Biotechnology, Biology, CRISPR, Virus, Bioinformatics.
polymerase I. The enzyme DNAse creates random cuts or single-stranded nicks in one or both strands of the DNA to leave free 3' hydroxyl groups and 5’ phosphate termini. Now labeled nucleotides that have a fluorophore attached are provided. The free 3' hydroxyl group at the nicking site is used as a