text: Recently, a well-studied quorum quenching bacterial strain (KM1S) was isolated and its AHL degradation kinetics were studied using rapid resolution liquid chromatography (RRLC). RRLC efficiently separates components of a mixture to a high degree of sensitivity, based on their affinities for different liquid phases. It was found that the genome of this strain encoded an inactivation enzyme with distinct motifs targeting the degradation of AHLs.