Biotechnology, Biology, CRISPR, Virus, Bioinformatics.
2016; Clow et al., 2020). Like MCP, PUF domain can also be fused to a fluorescent protein while retaining its capacity to bind to PBS (Fig 7). Engineering sgRNA with tandem repeats of PBS makes it possible to label the dCas9-sgRNA complex with multiple PUF fusion fluorescent proteins. Fig 7: