text: although the disclosure herein refers to certain described embodiments , it is to be understood that embodiments presented herein are by way of example and not by way of limitation . the intent of this disclosure , while discussing exemplary embodiments , is that the following detailed description be construed to cover all modifications , alternatives , and equivalents of the embodiments as may fall within the spirit and scope of the invention as defined by the appended claims . it is to be understood and appreciated that the process steps and formulations described herein do not cover a complete process flow for operations involving compounding , tissue augmentation , and repair . the present invention may be practiced in conjunction with various techniques that are conventionally used in the art , and only so much of the commonly practiced process steps are included herein as are necessary to provide an understanding of the present invention . the methods and systems described herein can allow physicians to repair , treat or augment a patient &# 39 ; s tissue or fluids using an enhanced tissue treatment implant which may take the form of a biocompatible augmentation or repair material . the tissue treatment material can comprise microparticles and a biocompatible carrier medium in the form of a mixture of cross linked native bovine or other mammalian collagen blended with partially denatured gelatinous bovine or other mammalian collagen or biocompatible gelatin , with the ratios of those two components being controlled . in one aspect of the present invention , the denatured gelatinous bovine or other mammalian , avian or reptilian collagen provides the ability to hold the microparticles uniformly in suspension after blending . in another aspect of the present invention , the microparticles are substantially uniformly suspended within the biocompatible carrier at ambient temperature , thus making storage of the product less costly and more environmentally and functionally suitable . in one embodiment , the crosslinked native fibrillar mammalian , avian or reptilian collagen component comprises a melting point greater than 37 . 0 ° c . in one preferred embodiment the t m will be in the range of about 37 . 0 ° c . to about 65 . 0 ° c . in another embodiment , the crosslinked native fibrillar mammalian , avian or reptilian collagen component provides increased resistance to deformation and an increased shear resistance or shear modulus . for example , the collagen component can have a shear modulus in the range of about 10 , 000 to 200 , 000 pascals under the typical conditions of use , and density in the range of about 1 . 04 to 1 . 20 g / ml . in another embodiment , the crosslinked native fibrillar mammalian , avian or reptilian collagen component comprises a resorption rate of about 4 months to about 8 months in situ . in certain implementations , the enhanced carrier material may comprise cross linked native bovine collagen , blended with the microparticles and a gelatinizing agent derived from animal , vegetable , or synthetic sources . as for composition , the microspheres in accordance with certain implementations of the present invention can comprise a cured polymer , such as a polymethacrylate ( pma ), a polymethylmethacrylate ( pmma ), a calcium hydroxyapetite ( caha ), polypropylene ( pp ), polytetrafluoroethylene ( ptfe ), hydroxyapetite ( hap ), polylactic acid ( pla ) beta tricalcium phosphate , calcium phosphate tribasic , hyaluronic acid ( ha ), polyester , polyvinyl alcohol ( pva ), poly lactic co glycolic acid ( plga ), polyetheretherketone ( peek ), polyethylene oxides ( peo ), albumin , phopholipids , polyethylene glycols ( peg ), calcium alginates , fibrin , polysaccharides , polyoxyesters , polyoxaamides , polyamides , trisacryl gelatin , and copolymers thereof , or any other solid or semisolid biocompatible material . in one implementation , the microparticles can comprise solid microparticles , which may take form in one embodiment of non porous beads . in other implementations , the microparticles may not be altogether solid , such as implementations involving hollow and / or porous microparticles . said hollow and / or porous microparticles may be used to deliver enhancements to the carrier , including but not limited to , antimicrobial agents , antibiotic agents , anti inflammatory agents , growth factors , or adhesion prevention agents in a time release mechanism . sacrificial coatings surrounding the hollow and porous microspheres could erode away over time , allowing the release of said agents that were preloaded into the microsphere cores before they were coated . the microparticles can have a short - term effectiveness of up to about 6 months , a medium term effectiveness of up to about 3 years , or a long term effectiveness of significantly more than 3 years . the microparticles can be made at least in part of biocompatible materials , such as , for example , but not limited to , one or more of any type of collagen , hyaluronic acid ( e . g ., animal derived , human derived and / or tissue / cell culture derived ), genetically altered cells , tissues , organisms , genetically altered or not ( e . g ., purified cytoskeleton of unicellular and / or multi - cellular algae and / or other organisms ), whether cross - linked or not cross - linked , or made of a synthetic and / or polymeric material , such as , for example , polylactic acid , organic compounds , inorganic compounds , ceramic materials , calcium alginates , albumin , beta tricalcium phosphates , phospholipids , polysaccharides , polyoxaesters , polyoxaamide , polyamines , polyethylene glycols ( peg ), polyethylene oxides ( peos ), polymethacrylate ( pma ), polymethylmethacrylate ( pmma ), polypropylene , polytetrafluoroethylene ( ptfe ), and combinations thereof . as used herein , the term “ microparticles ” refers to microparticles ( e . g ., in a dust or powder form ) possessing a mean diameter of at least about 10 microns . referring to fig2 , a microsphere in one embodiment of the present invention can have a smooth surface and a diameter “ d ”. typically , the average diameter will be greater than about 15 microns rendering the microparticles too large to be consumed by monocytes . the microparticles can have diameters sufficient to keep them from being washed away through lymph tracts or other tissue tracts from the implantation site . if the microparticles do not have a spherical form , then the diameter as used herein may refer to the greatest diameter of the smallest cross sectional area . it is , however , also possible to use different sized ( e . g ., smaller ) microparticles . typically , the microparticles will have an average diameter less than about 200 microns . in representative embodiments , the microparticles can have an average diameter of about 15 to about 200 microns and in certain implementations from about 15 to about 60 microns . it may be possible in modified embodiments for diameters to range from about 10 microns to about 500 microns . in certain configurations , the microparticles are small enough to be injected from a syringe through a fine gauge cannula or an injection needle to the desired treatment region . microparticles having the diameters specified herein may have a relatively minimal effect on surrounding tissues . the microparticles used according to exemplary embodiments of the present invention have smooth or non - turbulent surfaces that are , for example , free from one or more of corners and edges . in similar implementations , the microparticles of these embodiments may be formed at least in part not to have sharp transitions on their surfaces , wherein transitions are for instance found at such corners and edges . in addition , they may be formed not to have peaks of any kind or tapered projections . consequently , transitions from one outer surface to the other outer surface of one or more of the microparticles as used according to these embodiments of present invention can occur in a continuous manner . if such transitions are present , as is the case for the edges of a cube , such transitions can be smoothed or rounded . due to the smooth surfaces of the above - described types of microparticles , damage or irritation to cells and other tissue structures can be altered , minimized , or avoided . in addition , according to an aspect of the present invention , the danger of causing reactions of the tissue , such as foreign body reactions or granulous formation in response to sharp edges , which may be followed by infections , can be altered , attenuated , or eliminated . in addition to spherical forms , the microparticles may comprise , as a few examples , one or more of elliptical ( e . g ., extruded , molded and / or machined ) or cylindrical ( e . g ., extruded , molded and / or machined ) forms , and further may comprise , for example , one or more of smoothed or rounded corners , edges , peaks , and projections . other implementations may not include smoothed or rounded corners , edges , peaks , and projections . according to exemplary embodiments of the present invention , microparticles which are crystalline ( for instance needle - shaped ) or microparticles which have been obtained by mechanically breaking up greater units into smaller pieces , may not be used , in some embodiments , to the extent the microparticles possess the above - mentioned sharp edges and corners . in modified embodiments , these types of microparticles may be used in whole or in part , such as , for example , embodiments comprising microparticles possessing one or more of sizes between about 10 microns and about 500 microns ; an absence of smooth surfaces ; an absence of corners , edges , peaks , or projections ; and an absence of smooth or rounded corners , edges , peaks , and projections . the microparticles may comprise one or more of a solid body , a porous surface ( e . g ., a surface impregnated with a material that is subsequently dissolved out ), a porous body ( e . g ., formed by coating an expandable material and then heating , so that the expandable material inside forms a gas and expands forming channels to the surface ), a hollow interior ( e . g ., formed by providing a feed tube into a center of a die for forming a microparticle , whereby as material is passed into the die for molding gas is injected into a central area of the material within the die ), a shell - like structure ( e . g ., formed by coating and / or dissolving - out an interior beneath the coating ), a metallic coating , a carbon coating , a carbon nanotube coating , a non - coated surface , components thereof , and combinations thereof in any ratio . according to certain implementations , surfaces of the microparticles of the present invention do not have pores . in other implementations of the present invention , however , parts or all of the surfaces of the microparticles may comprise pores . in certain implementations , dynamically balanced microparticles and in particular instances microparticles having elliptic or spherical forms can be used . in some examples , it is possible to use microparticles of a different geometrical form , and , when in the context of smooth - surfaced implementations , all or a majority of the microparticles may have smooth or smoothed - off surfaces . when formed with smooth surfaces and the disclosed sizes , the microparticles used may not be detected by the endogenous macrophages as foreign bodies so that no or reduced defensive reactions take place . according to a representative embodiment , the microparticles have spherical forms or sphere - like forms capable of forming closely - packed arrangements at the site where they have been implanted and further capable of being individually encapsulated by scar tissue of the host . for instance , the microparticles , which in a representative embodiment may comprise pmma spherical beads , after being inserted into the treatment region , may be encapsulated by delicate capsules of connective tissue and / or embedded into connective tissue or fibers where they remain stationary in the tissue . according to exemplary implementations , regarding maturation of the microparticles , which in a representative embodiment may comprise pmma spherical beads , as a result of the size and physical stability of the pmma beads , they cannot be phagocytised or lysed . in order to isolate the foreign body , the animal body can only fibrotically wall off the foreign bodies in the form of scar tissue . such a process typically takes place with almost any foreign body which cannot be destroyed by the animal body . to the extent present , the fibrotic growth of connective tissue can be a natural reaction to one or more of the lesion of the tissue caused by the injection cannula ( e . g ., needle ) and the presence of the microparticles . the fibrotic reaction may occur , for example , during 3 to 6 months after injection of the biocompatable augmentation or repair material due , for example , to the smooth and chemically inert surfaces of the microparticles ( e . g ., pmma beads ). from then on , the beads can remain in the tissue without reaction and provide for the formation and existence of permanent fibrovascular connective tissue . the microparticles used , according to representative implementations of the present invention , can comprise a polymer , and in particular a completely cured and fully polymerised polymer so that no remaining monomers , which may be toxic or may cause cancer , are incorporated into the body of the treated patient . in principle , it is possible to use any inert histocompatible polymer for producing the microparticles used according to the present invention . modified embodiments may comprise , in whole or in part , non - polymer microparticles . referring to the crosslinking of the native collagen portion of the enhanced carrier , the crosslinking can be accomplished through the addition of energy , or chemical crosslinkers . crosslinking is the linking of collagen molecules by covalent bonding to polyfunctional polymers . traditionally , native collagens have been crosslinked using gluteraldehydes in a concentration of roughly 300 ppm . in one preferred embodiment of this invention , it is preferable to utilize glyceraldehydes to achieve the crosslinking , as that crosslinking agent allows the mixture to maintain all of the desirable properties listed above , along with better extrudability than the other crosslinking agents . while glyceraldehyde is one of the preferred chemical crosslinkers , there are many others such as , but not limited to ; dialdehyde starch , dimethyl adipimidate , carbodiimide , glucosepane , formaldehyde , gluteraldehyde , pentosidine , polyisocyanates , alginates , sulfhydryls , and genipin . in addition , the crosslinking of the native or fibrillar collagen can be achieved through the addition of energy , in forms such as , but not limited to , convective heat , conductive heat , radiation heat , ultraviolet light , near and far infrared energy , irradiation , sonic and ultrasonic energy . the control of the ratios of crosslinked and denatured collagen is an important factor in maintaining consistency of the tissue augmenting material . in a preferred embodiment , the biocompatible carrier comprises between about 30 % to about 70 % crosslinked material . in the preferred embodiment , the crosslinked material will provide a t m sufficient to maintain a gelatinous state and therefore maintain substantially evenly dispersed microspheres within the biocompatible carrier . as described above , the tm will be greater than 37 . 0 ° c . in the preferred embodiment . the determination of the amount of crosslinking can be achieved through several assays , such as but not limited to the use of differential scanning calorimetry ( dsc ), or the use of high performance liquid chromatography ( hplc ) to determine the crosslinker concentrations and proportional degree of crosslinking that has occurred . it should be noted that the biocompatible carrier comprises a “ crosslinked material ” being referred to in the past - tense . for simplicity , the “ crosslinked material ” may be referred to herein as “ terminally crosslinked material ” since the crosslinking reaction is terminated prior to combining with denatured collagen . the crosslinked and gelatinous collagens and microspheres and other agents can be blended into a uniform mixture at elevated temperatures , and then cooled to a semisolid or gelatinous state . the mixture can be stored at room temperatures and beyond if necessary for subsequent introduction ( e . g . injection ) into a patient . generally , various types of processing may be implemented on the enhanced carrier and / or otherwise , at various points in time and / or various locations . for example , the injection apparatus may be operated in conjunction with a micronizing function , which , for example , may be implemented with one or more of structure configured to perform physical micronization ( e . g ., by way of a rotating cutter disposed in the usable volume of the syringe or delivery system barrel , or forcing the material through meshes or small orifices ) and structure configured to perform ultrasonic micronization ( e . g ., by way of application of ultrasonic energy from an external source to the usable volume of the system barrel ), a component of either , and combinations thereof . additionally , or alternatively , any implementation of the syringe or delivery system described herein may be operated in conjunction with a filtering function , such as , for example , an implementation wherein the enhanced carrier ( or , in modified embodiments , tissue , cells and / or fluid ) is passed through a sterile filter ( e . g ., a 0 . 2 micron filter to remove microorganisms ) formed within or in conjunction with a syringe or other delivery system , for example , before mixing with microparticles . some components of the enhanced carrier solution , herein referred to as carrier components , may include agents such as but not limited to , a crosslinking agent , an agent to assist in homogeneity , a coagulant agent , a growth factor , a hemostatic agent , a radiopaque indicator agent , a photosensitive dye agent , a contrasting agent , stem cells , an agent to facilitate bonding , an adhesion prevention agent , an antibiotic agent , an anesthetic agent , an anti - inflammatory agent , a gelatin ( harvested , added in the form of a powder , or in the form of a gelatinous media , any of which can be from the host , synthetic , or from another organism or animal source or vegetable source ), or a combination including one or more of the preceding components , or other materials which are capable of for example being introduced into a second sterile injection syringe and injected through a mixer in parallel with the other components before being injected into the body to achieve a suitable or optimal correction of an issue or problem . the mixing ratio of the components of the enhanced carrier ( e . g ., crosslinked collagen material and gelatinous carrier material and microspheres ) can be controlled and dictated according to the physiological and delivery needs , and in particular according to the density of the tissue and the size of the microspheres used for the injection . for the application or injection of the microparticles used according to an embodiment of the present invention , the microparticles can be 5 % to 80 % of the mixture by weight . the crosslinked collagen component can be 5 % to 90 % of the mixture by weight . the gelatinous component of the mixture can be 5 % to 90 % of the mixture by weight . the administering of the tissue treatment implant can be performed on , for example , humans and animals ( e . g ., horses , dogs , and cats ) and can comprise , for example , treating or augmenting tissue , such as , for example , one or more of augmenting a skin defect and / or cosmetically enhancing a facial feature ; enhancing penile , breasts , or clitoral ; bulking one or more of a vocal cord , a lower esophageal sphincter to control gastroesaphageal reflux disease ( gerd ), a pyloric sphincter , a bladder sphincter and an anal sphincter to relieve urinary or fecal incontinence ; occluding the fallopian tubes , vas deferens , or reproductive organs or passageways to achieve an infertile condition ; repair of vasculature ; repair of the aortic valve , mitral valve , tricuspid valve , pulmonary valve , or other coronary valves ; repair of the heart or other organs ; repair of ulcerous organs or passageways ; occluding a vascular supply to a tumor ; tumor metastasis or tumor embolization by way of , for example , blocking a vascular flow exiting a tumor ; tumor embolization by intratumor application , blocking a vascular supply to a tumor , blocking a vascular flow exiting the tumor , and / or combinations thereof , a repair or augmentation of a vertebral disk ; bulking or repair of muscles , bulking or repair of tendons , bulking or repair of the gums , bulking of the cornea or repair of the optic nerve or other optic anatomy , repair of plantar facia loss in the base of the foot , cartilage augmentation , cartilage repair , synovial fluid replenishment and / or combinations thereof , and administration of the tissue implant to one or more of nasal cartilage , for minimally invasive rhinoplasty or for the treatment of sleep apnea , ear cartilage , elbow , hip , or knee joint cartilage , spinal cord cartilage , and combinations thereof . following insertion ( e . g ., injection ) of the biocompatible augmentation or repair material into a region or regions of interest , the biocompatible augmentation or repair material in accordance with one aspect of the present invention may in certain implementations begin to undergo a complete or at least partial biodegradation of , for example , the biocompatible medium ( e . g ., the enhanced carrier material ). in accordance with a typical implementation , following insertion of the biocompatible augmentation or repair material , the enhanced carrier material is at least partially and , preferably substantially , resorbed into or via tissues of the host mammalian body and / or replaced or supplemented with host tissue ( e . g ., host collagen ). in a representative embodiment , the biocompatible carrier medium is both resorbed and replaced with host tissues . in one aspect of the present invention , a system for delivery of a tissue augmenting material comprises a syringe delivery system and a material for injection . referring to fig1 , in one embodiment of the present inventions , the material for injection 4 can be stored in and delivered by a syringe delivery system . the syringe delivery system can comprise a syringe barrel 2 with attached finger flanges 3 , a plunger and a plunger rod 5 , and a needle 1 . the material for injection 1 can comprise a biocompatible carrier , and an amount of evenly - distributed suspended microparticles . the biocompatible carrier can comprise at least one crosslinked material and a partially - denatured gelatinous collagen and / or other biocompatible gelatinous material . the crosslinked material can be selected or manufactured to provide the material for injection with a t m in the range of about 37 ° c . to about 65 ° c . a t m in this range will allow the material for injection to maintain a gelatinous state in situ as well as during storage of the product , and therefore sufficiently maintain a substantially even distribution of the suspended microparticles without the cost detriment due to a need for refrigeration . additionally , this system will provide a product capable of shipping without the need for refrigeration and time - sensitive delivery . in one aspect of the present invention , a method for manufacturing a material for tissue augmentation comprises , providing a crosslinked material and a gelatinous collagen in an aseptic environment , combining an amount of the crosslinked collagen with an amount of gelatinous collagen or other biocompatible gelatin to form a biocompatible carrier , heating the biocompatible carrier to form a liquid mixture , introducing an amount of microparticles , suspending said particles by rotation or other mixing technique , and cooling the composition below 37 ° c . while this invention has been described with respect to various specific examples and embodiments , it is to be understood that the invention is not limited thereto and that it can be variously practiced . multiple variations and modification to the disclosed embodiments will occur , to the extent not mutually exclusive , to those skilled in the art upon consideration of the foregoing description . additionally , other combinations , omissions , substitutions and modifications will be apparent to the skilled artisan in view of the disclosure herein . for example , alternate means of manufacturing the enhanced carriers , microspheres , cells , or fluids may be employed , and they are not limited to those described above . in addition , the methods of processing ( e . g ., micronizing ) the enhanced carrier , cells , or fluids , and the means of mixing the components of the tissue augmentation and / or repair materials can be varied , or the assisting materials that are preloaded into the delivery system can be reduced or expanded to exclude or include any number of additives or chemical or biological agents . accordingly , the present invention should not be limited by the disclosed embodiments , but is to be defined by reference to the appended claims .