text: properties of the compounds according to the present invention are as follows : the structure of compound ( i ) has been determined mainly by nuclear magnetic resonance spectrum ( table i ), mass spectrum ( table 2 ) and other physico - chemical properties ( table 3 ). the minimum inhibitory concentration ( mic ) of compound ( i ) is shown in table 4 in comparison with that of spiramycin i . table 1__________________________________________________________________________carbon nuclear magnetic resonance spectrum compound no . carbon no . 1 2 3 4 5 6 7 8 9__________________________________________________________________________ 1 170 . 0 170 . 1 170 . 0 170 . 0 170 . 1 170 . 0 170 . 1 170 . 0 170 . 1 2 37 . 1 37 . 2 37 . 3 37 . 3 37 . 2 37 . 1 37 . 4 37 . 4 37 . 4 3 69 . 1 69 . 2 68 . 8 68 . 8 69 . 2 69 . 1 68 . 8 68 . 9 68 . 9 4 84 . 7 84 . 7 84 . 7 84 . 7 84 . 8 84 . 7 84 . 7 84 . 8 84 . 8 5 77 . 5 77 . 4 77 . 9 77 . 7 77 . 9 77 . 9 77 . 7 77 . 9 77 . 8 6 28 . 6 28 . 7 28 . 7 28 . 8 29 . 3 28 . 7 28 . 8 28 . 9 29 . 1 7 29 . 9 29 . 9 29 . 9 30 . 0 30 . 0 29 . 9 30 . 0 30 . 0 30 . 1 8 31 . 7 31 . 8 31 . 7 31 . 7 31 . 9 31 . 8 31 . 7 31 . 8 31 . 9 9 79 . 6 79 . 6 79 . 5 79 . 5 79 . 8 79 . 7 79 . 6 79 . 5 79 . 610 126 . 4 126 . 5 126 . 5 126 . 5 126 . 6 126 . 4 126 . 6 126 . 7 126 . 711 135 . 4 135 . 5 135 . 3 135 . 3 135 . 5 135 . 5 135 . 4 135 . 3 135 . 412 132 . 3 132 . 3 132 . 2 132 . 2 132 . 3 132 . 3 132 . 2 132 . 3 132 . 313 131 . 8 131 . 8 131 . 9 131 . 8 131 . 9 131 . 8 131 . 9 131 . 8 131 . 914 40 . 9 41 . 0 41 . 0 41 . 0 41 . 1 41 . 0 41 . 0 41 . 1 41 . 115 69 . 1 69 . 2 69 . 1 69 . 1 69 . 2 69 . 1 69 . 1 69 . 3 69 . 216 20 . 3 20 . 4 20 . 3 20 . 3 20 . 4 20 . 3 20 . 3 20 . 4 20 . 417 42 . 3 42 . 3 42 . 3 42 . 3 42 . 3 42 . 3 42 . 3 42 . 4 42 . 518 201 . 2 201 . 2 201 . 2 201 . 2 201 . 2 201 . 2 201 . 2 201 . 2 201 . 319 15 . 2 15 . 3 15 . 2 15 . 2 15 . 3 15 . 2 15 . 3 15 . 3 15 . 420 62 . 5 62 . 6 62 . 5 62 . 5 62 . 6 62 . 5 62 . 5 62 . 5 62 . 5 1 &# 39 ; 103 . 5 103 . 6 103 . 5 103 . 5 103 . 5 103 . 4 103 . 5 103 . 8 103 . 7 2 &# 39 ; 70 . 4 70 . 4 70 . 5 70 . 5 70 . 5 70 . 4 70 . 5 70 . 6 70 . 7 3 &# 39 ; 69 . 1 69 . 2 69 . 1 69 . 1 69 . 2 69 . 1 69 . 1 69 . 2 69 . 2 4 &# 39 ; 77 . 4 77 . 4 77 . 3 77 . 3 77 . 5 77 . 2 77 . 3 77 . 4 77 . 3 5 &# 39 ; 73 . 1 73 . 1 73 . 1 73 . 1 73 . 1 73 . 1 73 . 1 73 . 2 73 . 2 6 &# 39 ; 18 . 2 18 . 3 18 . 2 18 . 2 18 . 3 18 . 2 18 . 2 18 . 3 18 . 33 &# 39 ;- n ( ch . sub . 3 ). sub . 2 41 . 5 41 . 5 41 . 5 41 . 5 41 . 5 41 . 5 41 . 5 41 . 6 41 . 6 1 &# 34 ; 98 . 5 98 . 5 98 . 4 98 . 4 98 . 6 98 . 4 98 . 4 98 . 5 98 . 5 2 &# 34 ; 36 . 6 36 . 6 36 . 6 36 . 6 36 . 7 36 . 7 36 . 7 36 . 6 36 . 8 3 &# 34 ; 77 . 9 77 . 9 77 . 6 77 . 7 77 . 7 77 . 6 77 . 7 77 . 6 77 . 8 4 &# 34 ; 80 . 2 80 . 2 80 . 2 80 . 2 80 . 3 80 . 1 80 . 1 80 . 2 80 . 2 5 &# 34 ; 63 . 4 63 . 4 63 . 3 63 . 3 63 . 4 63 . 3 63 . 3 63 . 4 63 . 4 6 &# 34 ; 17 . 3 17 . 3 17 . 3 17 . 3 17 . 4 17 . 3 17 . 3 17 . 4 17 . 4 7 &# 34 ; 22 . 2 22 . 3 22 . 3 22 . 3 22 . 4 22 . 3 22 . 4 22 . 3 22 . 5 1 &# 34 ;&# 39 ; 100 . 1 100 . 2 100 . 0 99 . 9 100 . 2 100 . 1 100 . 0 100 . 1 100 . 1 2 &# 34 ;&# 39 ; 31 . 2 31 . 3 31 . 2 31 . 2 31 . 2 31 . 1 31 . 3 31 . 3 31 . 3 3 &# 34 ;&# 39 ; 18 . 4 18 . 5 18 . 5 18 . 5 18 . 7 18 . 6 18 . 5 18 . 6 18 . 5 4 &# 34 ;&# 39 ; 64 . 8 64 . 8 64 . 8 64 . 8 64 . 9 64 . 8 64 . 8 65 . 0 65 . 0 5 &# 34 ;&# 39 ; 73 . 7 73 . 8 73 . 7 73 . 7 73 . 7 73 . 6 73 . 7 73 . 9 73 . 9 6 &# 34 ;&# 39 ; 19 . 0 19 . 0 19 . 0 18 . 9 19 . 1 19 . 0 19 . 0 19 . 0 19 . 04 &# 34 ;&# 39 ;- n ( ch . sub . 3 ). sub . 2 40 . 7 40 . 7 40 . 6 40 . 6 40 . 6 40 . 6 40 . 7 40 . 7 40 . 8co . sub .-- ch . sub . 3 21 . 2 , 22 . 5 21 . 3 , 22 . 5 20 . 8 20 . 8 , 21 . 3 20 . 7 , 21 . 2 22 . 5 . sub .-- coch . sub . 3 170 . 4 , 170 . 8 170 . 4 , 170 . 8 170 . 5 170 . 6 , 170 . 8 170 . 5 , 170 . 8 170 . 4coch . sub . 2 . sub .-- ch . sub . 3 9 . 3 9 . 0 , 9 . 1 8 . 9 , 9 . 1 , 9 . 2 9 . 2 9 . 0 , 9 . 3 9 . 0 , 9 . 3 9 . 0 , 9 . 2co . sub .-- ch . sub . 2 ch . sub . 3 27 . 5 27 . 6 , 28 . 7 27 . 5 ( 2 ), 28 . 7 28 . 8 27 . 6 ( 2 ) 27 . 6 ( 2 ) 27 . 7 , 28 . 8 . sub .-- coch . sub . 2 ch . sub . 3 174 . 0 173 . 7 , 173 . 8 173 . 6 , 173 . 8 173 . 7 173 . 9 , 174 . 0 173 . 8 , 174 . 0 173 . 7 , 173 . 8 173 . 9coch . sub . 2 ch . sub . 2 . sub .-- ch . sub . 3 13 . 7 13 . 8 13 . 8 13 . 7coch . sub . 2 . sub .-- ch . sub . 2 ch . sub . 3 18 . 5 18 . 2 18 . 2 18 . 5co . sub .-- ch . sub . 2 ch . sub . 2 ch . sub . 3 36 . 1 37 . 3 37 . 4 36 . 2 . sub .-- coch . sub . 2 ch . sub . 2 ch . sub . 3 173 . 2 172 . 8 172 . 8 173 . 2__________________________________________________________________________ table 2__________________________________________________________________________fragments in mass spectrumm . sup .+ : ○ 1 ## str3 ## ## str4 ## compound no . fragment 1 2 3 4 5 6 7 8 9__________________________________________________________________________ ○ 1 + h983 996 + h997 -- 982 996 1024 + h997 1024 ○ 2 910 - h909 - h923 -- - h923 937 + h952 - 2h922 951 ○ 3 + h924 -- -- -- + h910 909 -- -- 937 ○ 4 + h 740 -- -- -- 740 -- -- 755 -- ○ 5 + h825 -- -- + h853 + h825 + h839 866 -- - h864 ○ 6 555 -- -- -- -- -- -- -- 564 ○ 7 416 430 416 + h431 416 430 444 + h417 444 ○ 8 - h 391 -- 405 -- 391 -- 405 -- 405 ○ 9 + h174 173 + h174 173 173 + h174 + h174 + h174 + h174 ○ 10 243 257 243 257 243 257 271 243 271 ○ 11 142 142 142 142 142 142 142 142 142__________________________________________________________________________ table 3______________________________________physico - chemical propertiescompoundno . tlc rf value [ α ]. sub . d . sup . 19 uv spectrum λ . sub . max . sup . meoh ( ε ) ______________________________________1 0 . 45 - 54 231 nm ( 24 , 400 ) 2 0 . 46 - 59 231 nm ( 22 , 000 ) 3 0 . 46 - 66 232 nm ( 29 , 500 ) 4 0 . 51 - 85 . 3 231 nm ( 22 , 500 ) 5 0 . 43 - 62 . 6 234 nm ( 35 , 300 ) 6 0 . 44 - 58 . 2 231 nm ( 24 , 500 ) 7 0 . 50 - 51 . 2 235 nm ( 22 , 500 ) 8 0 . 57 - 62 . 6 239 nm ( 38 , 600 ) 9 0 . 57 - 61 . 0 233 nm ( 37 , 700 ) ______________________________________ tlc : merck kiesel gel 60 [ developing solvent ( chloroform : methanol : concentrated ammonia = 10 : 1 : 0 . 01 ]- table 4______________________________________minimum inhibitory concentration ( mic , μ g / ml ) compound test bacteriano . a b c d e f g______________________________________1 3 . 12 & gt ; 100 1 . 56 3 . 12 & lt ; 0 . 1 & gt ; 100 & gt ; 1002 1 . 56 & gt ; 100 1 . 56 1 . 56 & lt ; 0 . 1 & gt ; 100 1003 3 . 12 & gt ; 100 3 . 12 3 . 12 & lt ; 0 . 1 & gt ; 100 & gt ; 1004 1 . 56 & gt ; 100 1 . 56 1 . 56 & lt ; 0 . 1 & gt ; 100 & gt ; 1005 3 . 12 & gt ; 100 3 . 12 1 . 56 & lt ; 0 . 1 & gt ; 100 & gt ; 1006 6 . 25 & gt ; 100 3 . 12 1 . 56 0 . 2 & gt ; 100 & gt ; 1007 3 . 12 & gt ; 100 1 . 56 1 . 56 & lt ; 0 . 1 & gt ; 100 & gt ; 1008 3 . 12 & gt ; 100 3 . 12 3 . 12 0 . 2 & gt ; 100 & gt ; 1009 3 . 12 & gt ; 100 3 . 12 3 . 12 0 . 2 & gt ; 100 & gt ; 100spmi 1 . 56 & gt ; 100 0 . 78 1 . 56 & lt ; 0 . 1 100 100______________________________________ remark : medium used : heart infusion agar ( ph 7 . 0 ) a : staphylococcus aureus kb210 b : staphylococcus aureus kb199 c : bacillus subtilis kb211 d : bacillus cereus kb143 e : micrococcus ruteus kb212 f : escherichia coli kb213 g : klebsiella pneumoniae kb214 spmi : spiramycin i a test example of the therapeutic effect of compound ( i ) in experimental test mice protection test will be given below : animal : ddy - strain male mice , 19 ± lg , one group consisting of ten mice . test bacterium : streptococcus pneumoniae type iii , inoculation rate : 1 . 1 × 10 2 cfu / mouse . the predetermined amount of the precultured test bacteria was inoculated via i . p . medicament administration : 60 mg / kg of each of the medicaments was suspended in 0 . 3 % cmc , and orally administered just after the inoculation of bacteria . the number of survived animals on the 7th day is shown in table 5 . table 5__________________________________________________________________________infection - healing effectcompound no . 1 2 3 4 5 6 7 8 9 acspm spmi__________________________________________________________________________number of survived 5 5 5 10 3 10 5 10 8 6 0mice out of 10 mice__________________________________________________________________________ acspm : acetylspiramycin compound ( i ) can be produced by the three steps shown in table 6 , that is , by each of ( 1 ) acylation ( r 1 ) of hydroxyl groups at the 2 &# 39 ;- and 4 &# 34 ;- positions or hydroxyl groups at the 3 -, 2 &# 39 ;-, and 4 &# 34 ;- positions , [ production of compound ( ii )], ( 2 ) acylation ( r 2 ) of the hydroxyl group at the 4 &# 34 ;- position or hydroxyl groups at the 4 &# 34 ;- and 3 - positions together with a migration reaction of the acyl group ( r 1 ) at the 4 &# 34 ;- position to hydroxyl group at 3 &# 34 ;- position [ production of compound ( iii )], and ( 3 ) removal of the acyl group at the 2 &# 39 ;- position [ production of compound ( i )]. spiramycin i has , in total , 4 hydroxyl groups at the 2 &# 39 ;-, 3 &# 34 ;-, 4 &# 34 ;- and 3 - positions in the structure . the reactivity of these hydroxyl groups to acylation under basic conditions is generally in the order of 2 &# 39 ;& gt ; 4 &# 34 ;& gt ; 3 & gt ; 3 &# 34 ;. the desired acyl groups can be introduced on the hydroxyl groups at the 2 &# 39 ;- and 4 &# 34 ;- positions or on the hydroxyl groups at the 3 -, 2 &# 39 ;- and 4 &# 34 ;- positions by setting appropriate reaction conditions in view of the differences in reactivity . the hydroxyl group at the 3 &# 34 ;- position of spiramycin i is considerably low in reactivity and is hardly susceptible to direct acylation according to the ordinary acylation procedure . an acyl group can be introduced at the 3 &# 34 ;- position while migrating the acyl group introduced beforehand at the 4 &# 34 ;- position to the 3 &# 34 ;- position and introducing a new acyl group at the 4 &# 34 ;- position . such migration reaction of the acyl group is well known in sugar chemistry . when a spiramycin i derivative , whose hydroxyl group at the 3 - position or 2 &# 39 ;- position is free , is used as the starting compound , acyl groups can also be introduced at these positions at the same time . in this manner , a tetraacyl derivative of spiramycin i represented by formula ( iii ), which has the same or different acyl groups at the 3 -, 2 &# 39 ;-, 3 &# 34 ;- and 4 &# 34 ;- positions , can be obtained . compound ( iii ) can also be produced by acylating 3 &# 34 ;, 4 &# 34 ;- di - o - acylspiramycin i disclosed in japanese published unexamined patent application no . 136589 / 1983 in a similar manner . then , the present compound , 3 , 3 &# 34 ;, 4 &# 34 ;- tri - o - acylspiramycin i can be produced by selective removal of the acyl group at the 2 &# 39 ;- position . table 6______________________________________production procedure______________________________________ ## str5 ## ## str6 ## ## str7 ## ( ii ) ## str8 ## ## str9 ## ( iii ) ## str10 ## ## str11 ## ( i ) ______________________________________ ( wherein r . sub . 1 , r . sub . 2 and r . sub . 3 have the same meanings as defined above , and ## str12 ## 2 &# 39 ;, 4 &# 34 ;- di - o - acylspiramycin i or 3 , 2 &# 39 ;, 4 &# 34 ;- tri - o - acylspiramycin i represented by compound ( ii ) can be produced by the reaction of spiramycin i with an acyl halide or acid anhydride of a carboxylic acid represented by the formula c m h 2m + 1 co 2 h ( wherein m has the same meaning as defined above ) or with an acyl halide or acid anhydride of a carboxylic acid represented by the formula c n h 2n + 1 co 2 h ( wherein n has the same meaning as defined above ) in the presence of a base in an organic solvent having no active hydrogen atom at 10 °- 120 ° c . for 6 hours to 20 days . the organic solvent having no active hydrogen atom is exemplified by chloroform , dichloromethane , dichloroethane , trichloroethane , benzene , toluene , acetone , ethyl acetate , dioxane , tetrahydrofuran , dimethylformamide , etc ., preferably , chloroform or dichloromethane . the base is exemplified by pyridine , quinoline , triethylamine , sodium carbonate , potassium carbonate , preferably , pyridine . when pyridine is used as a base , it is possible to dispense with the organic solvent having no active hydrogen . the acyl halide and carboxylic acid anhydride are exemplified by the acid chlorides , acid bromides and acid anhydrides of acetic acid , propionic acid , butyric acid , valeric acid and isovaleric acid . when the reaction is carried out with 5 to 20 molar equivalent of an acid anhydride or acyl halide in pyridine - chloroform or - dichloromethane , or in pyridine , at 10 °- 30 ° c . for 6 hours to 12 days , 2 &# 39 ;, 4 &# 34 ;- di - o - acyl compound is predominantly obtained . when the reaction is carried out with 15 to 40 molar equivalent of an acid anhydride or acyl halide in pyridine at 30 °- 120 ° c . for 5 - 20 days , 3 , 2 &# 39 ;, 4 &# 34 ;- tri - o - acyl compound is predominantly obtained . in either case , the products can be separated and purified by silica gel column chromatography . compound ( iii ) can be produced by reacting compound ( ii ) with an acyl halide or an acid anhydride of a carboxylic acid represented by the formula c n h 2n + 1 co 2 h ( wherein n has the same meaning as defined above ) when using an acyl halide or an acid anhydride of a carboxylic acid represented by the formula c m h 2m + 1 co 2 h in the above step ( 1 ), or with an acyl halide or an acid anhydride of a carboxylic acid represented by the formula c m h 2m + 1 co 2 h ( wherein m has the same meaning as defined above ) when using an acyl halide or an acid anhydride of a carboxylic acid represented by the formula c n h 2n + 1 co 2 h in the above step ( 1 ) in the presence of a base in an organic solvent having no active hydrogen atom at 25 °- 150 ° c . for 24 hours to 6 days . the organic solvent having no active hydrogen atom is exemplified by chloroform , dichloromethane , dichloroethane , trichloroethane , benzene , toluene , acetone , ethyl acetate , dioxane , tetrahydrofuran , dimethylformamide , etc ., preferably chloroform . the base is exemplified by pyridine , dimethylaminopyridine , quinoline , triethylamine , tribenzylamine , sodium carbonate , potassium carbonate , sodium hydroxide , potassium hydroxide , etc ., preferably , pyridine , dimethylaminopyridine and triethylamine , or their combinations . when pyridine is used as the base , it is possible to dispense with the organic solvent having no active hydrogen . the acyl halide and carboxylic acid anhydride are exemplified by acid chlorides , acid bromides and acid anhydrides of acetic acid , propionic acid , butyric acid , valeric acid , and isovaleric acid . it is the most preferable condition to subject compound ( ii ) to reflux with 20 to 40 molar equivalent of an acid anhydride in chloroform containing triethylamine and dimethylaminopyridine with heating for 40 - 100 hours , or to subject compound ( ii ) to reaction with 20 to 40 molar equivalent of an acid anhydride in pyridine containing dimethylaminopyridine at 70 °- 115 ° c . for 40 - 150 hours . in either case , the yield is considerably lowered , when dimethylaminopyridine is dispensed with . this step is also applicable to the production of 3 &# 34 ;, 4 &# 34 ;- di - o - acylspiramycin i disclosed in japanese patent application no . 136589 / 1983 and the journal of antibiotics vol . 37 , no . 7 , pp . 760 - 772 ( 1984 ), where an acyl group can be selected for r 3 , irrespective of the species of r 1 and r 2 in compound ( iii ). the reaction product can be introduced into the successive step for removing the protective group without any purification , but it can be purified , if required , by silica gel column chromatography . compound ( i ) can be produced by removing the 2 &# 39 ;- o - acyl group ( partly 18 - enolacyl group ) of compound ( iii ). that is , compound ( i ) can be obtained by dissolving compound ( iii ) in an alcohol or water - containing organic solvent , and subjecting the solution to reaction at 20 °- 100 ° c . methanol , ethanol , etc . can be used as the alcohol , and mixtures of methanol , ethanol , acetone , 2 - methoxyethanol , ethylene glycol , ethylene glycol dimethyl ether , tetrahydrofuran , dioxane , dimethylformamide , etc . with water can be used as the water - containing organic solvent . particularly , water - containing methanol is preferable . the reaction product is purified by silica gel column chromatography to obtain pure compound ( i ). first , 5 . 05 g of spiramycin i as vacuum - dried at 60 ° c . for 3 hours is dissolved in 60 ml of pyridine , and 6 ml of acetic anhydride is added thereto with ice cooling and stirring . then , the temperature of the mixture is brought back to room temperature and the mixture is allowed to stand . after 6 days , 3 ml of acetic anhydride is further added thereto , and the mixture is heated at 50 ° c . for 5 days . then , 10 ml of methanol is added to the reaction mixture , and the mixture is allowed to stand for one hour . then , the mixture is diluted with 500 ml of chloroform , and washed with 500 ml of water . the chloroform solution is dried over sodium sulfate , and then concentrated under reduced pressure . the thus obtained brown glass - like substance is purified by silica gel column chromatography using benzene : acetone = 5 : 2 as a developing solvent system , whereby 636 mg of the captioned compound can be obtained in a colorless , glass - like state ( 11 . 0 %). tlc rf value : 0 . 88 ( chloroform : methanol : concentrated ammonia water = 10 : 1 : 0 . 01 ), [ α ] d 19 - 93 . 6 ° ( c 1 , chloroform ). first , 920 mg of 3 , 2 &# 39 ;, 4 &# 34 ;- tri - o - acetylspiramycin i and 98 mg of n , n - dimethylaminopyridine are dissolved in 25 ml of chloroform , and then 3 . 2 ml of triethylamine and subsequently , 3 . 2 ml of propionic anhydride are added thereto . the mixture is gently heated and refluxed . after 98 hours , the temperature of the reaction mixture is brought back to room temperature , and then 5 ml of methanol is added thereto . the mixture is allowed to stand at room temperature for one hour . the reaction mixture is diluted with 90 ml of ethyl acetate and washed with 90 ml of an aqueous saturated sodium hydrogen carbonate solution and then with 90 ml of water . the ethyl acetate solution is dried over sodium sulfate and concentrated under reduced pressure , whereby a brown solid is obtained . the solid is dissolved in 45 ml of 70 % aqueous methanol and the solution is heated at 50 ° c . for 62 hours . the reaction solution is diluted with 70 ml of chloroform , and washed with 90 ml of water , and concentrated under reduced pressure , whereby a brown , glass - like substance is obtained . the thus obtained , glass - like substance is purified by silica gel column chromatography , using benzene : acetone = 4 : 1 as a developing solvent system , whereby 387 mg . of the captioned compound can be obtained as a colorless powder ( 41 . 4 %). the physico - chemical properties of the substance are shown in tables 1 - 3 . first , 1 . 00 g of 3 , 2 &# 34 ;, 4 &# 34 ;- tri - o - acetylspiramycin i produced according to example ( 1 - 1 ) and 107 mg of n , n - dimethylaminopyridine are dissolved in 27 ml of chloroform , and then 3 . 4 ml of triethylamine and subsequently 4 . 3 ml of butyric anhydride are added thereto . the mixture is gently heated and refluxed . after 89 hours , the temperature of the reaction solution is brought back to room temperature , and then 5 ml of methanol is added thereto . the mixture is allowed to stand at room temperature for one hour . the reaction solution is diluted with 100 ml of ethyl acetate , and washed with 100 ml of an aqueous saturated sodium hydrogen carbonate solution and then with 100 ml of water . the ethyl acetate solution is dried over sodium sulfate , and concentrated under reduced pressure , whereby a brown solid is obtained . the solid is dissolved in 49 ml of 70 % aqueous methanol and the solution is heated at 50 ° c . for 86 hours . the reaction solution is diluted with 70 ml of chloroform , washed with 90 ml of water , and concentrated under reduced pressure , whereby a brown , glass - like substance is obtained . the thus obtained , glass - like substance is purified by silica gel chromatography , using benzene : acetone = 4 : 1 as a developing solvent system , whereby 327 mg of the captioned compound is obtained as a colorless power ( 31 . 8 %). the physico - chemical properties of the compound are shown in tables 1 - 3 . first , 5 . 05 g of spiramycin i as vacuum - dried at 60 ° c . for 3 hours is dissolved in 76 ml of pyridine , and 12 ml of propionic anhydride is added thereto with ice ccoling and stirring . the reaction mixture is heated at 50 ° c . for 7 days , and then 7 . 5 ml of water is added thereto . the mixture is allowed to stand at room temperature for one hour . the reaction mixture is diluted with 500 ml of chloroform and washed with 500 ml of water . the chloroform solution is dried over sodium sulfate , and concentrated under reduced pressure . the thus obtained , brown , glass - like substance is purified by silica gel column chromatography , using benzene : acetone = 3 : 1 as a developing solvent system , whereby 1 . 40 g of the captioned substance is obtained in a colorless glass - like state ( 23 . 1 %). tlc ( kiesel gel 60 is used , and will hereinafter be used ). rf value : 0 . 89 ( chloroform : methanol : concentrated ammonia water = 10 : 1 : 0 . 01 ), [ α ] d 19 - 85 . 8 ° c . ( c 1 , chloroform ) first , 940 mg of 3 , 2 &# 39 ;, 4 &# 34 ;- tri - o - propionylspiramycin i and 95 mg of n , n - dimethylaminopyridine are dissolved in 26 ml of chloroform , and then 3 . 3 ml of triethylamine and subsequently , 2 . 5 ml of acetic anhydride are added thereto . then , the mixture is gently heated and refluxed . after 164 hours , the temperature of the reaction solution is brought back to room temperature and 5 ml of methanol is added thereto . the mixture is allowed to stand at room temperature for one hour . then , the reaction mixture is diluted with 100 ml of ethyl acetate , and washed with 100 ml of an aqueous saturated sodium hydrogen carbonate solution and then with 100 ml of water . then , the ethyl acetate solution is dried over sodium sulfate and concentrated under reduced pressure , whereby a brown solid is obtained . the solid is dissolved in 49 ml of 70 % aqueous methanol and the solution is heated at 50 ° c . for 56 hours . the reaction solution is diluted with 70 ml of chloroform , washed with 90 ml of water and concentrated under reduced pressure , whereby a brown , glass - like substance is obtained . the thus obtained glass - like substance is purified by silica gel column chromatography , using benzene : acetone = 5 : 1as a developing solvent system , whereby 140 mg of the captioned compound is obtained as a colorless powder ( 15 . 1 %). the physico - chemical properties of the substance are shown in tables 1 - 3 . first , 5 . 05 g of spiramycin i as vacuum - dried at 60 ° c . for 3 hours is dissolved in 60 ml of chloroform , and then 9 . 5 ml of pyridine and subsequently , 7 . 8 ml of propionic anhydride are added thereto with ice cooling and stirring . then , the temperature of the mixture is brought back to room temperature and the mixture is allowed to stand . after 4 days , 4 . 8 ml of pyridine and 3 . 9 ml of propionic anhydride are further added thereto , and the mixture is allowed to stand at room temperature . after 6 days , 20 ml of methanol is added to the reaction mixture , and the mixture is allowed to stand for one hour , diluted with 400 ml of chloroform , and washed with 450 ml of water . the chloroform solution is dried over sodium sulfate and concentrated under reduced pressure . the thus obtained brown , glass - like substance is purified by silica gel column chromatography , using benzene : acetone = 5 : 2 as a developing solvent system , whereby 3 . 13 g of the captioned substance can be obtained in a colorless glass - like state ( 54 . 7 %). tlc rf value : 0 . 49 ( chloroform : methanol : concentrated ammonia water = 10 : 1 : 0 . 01 ), [ α ] d 19 - 88 ° ( c 1 , chloroform ). first , 1 . 00 g of 2 &# 39 ;, 4 &# 34 ;- di - o - propionylspiramycin i and 103 mg of n , n - dimethylaminopyridine are dissolved in 27 ml of chloroform , and then 3 . 5 ml of triethylamine and subsequently , 3 . 4 ml of propionic anhydride are added thereto . the mixture is gently heated and refluxed . after 84 hours , the temperature of the reaction solution is brought back to room temperature , and then 5 ml of methanol is added thereto . the mixture is allowed to stand at room temperature for one hour . the reaction mixture is diluted with 100 ml of ethyl acetate , and washed with 100 ml of an aqueous saturated sodium hydrogen carbonate solution and then with 100 ml of water . the ethyl acetate solution is dried over sodium sulfate and concentrated under reduced pressure , whereby a brown solid is obtained . the solid is dissolved in 49 ml of 70 % aqueous methanol and the solution is heated at 50 ° c . for 120 hours . the reaction solution is diluted with 70 ml of chloroform , washed with 90 ml of water and concentrated under reduced pressure , whereby a brown , glass - like substance is obtained . the thus obtained glass - like substance is purified by silica gel column chromatography , using benzene : acetone = 4 : 1 as a developing solvent system , whereby 300 mg of the captioned compound is obtained as a colorless powder ( 28 . 3 %). the physico - chemical properties of the substance are shown in tables 1 - 3 . first , 1 . 00 g of 2 &# 39 ;, 4 &# 34 ;- di - o - propionylspiramycin i produced according to example ( 4 - 1 ) and 103 mg of n , n - dimethylaminopyridine are dissolved in 27 ml of chloroform , and 3 . 5 ml of triethylamine and then 2 . 7 ml of acetic anhydride are added thereto . the mixture is gently heated and refluxed . after 60 hours , the temperature of the reaction solution is brought back to room temperature , and then 5 ml of methanol is added thereto the mixture is allowed to stand at room temperature for one hour . then , the reaction mixture is diluted with 100 ml of ethyl acetate and washed with 100 ml of a saturated aqueous sodium hydrogen carbonate solution and then with 100 ml of water . the ethyl acetate solution is dried over sodium sulfate and concentrated under reduced pressure , whereby a brown solid is obtained . the solid is dissolved in 49 ml of 70 % aqueous methanol , and the solution is heated at 50 ° c . for 98 hours . the reaction solution is diluted with 70 ml of chloroform , washed with 90 ml of water , and concentrated under reduced pressure , whereby a brown , glass - like substance is obtained . the thus obtained glass - like substance is purified by silica gel column chromatography , using benzene : acetone = 5 : 1 as a developing solvent system , whereby 370 mg of the captioned compound is obtained as a colorless powder ( 35 . 8 %). the physico - chemical properties of the substance are shown in tables 1 - 3 . first , 5 . 05 g of spiramycin i as vacuum - dried at 60 ° c . for 3 hours is dissolved in 60 ml of chloroform , and 9 . 5 ml of pyridine and then 9 . 5 ml of butyric anhydride are added thereto with ice cooling and stirring . the temperature of the mixture is brought back to room temperature and then , the mixture is allowed to stand . after 2 days , 4 . 8 ml of pyridine and 4 . 8 ml of butyric anhydride are added thereto . after 8 days 4 . 8 ml of pyridine and 4 . 8 ml of butyric anhydride are further added thereto , and the mixture is allowed to stand at room temperature . after 9 days , 20 ml of methanol is added to the reaction mixture and the mixture is allowed to stand for one hour . then , the mixture is diluted with 400 ml of chloroform and washed with 450 ml of water . the chloroform solution is dried over sodium sulfate , and concentrated under reduced pressure , and the thus obtained , brown , glass - like substance is purified by silica gel column chromatography , using benzene : acetone = 5 : 2 as a developing solvent system , whereby 3 . 26g of the captioned substance is obtained in a colorless glass - like state ( 55 . 3 %). tlc rf value : 0 . 46 ( chloroform : methanol : concentrated ammonia water = 10 : 1 : 0 . 01 ), [ α ]. sub . d 19 - 91 ° ( c 1 , chloroform ). first , 1 . 00 g of 2 &# 39 ;, 4 &# 34 ;- di - o - butyrylspiramycin i and 108 mg of n , n - dimethylaminopyridine are dissolved in 27 ml of chloroform , and then 3 . 5 ml of triethylamine and subsequently 2 . 8 ml of acetic anhydride are added thereto . then , the mixture is gently heated and refluxed . after 75 hours , the temperature of the reaction solution is brought back to room temperature , and then 5 ml of methanol is added thereto . the mixture is allowed to stand at room temperature for one hour . the reaction solution is diluted with 100 ml of ethyl acetate and washed with 100 ml of a saturated aqueous sodium hydrogen carbonate solution and then with 100 ml of water . the ethyl acetate solution is dried over sodium sulfate and concentrated under reduced pressure , whereby a brown solid is obtained . the solid is dissolved in 49 ml of 70 % aqueous methanol , and the solution is heated at 50 ° c . for 67 hours . the reaction solution is diluted with 70 ml of chloroform , washed with 90 ml of water and concentrated under reduced pressure , whereby a brown , glass - like substance is obtained . the thus obtained glass - like substance is purified by silica gel column chromatography , using benzene : acetone = 3 : 1 as a developing solvent system , whereby 450 mg of the captioned compound is obtained as a colorless powder ( 44 . 3 %). the physico - chemical properties of the substance are shown in tables 1 - 3 . first , 1 . 00 g of 2 &# 39 ;, 4 &# 34 ;- di - o - butyrylspiramycin i produced according to example ( 6 - 1 ) and 108 mg of n , n - dimethylaminopyridine are dissolved in 49 ml of chloroform and then 3 . 5 ml of triethylamine and subsequently 3 . 6 ml of propionic anhydride are added thereto . the mixture is gently heated and refluxed . after 97 hours , the temperature of the reaction solution is brought back to room temperature , and 5 ml of methanol is added thereto . the mixture is allowed to stand at room temperature for one hour . the reaction mixture is diluted with 100 ml of ethyl acetate , and washed with 100 ml of a saturated aqueous sodium hydrogen carbonate solution and then with 100 ml of water . the ethyl acetate solution is dried over sodium sulfate , and concentrated under reduced pressure , whereby a brown solid is obtained . the solid is dissolved in 49 ml of 70 % aqueous methanol and the solution is heated at 50 ° c . for 44 hours . the reaction solution is diluted with 70 ml of chloroform , washed with 90 ml of water , and concentrated under reduced pressure , whereby a brown , glass - like substance is obtained . the thus obtained , glass - like substance is purified by silica gel column chromatography , using benzene : acetone = 5 : 1 as a developing solvent system , whereby 308 mg of the captioned compound is obtained as a colorless powder ( 29 . 5 %). the physico - chemical properties of the substance are shown in tables 1 - 3 . first , 2 . 00 g of spiramycin i as vacuum - dried at 60 ° c . for 3 hours is dissolved in 30 ml of pyridine , and 2 . 4 ml of acetic anhydride is added thereto with ice cooling and stirring . the temperature of the reaction mixture is brought back to room temperature and then , the mixture is allowed to stand for 20 hours at room temperature . then , 8 ml of methanol is added thereto and the mixture is allowed to stand for one hour . then , the mixture is diluted with 100 ml of chloroform and washed with 100 ml of a saturated aqueous sodium hydrogen carbonate solution and then with 100 ml of water . the chloroform solution is dried over sodium sulfate , and concentrated under reduced pressure . the resulting brown powder is purified by silica gel column chromatography , using benzene : acetone = 3 : 1 - 2 : 1 ( the ratio being gradually changed ) as a developing solvent system , whereby 1 . 02 g of the captioned compound is obtained as a colorless powder ( 46 . 3 %). tlc rf value : 0 . 51 ( chloroform : methanol : concentrated ammonia water = 10 : 1 : 0 . 01 ), [ α ] d 19 - 88 . 1 ° ( c 1 . 0 , chloroform ). first , 1 . 02g of 2 &# 39 ;, 4 &# 34 ;- di - o - acetylspiramycin i and 113 mg of n , n - dimethylaminopyridine are dissolved in 27 ml of chloroform , and 3 . 5 ml of triethylamine and then 3 . 7 ml of propionic anhydride are added thereto . the mixture is gently heated under reflux . after 74 hours , the temperature of the reaction solution is brought back to room temperature , 5 ml of methanol is added thereto and the mixture is allowed to stand for one hour . the reaction mixture is diluted with 100 ml of ethyl acetate , and washed with 100 ml of a saturated aqueous sodium hydrogen carbonate solution and then with 100 ml of water . the ethyl acetate solution is dried over sodium sulfate and concentrated under reduced pressure to obtain a brown powder . the powder is dissolved in 49 ml of 70 % aqueous methanol and the solution is heated at 50 ° c . for 96 hours . the reaction mixture is diluted with 70 ml of chloroform , washed with 70 ml of water and concentrated under reduced pressure to obtain a brown glass - like substance . the glass - like substance is purified by silica gel column chromatography , using chloroform : methanol : concentrated ammonia water = 50 : 1 : 0 . 01 as a developing solvent system , whereby 419 mg of the captioned compound is obtained as a colorless powder ( 38 . 9 %). the physico - chemical properties of the substance are shown in tables 1 - 3 . first , 693 mg of 3 , 2 &# 39 ;, 4 &# 34 ;- tri - o - propionylspiramycin i produced according to example ( 3 - 1 ) and 74 mg of n , n - dimethylaminopyridine are dissolved in 19 ml of chloroform , 2 . 4 ml of triethylamine and then 3 . 0 ml of acetic anhydride are added thereto , and the mixture is gently heated under reflux . after 48 hours , the temperature of the reaction mixture is brought back to room temperature , 5 ml of methanol is added thereto and the mixture is allowed to stand for one hour . the reaction mixture is diluted with 70 ml of ethyl acetate and washed with 70 ml of a saturated aqueous sodium hydrogen carbonate solution and then with 70 ml of water . the ethyl acetate solution is dried over sodium sulfate and concentrated under reduced pressure to obtain a brown solid . the solid is dissolved in 34 ml of 70 % aqueous methanol and the solution is heated at 50 ° c . for 50 hours . the temperature of the reaction solution is brought back to room temperature and the solution is diluted with 70 ml of chloroform and washed with 70 ml of water . the chloroform solution is dried over sodium sulfate and concentrated under reduced pressure to obtain a brown glass - like substance . the substance is purified by silica gel chromatography , using chloroform : methanol : concentrated ammonia water = 50 : 1 : 0 . 01 as a developing solvent system , whereby 221 mg of the captioned compound is obtained as a colorless powder ( 31 . 5 %). the physico - chemical properties of the substance are shown in tables 1 - 3 . comparison of healing effects of acetylspiramycin and 3 , 3 &# 34 ;, 4 &# 34 ;- tri - o - acetylspiramycin i on test infection in mice animal : ddy - strain male mice , 19 ± lg , one group consisting of ten mice . test bacterium : streptococcus pneumoniae type iii . inoculation rate : 1 . 1 × 10 2 cfu / mouse . the predetermined amount of the precultured test bacteria was inoculated via i . p .. medicament administration : each of the medicaments was suspended in 0 . 3 % cmc , and orally administered just after the inoculation of bacteria . ed 50 values of acetylspiramycin and 3 , 3 &# 34 ;, 4 &# 34 ;- tri - o - acetylspiramycin calculated according to the probit method based on number of survived animals on the 7th day were almost the same , being 71 . 1 mg / kg and 69 . 5 mg / kg respectively .