text: For electron backscattering diffraction microscopy, a flat polished crystalline specimen is usually placed inside the microscope chamber. The sample is tilted at ~70° from Scanning electron microscope (SEM) flat specimen positioning and 110° to the electron backscatter diffraction (EBSD) detector. Tilting the sample elongates the interaction volume perpendicular to the tilt axis, allowing more electrons to leave the sample providing better signal. A high-energy electron beam (typically 20 kV) is focused on a small volume and scatters with a spatial resolution of ~20 nm at the specimen surface. The spatial resolution varies with the beam energy, angular width, interaction volume, nature of the material under study, and, in transmission Kikuchi diffraction (TKD), with the specimen thickness; thus, increasing the beam energy increases the interaction volume and decreases the spatial resolution.
  The EBSD detector is located within the specimen chamber of the SEM at an angle of approximately 90° to the pole piece. The EBSD detector is typically a phosphor screen that is excited by the backscattered electrons. The screen is coupled to lens which focuses the image from the phosphor screen onto a charge-coupled device (CCD) or complementary metal–oxide–semiconductor (CMOS) camera.
  In this configuration, as the backscattered electrons leave the sample, they interact with the Coulomb potential and also lose energy due to inelastic scattering leading to a range of scattering angles (θ). The backscattered electrons form Kikuchi lines – having different intensities – on an electron-sensitive flat film/screen (commonly phosphor), gathered to form a Kikuchi band. These Kikuchi lines are the trace of a hyperbola formed by the intersection of Kossel cones with the plane of the phosphor screen. The width of a Kikuchi band is related to the scattering angles and, thus, to the distance d between lattice planes with Miller indexes h, k, and l. These Kikuchi lines and patterns were named after Seishi Kikuchi, who, together with , was the first to notice this diffraction pattern in 1928 using transmission electron microscopy (TEM) which is similar in geometry to X-ray Kossel pattern.
  The systematically arranged Kikuchi bands, which have a range of intensity along their width, intersect around the centre of the regions of interest (ROI), describing the probed volume crystallography. These bands and their intersections form what is known as Kikuchi patterns or electron backscatter patterns (EBSPs). To improve contrast, the patterns’ background is corrected by removing anisotropic/inelastic scattering using static background correction or dynamic background correction.