Genetics, Covid-19, Vaccines, Research, Dna.
(A) Carotenoids production in S. cerevisiae is a multistep pathway catalysed by three enzymes: crtE, crtYB and crtI derived from Xanthophyllomyces dendrorhous. The pathway can be downregulated using dCas12a in sinlgeplex manner with a gRNA or multiplex using single crRNA arrays targeting promoters