text: Similar to hyperspectral data analysis, the pump–probe imaging data, known as a delay stack, has to be processed to obtain an image with molecular contrast of the underlying molecular species. Processing pump–probe data is challenging for several reasons – for example, the signals are bipolar (positive and negative), multi-exponential, and can be significantly altered by subtle changes in the chemical environment. The main methods for analysis of pump–probe data are multi-exponential fitting, principal component analysis, and phasor analysis.