text: Laserspray Ionization (LSI) is a newer mass spectrometric technique commonly used with biomolecules, such as proteins. This method is similar to matrix-assisted laser desorption/ionization (MALDI) at atmospheric pressure in that it involves an analyte and matrix mixture. It also contains features from electrospray ionization, in which it produces a similar mass spectra.  The mechanism was initially thought to involve laser induced production of highly charge matrix/analyte clusters that upon evaporation of the matrix produces ions by the same mechanism as ESI. LSI's ability to ablate proteins at atmospheric pressure in order to form a multiple of charged ions with a mass resolution of 100,000 when coupled with a quadrupole orbitrap mass spectrometer. The advantages of using LSI includes a solvent-free ionization technique, fast data acquisition, simply to use, and the improved fragmentation through multiple charging.