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Neuro-Immune Mechanisms of Anti-Cryptococcal Protection

Recent Insights into the Paradoxical Effect of Echinocandins

Host Soluble Mediators: Defying the Immunological Inertness of Aspergillus fumigatus Conidia
 
Volume 4, Issue 1
 







J. Fungi 2018, 4(1), 2; https://doi.org/10.3390/jof4010002

Role of Hydrophobins in Aspergillus fumigatus

Isabel Valsecchi 1,2, Vincent Dupres 3, Emmanuel Stephen-Victor 4,5, J. Iñaki Guijarro 2, John Gibbons 6, Rémi Beau 1, Jagadeesh Bayry 4,5 , Jean-Yves Coppee 7, Frank Lafont 3, Jean-Paul Latgé 1 and Anne Beauvais 1,*
Aspergillus Unit, Institut Pasteur, 75015 Paris, France
Unité de RMN des Biomolécules, Institut Pasteur, 75015 Paris, France
Centre for Infection and Immunity, Institut Pasteur de Lille-CNRS UMR8204-INSERM U1019-CHRU Lille-Université Lille, 59655 Lille, France
Institut National de la Santé et de la Recherche Médicale, Unité 1138, 75006 Paris, France
Centre de Recherche des Cordeliers, Université Pierre et Marie Curie–Paris 6, Université Paris Descartes, 75006 Paris, France
Biology Department, Clark University, Worcester, MA 01610, USA
Transcriptome et Epigénome, Institut Pasteur, 75015 Paris, France
Received: 29 November 2017 / Revised: 20 December 2017 / Accepted: 22 December 2017 / Published: 24 December 2017
(This article belongs to the Special Issue Fungal Cell Wall)
Full-Text   |   PDF [4606 KB, uploaded 25 December 2017]   |


Resistance of Aspergillus fumigatus conidia to desiccation and their capacity to reach the alveoli are partly due to the presence of a hydrophobic layer composed of a protein from the hydrophobin family, called RodA, which covers the conidial surface. In A. fumigatus there are seven hydrophobins (RodA–RodG) belonging to class I and III. Most of them have never been studied. We constructed single and multiple hydrophobin-deletion mutants until the generation of a hydrophobin-free mutant. The phenotype, immunogenicity, and virulence of the mutants were studied. RODA is the most expressed hydrophobin in sporulating cultures, whereas RODB is upregulated in biofilm conditions and in vivo Only RodA, however, is responsible for rodlet formation, sporulation, conidial hydrophobicity, resistance to physical insult or anionic dyes, and immunological inertia of the conidia. None of the hydrophobin plays a role in biofilm formation or its hydrophobicity. RodA is the only needed hydrophobin in A. fumigatus, conditioning the structure, permeability, hydrophobicity, and immune-inertia of the cell wall surface in conidia. Moreover, the defect of rodlets on the conidial cell wall surface impacts on the drug sensitivity of the fungus. View Full-Text
Keywords: hydrophobin; rodlet; cell wall; Aspergillus hydrophobin; rodlet; cell wall; Aspergillus 

Valsecchi, I.; Dupres, V.; Stephen-Victor, E.; Guijarro, J.I.; Gibbons, J.; Beau, R.; Bayry, J.; Coppee, J.-Y.; Lafont, F.; Latgé, J.-P.; Beauvais, A. Role of Hydrophobins in Aspergillus fumigatus. J. Fungi 2018, 4, 2.


Valsecchi I, Dupres V, Stephen-Victor E, Guijarro JI, Gibbons J, Beau R, Bayry J, Coppee J-Y, Lafont F, Latgé J-P, Beauvais A. Role of Hydrophobins in Aspergillus fumigatus. Journal of Fungi. 2018; 4(1):2.


Valsecchi, Isabel; Dupres, Vincent; Stephen-Victor, Emmanuel; Guijarro, J. I.; Gibbons, John; Beau, Rémi; Bayry, Jagadeesh; Coppee, Jean-Yves; Lafont, Frank; Latgé, Jean-Paul; Beauvais, Anne. 2018. "Role of Hydrophobins in Aspergillus fumigatus." J. Fungi 4, no. 1: 2.





