Biotechnology, Biology, CRISPR, Virus, Bioinformatics.
(Tanenbaum et al., 2014; Ye et al., 2017). Fig 6: Tagging dCas9-sgRNA complex by multiple fluorescent proteins through MS2–MCP interactions A second approach, termed Casilio, employs the modification of sgRNA to harbor a unique 8-mer RNA sequence called PUF binding sequence or PBS (Cheng et al.,