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SignalSilence® PLCγ1 siRNA I #6293

This product is discontinued Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® PLCγ1 siRNA I (+) or SignalSilence®PLCγ1 siRNA II #6254 (+), using PLCγ1 (D9H10) XP® Rabbit mAb #5690 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The PLCγ1 (D9H10) XP® Rabbit mAb confirms silencing of PLCγ1 expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.

Gallery: SignalSilence® PLCγ1 siRNA I #6293 

CST recommends transfection with 100 nM PLCγ1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.


SignalSilence® PLCγ1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PLCγ1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.


Quality Control



Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6).

1.  Singer, W.D. et al. (1997) Annu Rev Biochem 66, 475-509.

2.  Margolis, B. et al. (1989) Cell 57, 1101-7.

3.  Kim, H.K. et al. (1991) Cell 65, 435-41.

4.  Wang, Z. et al. (1998) Mol Cell Biol 18, 590-7.

5.  Watanabe, D. et al. (2001) J Biol Chem 276, 38595-601.

6.  Ozdener, F. et al. (2002) Mol Pharmacol 62, 672-9.

Entrez-Gene Id 5335
Swiss-Prot Acc. P19174





 SignalSilence® PLCγ1 siRNA I





