text: A sample of cells, either derived from an in vitro cell culture or from an in vivo test subject is dispersed into individual cells and suspended in molten low-melting-point agarose at 37 °C. This mono-suspension is cast on a microscope slide. A glass cover slip is held at an angle and the mono-suspension is applied to the point of contact between the coverslip and the slide. As the coverslip is lowered onto the slide the molten agarose spreads to form a thin layer. The agarose is gelled at 4 °C and the coverslip removed.
  The agarose forms a matrix of carbohydrate fibres that encapsulate the cells, anchoring them in place. The agarose is considered to be osmotic-neutral, therefore solutions can penetrate the gel and affect the cells without cells shifting position.
  In an in vitro study the cells would be exposed to a test agent – typically UV light, ionising radiation, or a genotoxic chemical – to induce DNA damage in the encapsulated cells. For calibration, hydrogen peroxide is usually used to provide a standardized level of DNA damage.