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RNF20 (D8C2) Rabbit mAb
Primary Antibodies

RNF20 (D8C2) Rabbit mAb #9425 Western blot analysis of extracts from various cell lines using RNF20 (D8C2) Rabbit mAb.

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Gallery: RNF20 (D8C2) Rabbit mAb - #9425 

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Western Blotting Protocol

A. Solutions and Reagents

B. Protein Blotting

A general protocol for sample preparation.

C. Membrane Blocking and Antibody Incubations

I. Membrane Blocking

II. Primary Antibody Incubation

D. Detection of Proteins

Directions for Use:

* Avoid repeated exposure to skin.

posted June 2005

revised November 2013

Western Blot Reprobing Protocol


B. Protocol


revised October 2016

Protocol Id: 10


Immunoprecipitation for Native Proteins


B. Preparing Cell Lysates

C. Immunoprecipitation

Cell Lysate Pre-Clearing (Optional)

D. Sample Analysis

Proceed to one of the following specific set of steps.

For Analysis by Western Immunoblotting

For Analysis by Kinase Assay

posted December 2008

revised April 2018

Protocol Id: 410

Product Usage Information

Specificity / Sensitivity

RNF20 (D8C2) Rabbit mAb recognizes endogenous levels of total RNF20 protein. This antibody does not cross-react with RNF40 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

Species predicted to react based on 100% sequence homology:

Hamster, Bovine, Pig, Guinea Pig, Horse

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val129 of human RNF20 protein.


In mammalian cells, the significance of histone H2B ubiquitination in chromatin epigenetics came from the identification of the budding yeast protein Bre1 (1,2). Together with the ubiquitin-conjugating enzyme Rad6, Bre1 serves as the E3 ligase in the monoubiquitination of the yeast histone H2B within transcribed regions of chromatin (1-3). Subsequently, the mammalian orthologs of yeast Bre1, RNF20 and RNF40, were identified (4,5). These two proteins form a tight heterodimer that acts as the major E3 ligase responsible for histone H2B monoubiquitination at Lys120 in mammalian cells, a modification linked to RNA Pol II-dependent transcription elongation in undamaged cells. Researchers have shown that DNA double-strand breaks (DSBs) are also capable of inducing monoubiquitination of H2B. This process depends upon the recruitment to DSB sites, as well as ATM-dependent phosphorylation of the RNF20-RNF40 heterodimer, thus highlighting a role for this E3 ligase in DSB repair pathways (6). Indeed, investigators have shown that loss of RNF20-RNF40 function promotes replication stress and chromosomal instability, which may constitute an early step in malignant transformation that precedes cell invasion (7).

Entrez-Gene Id


Swiss-Prot Acc.


Data Sheets & Documentation Data sheet (107KB)

For Research Use Only. Not For Use In Diagnostic Procedures.

Upstream / Downstream Explore pathways related to this product.

Upstream / Downstream Proteins

STRING - Known and Predicted Protein-Protein Interactions.

Antibody Guarantee

CST Antibody Performance Guarantee


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