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DMSO enhances electrospray response, boosting sensitivity of proteomic experiments


Hannes Hahne, Fiona Pachl, Benjamin Ruprecht, Stefan K Maier, Susan Klaeger, Dominic Helm, Guillaume Médard, Matthias Wilm, Simone Lemeer, Bernhard Kuster
Nature Methods, 2013, doi:10.1038/nmeth.2610, 10, 989–991 published on 25.08.2013


We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.