Biotechnology, Biology, CRISPR, Virus, Bioinformatics.
integrate fluorescent proteins in a manner that doesn’t interfere with either dCas9 binding to sgRNA or sgRNA binding to the genomic sequence. We have already discussed in Part 30 that dead Cas9 or dCas9 is a mutant in which both cleavage domains HNH and RuVC of Cas9 are inactivated. Although dCas9