text: * Overcoming the reusability challenge with the blots, can allow for the use of a particular oligonucleotide probe where the radioactive labeling is varied to study the degree of binding that mutants of the probe possess with proteins.
  * Oligonucleotides are not able to penetrate the gel of SDS-PAGE to bind to the proteins which stresses the importance of having the blotting step. However, it may not be needed if "protein-resolving-oligonucleotide-permeable" gels are created. With such gels, oligonucleotide probes of interest could enter the gels and bind to the proteins minimizing steps of the technique while keeping results in one place.
  * There is evidence that tissue-specific DNA binding proteins can be identified by use of southwestern blot mapping. Moreover, their sequence-specific binding allows the purification of the corresponding selectively bound DNA fragments and may improve protein-mediated cloning of DNA regulatory sequences.