text: A custom DNA plasmid sequence can be created and replicated in various ways, but generally, all methods share the following processes:
  Plant transformation using plasmids begins with the propagation of the binary vector in E. coli. When the bacterial culture reaches the appropriate density, the binary vector is isolated and purified. Then, a foreign gene can be introduced. The engineered binary vector, including the foreign gene, is re-introduced in E. coli for amplification.
  The engineered binary factor is isolated from E. coli  and is introduced into Agrobacteria containing a modified (relatively small) Ti plasmid. This engineered Agrobacteria can be used to infect plant cells. The T-DNA, which contains the foreign gene, becomes integrated into the plant cell genome. In each infected cell, the T-DNA is integrated at a different site in the genome.
  The entire plant will regenerate from a single transformed cell, resulting in an organism with the transformed DNA integrated identically across all cells.