text: The NASBA technique has been used to develop rapid diagnostic tests for several pathogenic viruses with single-stranded RNA genomes, e.g. influenza A,  zika virus, foot-and-mouth disease virus, severe acute respiratory syndrome (SARS)-associated coronavirus, human bocavirus (HBoV) and also parasites like Trypanosoma brucei.
  Recently, NASBA reaction with fluoresce, dipstick and next generation sequencing readout has been developed for COVID-19 diagnosis.