Neuroscience, Science, Brain, Research.
before (b) and after © color unmixing (Methods). Scale bars, 100 μm. d Left, example fluorescence image of a 20-μm-thick mouse spinal cord section stained for astrocytes (GFAP; green) and neurons (NeuN; red) with two distinct fluorophores (Alexa Fluor 488 and 633) demonstrating two-color imaging in