[null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/media/science-education/science-education-thumbs/10219.jpg", "src": "https://cloudflare.jove.com/files/media/science-education/science-education-thumbs/10219.jpg", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/57324_t.png", "src": "https://cloudflare.jove.com/files/thumbs/57324_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/57110_t.png", "src": "https://cloudflare.jove.com/files/thumbs/57110_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/53886_t.png", "src": "https://cloudflare.jove.com/files/thumbs/53886_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/55095_t.png", "src": "https://cloudflare.jove.com/files/thumbs/55095_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/53578_t.png", "src": "https://cloudflare.jove.com/files/thumbs/53578_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/57809_t.png", "src": "https://cloudflare.jove.com/files/thumbs/57809_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/57134_t.png", "src": "https://cloudflare.jove.com/files/thumbs/57134_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/56535_t.png", "src": "https://cloudflare.jove.com/files/thumbs/56535_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/56029_t.png", "src": "https://cloudflare.jove.com/files/thumbs/56029_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/56226_t.png", "src": "https://cloudflare.jove.com/files/thumbs/56226_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/55743_t.png", "src": "https://cloudflare.jove.com/files/thumbs/55743_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/53754_t.png", "src": "https://cloudflare.jove.com/files/thumbs/53754_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/1710_t.png", "src": "https://cloudflare.jove.com/files/thumbs/1710_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/2085_t.png", "src": "https://cloudflare.jove.com/files/thumbs/2085_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/2518_t.png", "src": "https://cloudflare.jove.com/files/thumbs/2518_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/50267_t.png", "src": "https://cloudflare.jove.com/files/thumbs/50267_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/50379_t.png", "src": "https://cloudflare.jove.com/files/thumbs/50379_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/50628_t.png", "src": "https://cloudflare.jove.com/files/thumbs/50628_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/50879_t.png", "src": "https://cloudflare.jove.com/files/thumbs/50879_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/51087_t.png", "src": "https://cloudflare.jove.com/files/thumbs/51087_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/53386_t.png", "src": "https://cloudflare.jove.com/files/thumbs/53386_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/53439_t.png", "src": "https://cloudflare.jove.com/files/thumbs/53439_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/54293_t.png", "src": "https://cloudflare.jove.com/files/thumbs/54293_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/54189_t.png", "src": "https://cloudflare.jove.com/files/thumbs/54189_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/54360_t.png", "src": "https://cloudflare.jove.com/files/thumbs/54360_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/54361_t.png", "src": "https://cloudflare.jove.com/files/thumbs/54361_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/54960_t.png", "src": "https://cloudflare.jove.com/files/thumbs/54960_t.png", "rendered_width": 104}, null, {"document_url": "https://www.jove.com/keyword/fatty+acids+unsaturated", "unformatted_src": "https://cloudflare.jove.com/files/thumbs/55119_t.png", "src": "https://cloudflare.jove.com/files/thumbs/55119_t.png", "rendered_width": 104}, null]
Fatty Acids, Unsaturated: Fatty acids in which the carbon chain contains one or more double or triple carbon-carbon bonds.

An Overview of Alkenone Biomarker Analysis for Paleothermometry JoVE 10219

Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst

Throughout this series of videos, natural samples were extracted and purified in search of organic compounds, called biomarkers, that can relate information on climates and environments of the past. One of the samples analyzed was sediment. Sediments accumulate over geologic time in basins, depressions in the Earth into which sediment flows through the action of fluid (water or air), movement, and gravity. Two main types of basins exist, marine (oceans and seas) and lacustrine (lakes). As one might guess, very different types of life live in these settings, driven in large part by the difference in salinity between them. Over the last few decades, organic geochemists discovered a toolbox of biomarker proxies, or compounds that can be used to describe climate or environment, some of which work in marine environments and some of which work in lacustrine. We turn our attention here to the marine realm and alkenone paleothermometry using the Uk'37 sea surface temperature proxy. The most well-established and widely applied open-ocean biomarker sea surface temperature (SST) proxy is Uk'37. Uk'37 = (C37:2) / (C37:2 + C37:…


Preparation, Purification, and Use of Fatty Acid-containing Liposomes

Lin Jin*1,2, Aaron E. Engelhart*1,3, Katarzyna P. Adamala1,3, Jack W. Szostak1

1Howard Hughes Medical Institute and Department of Molecular Biology and Center for Computational and Integrative Biology, Massachusetts General Hospital, 2Department of Biomedical Engineering, Boston University, 3Department of Genetics, Cell Biology, and Development, University of Minnesota JoVE 57324

Fatty Acid 13C Isotopologue Profiling Provides Insight into Trophic Carbon Transfer and Lipid Metabolism of Invertebrate Consumers

Ralph Menzel1, Rainer Nehring1, Dilara Simsek1, Liliane Ruess1

1Ecology, Institute of Biology, Humboldt-Universität zu Berlin JoVE 57110 JoVE 53886

Hilda Vargas Robles1, Karla Fabiola Castro Ochoa1, Porfirio Nava2, Angélica Silva Olivares3, Mineko Shibayama3, Michael Schnoor1

1Department of Molecular Biomedicine, Center for Research and Advanced Studies of the National Polytechnic Institute, 2Department of Physiology, Biophysics and Neurosciences, Center for Research and Advanced Studies of the National Polytechnic Institute, 3Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies of the National Polytechnic Institute JoVE 55095

Three-dimensional Biomimetic Technology: Novel Biorubber Creates Defined Micro- and Macro-scale Architectures in Collagen Hydrogels

Veronica Rodriguez-Rivera1, John W. Weidner2, Michael J. Yost1

1Department of Surgery - Division of General Surgery, Medical University of South Carolina, 2Department of Chemical Engineering, University of South Carolina JoVE 53578

JoVE 57783 JoVE 57809

Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts

F. Parker E. Laimbeer1, Melissa Makris2, Richard E. Veilleux1

1Department of Horticulture, Virginia Tech, 2Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech JoVE 57134

Saturated Fatty Acids Induce Ceramide-associated Macrophage Cell Death

Yuwen Zhang1, Jiaqing Hao1, Yanwen Sun1, Bing Li1

1Department of Microbiology and Immunology, University of Louisville JoVE 56535

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster

Soda Balla Diop1, Ryan T. Birse1, Rolf Bodmer1

1Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute JoVE 56029

Measuring mRNA Levels Over Time During the Yeast S. cerevisiae Hypoxic Response

Stephen D. Willis1, A. K. M. Nawshad Hossian2, Nathan Evans2, Mark J. Hickman2

1Department of Molecular Biology, Rowan School of Osteopathic Medicine, 2Department of Biological Sciences, Rowan University JoVE 56226 JoVE 55743

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Reza Salehi*1, Stephen C.M. Tsoi*1, Marcos G. Colazo2, Divakar J. Ambrose1,2, Claude Robert3, Michael K. Dyck1

1Department of Agricultural, Food and Nutritional Science, University of Alberta, 2Livestock Research Branch, Alberta Agriculture and Forestry, 3Laboratory of Functional Genomics of Early Embryonic Development, Université Laval JoVE 53754

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning JoVE 1710

Tyler J. Weeks1, Thomas R. Huser1,2

1Center for Biophotonics, University of California, Davis, 2Department of Internal Medicine, University of California, Davis JoVE 2085

Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography (TLC) Coupled with Gas-Liquid Chromatography (GLC)

Zhen Wang1, Christoph Benning1

1Department of Biochemistry and Molecular Biology, Michigan State University JoVE 2518

A Model of Chronic Nutrient Infusion in the Rat JoVE 50267

Free Radicals in Chemical Biology: from Chemical Behavior to Biomarker Development

1ISOF - Bio Free Radicals, Consiglio Nazionale delle Ricerche JoVE 50379

Analysis of Fatty Acid Content and Composition in Microalgae JoVE 50628

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans

Marshall L. Deline1,2, Tracy L. Vrablik1,2, Jennifer L. Watts1,2

1School of Molecular Biosciences, Washington State University, 2Center for Reproductive Biology, Washington State University JoVE 50879

Matthew Rames1, Yadong Yu1, Gang Ren1

1Lawrence Berkeley National Laboratory, The Molecular Foundry JoVE 51087

Isolation and Compositional Analysis of Plant Cuticle Lipid Polyester Monomers

Seamus Jenkin1, Isabel Molina1

1Department of Biology, Algoma University JoVE 53386

Light-driven Enzymatic Decarboxylation

Katharina Köninger1, Marius Grote1, Ioannis Zachos1, Frank Hollmann2, Robert Kourist1

1Faculty of Biology and Biotechnology, Ruhr Universität Bochum, 2Biology and Biotechnology, Delft University of Technology JoVE 53439

Balaji Olety1, Sarah L. Veatch2, Akira Ono1 JoVE 54293

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass

Gregory W. O'Neil1, John R. Williams1, Julia Wilson-Peltier1, Gerhard Knothe2, Christopher M. Reddy3

1Department of Chemistry, Western Washington University, 2Agricultural Research Service, United States Department of Agriculture, 3Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution JoVE 54189

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Sylvie A. Quideau1, Anne C.S. McIntosh2, Charlotte E. Norris1, Emily Lloret3, Mathew J.B. Swallow4, Kirsten Hannam5

1Department of Renewable Resources, University of Alberta, 2Department of Science, Augustana Faculty, University of Alberta, 3Laboratoire Génie Civil et géo-Environnement, Université de Lille, 4Department of Earth and Environmental Sciences, Mount Royal University, 5Forest Ecology & Production, Great Lakes Forestry Centre, Natural Resources Canada JoVE 54360

Original Experimental Approach for Assessing Transport Fuel Stability

Kenza Bacha1, Arij Ben Amara2, Maira Alves Fortunato2, Perrine Wund2, Benjamin Veyrat2, Pascal Hayrault2, Axel Vannier3, Michel Nardin1, Laurie Starck2

1Institut de Science des Matériaux de Mulhouse, 2IFP Energies Nouvelles, Institut Carnot IFPEN Transports Energie, 3Technocentre Renault JoVE 54361

Identification of Fatty Acids in Bacillus cereus

Christian Ginies1, Julien Brillard1,2, Christophe Nguyen-The1 JoVE 54960

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy JoVE 55119