text: according to the present invention , a fluid sample , such as whole blood , is treated with a precipitating reagent to form clusters of ldl . this precipitating reagent preferably comprises a mixture of large polyanions and divalent cations , and the precipitation reaction is mediated by nucleating particles . the clusters of ldl so formed are surprisingly stable in the presence of cholesterol enzymes . cholesterol enzymes , such as cholesterol oxidase and cholesterol esterase , are then added to the sample . at this point the hdl and vldl lipoproteins in the liquid phase are consumed , while the solid phase ldl clusters remain intact . the ldl is then redissolved by a redissolution agent , and is then analyzed by any conventional means . one convenient method of analyzing the ldl is by enzymatic analysis to form hydrogen peroxide , which is then assayed colorimetrically . the greater the specificity of the precipitating agent for ldl , the more useful it is in the present invention . however , some precipitation of hdl and / or vldl may be tolerable , depending on the diagnostic context . preferably , the lipoprotein precipitates are at least 90 % ldl . alternatively , instead of precipitating ldl and then removing the hdl and vldl , the vldl can be selectively precipitated first . after the vldl has been precipitated from the sample , clusters of ldl are then formed by adding a polyanionic compound , a salt of a divalent metal and a nucleating agent to the sample . appropriate enzymes are then added to consume hdl , and the ldl clusters are treated with a redissolution agent to resolubilize the ldl for determination by conventional means . the polyanions that can be used for forming the ldl clusters can be any polyanions which precipitate lipoproteins which do not interfere with subsequent assays for ldl . among the polyanions that can be used are dextran sulfate , heparin , phosphotungstic acid , and polyvinyl sulfate . the dextran sulfate can be high molecular ( i . e ., molecular weight of from 5 × 104 to 2 × 10 6 ) or short - chained ( molecular weight of from 5000 to 50 , 000 ). the preferred concentration ranges of the polyanions in the reaction mixture are from about 0 . 1 to about 8 grams / liter in the case of high molecular weight dextran sulfate , from about 1 to about 15 grams / liter in the case of short - chained dextran sulfate and heparin , from about 0 . 2 to about 5 grams / liter in the case of polyvinyl sulfate , and from about 0 . 3 to about 6 grams / liter in the case of phosphotungstic acid . the polyvinyl sulfate is a polymer derived from polyvinyl alcohol , of which polymer at least 20 % of the vinyl alcohol groups are sulfated . the molecular weight of the polyvinyl alcohol is not critical as long as it can be crosslinked . this is generally the case at a molecular weight of about 5000 or higher . very favorable results can be obtained at molecular weights in the range of 10 , 000 to 150 , 000 or more . for best results , at least 50 % of the vinyl groups are sulfated . optimum results are obtained wherein at least about 65 % of the vinyl alcohol groups are sulfated . sulfation of the polyvinyl alcohol is preferably carried out using a reaction product of sulfur trioxide or cholorsulfonic acid and a lewis base . particularly suitable is the addition product of pyridine to sulfur trioxide . the sulfation reaction is preferably carried out in dimethyl formamide or formamide at a temperature of between 60 ° and 110 ° c . the polyvinyl alcohol may be crosslinked before or after sulfation , and the crosslinking may be effected either chemically or physically . the divalent cations that can be used in the system of the present invention include the ii - a and ii - b cations , particularly calcium , magnesium and manganese . as with polyvalent anions , the divalent cations may be of any species which has the desired precipitating effect and which does not interfere with the subsequent ldl determinations . these cations can be added in the form of a soluble salt such as a chloride salt . the concentration of the divalent metal ions to be added is preferably from about 10 to about 250 mmole / liter in the reaction mixture . a conventional buffer can be used to buffer in a ph range of from about 6 . 5 to about 8 . 5 , such as mes ( morpholino ethane sulfonic acid ), triethanolamine , mops ( morpholino propane sulfonic acid ) or tris buffer . the invention is not limited to the use of any particular precipitating agent . once the ldl fraction has been separated from the other components in the sample , conventional methods of analysis can be used for the ldl determination . the ldl determination can , for example , take place by saponification with alcoholic potassium hydroxide solution and chemical determination according to liebermann - burchard . however , it is preferred to use an enzymetic determination using cholesterol oxidase ( co ) and a cholesterol ester - splitting enzyme or enzyme system , such as cholesterol esterase ( ce ). in the case of the use of cholesterol oxidase , the determinations can be based upon the amount of oxygen consumed , the amount of cholest - 4 - one - 3 - one formed , or the amount of hydrogen peroxide formed using conventional methods for this purpose . since the determination of bound cholesterol is well known , there is no need to describe it it detail . the invention is not limited to any method of determining a precipitated ldl . the particles that can be used as nucleating agents for forming the ldl clusters can be any nucleating particles that do not interfere with the subsequent assay for ldl . for example , iron oxide , chromium dioxide , stainless steel , silicon dioxide , glass , methyl methacrylate particles , and the like can all be used . these nucleating agents must be insoluble in the liquids used in the assay , i . e ., generally insoluble in water . the particle may be organic or inorganic , and , if inorganic , may be amorphous or crystalline . the inorganic salts may be , but are not limited to , metal salts , such as salts of iron or chromium . among inorganic salts , metal or otherwise , oxide ( including dioxide ) salts are preferred , as they are unlikely to interfere with an oxidative reaction . the particle size is preferably between about 0 . 5 and 200 microns , more preferably 1 - 10 microns . however , to obtain a precipitate of large agglomerates ( e . g ., larger than about 100 microns ) in less than 60 seconds , which is particularly useful with the devices disclosed herein , porous iron oxide with an average diameter of from about 1 to about 10 microns ( reference diagnostics , arlington , mass . ), is most preferred . the nucleating agents can be added in with a mixture of the polyanionic compound and the divalent metal . alternatively , the nucleating agents can be coated with the polyanionic compound or various compounds and added along with the divalent metal to the fluid sample . preferably , the nucleating agent precipitates more than 50 %, more preferably , at least 75 %, still more preferably at least 95 % of the ldl . preferably , substantial precipitation occurs in less than 120 seconds , more preferably less than 60 seconds , still more preferably less than 30 seconds . it is desirable that a good solid pellet be formed and that the pellet be readily redisolved by the redissolving agent . preferably , the nucleating agent stabilizes the precipitate so that less than 20 %, more preferably less than 10 %, is redissolved by cholesterol esterase and / or cholesterol oxidase . the redissolution agent , for redissolving the ldl that was found to be most effective was a mixture of edta and sodium chloride , however , the invention is not limited to this agent . other chaotropic agents and / or surfactants known in the art may be used . when a combination of edta and sodium chloride is used , a solution of 2 . 5 - 6 . 5 % sodium chloride and 0 . 05 - 0 . 10 % edta is especially preferred . other redissolution agents , such as from about 75 - 100 units protease per test and from 50 to 200 mm magnesium chloride per test can also be used . the redissolution agents change the ionic strength of the sample , breaking up the lipoprotein clusters in less than thirty seconds . the redissolution agent may be provided in a liquid or solid ( but solubilizable ) phase . the process of the present invention is particularly well suited for use in devices colorimetrically which register the amount or presence of ldl in a sample . because the reaction of the present invention proceeds quite rapidly , generally within about 120 seconds , a reading can be obtained within a short time , so that the test can be performed in , for example , a physician &# 39 ; s office or a health clinic . the following exemplify two types of devices which can be used with the process of the present invention . of course , these examples are for illustrative purposes only , and not for limitation . referring to fig1 one embodiment of a device according to the present invention is shown at 10 . a fluid sample is introduced to the device at a sample initiation area 20 , and red blood cells are trapped in layer 11 . plasma then enters an ldl precipitation zone , layer 13 . a particle enhanced ldl precipitation reagent 21 in zone 13 causes the ldl to precipitate in large clusters 22 in less than 60 seconds . the enzymes cholesterol esterase ( ce ) and cholesterol oxidase ( co ), together with the surfactants deposited in zone 13 , react with the hdl and vldl to produce hydrogen peroxide . surprisingly , the particle enhanced ldl precipitate 22 does not dissolve and does not react with the cholesterol enzymes , so that only the hdl and the vldl react with the cholesterol enzymes , and the hydrogen peroxide generated thereby is consumed by endogenous catalase in less than ten minutes . zone 14 is a thin sucrose coating and is designed to dissolve in less than ten minutes . an alternative dissolvable coating may also be used . an ldl redissolution zone , zone 15 , contains dried hydroxylamine ( ha ), or an alternative catalase inhibitor , and ldl redissolution agents . when zone 14 is dissolved , both the hydroxylamine and the ldl redissolution agents are mixed with the contents of the ldl precipitating zone 13 , where the catalase is inhibited by hydroxylamine and the ldl clusters are then dissolved by the action of the ldl redissolution agents . the re - dissolved ldl then reacts with cholesterol oxidase and cholesterol esterase , or other agents which catalyze hydrogen peroxide - generating reactions when ldl is available as a substrate , generating hydrogen peroxide which flows through a timing barrier 17 into the measurement zone 16 on the device . the length of the color bar developed in the measurement zone is proportional to the concentration of the ldl in the whole blood sample . more specifically , during the first ten minutes , the reactions in zone 13 are as follows : ## str1 ## during this stage , the ldl ppt is not oxidized by air , despite the presence of co and ce . during the next five minutes , the reactions in layer 13 are as follows : ## str2 ## the hydroxylamine inhibits catalase - mediated enzymolysis of the h 2 o 2 . the device of the present invention can be used with a different enzyme system , as shown in fig2 . in fig2 whole blood is introduced into the device 10 at 20 , the top of layer 11 . red blood cells are trapped in zone 11 , and plasma enters zone 13 . a particle enhanced ldl precipitation reagent 38 in zone 13 causes ldl to precipitate in large clusters 22 in less than 60 seconds . in addition , zone 13 is buffered to about ph 9 , and contains cholesterol esterase , cholesterol oxidase , cholesterol dehydrogenase ( cdh ), redissolution agents , nad , surfactants and cation exchange resins . hdl in the plasma reacts with cholesterol esterase , cholesterol dehydrogenase and napd to produce napdh , while the ldl precipitate remainsunaffected . the ph in zone 13 drops with time because of the cation exchange resins which was placed inside this zone . additionally , as the ph falls from 9 . 0 to 7 . 0 , the cholesterol dehydrogenase becomes inactivated , while the cholesterol oxidase and protease become activated . the ldl precipitate is then dissolved by the protease , and cholesterol esterase and cholesterol oxidase react with ldl to generate hydrogen peroxide . the hydrogen peroxide so generated flows through the timing barrier 17 into the measurement zone 16 . the length of the color bar developed in the measurement zone is proportional to the concentration of the ldl in the whole blood sample . of course , it will be appreciated that the device specifically disclosed herein for use in assaying for low density lipoprotein are not the only devices in which the system for assaying for low density lipoprotein can be used . the system of the present invention can be used with any suitable device which provides a means for forming and trapping clusters of ldl which can be separated from hdl for analysis . a specific precipitation reagent for an undiluted plasma sample can be optimized by varying the concentration of magnesium chloride in the presence of 10 gram / l dextran sulfate ( mw 50 , 000 ). as shown in table 1 , at 75 mm magnesium chloride concentration , only ldl precipitated out of the plasma , while hdl and vldl remained in the plasma . table 1 shows the concentration of cholesterol in plasma after precipitation and slow - speed centrifugation . __________________________________________________________________________500 mm 250 mm 125 mm 75 mm 50 mm 25 mm 10 mm__________________________________________________________________________vldl 1 . 0 1 . 0 3 . 5 43 . 5 43 . 0 43 . 0 42 . 5ldl 1 . 0 0 1 . 0 0 . 5 3 . 0 148 . 0 153 . 5hdl 40 . 0 40 . 1 38 . 9 40 . 4 40 . 0 41 . 0 40 . 3__________________________________________________________________________ it can be discerned from the above that the optimum amount of magnesium chloride for forming a precipitate of ldl ranges from about 75 mm to about 500 mm . in this case , the ldl is precipitated preferentially from the vldl and hdl . however , as shown in example 2 , the ldl precipitates formed are not stable in the presence of cholesterol enzymes such as cholesterol esterase and cholesterol oxidase , which enzymes are used to consume the hdl and vldl from the sample so that these latter lipoproteins do not interfere with the ldl assay . samples of 500 μl plasma with known assayed values of vldl , hdl and ldl were treated with an ldl precipitating reagent with and without nucleating agents ( 40 mg porous iron oxide particles / ml solution ) for one minute . the nucleating agent of choice was porous iron oxide , with average diameters ranging from 1 to 10 microns . after the precipitates were formed , an enzyme reagent containing 4 mg of sodium cholate and 30 units of cholesterol esterase was added to each sample . after three minutes of incubation , the precipitates were spun down at 3000 rpm and the supernatants were assayed for cholesterol values . the data in table 2 show that the ldl precipitate formed with the nuclei remained insoluble in the presence of surfactant and cholesterol esterase , unlike clusters formed in example 1 in the absence of nucleating particles . ______________________________________ ldl + % hdl + vldl enzyme dissolution______________________________________ldl ppt . 65 110 100ldl ppt . w / 64 7 6nuclei______________________________________ not all small particles tested exhibited similar effects for precipitating ldl . particles such as silica and powdered glass worked well to enhance the size of the precipitates , but they did not speed up the formation of the precipitates significantly . by using particles of porous iron oxide , the preferred nucleating agent , large precipitates of ldl clusters form in less than 60 seconds in samples of plasma . other particles were tried as nucleating agents , as described in the above example , with the results shown below : ______________________________________characteristics of nucleating agentparticles for lipoprotein precipitationenhancement completenesstype of pellet ease of of precip - particle size formation redissolution itation______________________________________iron oxides 0 . 6 - 10 good yes 95 - 100 % μm solid pelletiron powder 100 μm solid no 45 - 80 % pelletchromium 0 . 5 - 20 solid yes 80 - 85 % dioxide μm pellettitanium irreg - solid no 70 - 80 % dioxide ular pelletsilica gel 4 - 20 μm poor no 50 - 94 %* pelletsand 200 - 400 poor no 50 - 96 %* μm pelletactivated 38 - 150 good no 75 - 85 % charcoal μm pelletcellulose 6 μm poor no 60 - 80 % pelletkaolin 0 . 1 - 4 μm poor no 75 - 79 % pellet______________________________________ * most runs were 50 %- 60 %. in order to determine the presence and / or amount of ldl in a sample , the clusters formed must be destroyed and the ldl must be redissolved in the solution . redissolution agents are used to redissolve the ldl . in this example , the ldl precipitate obtained in example 2 was redissolved using redissolution agents . the most effective formulation for redissolving the ldl precipitate comprised a mixture of edta and sodium chloride . in one embodiment , for about 400 μl of samples , the final amount of sodium chloride in the sample is from about 2 . 5 - 6 . 5 %, and the amount of edta is from about 0 . 05 - 0 . 12 %. as shown in table 3 , ldl precipitates formed by the nucleated precipitating agents dissolved in less than 30 seconds after the redissolution agents were introduced , and the recovery of lipoprotein in the solution was close to 100 %. preferably , recovery is at least 90 %, more preferably at least 95 %. ______________________________________ldl in sample 197 mg / dlldl in supernatant after ppt . 2 mg / dlldl after re - dissolution of 186 mg / dl ( 95 % precipitates recovery ) ______________________________________ the foregoing description of the specific embodiments will so fully reveal the general nature of the invention that others can , by applying current knowledge , readily modify and / or adapt for various applications such specific embodiments without departing from the generic concept . therefore , such adaptations and modifications are intended to be comprehended within the meaning and range of equivalents of the disclosed embodiments . it is to be understood that the phraseology or terminology herein is for the purpose of description and not of limitation .