methylations on backbone sugars, and methylated bases. It is not well known what the biological effects of these modifications are on the rRNA molecule, but one hypothesis is that they help stabilize the structure and enhance the function of the ribosome, especially during ribosome formation. Moreover, these modifications may alter the chemical properties of the rRNA such that the correct tertiary structure is favored. 2-O-methylation prevents backbone hydrolysis; other noted modifications also seem to help with stabilizing rRNA secondary structures and preventing damage to rRNA strands. 2-O-methylation also helps to increase base stacking forces, stabilizing the secondary and tertiary structure of rRNA even further. Collectively, these modifications in rRNA are indispensable to ribosomal function.