Biotechnology, Biology, CRISPR, Virus, Bioinformatics.
primer by the polymerase I enzyme. DNA polymerase I enzyme performs 2 activities: · One is 5'-3' exonuclease activity that degrades DNA and removes the native nucleotides from free 5’ phosphate termini in the 5′→3′ direction (Fig 1a and b) · and the other activity of DNA polymerase I enzyme is