Biotechnology, Biology, CRISPR, Virus, Bioinformatics.
can no longer cleave DNA, it can still bind target DNA with the same precision when guided by sgRNA. Labeling dCas9 and sgRNA with fluorescent proteins Let’s now understand how dCas9 and sgRNA can be labeled. The dCas9 can be labeled by fusing it with eGFP fluorescent protein. dCas9-eGFP, when