text: This way of evaluation deals with agar-agar or gelatine containing semi-solid layers made prior to the experiment. Small wells are cut into the layer and filled with cells and the test substance. Cells can migrate towards the chemical gradient in the semi solid layer or under the layer as well. Some variations of the technique deal also with wells and parallel channels connected by a cut at the start of the experiment (PP-technique). Radial arrangement of PP-technique (3 or more channels) provides the possibility to compare chemotactic activity of different cell populations or study preference between ligands.
  Counting of cells: positive responder cells could be counted from the front of migrating cells, after staining or in native conditions in light microscope.