text: Total RNA is purified from the specimen of interest. Poly A messenger RNA is then purified from total RNA and subsequently translated into cDNA using a reverse transcription reaction. The cDNA produced from the mRNA is labeled using primers homologous to the spliced leader sequences of the organism. In a nine step PCR reaction the cDNAs are concurrently embedded with the BpmI restriction endonuclease site (though any class IIs restriction endonuclease may work) and a biotin label which are present in the primers. These tagged cDNAs are then cleaved 14 bp downstream from the recognition site using BpmI restriction endonuclease and blunt ended with T4 DNA polymerase. The fragments are further purified away from extraneous DNA material by using the biotin labels to bind them to a strepdavidin matrix.  They are then ligated to adapter DNA, in six separate reactions, containing six different restriction endonuclease recognition sites. These tags are then amplified by PCR with primers containing a mismatch changing the Bpm1 site to a Xho1 site. The amplicons are concatenated and ligated into a plasmid vector. The clonal vectors are then sequenced and mapped to the genome.