text: m6A-CLIP (crosslinking immunoprecipitation) and miCLIP (m6A individual-nucleotide-resolution crosslinking and immunoprecipitation) are UV-based sequencing techniques. These two methods activate crosslinking at 254 nm, fragments RNA molecules before immunoprecipitation with antibody, and do not depend on the incorporation of photoactivatable ribonucleosides - the antibody directly crosslinks with a base close (very predictable location) to the m6A site. These UV-based strategies uses antibodies that induces consistent and predictable mutational and truncation patterns in the cDNA strand during reverse-transcription that could be leveraged to more precisely locate the m6A site. Though both m6A-CLIP and miCLIP reply on UV induced mutations, m6A-CLIP is distinct by taking advantage that m6A alone can induce cDNA truncation during reverse transcription and generate single-nucleotide mapping for over ten folds more precise m6A sites (MITS, m6A-induced truncation sites), permitting comprehensive and unbiased precise m6A mapping. In contrast, UV-mapped m6A sites by miCLIP is only a small subset of total precise m6A sites. The precise location of tens of thousands of m6A sites in human and mouse mRNAs by m6A-CLIP reveals that m6A is enriched at last exon but not around stop codon.
  In m6A-CLIP and miCLIP, RNA is fragmented to ~20-80nt first, then the 254 nm UV-induced covalent RNA/m6A antibody complex was formed in the fragments containing m6A. The antibody was removed with proteinase K before reverse-transcription, library construction and sequencing. Remnants of peptides at the crosslinking site on the RNA after antibody removal, leads to insertions, truncations, and C to T mutations during reverse transcription to cDNA, especially at the +1 position to the m6A site (5’ to the m6A site) in the sequence reads.  		 	 	 		
  Positive sites seen using m6A-CLIP and miCLIP had high percent of matches with those detected using SCARLET, which has higher local resolution around a specific site, (see below), implicating m6A-CLIP and miCLIP has high spatial resolution and low false discovery rate. 
  miCLIP has been used to detect m6Am by looking at crosslinking-induced truncation sites at the 5’UTR.