text: CTCF insulators affect the expression of genes implicated in cell cycle regulation processes that are important for cell growth, cell differentiation, and programmed cell death (apoptosis). Two of these cell cycle regulation genes that are known to interact with CTCF are hTERT and C-MYC. In these cases, a loss of function mutation to the CTCF insulator gene changes the expression patterns and may affect the interplay between cell growth, differentiation and apoptosis and lead to tumourigenesis or other problems.
  CTCF is also required for the expression of tumour repressor retinoblastoma (Rb) gene and mutations and deletions of this gene are associated with inherited malignancies. When the CTCF binding site is removed expression of Rb is decreased and tumours are able to thrive.
  Other genes that encode cell cycle regulators include BRCA1, and p53, which are growth suppressors that are silenced in many cancer types, and whose expression is controlled by CTCF. Loss of function of CTCF in these genes leads to the silencing of the growth suppressor and contributes to the formation of cancer.
  The aberrant activation of insulators can modulate the expression of cancer-related genes, including matrix metalloproteinases involved in cancer cell invasion.