text: tRNA (also tRNA-like) splicing is another rare form of splicing that usually occurs in tRNA.  The splicing reaction involves a different biochemistry than the spliceosomal and self-splicing pathways.
  In the yeast Saccharomyces cerevisiae, a yeast tRNA splicing endonuclease heterotetramer, composed of TSEN54, TSEN2, TSEN34, and TSEN15, cleaves pre-tRNA at two sites in the acceptor loop to form a 5-half tRNA, terminating at a 2,3-cyclic phosphodiester group, and a 3-half tRNA, terminating at a 5-hydroxyl group, along with a discarded intron.  Yeast tRNA kinase then phosphorylates the 5-hydroxyl group using adenosine triphosphate.  Yeast tRNA cyclic phosphodiesterase cleaves the cyclic phosphodiester group to form a 2-phosphorylated 3 end.  Yeast tRNA ligase adds an adenosine monophosphate group to the 5 end of the 3-half and joins the two halves together.  NAD-dependent 2-phosphotransferase then removes the 2-phosphate group.