Genetics, Covid-19, Vaccines, Research, Dna.
of dCas12a E925A Mxi1 with NLS fusions (pC-Mxi1, pC-Mxi1-NLS, pC-NLS-Mxi1-NLS, pC-NLS, pC-NLS-Mxi1). (B) Repression pattern in strain FR003 with eGFP integrated into INT4 and © strain FR009 with eGFP integrated into INT2. CRISPRi was assessed using two gRNAs targeting the Kl_ENO1 promoter