text: The majority of circulating cfDNA molecules are fragments linked to nucleosomes, so they represent unique chromatin arrangements found in the nuclear genomes of the cells they originate from. In particular, open chromatin areas j, whereas genomic regions linked to nucleosomal complexes are often shielded from endonuclease activity. Several studies have identified specific chromatin fragmentomic characteristics that aid in informing tissue origins through cfDNA profiling. These features include:
  # Reduced sequencing coverage depth
  # Disruption of nucleosome positioning near transcription start sites (TSSs)
  # Length of cfDNA fragments