text: the discovery of a differential - diagnostic test / assay , referred to herein as ; oxidation — reduction test ( ort )/( or - test ), or dehydrogenase test ( dgt ), or as more commonly referenced , the test of the author ( brilman &# 39 ; s test ), is based on the reduction of an indicator during incubation of bacteria . this ort , characterizing reduction of the indicator , is constantly negative and constantly positive beside conditionally pathogenic e . coli ( 354b strains ) in semifluid mannitol and in kligler &# 39 ; s medium . in the absence of this necessary laboratory test , genus escherichia and type species e . coli are categorized with non - pathogenic e . coli as well as pathogenic ( epe ). as maladies exist in patients with dysfunction of the bowels , who have constantly negative ort , the need has existed for additional special study that pertain to the genus brilmanella . this assay , which defines the genus , is the first step in an effort to treat maladies of the bowel based on this genus . as a result of laboratory observations , ( on significant material ), the ort , which recognizes differences in characteristics of separate bacteria is discovered and is the basis for a diagnositic to treat patients having maladies based on this bacteria genus . at the core of bacteriological research on differential diagnostics of the representative family enterobacteriaceae , is the recognition of ferments ( herein defined as the simpler substances of the breakdown of an organic molecules ) hydrolases , catalysis hydrolysis of the organic binding , or more simply , hydrolysis breakup of carboyhydrates . previously , diagnostics have not taken into account the group of oxidized - reconstruction ferments , which is the basis of bacteriological breathing and fermentation . in this group , aerobic and anaerobic dehydrogenases exist . the role of these hydrogen acceptors , in the process of oxidation , exist alongside with other materials of different indicators which join with hydrogen reduction and are decolorized . recognition of the reduction of the applicable indicator ( the ferments ) is the basis of the test . further , this test has diagnostic significance with respect to the determination of other ferments , taking into account in laboratory practice : cytochrome oxidate , dehydrocarboxydase , urease , etc . the essence of the test is performed in the following manner : bacteria are incubated on semifluid media , giss and on kligler &# 39 ; s medium . conditionally pathogenic escherichia exhibit typical biochemical processes , under which the indicator , via fixative carbohydrase fermentation , by change of its coloration , first restores the initial color in the aerobic layer of the medium during the process of the incubation and then in the anaerobic layer ( over 48 - 72 h . of the incubation ). the oxidation - reduction process occurs through 20 - 24 hours of growing bacteria on semifluid mannitol and kligler &# 39 ; s medium , illustrating one change of the color of the indicator in its surface layer in contrast with the remaining portion of the column of the nourishing medium . this test was discovered during a study of sick children with dysfunctional intestines by investigating 2886 strains of bacteria e . coli without discovery of epe . nearly all of them ( 99 . 38 %) showed that the dehydrogenase test ( dgt ) was positive with the exclusion of 22 strains ( 0 . 62 %) which are not known serovars of epe ( or to other pathogenic enterobacteria ); wherein complete serotyping was performed including somatic ( o ), capsular ( k ), flagellar ( h ) antigens and also by epe ; the resulting ort being negative . it should be recognized that if under investigation , bacteria undergoing serological typing give a positive result in reaction of agglutination with diagnosing enteropathogenic intestines bacterias “ o ”- and “ ok ”- serums which are or - test positive in the last witnesses culture of contamination , such bacteria need dispelling for reception of the pure culture . in regard to the patients having the excluded 22 strains of bacteria ( 0 . 62 %) with negative or - test , additional study of such bacteria is needed for a variety of pathogenic bacteria which may exist in the genus brilmanella . further , e . coli having haemolytic characteristic with negative or - test may also exist in the brilmanella genus ( these are referred to by the applicant as type “ y ”). moreover , other bacteria found in a patient with dysfunction of the bowels with constantly negative ort , not related to known serovars of epe and “ e . coil ” having hemolytic characteristics with negative or - test , may belong to variants of the bacteria brilmanella genus . additional support for the effectiveness of the assay of the invention is based on experiments conducted with 112 strains of different serological types of epe . results of the experiments found the dehydrogenase test ( dgt ) was constantly negative ( absence recovering the coloration of the indicator ) in column semifluid mannitol and in kligler &# 39 ; s medium . this was also true for salmonellas , shigellas , yersinias and other pathogenic bacterias , for which an absence of dehydrogenase fermentation is typical . research of epe is commonly performed on serologically select growing colonies on nourishing ambience in a petri dish . therefore , it should be recognized that in this incubation environment ( a petri dish , with nourishing substances ), enteropathogenic intestine bacteria do not differ from colonies of conditionally pathogenic e . coli . further , it has been ignored that entereopathogenic intestine bacteria , which are not diagnosed by any currently available set of diagnostics , have a generic or - test that is negative . it is therefore significant to note that of the pathogenic genus of intestine bacteria under investigation , each type of colonies grew on several nourishing ambience in a petri dish , irrespective of the results from the serological research . fermenting incubation characteristics were analyzed after sift incubation was performed ( under 37 ° c . 24 - 27 ( before 48 ) hours ) whereupon a negative or - test was recognized . the constant absence of dehydrogenase fermentation by pathogenic bacteria is a typical test ; differentiating them by use of the assay of the present invention from conditionally pathogenic bacteria allows the selection of epe in a separate pathogenic genus bacteria ; family enterobacteriacea — in the genus brilmanella . it will be appreciated by those skilled in the art that changes could be made to the embodiments described above without departing from the broad inventive concept thereof . it is understood , therefore , that this invention is not limited to the particular embodiments disclosed , but it is intended to cover modifications within the spirit and scope of the present invention as defined by the appended claims .