text: Quorum sensing is used by bacteria to form biofilms. Quorum sensing is used by bacteria to form biofilms because the process determines if the minimum number of bacteria necessary for biofilm formation are present. The criteria to form a biofilm is dependent on a certain density of bacteria rather than a certain number of bacteria being present. When aggregated in high enough densities, some bacteria may form biofilms to protect themselves from biotic or abiotic threats. Quorum sensing is used by both Gram-positive and Gram-negative bacteria because it aids cellular reproduction. Once in a biofilm, bacteria can communicate with other bacteria of the same species. Bacteria can also communicate with other species of bacteria. This communication is enabled through autoinducers used by the bacteria.
  Additionally, certain responses can be generated by the host organism in response to the certain bacterial autoinducers. Despite the fact that specific bacterial quorum sensing systems are different, for example the target genes, signal relay mechanisms, and chemical signals used between bacteria, the ability to coordinate gene expression for a specific species of bacteria remains the same. This ability alludes to the larger idea that bacteria have potential to become a multicellular bacterial body.
  Secondly, biofilms may also serve to transport nutrients into the microbial community or transport toxins out by means of channels that permeate the extracellular polymeric matrix (like cellulose) that holds the cells together. Finally, biofilms are an ideal environment for horizontal gene transfer through either conjugation or environmental DNA (eDNA) that exists in the biofilm matrix.
  The process of biofilm development is often triggered by environmental signals, and bacteria are proven to require flagella to successfully approach a surface, adhere to it, and form the biofilm. As cells either replicate or aggregate in a location, the concentration of autoinducers outside of the cells increases until a critical mass threshold is reached. At this point, it is energetically unfavorable for intracellular autoinducers to leave the cell and they bind to receptors and trigger a signaling cascade to initiate gene expression and begin secreting an extracellular polysaccharide to encase themselves inside.